Kit for detection of acute T-lymphocytic leukemia naive T cells and application and method thereof

A technology of lymphocytes and leukemia, applied in the field of biomedicine, can solve the problems of increased leukemia cells, unclear, etc., and achieve the effect of high specificity, small sample size, and high sensitivity

Active Publication Date: 2016-01-06
PEOPLES HOSPITAL PEKING UNIV
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Problems solved by technology

Early naive T cells in the thymus expressed CD34 and/or TdT, and the main antigens at that time were TdT and CD7. Therefore, it was considered that when the proportion of TdT+CD7+ naive T cells in the bone marrow or peripheral blood increased, it w

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  • Kit for detection of acute T-lymphocytic leukemia naive T cells and application and method thereof
  • Kit for detection of acute T-lymphocytic leukemia naive T cells and application and method thereof

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[0037] The present invention provides a kit for detecting the expression of LBTC antigen in acute T lymphocytic leukemia, which includes monoclonal antibodies against the following antigen phenotypes: TdT, cCD3, CD34, CD5, CD7, mCD3 and CD45, respectively carrying the following The above-mentioned fluorescent labels: FITC, PE, PerCP-Cy5.5, PE-Cy7, APC, APC-Cy7 and PacificBlue, the fluorescent labels and components of each monoclonal antibody are shown in Table 1 below.

[0038] Table 1 Monoclonal Antibody Information

[0039] .

[0040] The above kit may also include erythrocyte lysate and cell fixation / membrane rupture agent. In this example, red blood cell lysis solution (10×, product number: 555899) purchased from BD Company and fixation / membrane rupture agent purchased from Multisciences were selected. (Cat. No.: GAS006, including 20ml of liquid A and 20ml of liquid B. Liquid A is used to fix cells, and liquid B is used to break membranes, so that antibodies can enter...

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Abstract

The invention discloses a kit for detection of acute T-lymphocytic leukemia naive T cells, application and a method thereof, and belongs to the field of biological medicine. The adopted technical scheme is as below: the kit for the detection of acute T-lymphocytic leukemia naive T cells can be applied to a flow cytometry, and includes monoclonal antibodies aiming at the following materials: TDT, ccd3, CD34, CD5, CD7, mCD3 and CD45. The antibodies respectively have fluorescent labels. The invention also provides the application of the above kit to the preparation of products for diagnosis of acute T-lymphocytic leukemia. The invention has the beneficial effects that: (1) the kit provided by the invention can quickly and accurately conduct analysis and quantification on trace LBTC antigen of all T-ALL patients; and (2) the gating method and multiple antigen markers integral evaluation provided by the kit has high specific degree and high sensitivity.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a special kit for cell phenotype confirmation, in particular to a kit, application and method for analyzing the phenotype of leukemia naive T cells (leukemic blast T cells, LBTC) in acute T lymphocytic leukemia. Background technique [0002] T-lineage acute lymphoblastic leukemia (T-ALL) accounts for 15% and 25% of childhood acute lymphoblastic leukemia (ALL) and adult ALL, respectively, with large heterogeneity. With the optimization of treatment regimens, the induction remission rate and long-term survival rate of children's ALL have been significantly improved, but the prognosis of T-ALL is still poor; the overall 5-year event-free survival of adult ALL is only about 30%, while that of T-ALL is only about 30%. Long-term survival is worse in ALL. Due to the limited detection level of minimal residues at present, recurrence has become the main reason for treatment failure of T-ALL patie...

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Application Information

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IPC IPC(8): G01N33/577G01N33/533G01N15/14G01N1/30G01N1/38
CPCG01N1/30G01N1/38G01N15/14G01N33/533G01N33/577G01N2001/388
Inventor 刘艳荣王亚哲常艳郝乐袁晓英黄晓军
Owner PEOPLES HOSPITAL PEKING UNIV
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