Fluorescence immunochromatographic assay and kit for quantitative detection of creatine kinase isoenzyme (CK-MB)
A technology of fluorescence immunochromatography and creatine kinase, which is applied in the field of medical testing, can solve the problems of expensive instruments, narrow linear range, and low sensitivity of creatine kinase isoenzyme detection, and achieve the effect of improving sensitivity and detection sensitivity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0069] Example 1: Quantitative detection of CK-MB by modifying antibody to quantum dots by chemical cross-linking method and using direct pre-wetting immunochromatography
[0070] (1) Modification of quantum dots and antibodies
[0071] Using human CK-MM and CK-BB as antigens, goat anti-human CK-MM antibodies and CK-BB antibodies can be obtained by immunization. Since CK-MB has two subunits, M and B, the CK-MM antibody and CK-BB antibody can bind to the M and B subunits of CK-MB respectively to form a double-antibody sandwich structure. ,
[0072] Add 60pmol quantum dots with emission wavelength of 650nm, 10μg EDC and 15μg NHS solution and 10-30μg goat anti-human CK-BB antibody solution to pH 7.4 phosphate buffer solution, mix well and react at room temperature for 4h, add 1mg glycine to block. Separating and purifying with a chromatographic column or a chromatographic column to obtain the quantum dot modified by the CK-BB antibody. Similarly, quantum dots modified with goa...
Embodiment 2
[0082] Example 2: Quantitative detection of CK-MB by modifying antibody to quantum dots by biotin-avidin method and using indirect pre-wetting immunochromatography
[0083] (1) Synthesis and modification of quantum dots
[0084] According to the method of Example 1, streptavidin-modified quantum dots were prepared. Similarly, quantum dots modified with goat anti-rabbit antibody were obtained.
[0085] Dilute CK-BB monoclonal antibody to 1mg / ml with 0.1mol / L sodium bicarbonate buffer (pH 8.0), dissolve 1mg N-hydroxysuccinimide biotin (NHSB) with 1ml dimethyl sulfoxide (DMSO), Take 1ml monoclonal antibody solution and add 20-120μl NHSB solution, react at room temperature for 4 hours, add 1mol / L NH 4 Cl, stirred at room temperature for 10 min. Purify by ultrafiltration centrifugation to remove free biotin to obtain biotinylated mAb.
[0086] Streptavidin-modified quantum dots and biotinylated monoclonal antibodies are mixed at a ratio of 1:3 to 1:12 to obtain CK-BB monoclonal...
PUM
Property | Measurement | Unit |
---|---|---|
Emission wavelength | aaaaa | aaaaa |
Emission wavelength | aaaaa | aaaaa |
Emission wavelength | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com