94 results about "Respiratory pathogen" patented technology

The invention is based on a micro-fluidic chip technology and performs rapid detection on seven respiratory tract pathogenic bacteria in combination with a nucleic acid isothermal amplification technology, and belongs to the field of safe and rapid detection of respiratory tract pathogenic bacteria. According to the technology, a sample subjected to rapid treatment is mixed with a reagent in a kit. The mixed liquid is added into a micro-fluidic chip. A primer pre-loaded into the micro-fluidic chip is subjected to a nucleic acid isothermal amplification reaction with a possible microbial gene template in the mixed liquid at certain single temperature. A fluorescent dye in the reaction system is combined with an amplification product to generate a fluorescent signal. Whether the seven pathogens exist in the respiratory tract or not is determined through instrument reading. According to the method, the need for the sample quantity is reduced through the micro-fluidic chip technology, the detection throughput is increased, and seven pathogenic bacteria can be detected at the same time. By adopting the nucleic acid isothermal amplification technology, the detection specificity and sensitivity are improved; the operation is simple, and the reaction time is short. A simple and rapid respiratory tract pathogenic bacteria detection technology is provided for clinical use.

The present invention relates to compositions and methods for the treatment of immunodeficiency (e.g., primary immunodeficiency disease). In particular, the invention provides human plasma immunoglobulin compositions containing select antibody titers specific for a plurality of respiratory pathogens, methods of identifying human donors and donor samples for use in the compositions, methods of manufacturing the compositions, and methods of utilizing the compositions (e.g., for prophylactic administration and / or therapeutic treatment (e.g., passive immunization (e.g., immune-prophylaxis))).

The invention discloses a method for detecting 29 respiratory pathogens by using a Taqman low-density microfluidic chip (TAC) technology. According to the method, the TAC detection technology is established for the 29 respiratory pathogens, the advantages of MGB probe detecting are fully used, the defects of previous multiple detection of the respiratory pathogens are overcome, 29-respiratory-pathogen multi-target detection of the respiratory pathogens can be achieved on a sample in two hours, and the good sensitivity and the good specificity are achieved. According to the method for detectingthe 29 respiratory pathogens by using the TAC technology, a quick, accurate and high-throughput technological mean is provided for monitoring and outbreak investigation of respiratory diseases in China.

A determination of the nucleotide sequence for the genome of Equine rhinovirus 1 (ERhV1), a horse-respiratory pathogen of heretofor uncertain taxonomic status, reveals a structure and predicted amino acid sequence that are more similar to those of foot-and-mouth disease viruses, the only members of the aphthovirus genus, than of other picornaviruses. These insights inform an understanding of ERhV1 virion proteins and virus-like particles, as well as the design of probes, primers, antigens, vectors, diagnostics and tests of relevance to ERhV1.

Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

The present invention relates to compositions and methods for the treatment of immunodeficiency (e.g., primary immunodeficiency disease). In particular, the invention provides human plasma immunoglobulin compositions containing select antibody titers specific for a plurality of respiratory pathogens, methods of identifying human donors and donor samples for use in the compositions, methods of manufacturing the compositions, and methods of utilizing the compositions (e.g., for prophylactic administration and / or therapeutic treatment (e.g., passive immunization (e.g., immune-prophylaxis))).

The invention relates to a composition for detecting 23 respiratory pathogens. The composition comprises a primer composition consisting of the sequences shown by SEQ ID NO. 1-SEQ ID NO. 48 and a probe composition consisting of the sequences shown by SEQ ID NO. 49-SEQ ID NO. 72. The invention also relates to a kit for detecting 23 respiratory pathogens and a detection method of the kit. The kit comprises a first reaction solution, a second reaction solution, a first hybridization solution and a second hybridization solution containing the primers and probes respectively; the kit also comprises a mixed enzyme, a streptavidin-phycoerythrin solution, a negative reference, a positive reference and an interior label. The kit and the detection method provided by the invention integrate the functions of RT-PCR, full-automatic liquid-phase chip hybridization detection, data analysis and the like, and also have the advantages of high throughput, multiple detection target spots, high degree of automation, easiness in operation, short time consumption among the inventions of the same kind of liquid-phase chip technologies, etc.; moreover, the kit and the detection method provide important information for addressing the respiratory tract infection epidemic in time, and are of great significance to the prevention and control of respiratory pathogens.