33 results about "Trichome" patented technology

The invention discloses application of a ZaSPL7 gene in regulation and control of hairy bodies and parthenocarpy of plants, and belongs to the technical field of gene engineering. The gene ZaSPL7 for regulating and controlling organ development of the green prickleyash is separated from cDNA of the green prickleyash and is overexpressed in a plant, the number and density of epidermal hair of the transgenic plant are obviously increased, meanwhile, the number of fruit seeds is obviously reduced, even part of plants have seedless fruit phenotypes, and the unisexual setting rate is increased. The results show that the green prickleyash SPL7 gene may coordinate epidermal hair and fruit development by integrating development stage signals, and has important application value in regulation and control of epidermal appendages (such as thorns and hair) formation and apomixis, and the mechanism provides a new idea for simultaneous improvement of plant stress resistance (by reducing the thorns) and fruit commodity (by seedless). Important theoretical basis and technical support are provided for breeding of the green prickleyash, and the method has a wide application prospect.

The invention relates to an NtWUN gene as well as an expression product, an expression vector and application of the NtWUN gene in regulation and control of plant glandular hair development, and aims to solve the technical problems that the development mechanism of different types of epidermal hairs in plants (such as tobacco) is unknown and effective molecular regulation and control measures and utilization ways are lacked at present. According to the invention, an NtWUN gene for negatively regulating epidermal hair development is identified, a gene editing vector and an overexpression vector are constructed, and a gene editing homozygous strain and an overexpression homozygous strain are obtained through screening; tissue expression, MeJA response mode analysis, subcellular localization, plant phenotypic characteristic identification and the like show that the NtWUN can negatively regulate the occurrence of epidermal hair and influence the change of the total density of the epidermal hair, and the NtWUN has important application value in regulating the development process of the plant epidermal hair.

The present invention discloses a method and apparatus for electrostatic separation of trichomes from plant biomass, addressing the limitations of conventional separation techniques. The method relies on differences in electrical properties of trichomes and plant material, utilizing an external electric field. A multi-component electrostatic separation assembly may prepare and separate one or more samples, thereby incorporating a dispensing component, a pipeline component, and a separation chamber comprising at least one electrode assembly. Moreover, the present invention may also provide for electrode assemblies that feature self-cleaning mechanisms.

The present invention relates to polynucleotide comprising a lncRNA gene according to SEQ ID No: 2, or according to a sequence having at least 50% sequence identity to SEQ ID No: 2, and an AP2c gene according to SEQ ID No: 3, or according to a sequence having at least 50% sequence identity to SEQ ID No: 3, which polynucleotide confers an increased density of type IV trichomes. The invention further relates to a plant comprising said polynucleotide and a SlASAT4 gene according to SEQ ID No. 21, or comprising a homologous sequence having at least 99.6% sequence identity to SEQ ID No. 21 wherein the presence of the polynucleotide leads to increased density of type IV trichomes and wherein the presence of said SlASAT4 gene leads to an increased ratio of S4:17 / S3:15 acyl sugars produced in glandular trichomes present on said plant when compared to a control plant lacking the polynucleotide and the SlASAT4 gene. Such a plant also was found to be resistant to Bemisia tabaci. The invention also discloses markers for the identification of the polynucleotide and the SlASAT4 gene and their use for the identification and development of Solanum lycopersicum plants. Also methods for identifying, producing and selecting of plants and seed are disclosed.

A gas jet sieve trichome separation assembly is employed in use to separate trichomes from source plant material, such as separating kief from cannabis source plant material. The gas jet sieve trichome separation assembly, as well as an associated method of its operation, serves to separate and collect trichomes in a manner that has proven more effective and efficient than past techniques, and in a manner that facilitates enhanced automation and that is intended for larger scale production capabilities. Per various implementations, the gas jet sieve trichome separation assembly has a housing, one or more sieves, one or more rotatable arms, one or more actuators, a vacuum source, and a cyclonic separator.

The purpose of the present invention is to provide: a laminated resin sheet in which whitening and loss of tactile sensation are suppressed even when secondary molding is performed; a method for producing the same; and a molded article thereof. A laminated resin sheet according to the present invention comprises: a resin sheet main body having regularly arranged hairy bodies on at least one surface of a base layer and forming a continuous phase without structural boundaries between the base layer and the hairy bodies; and a protective layer that fills the gaps between the hairy bodies and covers the surface of the resin sheet main body on the side having the hairy bodies, the tensile elastic modulus of the protective layer at 20 DEG C being 0.2 MPa or more and less than 2 MPa, and the average thickness of the protective layer being greater than the average height of the hairy bodies.

The application discloses application of GmSAUR46b in regulating soybean yield, light stress resistance and plant height. GmSAUR46b The application discloses that molecular breeding by using the gene can be used for directional improvement of soybean plant type, such as breeding short-stalk anti-lodging varieties, optimizing midrib structure to improve photosynthetic efficiency, regulating trichome density to enhance stress resistance, and has important agricultural application value.

Glandular trichomes are epidermal outgrowths in plants that are the site of metabolic compound synthesis and storage. Modulating the secretion of metabolite compounds in tobacco and tea leaves can alter the taste profile in products made from these plants. The present disclosure relates to methods for modulating secretion from glandular trichomes and compositions comprising altered trichome secretion of metabolites.

The invention discloses lachnum brevipilosum and application of the lachnum brevipilosum, and belongs to the technical field of fungal biology. The strain is separated from Vaccinium dunalianum twigs, can be used as a microbial source of a natural wood flavoring material, and particularly, it is found that the strain can produce (-)-isohorn ene in microbial metabolites for the first time, so that the blank of synthesis research of microbial wood flavoring components is filled up; the application method comprises the steps of strain activation, solid fermentation culture, dynamic collection of headspace volatile matters and the like, and a fermentation product is composite fragrance containing alcohols, olefins and other components and has rich-layered wood-soil fragrance-spicy composite fragrance; the natural wood perfume is produced in a microbial fermentation mode, the problems that plant raw materials are long in growth cycle and unstable in supply can be solved, and the method has the advantages of being controllable in process, not limited by seasons and regions and high in sustainability.

The present disclosure relates to compositions and methods related to tissue-specific promoters and their uses in plants, including tobacco and cannabis. The provided trichome-specific promoters enable the expression of heterologous polynucleotides in trichome tissues.

To provide a method and an apparatus for producing rice husk fuel having high combustibility.SOLUTION: The method for producing the rice hull fuel 1P comprises separating trichomes from a rice hull material containing a rice hull body on which trichomes are formed (S12) and selecting the separated trichomes from a mixture containing the separated trichomes and the rice hull body from which the trichomes are separated (S13). Detaching trichomes from rice hull material (S12) involves moving the rice hull material within a vessel in which a frictional mechanism is located.SELECTED DRAWING: Figure 5

A gas jet sieve trichome separation assembly is employed in use to separate trichomes from source plant material, such as separating kief from cannabis source plant material. The gas jet sieve trichome separation assembly, as well as an associated method of its operation, serves to separate and collect trichomes in a manner that has proven more effective and efficient than past techniques, and in a manner that facilitates enhanced automation and that is intended for larger scale production capabilities. Per various implementations, the gas jet sieve trichome separation assembly has a housing, one or more sieves, one or more rotatable arms, one or more actuators, a vacuum source, and a cyclonic separator.

The application relates to the technical field of plant molecular biology, and particularly discloses a tomato gland-type epidermal hair regulating gene SlWRKY42 and application thereof, wherein the ORF sequence of the gene SlWRKY42 is shown as SEQ ID NO. 1. By regulating the overexpression of the SlWRKY42 gene in a tomato plant, the density of tomato gland hairs is significantly increased, the transcription and expression of an eneyterpene synthase gene (SlTPS12) and a precursor synthesis pathway transgene are significantly up-regulated, and the content of eneyterpene substances is significantly increased. The gene SlWRKY42 provided by the application can regulate the growth and development of tomato gland-type epidermal hairs, and simultaneously regulate the synthesis of eneyterpene substances.

This invention relates to the field of plant molecular breeding technology, and discloses a tomato NOR‑like1 The application of genes in breeding tomato varieties that are easy to deseed and process, the aforementioned NOR‑like1 The gene is an encoding a NAC transcription factor, the coding sequence of which is shown in SEQ ID NO: 1, or a nucleic acid sequence that has at least 90% sequence identity with the sequence shown in SEQ ID NO: 1 and has the function of regulating the development of the trichomes of tomato seed coat; by reducing or eliminating the above... NOR‑like1 Breeding goals are achieved by adjusting gene expression levels or protein function in tomato plants. The resulting tomato plants exhibit the absence of seed coat hairs on the seed surface, preventing adhesion between adjacent seeds, and producing large fruits. This invention fundamentally eliminates seed coat hairs on tomato seeds at the genetic level, and has broad application potential in breeding tomato varieties with easily separable seeds from the pulp and easily separable seeds.

The present invention relates to a method of producing a plant with an increased density of glandular trichomes and / or with an increased production of metabolite(s) comprising a step of introducing at least one genetic alteration that increases activity of PRODERMAL FACTOR2 (PDF2) of said plant, wherein PDF2 preferably has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, SEQ ID NO: 38, SEQ ID NO: 39, SEQ ID NO: 60, SEQ ID NO: 62, SEQ ID NO: 64, SEQ ID NO: 66, SEQ ID NO: 68, SEQ ID NO: 70, SEQ ID NO: 72, SEQ ID NO: 74, SEQ ID NO: 75, SEQ ID NO: 77, SEQ ID NO: 79, SEQ ID NO: 81, SEQ ID NO: 83, SEQ ID NO: 85, SEQ ID NO: 87, SEQ ID NO: 89 and SEQ ID NO: 90.

An electrostatic separation apparatus and method are provided for isolating glandular trichomes from trichome-bearing plant biomass such as cannabis, hemp, or hops. The system may include a vertically oriented particle transport assembly configured to convey a sample through a linear free-fall pathway, a separation chamber with opposing electrode assemblies generating a uniform electrostatic field, and discharge electrodes positioned below the chamber to neutralize residual charge. Airflow regulation may be achieved through laminar diffuser plates and periodic vibration of critical components, including the hopper and diffuser, to prevent clogging and maintain uniform flow. The hopper may further include a circular discharge spout and rounded internal fillets to prevent bridging and ensure consistent powder recirculation. The apparatus achieves high-purity trichome separation through controlled field exposure, aerodynamic stabilization, and charge-neutralized particle discharge, enabling continuous, high-throughput operation with improved yield, reduced maintenance, and enhanced reproducibility across successive processing cycles.

The invention discloses an application of a cucumber CsLSGT gene as a target spot in regulating formation of a silicification layer on the surface of a fruit gland hair. The formation of the silicification layer on the surface of the cucumber gland hair is reduced by reducing the expression quantity of the CsLSGT gene. It is found through screening that the cucumber CsLSGT gene is related to formation of a silicification layer on the cucumber fruit gland hair surface. In a cucumber mutant, the silicon element content of fruit glandular hair is obviously reduced compared with that of a wild type '649'. Meanwhile, a grafting experiment shows that the function of the CsLSGT gene depends on the overground part, which indicates that the reduction of the silicon element on the surface of the glandular hair is mediated by an overground part factor. The gene can be used for screening cucumber fruits with few silicon elements and improving the commodity quality traits of the cucumber fruits.

The invention relates to the technical field of plant detection, in particular to a method for accurately measuring mature hairy bodies on the surface of a cotton leaf surface, which is characterized in that fresh cotton leaves are used as test materials, the optimal parameters of the test environment are that the temperature is 23 DEG C, the humidity is 30%, and different treatment schemes are used according to the difference of the shape, length and density of the hairy bodies. Interference between the back face and leaf margin hair is removed firstly, then liquid nitrogen soaking low-temperature treatment is carried out, water molecules in air are evenly attached to the surface of the leaf hairy body and crystallized, and therefore a clear leaf hairy body image is obtained. The method has the advantages of no need of reagent preparation, short sample preparation time, clear imaging, clear leaf hairy number and length characteristics, and substantial improvement of the metering efficiency of the mature hairy bodies on the surfaces of the cotton leaves. An efficient and reliable metering means is provided for related breeding work such as morphological research, insect-resistant mechanism analysis and developmental regulation pathway function verification related to cotton leaves, and the problem that leaf hairy bodies, especially dense hairy bodies, cannot be accurately metered is solved.

This invention relates to the field of trichome separation equipment technology, and more particularly to a plant trichome separation device. The technical solution includes a box, a fixing frame, and a separation assembly. An inlet pipe is fixedly installed on the left side of the box, extending into the interior of the box. A recycling bin is slidably installed on one side of the bottom of the box. This invention uses a hollow, cone-like roller structure to tumble the plants, allowing them to roll and separate internally while gradually moving to one side. After separation, the plants automatically move to the waste bin for discharge, preventing the accumulation of large amounts of plant material from affecting subsequent separation. Furthermore, by adjusting the roller's rotation speed, the separation effect of the trichomes can be adjusted through centrifugal force. By introducing high-pressure gas or refrigerant into the plastic film and mounting base, the plastic film expands, opening the cone-shaped roller to contact the plants, increasing the friction on the plant surface, thereby promoting efficient trichome separation.

The present application relates to a method for regulating expression of a target gene in plant secretory trichomes by using a lipid transfer protein gene promoter, a vector used in the method and its application in genetic engineering breeding of plant trichome tissue for expression and production of metabolites. The promoter is the promoter of AaLTP1 gene, which can regulate specific expression of a target gene in plant secretory trichomes. The present application also relates to the use of the aforementioned gene promoter in genetic engineering breeding of plant trichome tissue for expression and production of metabolites. Since the promoter for specific expression in plant trichomes does not harm the growth and development of plants when the trichome system of plants is genetically manipulated, the present application is of great significance in genetic engineering breeding of plant trichome tissue for expression and production of metabolites.

The invention belongs to the technical field of plant molecular biology, and particularly relates to a cotton NAC transcription factor GhNAC71A12 gene, a promoter of the GhNAC71A12 gene and application of the promoter. The invention provides a cotton NAC transcription factor GhNAC71A12 gene. The nucleotide sequence of the GhNAC71A12 gene is as shown in SEQ ID NO: 1, and the nucleotide sequence of the GhNAC71A12 gene is as shown in SEQ ID NO: 2. The gene can regulate and control the length of cotton fibers; the invention also provides a promoter of the GhNAC71A12 gene, and the nucleotide sequence of the promoter is as shown in SEQ ID NO: 3; the promoter can start expression of GUS at Arabidopsis thaliana leaf fur.

The invention discloses a compound regulator for promoting sandalwood aspirator development. Every 100 parts of the compound regulator comprises the following components in parts by weight: 1-3 parts of naringenin, 1-5 parts of melatonin, 5-10 parts of coumarin and the balance of a milk powder carrier. The compound sandalwood sucker accelerant disclosed by the invention can obviously promote the development of sandalwood suckers and improve the afforestation quality of artificial sandalwood forests.

This invention discloses a gene regulating plant glandular trichome development. NtJAZ11, This application aims to address the current technical problem of unclear developmental mechanisms and lack of effective regulatory methods and utilization pathways for glandular trichomes and protective trichomes in plants (such as tobacco). The gene encoding this gene, its expression vector, and its applications are described. NtJAZ11 Overexpression and gene-editing vectors were constructed, and corresponding overexpression and gene-editing lines were obtained through transformation. Subcellular localization, tissue expression, MeJA response pattern analysis, and plant phenotypic identification showed that... NtJAZ11‑2 It can negatively regulate the development of tobacco glandular hairs, and has important application value in regulating the development of plant epidermal hairs.

This invention application discloses a gene regulating plant glandular trichomes. NtMIXTA This application relates to genes, their encoded proteins, biological materials, and applications, aiming to address the technical problem of low efficiency in increasing plant glandular trichome density using traditional breeding techniques. This application clones a gene from tobacco... NtMIXTA Genes, and constructed separately NtMIXTA Gene knockout and overexpression lines were found NtMIXTA Gene knockout reduced leaf glandular hair density, while overexpression lines showed a significant increase in leaf glandular hair density, indicating that... NtMIXTA Genes positively regulate the occurrence and development of glandular trichomes in tobacco. This application is of great significance for studying the molecular regulatory network of plant glandular trichome formation.

The application discloses an LjCYCD2 gene and application thereof in regulating plant leaf trichome density, and belongs to the technical field of genetic engineering.The amino acid sequence coded by the LjCYCD2 gene is shown as SEQ ID NO:2, and the nucleic acid sequence thereof is shown as SEQ ID NO:1.The gene is expressed in Lonicera japonica leaves, and the expression amount gradually decreases with the maturity of the leaves;after the LjCYCD2 gene of the Lonicera japonica leaves is silenced by VIGS, the trichome density of the Lonicera japonica leaves is obviously reduced.A plant overexpression vector of the LjCYCD2 gene of the Lonicera japonica is constructed, and is transformed into tobacco, and the obtained transgenic tobacco leaves have an obviously increased number of trichomes.It can be seen that the LjCYCD2 gene of the Lonicera japonica is related to the development of plant leaf trichomes, and has important application value in regulating the trichome density on the surface of plant leaves.

The invention discloses application of an NtHCH5 gene in regulation and control of plant glandular hair development, and belongs to the technical field of plant genetic engineering. The nucleotide sequence of the gene for regulating and controlling plant glandular hair development provided by the invention is as shown in SEQ ID NO. 1. An RNAi carrier of the NtHCH5 gene is constructed through an RNAi technology, a tobacco plant silenced by the NtHCH5 gene is cultivated, and it is found that compared with a normal tobacco plant, the density of glandular hairs in tobacco leaves after the NtHCH5 gene is silenced is remarkably reduced, and the glandular hairs are shortened. An economic plant tobacco is used as a research object, and experiments prove that the NtHCH5 gene participates in regulation and control of plant glandular hair development, a new target gene is provided for regulation and control of plant glandular hair development, and a gene regulation and control network of plant in-vivo epidermal hair is enriched. The gene function of the plant NtHCH5 gene is also enriched, and the gene has important significance for analyzing the diversity of the plant NtHCH5 gene.

Disclosed is an apparatus and method used to isolate organic acid forms of cannabinoids from a source biomass. This includes the use of a continuous, flow driven, biomass agitation unit, referred to herein as the Continuous Trichome Separator (CTS) Unit. Biomass is fed into the CTS Unit, where trichomes are shaken off of the biomass, and a mixture of trichomes and fluid continue for further processing. The trichomes are melted and then separated using chromatographic separation techniques. Thereafter the organic acids are dehydrated and become isolate compounds.

To provide an electrode for measuring the leaf surface potential of a plant, capable of closely adhering to the leaf surface even in the presence of hair-like projections. The present invention provides: SOLUTION: The electrode for measuring a plant leaf surface potential is composed of a conductive nano thin film including an elastomer layer and a carbon nanotube layer laminated on at least one surface of the elastomer layer.SELECTED DRAWING: None

This invention discloses a tea tree CsMYB82 Genes and their applications belong to the field of genetic engineering technology. Tea tree CsMYB82 The nucleotide sequence of the gene is shown in SEQ ID NO.1 of the sequence listing; the amino acid sequence encoding the protein is shown in SEQ ID NO.2. Studies have shown that... CsMYB82 The gene expression pattern is negatively correlated with the distribution of epidermal hairs (tea trichomes) in tea plants. Overexpression of this gene in plants can inhibit epidermal hair formation; inhibition can be achieved through antisense oligonucleotide technology. CsMYB82 CsMYB82 The expression of this gene can relieve its inhibition of key downstream factors, thereby promoting the development of epidermal hairs or maintaining the density of tea hairs. Cloning this gene is not only helpful in elucidating the negative regulatory mechanism of epidermal hair development in tea trees, but also provides a theoretical basis and genetic resources for the improvement of epidermal hair traits and the breeding of new varieties in tea trees.

The invention discloses application of GmSAUR46b in regulation and control of soybean yield, light stress resistance and plant height. The invention finds that soybean plant types can be directionally improved by using the GmSAUR46b gene for molecular breeding, such as cultivating dwarf lodging-resistant varieties, optimizing midrib structures to improve photosynthetic efficiency, regulating hairy body density to enhance stress resistance, and the GmSAUR46b gene has important agricultural application value.