108 results about "Trichome" patented technology

Novel plant derived regulatory sequences and constructs and methods of using such sequences for directing expression of exogenous polynucleotide sequences in trichomes are provided

A layered fibrous structure having a machine direction, cross machine direction, and Z-direction. The fibrous structure also has a consumer side and first and second layers in the Z-direction where the first layer has a plurality of trichomes. The trichomes make up greater than about 0.1%, by weight, of the fibrous structure.

The invention concerns a method for producing terpenes of interest in plants having glandular trichomes, as well as plants useful for producing said terpenes of interest. Said plants comprise a sequence encoding a heterologous terpene synthase under the control of a promoter enabling it to be specifically expressed in the trichomes. Moreover, the pathway for producing endogenous diterpenes is preferably blocked in the trichomes of the plants, to increase the flow in the heterologous pathway. The secretion of heterologous terpenes is spontaneous resulting in easy collection. The present invention also concerns plants exhibiting a blocked production of a compound having antibiotic properties at the surface of leaves exhibiting enhanced efficiency of transformation by a bacterium.

Individualized trichomes, methods for individualizing trichomes, chemical derivatives of individualized trichomes, trichome-containing fibrous structures, single- or multi-ply sanitary tissue products comprising such fibrous structures and methods for making such fibrous structures and sanitary tissue products are provided.

A horizontal axis rotary separation apparatus is deployed in a process for separating resinous trichomes rich in flavoring, aromatic and/or medicinal components produced in plant trichome glands from unwanted plant matter. The process physically separates resin rich beads at the trichome gland head from extraneous plant matter by one or move separation sieves. The sieves are provided in or as a casing over a rigid frame member. The sieves are mesh fabric bags or screen that are easily opened and replenished in a batch operating mode, and are removable from the frame for cleaning and maintenance. Other aspects of the invention include processes that improve process efficiency and speed, and yield products of superior quality.

A process for trichome separation from plant matter deploys water slurry agitation and/or dry sieving to produce a mixture of solid trichomes and similar sized plant debris. The plant debris in each separate fraction is then removed by gravity filtration in a dense inert liquid, which is preferably a salt or brine solution. The desirable trichomes or trichome glands float on the dense liquid, while the undesirable vegetative matter sinks to the bottom of the brine containing vessel. The trichomes are readily removed and washed before further processing.

A rotary separation apparatus is deployed in a process for separating resinous trichomes rich in flavoring, aromatic and/or medicinal components produced in plant trichome glands from unwanted plant matter. A liquid freezing agent is introduced into a container of the plant matter to fragment the undesirable matter while the desired portion rich in trichome remain intact.

The invention discloses a semi-leaf laver seedling cultivating method. The method includes the following steps that semi-leaf laver nutritious trichome is obtained; the semi-leaf laver nutritious trichome is cultivated in nutritious liquid until a protonema of a sporangium branch with the set proportion is formed and the cultivating conditions include that the temperature ranges from 20 DEG C and 30 DEG C, the light intensity ranges from 1000 lx and 2600 lx, the lighting period is 10 hours to 16 hours and the nutritious liquid sea water ratio ranges from 1.026 to 1.031. The protonema of the sporangium branch with the set proportion is placed on an attachment basal body and stimulated by water currents until conchospores of which the density reaches the seedling collecting requirement are formed and seedling cultivating of the semi-leaf laver is finished. Thus, the problem that the seedlings of the semi-leaf laver can not be rapidly and efficiently cultivated with the prior art is solved.

Trichomes, lupulins and other plant parts can be efficiently separated from plant biomass by continuous filtration. In some embodiments plant biomass is collected and combined with water to form a plant biomass mixture. The plant biomass mixture is passed through a filtration device in which the plant biomass mixture is continuously flowed through a filter material. The device includes a cleaning apparatus that cleans the filter material during the process. In some embodiments greater than 90% of the plant parts are collected from the starting plant biomass. The plant parts can be subsequently processed to obtain desired compounds. For example, trichomes can be separated from other plant biomass and subsequently processed to extract one or more cannabinoids or terpenes, such as THC and/or CBD.

Methods for producing heterologous terpenes, terpenoids and/or small molecules in transgenic glandular trichome-bearing plants are provided, as well as the transgenic glandular trichome-bearing plants capable of producing the heterologous terpenes, terpenoids and small molecules. The genetically engineered glandular trichome-bearing plants contain and express one or more genes which encode proteins active in the biosynthetic pathways which produce the terpenes, terpenoids and small molecules. As a result, the essential oil of the transgenic plant is enriched for the heterologous or homologous terpenes, terpenoids and/or small molecules Storage of the essential oil in the glandular trichomes of the plant reduces the volatility and cytotoxic capacity of the heterologous molecules, thereby increasing yield and decreasing damage to the transgenic plant.

The invention discloses a gene promoter, belonging to the field of plant biotechnology. The nucleotide sequence of the promoter is as shown in SEQ ID NO. 1, and the promoter is capable of regulating the specific expression of target genes in T shape trichome of a plant. Meanwhile, the invention further relates to application of the gene promoter in genetic engineering breeding which realizes expression of a gene in trichome tissues of the plant and produces metabolites. When the promoter realizing specific expression of the gene in trichome of the plant is used for carrying out genetic manipulation on the trichome system of the plant, no harm is caused to the growth or development of the plant; therefore, the promoter disclosed by the invention has an important significance for the genetic engineering breeding which realizes expression of the gene in trichome tissues of the plant and produces metabolites.

Processes for extracting trichomes from plants and more particularly to processes for extracting trichomes from a mixture of trichome and non-trichome materials using a screen, for example a pressure screen, and fibrous structures employing such extracted trichomes are provided.

The present invention relates to a dry process for isolating clean glandular trichomes from a trichome bearing plant material, wherein the trichome bearing plant material is frozen at −20° C. and subject to shattering and softening sequence.

A paraquat resistance gene and a vascular tissue- and trichome-specific promoter are provided. The paraquat resistance gene and the vascular tissue- and trichome-specific promoter are isolated by identifying and analyzing genes of Arabidopsis thaliana.

The invention discloses a tobacco trichome (TTR1) promoter as well as an expression vector and application thereof. The nucleotide sequence of the TTR1 promoter is SEQ ID NO.1. The eukaryotic expression vector designed for plant genetic transformation has a TTR1 promoter sequence; the TTR1 promoter is applied to promotion of target gene expression, a development regulation mechanism for tobacco trichome tissues, formation of aroma substances in the trichome and improvement of tobacco quality; a method for promoting GUS gene expression by the TTR1 promoter is designed; a promoter is inserted into an expression vector containing a GUS gene so as to change a conventional promoter in the expression vector to obtain recombinant plasmid; the recombinant plasmid is transported into a receiver plant and is cultivated. The TTR1 gene promoter disclosed by the invention can drive the GUS gene to be specifically expressed in the tobacco trichome, can be used as an expression exogenous gene of plant genetic transformation and an expression element for a tobacco bioreactor, and has important value for development regulation of the tobacco trichome tissues, formation of the aroma substances in the trichome and improvement of the tobacco quality.

Improved processes for liberating trichome fibers from non-seed portions of a trichome-bearing host plant are provided. The processes include one or more steps for disassociating trichome fibers that are already separated from leaves, stems, and still-attached fibers so that the separated trichome fibers can be collected and not lost through a waste stream.

The invention discloses Artemisia annua L. PDR sub-family transport protein. Amino acid sequences of the protein comprise the amino acid sequence shown as SEQ ID NO:2 or the amino acid sequence of the protein is shown as SEQ ID NO:2 or nucleic acid codes crossed by the protein and complementary chains of nucleic acid of protein with the coded amino acid sequence shown in SEQ ID NO:2 under the highly-strict condition are achieved. Furthermore, the protein is named AaPDR1 by the inventor and is transport protein specifically expressed by Artemisia annua L. secreting type gladular trichomes, and it is proved through a yeast transport experiment and interference of Artemisia annua L. in the transport protein that the AaPDR1 participates in transporting an artemisinin synthetic route intermediate product dihydroartemisinic acid in Artemisia annua L.. The Artemisia annua L. PDR sub-family transport protein has great significance in providing high-yield and stable new drug resources for large-scale production of artemisinin.

The invention discloses a gene promoter, belonging to the field of plant biotechnology. The nucleotide sequence of the promoter is as shown in SEQ ID NO. 1, and the promoter is capable of regulating the specific expression of target genes in T shape trichome of plants. Meanwhile, the invention further relates to application of the gene promoter in genetic engineering breeding which realizes expression of a gene in trichome tissues of the plant and produces metabolites. When the promoter realizing specific expression of the gene in trichome of the plant is used for carrying out genetic manipulation on the trichome system of the plant, no harm is caused to the growth or development of the plant; therefore, the promoter disclosed by the invention has an important significance for the genetic engineering breeding which realizes expression of the gene in trichome tissues of the plant and produces metabolites.

The invention provides a gene promoter, belonging to the field of plant biotechnology. The gene promoter has a nucleotide sequence as shown in SEQ ID No. 1. The AaTCP15 gene promoter can regulate theexpression of a target gene in all the tissue of a plant, especially preferential expression in secretory glandular trichomes and non-secretory glandular trichomes. Meanwhile, the invention also relates to application of the AaTCP15 gene promoter to genetic engineering breeding which employs the glandular trichome tissue of plants for expression and production metabolites. Since genetic manipulation of the promoter preferentially expressed in glandular trichomes on the glandular trichome system of plants has no harm to the growth and development of the plants, the AaTCP15 gene promoter provided by the invention is of critical significance to genetic engineering breeding which employs the glandular trichome tissue of plants for expression and production metabolites.

Described is the specific delivery of agrochemicals to plants. More specifically, described is a composition having a targeting agent comprising at least one binding domain that specifically binds to a binding site on an intact living plant and an agrochemical or a combination of agrochemicals. Also described is a binding domain that specifically binds the binding site on an intact living plant. More specifically, described are binding domains comprising a peptide sequence that comprises four framework regions and three complementary-determining regions, or any suitable fragment thereof, so that the binding domains are able to bind or retain a carrier onto a plant. Described are binding domains that specifically bind trichomes, stomata, cuticle, lenticels, thorns, spines, root hairs, or wax layer. Described is a method for delivery of agrochemicals to a plant, for improving the deposition of agrochemicals on a plant, and for retaining the agrochemicals on a plant, using targeting agents comprising the binding domains, and to a method for protecting a plant against biotic or abiotic stress or controlling plant growth using the same. Also, described is a method for manufacturing a specifically targeting agrochemical carrier.

A process for trichome separation from plant matter deploys an ionic brine to induce oedemic transformation of disk cell to aid in releasing glandular trichomes. The brine can be agitated to further release disk cells from the plant tissue. The disk cell debris can be separated by sieving the larger trichomes and plant tissue particles that results from agitation and maceration. If the brine concentration is appropriately modified, the trichomes will float and the brine and the plant tissue fragments sink. The floating trichomes are then removed and rinsed and then dried or extracted further after drying.

The present invention relates to processes for individualizing trichome fibers from a trichome source, such as a leaf and/or a stem. One process comprises contacting the plant biomass with pectin hydrolyzing enzymes, thus releasing the individualized trichomes and recovering the individualized trichomes. A second process comprises contacting the plant biomass with an acidic aqueous solution, thus releasing the individualized trichomes and recovering the individualized trichomes. A third process comprises contacting the plant biomass with an acidic aqueous solution and with pectin hydrolyzing enzymes, thus releasing the individualized trichomes and recovering the individualized trichomes.