21 results about "Light stress" patented technology

The invention provides an automatic illumination adjusting method and system based on seed and seedling cultivation, and relates to the technical field of illumination control and intelligent agriculture, the method comprises the following steps: firstly, collecting seedling chlorophyll fluorescence parameters to construct a light stress index, and accurately identifying a light inhibition risk; then calculating a physiologically permissible light intensity threshold adaptive to the dark response capability of the plant by combining the environment temperature and the CO2 concentration; positioning a shielded weak light area by using three-dimensional point cloud voxelization and a ray tracing technology, and calculating a shadow compensation gain coefficient; and finally, generating a control matrix in combination with a dynamic left-rotation light strategy, and driving an independent addressing LED array and a variable-angle lens to execute local precise light supplement and light vector adjustment. Through physiological feedback and spatial light field reconstruction, the problem that traditional illumination control neglects individual tolerance difference and canopy shielding is solved, photooxidation damage is effectively prevented, and the growth uniformity and the light energy utilization rate of seedling groups are remarkably improved.

The invention relates to the technical field of facility agriculture and environment control, and discloses an intelligent illumination control system for greenhouse plant growth. In order to solve the problems that an existing light supplementing strategy lacks physiological feedback, light damage is easily caused, and shutdown is caused by sensor faults, the system comprises a multi-mode sensing assembly, a central processing unit and a dynamic spectrum execution assembly. The system collects crop non-photochemical quenching coefficients (NPQ) and environmental factors in real time, and dynamically calculates light saturation points by using a multi-factor coupling model; when the NPQ exceeds a threshold value, the spectrum ratio is automatically adjusted to inhibit light stress; three-dimensional canopy scanning is combined to realize gridding differentiation light supplement; when the sensor fails, virtual parameters are reconstructed based on the temperature trend to maintain fault-tolerant operation; in addition, the system estimates stomatal conductance according to air humidity and matrix conductivity, and dynamically corrects target light intensity to avoid aggravation of water deficit; accurate light supply according to needs is achieved, the photosynthetic efficiency and the system reliability are remarkably improved, and the system is suitable for plant factories and multi-span greenhouses.

The ecological toad culture pond comprises a pond body, water tanks are formed in the two sides in the pond body respectively, shallow grass layers are arranged on the peripheries of the water tanks, intercepting nets are arranged in the water tanks, lifting frames are arranged on the two sides of the intercepting nets respectively, the lifting frames are connected with mounting bases, supporting frames are inserted into the mounting bases, and the supporting frames are connected with the water tanks through the lifting frames. The two sides of the pool body are respectively provided with a lifting mechanism, the two lifting mechanisms are respectively connected with the corresponding supporting frames, a sunshade inhabitation area is arranged between the water tanks on the two sides, and a boss is arranged on one side in the pool body, so that toad cortex naturally falling off from toads can be effectively intercepted and collected, the toad cortex is prevented from being lost along with water flow, and the toad cortex is prevented from falling off. In addition, the sun-shading inhabiting area is arranged, so that direct strong light can be effectively blocked, a proper environment is created for the toads, abnormal ecdysis of the toads caused by strong light stress is reduced, and the toad cortex quality is effectively guaranteed.

The application discloses application of GmSAUR46b in regulating soybean yield, light stress resistance and plant height. GmSAUR46b The application discloses that molecular breeding by using the gene can be used for directional improvement of soybean plant type, such as breeding short-stalk anti-lodging varieties, optimizing midrib structure to improve photosynthetic efficiency, regulating trichome density to enhance stress resistance, and has important agricultural application value.

The application provides a gene and an application thereof CaCA2 The application belongs to the technical field of gene breeding and relates to application of a gene in pepper resistance to low-temperature and weak-light stress. CaCA2 The application discloses a gene and an application thereof CaCA2 The transient overexpression of the gene can slow down wilting of pepper plants, improve net photosynthetic rate of pepper leaves, expression amount of antioxidant enzyme genes, soluble sugar, free proline and mineral element content, and simultaneously reduce intercellular CO2 concentration and malondialdehyde content. CaCA2 After the VIGS technology is used to transiently silence the gene, the plant height, stem diameter, biomass and root morphological parameters of the pepper are reduced in varying degrees, and the gene causes stoma to close, and the overall fluorescence intensity, photosynthetic performance index and trace element content are reduced. CaCA2 The transient overexpression of the gene in the pepper can relieve wilting of the pepper plants and improve the resistance to low-temperature and weak-light stress, and the resistance is weakened after the gene is silenced.

The application discloses a method for enhancing the low-temperature and weak-light stress resistance of melons, relates to the technical field of cultivating melons with enhanced low-temperature and weak-light stress resistance, and aims at solving the problem of imperfect screening and cultivation of low-temperature and weak-light stress resistance genes of melons during cultivation. The application can timely find the signs that melons are stressed through low-temperature and weak-light stress monitoring, can optimize the growth environment and management measures of melons through a scientific growth cycle management plan, thereby improving the yield and quality of melons, and can significantly improve the adaptability of melons to low-temperature and weak-light stress. The expression amount analysis result can be associated with standard low-temperature and weak-light transcription factors in a database to screen key regulation genes related to low-temperature and weak-light stress resistance. The melons with stress resistance phenotypic characteristics screened can help to promote the improvement and upgrading of melon varieties. The cultivation environment can be accurately adjusted to improve the growth conditions of melons, thereby enhancing the resistance of melons to low-temperature and weak-light stress.

This invention provides CaCA1 The application of genes in the resistance of chili peppers to low temperature and low light stress falls under the field of gene breeding technology. Under low temperature and low light stress... CaCA1 Transient overexpression can slow down wilting in pepper plants, increase the content of photosynthetic pigments, transpiration rate, antioxidant enzyme activity and their gene expression levels, soluble protein, free proline and mineral element content in pepper leaves, and reduce intercellular CO2 concentration, malondialdehyde and heat dissipation per unit area. Transient silencing was performed using VIGS technology. CaCA1 Genetic factors reduced the plant height, stem diameter, biomass, and root morphology parameters of chili peppers, and caused stomatal closure, resulting in a decrease in overall fluorescence intensity, photosynthetic performance index, and element content. CaCA1 Transient overexpression of the gene in peppers can alleviate plant wilting and improve resistance to low temperature and low light stress, while silencing the gene... CaCA1 Genetic modification can weaken its resistance.

The invention discloses a high-light-resistant algal strain high-speed screening method and system based on micro-fluidic single cell sorting, and relates to the field of bioengineering.The high-light-resistant algal strain high-speed screening method comprises the steps that haematococcus free cells in the logarithmic phase are taken and added into a double-fluorescence probe mixed solution, and incubation is conducted in a dark place to obtain a haematococcus solution; bBM sheath fluid and haematococcus fluid are introduced into a sample introduction area of the micro-fluidic chip, the BBM sheath fluid and the haematococcus fluid are focused by the sample introduction area and then enter a highlight stress area, highlight treatment is carried out to induce motile cells to be transformed into spores, and the transformed cells enter a detection area. Through in-situ high light stress and single cell continuous sorting of the micro-fluidic chip, the sorting efficiency is high, and the screening period is greatly shortened; three parameters of chlorophyll fluorescence, astaxanthin fluorescence and forward scattering light are adopted for cooperative detection, and a threshold value which is quantitatively set based on a control group is combined, so that the proportion of a false positive strain is reduced to be less than 5%, and the screening accuracy of a double-superior strain is greatly improved; the microfluidic chip is extremely low in single reagent consumption, greatly reduces the screening cost of single strains, and is easy for large-scale popularization and application.

The application discloses a kind of composite toughened glass stress distribution uniformity monitoring methods, comprising: by polarized light stress scanner, continuous scanning is carried out along the glass transmission direction, the stress value of each position on glass surface is collected, while the measured angle of scanning path and roll direction is recorded, and the glass thickness and roll diameter are measured, according to the glass thickness, the glass to be measured is divided into thin glass or thick glass;According to the measured angle of scanning path and roll direction, the stress value of each position on glass surface is converted, the stress value obtained by oblique scanning is uniformly mapped to the glass transmission direction, and the stress distribution record arranged along the transmission path is formed;The bending arc profile and stress transition uniformity of thin glass are comprehensively considered, and the contact trace range and stress sharing uniformity of thick glass, to generate support quality evaluation report of different thickness types of glass.

The invention relates to the technical field of astaxanthin preparation, and particularly discloses a method for increasing the yield of astaxanthin in haematococcus pluvialis, which comprises the following steps: culturing the haematococcus pluvialis to a growth plateau stage, inoculating the haematococcus pluvialis to a special improved BG-11 culture medium, and carrying out high-light high-salt induction in a columnar photobioreactor; the culture medium is designed for an induction stage and comprises limited sodium nitrate, specific content of salicylic acid, alpha-ketoglutaric acid, sodium chloride and magnesium chloride, and melatonin is not added; in the induction period, step-type increasing illumination intensity is adopted, and white light and ultraviolet light composite light quality treatment is matched. According to the method, the stress tolerance and the astaxanthin synthesis efficiency of cells are remarkably enhanced through the synergistic effect of culture medium components and a light stress procedure, the dry weight of haematococcus pluvialis is 1.69 g L <-1 >, the astaxanthin concentration of haematococcus pluvialis liquid is 105.6 mg L <-1 >, the astaxanthin content of haematococcus pluvialis powder is 6.25% (w / w) within 10 days, the final astaxanthin yield reaches 11.70 mg L <-1 > d <-1 >, and the synchronous great improvement of the astaxanthin yield and the production efficiency is realized.

This invention discloses the application of the TOE1 protein and its encoding gene in improving the tolerance of tomatoes to strong light stress or in breeding for strong light tolerance, belonging to the field of biotechnology. This invention is the first to discover that the TOE1 protein and its encoding gene play a crucial role in regulating the strong light adaptation of tomatoes. By inactivating the TOE1 protein or knocking out its encoding gene, the anthocyanin accumulation response in tomatoes under strong light stress can be reduced, thus improving the tomato's tolerance to strong light stress. Using genome editing technologies such as CRISPR / Cas9, targeted knockout of the TOE1 protein was used to create TOE1 mutant materials. These materials can alleviate the growth burden that may be caused by the severe photosensitivity response induced by excessive light in summer or in high-light areas of greenhouse agriculture, helping plants maintain more stable photosynthetic growth under strong light conditions, and providing core germplasm resources for breeding new tomato varieties adapted to high-light environments.

The invention relates to the field of coral reef ecological restoration, and particularly discloses a method for improving heat resistance of reef-building coral, which comprises the following steps: S1, light stress: continuously irradiating coral according to a first preset duration by using a first light source with light intensity of 200-300 [mu] mol ms and comprising visible light with wavelength range of 400-500 nm, and stopping irradiation for a second preset duration after the first preset duration is reached, and repeating the actions within a third preset duration. By adopting the technical scheme, the coral resistance can be improved with low damage and low cost.

The invention discloses a method for digitally evaluating and analyzing the light damage resistance of a single-molecule enzyme, which comprises the following steps of: immersing a reagent tube containing a pre-prepared enzyme solution in an ice-water mixture to form a heat clamping state, and starting a laser irradiation program to carry out light stress treatment; mixing the enzyme liquid subjected to light stress treatment with a DNA template and a primer to construct an enzyme-template-primer ternary compound, and diluting to a single molecule level; loading the ternary complex into a micropore array chip, adding a reaction substrate and a fluorescent molecular beacon for rolling circle amplification, and continuously collecting fluorescent signals to obtain a time-resolved fluorescent image sequence; constructing a U-Net network with a cross attention sub-layer, correcting the mask of the current frame, and updating the coordinates of the center of mass; after the fluorescence image sequence is processed, a correlation curve of time and fluorescence intensity of each micropore is generated, and a catalytic rate mean value and a rate distribution variance are calculated. According to the invention, the technical defects of low accuracy of analysis results caused by photo-thermal interference and signal drift are overcome.

The invention provides a Mn < 4 + >-Tb < 3 + > co-doped KZn (PO3) 3 luminescent material and a preparation method and application thereof, and belongs to the technical field of luminescent materials. KZn (PO3) 3 is used as a matrix, Mn < 4 + > and Tb < 3 + > are co-doped, an efficient energy transfer channel is established between the Mn < 4 + > and the Tb < 3 + >, in the ultraviolet excitation process, energy absorbed by the Mn < 4 + > can be partially transferred to the Tb < 3 + >, and therefore characteristic red light of the Mn < 4 + > and characteristic green light of the Tb < 3 + > are excited at the same time. In the stress action process, carriers released by a matrix are captured by Mn < 4 + > and Tb < 3 + > under the assistance of a piezoelectric field and compositely emit light, and composite light emitting has strain discoloration performance. Meanwhile, co-doping effectively stabilizes a luminescence center and significantly inhibits a thermal quenching effect. The material integrates multiple modes of multi-color luminescence under ultraviolet excitation, long afterglow, dynamic luminescence under stress triggering and the like, and a brand new material is provided for developing a new generation of optical sensing and multi-dimensional dynamic anti-counterfeiting technology.

The invention provides a quartz tube rod stress detection device, which belongs to the technical field of quartz tube rod detection and comprises a polarization stress meter main body, a quartz tube rod supporting part, an eyepiece assembly, a polarizer, an adjusting arm assembly and a clamping part. The polarized light stress meter main body comprises an eyepiece assembly and a polarizer, a quartz tube rod supporting part is arranged on the polarized light stress meter main body, the quartz tube rod supporting part comprises an adjusting arm assembly and a clamping part, and the adjusting arm assembly is connected to the polarized light stress meter main body. According to the invention, the adjusting arm assembly is connected to the polarization stress gauge main body, and the clamping part on the adjusting arm assembly fixes and supports the quartz tube rod to be detected, so that the quartz tube rod does not need to be frequently adjusted by hands. The lifting bracket moves on the base along the axial direction of the quartz tube rod, so that the whole quartz tube rod can be conveniently subjected to multi-point movable segmented detection, and the detection is different from segmented detection of cutting off the quartz tube rod.

The invention relates to the field of plant state image recognition, in particular to an illumination control method and system based on a plant state, and the method comprises the steps: carrying out the periodic shooting of a target plant, and obtaining the images of the target plant at multiple angles in each period; for each period, using the trained convolutional neural network to extract a leaf area from each frame of image, matching and numbering the leaf areas belonging to the same leaf in continuous frames of images, and retaining an optimal leaf area corresponding to each leaf; and for each leaf, calculating a state evaluation value based on all optimal leaf areas reserved in a preset period, when the state evaluation value is greater than a preset threshold value, judging that the leaf is burnt, and controlling the illumination intensity to be adjusted to safe light intensity. Two key signals of color abnormity and surface structure deterioration possibly caused by light stress of the leaves can be captured at the same time, and the method has higher detection sensitivity and comprehensive judgment for early weak burn signs.

The application discloses application of a ZmSht1 gene in improving corn resistance to high-temperature and high-light double stress, and discloses a nucleotide sequence of the ZmSht1 gene, which is shown in a sequence table SEQ ID NO:1, and an amino acid sequence of an expression protein of the ZmSht1 gene, which is shown in a sequence table SEQ ID NO:2. The ZmSht1 gene provided by the application can improve the resistance of corn to high temperature and high light, and has a higher net photosynthetic rate under high-temperature and high-light conditions in summer, and is of great significance for corn stress resistance and high-quality breeding.

The present application relates to the technical field of biological experiment apparatus, disclose a kind of multifunctional mouse stress model establishment device, comprising: box frame and feeding mechanism, restraint mechanism, illumination mechanism, temperature control mechanism, acupuncture mechanism and control center;Box frame is by mutually perpendicular connection crossbeam, stringer and vertical beam, horizontal placement base plate is connected between vertical beam, the periphery of base plate is provided with inner layer breathable baffle, and inner layer breathable baffle constitutes mouse feeding space with base plate;Mouse is restrained by restraint mechanism and carries out restraint stress experiment, mouse is carried out by illumination mechanism and carries out high light stress experiment, mouse is carried out by temperature control mechanism and carries out cold and hot stress experiment, mouse is carried out by acupuncture mechanism and carries out foot bottom acupuncture and electric shock stress experiment.The device of the present application integrates the function of a variety of stress stimulation experiments, while realizing the components or mechanisms of each stress function can be coordinated with each other, can also be independent, facilitate simultaneously experiment one or multiple stress stimulation, wide application range.

The invention relates to the technical field of plant growth regulation, in particular to application of rhodopseudomonas palustris in promoting photosynthesis of tomato leaves under weak light. When the rhodopseudomonas palustris liquid provided by the invention is sprayed on the tomato leaf surfaces, photosynthesis can be promoted, the total chlorophyll content of the tomato leaves can be obviously improved, the aging of the leaves can be delayed, and the rhodopseudomonas palustris liquid has an application value in improving the resistance of tomatoes to weak light stress.

The utility model discloses an experimental mouse feeding cage convenient to observe, through the movable track of being equipped with on cage body upper and lower, built -in movable covering plate and telescopic covering plate, with the aid of the sliding rotation of rotary ring in movable groove, cooperation ball anti -friction design, realize flexible regulation, movable covering plate can slide rotation, telescopic covering plate telescopic, both end magnetic attraction structure convenient and fast splicing separation, can adjust the range of shielding according to need, open completely or partially shield, avoid strong light stress, be convenient for multi -angle observation, can control the illumination in cage simultaneously, reduce outside interference, guarantee experimental data accurate, cylindrical cage body is free of edge, reduce mouse collision damage, and have no observation dead angle.

The invention relates to the technical field of microalgae biology, in particular to a high-biomass and high-photosynthetic-efficiency chlorella pyrenoidosa mutant strain J-1 which is obtained by mutagenizing wild type chlorella pyrenoidosa through an atmospheric pressure room temperature plasma (ARTP) mutagenesis technology and combining an efficient screening strategy. When the strain is cultured for 4 days under a standard autotrophic condition, the biomass reaches 1.23 mg / mL, the OD750 reaches 6.27, and when the strain is cultured for 5 days under a high light stress condition, the OD750 reaches 16.70; according to the invention, the growth performance is obviously enhanced: the biomass of J-1 is higher than that of WT under autotrophic, mixotrophic or heterotrophic conditions, and the biomass of a J-1 mutant strain reaches 1.23 mg / mL and is increased by 53.8% compared with that of a wild type (0.80 mg / mL) after the J-1 mutant strain is cultured for 4 days under a standard autotrophic condition (50 [mu] mol * m <-2 > * s <-1 >); the OD750 reaches 6.27 and is higher than 5.75 of a wild type, the OD750 reaches 2.18 mg / mL (WT is 1.75 mg / mL) on the fourth day of mixotrophic culture, the OD750 reaches 1.39 mg / mL (WT is 1.05 mg / mL) on the fourth day of heterotrophic culture, and it is indicated that biomass can be efficiently accumulated in different nutritional modes.