143 results about "Respiratory pathogen" patented technology

The invention provides a nucleic acid composition for respiratory tract pathogen detection and application thereof, and relates to the technical field of biology. The invention provides a nucleic acid detection composition which has high specificity and can be used for detecting various respiratory pathogens. The composition can be used for detecting influenza B virus, influenza A virus H1N1, influenza A virus H3N2, novel coronavirus N gene, novel coronavirus O gene, respiratory syncytial virus, I-type parainfluenza virus, II-type parainfluenza virus, III-type parainfluenza virus, IV-type parainfluenza virus, rhinovirus, adenovirus, haemophilus influenzae, and the like. The kit has 15 pathogens including streptococcus pneumoniae and staphylococcus aureus, the lower detection limit can be as low as 20 copies / reaction, and the kit plays an important role in rapid detection of respiratory tract pathogens.

The invention discloses a primer group and a kit for simultaneously detecting nucleic acids of various respiratory pathogens based on twenty-six PCR (Polymerase Chain Reaction). The kit comprises primer pool freeze-dried powder of influenza A virus (FluA), influenza B virus (FluB), respiratory syncytial virus (RSV), human parainfluenza virus (hPIV), mycoplasma pneumoniae (MP), novel coronavirus (SARS-CoV-2), human respiratory adenovirus (HADV), human respiratory nose / enterovirus (HRV / HEV), bordetella pertussis (BP), human coronavirus (HCoV), human bocavirus (HBoV) and chlamydia pneumoniae (CP), PCR (polymerase chain reaction) freeze-dried powder and a buffer solution. According to the kit, primer system optimization is carried out on various pathogens, brand new primers and probe pools are designed, clinical sensitivity and specificity can be achieved, and meanwhile, the kit is matched with a fully-closed full-automatic detection card box integrating sample treatment and amplification and is suitable for rapid detection of respiratory tract pathogens.

The invention relates to the technical field of biomedicine, in particular to a primer probe combination for broad-spectrum screening of common respiratory pathogens and application of the primer probe combination. Through screening and optimization, the respiratory pathogen nucleic acid detection primer probe combination for broad-spectrum screening of common acute respiratory infection and community-acquired pneumonia is obtained; the primer probe combination covers 18 respiratory tract pathogens, has the advantages of high accuracy, good specificity, strong anti-interference capability, good inclusiveness, high sensitivity, hyperjoint detection and the like, can realize parallel detection of 20 targets in cooperation with totally-closed single-tube PCR amplification, greatly improves early diagnosis of respiratory tract diseases with complex pathogen structures, and has wide application prospects. The method has very important significance on prevention, control and treatment of infectious diseases.

The invention discloses primers and a kit for rapidly detecting canine respiratory tract pathogens and a preparation method, and belongs to the technical field of biology. The kit comprises five groups of specific primers for rapidly detecting the canine respiratory pathogens, can rapidly and accurately detect the five respiratory pathogens of canine parainfluenza virus, canine distemper virus, canine adenovirus type II, canine crown virus and canine bordetella within 15 minutes, and is low in detection cost and low in detection environment requirement. The kit can be applied to clinical rapid diagnosis and on-site screening to timely diagnose infection, so that veterinarians can rapidly take effective treatment measures; the technical problems of short time consumption, high accuracy, low cost and simple operation cannot be simultaneously considered in the detection process in the prior art are solved.

The invention provides a kit for single-hole detection of nucleic acids of various respiratory pathogens, and belongs to the technical field of pathogen detection, the kit comprises 2-5 primer combinations and a film-forming agent; the film-forming agent is polyethylene wax; each primer combination comprises a plurality of primer pairs, and the primer pairs are used for amplifying specific respiratory tract pathogen nucleic acid. According to the invention, the reaction is divided into two systems, the two systems are separated by a film-forming agent, amplification of the two reaction systems is carried out in the same reaction tube, after multiple amplification is completed, an isolating membrane is heated and melted, two groups of amplification products are mixed, and then the amplification products are detected. The multiplex RT-PCR kit which is good in detection specificity, high in sensitivity, good in repeatability and high in detection accuracy and can be used for simultaneously detecting various pathogenic bacteria is successfully constructed.

The invention discloses a respiratory infectious disease multi-pathogen nucleic acid detection kit based on a fluorescent PCR detection technology and application of the respiratory infectious disease multi-pathogen nucleic acid detection kit. The invention provides two detection kits aiming at 20 and 6 respiratory tract detection, the two kits can specifically detect respiratory tract pathogens, the detection sensitivity is high, the whole detection system is short in time consumption and simple and convenient in use process, and detection results of 20 pathogenic microorganisms can be reported within 40 min at the shortest; the whole set of detection kit and the detection method are high in sensitivity and good in specificity; the normal-temperature storage and transportation of the reagent can be realized.

The invention discloses a nucleic acid combination product, a kit and a method for detecting various respiratory tract pathogenic bacteria. The nucleic acid combination product comprises a primer pair and a probe, wherein the sequences of the primer pair and the probe are respectively shown as SEQ ID NO: 1-SEQ ID NO: 24. The nucleic acid combination product realizes synchronous detection of various pathogens through a one-tube eight-color fluorescent PCR, freeze-drying and ultrasonic direct diffusion method technology, significantly reduces experimental operation steps, shortens detection time, reduces the complexity of result interpretation, and has strong anti-interference capability and high sensitivity.

The invention discloses a method and system for detecting respiratory tract pathogens, pathogen drug-resistant genes and pathogen virulence genes, and relates to the technical field of gene detection.The method comprises the steps that a respiratory tract sample of a patient is collected, DNA and RNA are extracted, and total nucleic acid is obtained; carrying out first-round PCR amplification on the total nucleic acid, purifying a first-round PCR product, carrying out second-round PCR amplification, and sorting the product to obtain a library building product; performing high-throughput sequencing on the library building product to obtain sequencing data, and detecting pathogens, drug-resistant genes and virulence genes; according to the method and system for detecting the respiratory tract pathogens, the pathogen drug-resistant genes and the pathogen virulence genes, by optimizing library preparation and direct library building of nucleic acid, the process is simplified, and efficiency and accuracy are improved; the balance reagent design guarantees library quality; the kit adapts to pathogen detection requirements in Yunnan and the like, provides efficient diagnosis, shortens detection time, improves treatment effect, regularly updates pathogen information, and keeps technical frontier.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The invention relates to the technical field of molecular diagnosis, in particular to a multiplex nucleic acid detection primer probe combination and application thereof. A corresponding primer probe combination is designed for researching 10 common respiratory tract pathogens such as new coronavirus, influenza A virus, influenza B virus, adenovirus, rhinovirus, respiratory syncytial virus and human parainfluenza virus types 1, 2, 3 and 4, a respiratory tract infection multiple pathogen detection system is established, a multiple RT-qPCR system is optimized, and the kit is used for detecting the respiratory tract infection multiple pathogens. The optimized system is applied to a clinical throat swab sample for verification, and the multiple pathogen nucleic acid detection kit is researched and developed and used for detecting viral respiratory tract infection. According to the invention, multiple pathogens are detected by adopting a multiple RT-qPCR method, so that the detection time can be shortened, the detection sensitivity and specificity are improved, the detection cost is reduced, and the kit has important clinical significance on rapid diagnosis of respiratory tract multiple pathogen infection.

The invention discloses a nucleic acid combination product for detecting various respiratory pathogens, a kit containing the nucleic acid combination product and a method. The nucleic acid combination product comprises a primer pair and a probe, wherein the nucleotide sequences of the primer pair and the probe are respectively shown as SEQ ID NO: 1-21. According to the nucleic acid combination product, on the technical basis of Taqman fluorescent quantification, seven targets can be detected by one tube, so that the time and energy of operators are greatly shortened, and the nucleic acid combination product is convenient and rapid, is not easy to make mistakes, and is simpler and easier to understand in result interpretation.

The invention discloses a liquid-phase hybridization probe pool for joint detection of multiple pathogens and drug-resistant genes. The liquid-phase hybridization probe pool is designed according to target areas of target pathogens and drug-resistant genes; the probe is a probe with unequal length of 30 to 100 nt; comprising a target specific sequence complementarily paired with a target region and a conjugate sequence complementarily paired between adjacent probes; the 5'terminal and the 3 'terminal of the probe are respectively modified with a conjugate sequence A and a conjugate sequence B, so that the adjacent probes can be specifically combined through terminal pairing; the conjugate sequence A is as shown in SEQ ID No. 601, and the conjugate sequence B is as shown in SEQ ID No. 602. According to the method, the constructed library and the specific probe pool are hybridized, so that the capture time is shortened to 30 minutes, and the method is obviously superior to a traditional method. Through optimization, the operation time and complexity are reduced, personal errors are reduced, meanwhile, high sensitivity and specificity are kept, target nucleic acid in a complex sample can be efficiently captured, a high-quality template is provided for subsequent sequencing, and combined detection of multiple respiratory pathogens and drug-resistant genes is supported.

The invention relates to the technical field of molecular biological detection, and particularly provides a reagent for respiratory tract infection pathogen identification and drug resistance and virulence gene detection. Each primer in the plurality of primers comprises a first sequence as shown in SEQ ID NO: 1-165 and an optional second sequence for distinguishing sample sources. Target spots detected by the super-multiplex primer combination comprehensively cover pathogen directories (totally 353 types) of latest respiratory tract infection diagnosis guidelines at home and abroad, low-cost, high-sensitivity, high-specificity and high-flux detection is achieved, and clinical medication can be effectively and comprehensively guided.

The present invention relates to a respiratory pathogenic microorganism and drug resistance gene nucleic acid testing kit (gene chip method), comprising a kit body. The kit body comprises a PCR amplification reaction reagent, PCR amplification reaction consumables, gene chip hybridization reaction reagents, gene chip hybridization reaction consumables, and a specific gene probe. The gene chip hybridization reaction reagents include a hybridization solution and a 1000X cleaning solution; the gene chip hybridization reaction consumables include a gene chip and a coverslip; the PCR amplification reaction reagent is a PCR buffer solution; and the PCR amplification reaction consumables are PCR tubes. On the basis of gene chip technology in combination with novel multiplex PCR amplification technology, the present invention provides a novel respiratory pathogenic bacterium and drug resistance gene testing technology which has high testing throughput and sensitivity and good identification accuracy, is simple and easy to operate and low-cost, and is widely used as a conventional testing means; and testing results are used for guiding treatment plans and medications for patients suffering from respiratory diseases and even acute and severe patients.

The present invention discloses a mask-type respiratory pathogen sampling device, comprising a mask, an air supply pipe, a collecting pipe, a pipe cover, an air dispersion coil, an exhaust pipe and a cold storage box, wherein the mask is provided with a one-way valve air inlet and an air outlet; the collecting pipe is detachably provided with a pipe cover, and an air dispersion coil and an exhaust pipe are inserted on the pipe cover, and the air dispersion coil runs through the bottom of the collecting pipe; one end of the air supply pipe is connected to the air outlet of the mask, and the other end is connected to the pipe mouth of the air dispersion coil; the collecting pipe is filled with pathogen collection and preservation liquid; the collecting pipe can be adaptively placed in the cold storage box. The innovative point of the present invention is that the mask intercepts the gas exhaled by the sampled person, introduces it into the air dispersion coil immersed in the sampling preservation liquid, and then exhausts the gas through the micropores on the spiral air dispersion coil, dissolving the pathogens carried in the bubbles in the pathogen collection and preservation liquid, so as to achieve the purpose of efficiently collecting respiratory pathogens.

The invention relates to the field of public health, in particular to an intelligent identification and real-time early warning system for unknown pathogens in respiratory tract samples of fever outpatient service, which comprises a data quality control processing module for performing quality control and standardized preprocessing on metagenome original sequencing data to obtain pure microorganism reading length; the known pathogen identification and annotation module is used for identifying known pathogens, classifying non-host reads, constructing a classification database according to a classification result, and annotating reads with low classification confidence or unclassified reads in the classification result to obtain an annotation table; the recognition model training module is used for constructing a positive and negative sample data set and training a recognition model; and the unknown pathogen identification module is used for identifying potential unknown pathogens in the metagenome of the pure microorganism read length by adopting the trained identification model according to the classification database and the annotation list. According to the invention, identification, classification and dynamic monitoring of known and unknown respiratory pathogens can be realized.

The invention discloses a primer composition for respiratory tract pathogen detection, a related product and application, and belongs to the field of pathogen detection. The primer composition comprises a primer pair aiming at influenza A virus, a primer pair aiming at influenza B virus, a primer pair aiming at bordetella pertussis, a primer pair aiming at adenovirus, a primer pair aiming at respiratory syncytial virus and a primer pair aiming at mycoplasma pneumoniae. The primer composition and related products can be used for simultaneously detecting influenza A viruses (seasonal H1N1, novel influenza A H1N1 viruses (2009), H3N2, H5N1 and H7N9), influenza B viruses (Victoria series and Yamagata series), adenoviruses (group B, group C and group E), respiratory syncytial viruses (subtype A and subtype B), mycoplasma pneumoniae and bordetella pertussis, so that pathogenic pathogens are quickly determined, and the primer composition and related products have important significance on clinical detection.

The invention relates to the field of pathogen detection, in particular to a respiratory tract pathogen nucleic acid detection kit which comprises an adenovirus upstream primer, a downstream primer and an adenovirus probe. The kit comprises a rhinovirus upstream primer, a rhinovirus downstream primer, a rhinovirus probe, a parainfluenza virus type I upstream primer, a parainfluenza virus type I downstream primer and a parainfluenza virus type I probe; a parainfluenza virus type II upstream primer, a parainfluenza virus type II downstream primer and a parainfluenza virus type II probe; the kit comprises a parainfluenza virus type III upstream primer, a parainfluenza virus type III downstream primer and a parainfluenza virus type III probe. The kit provided by the invention can quickly and accurately detect and distinguish the respiratory pathogens, and the actual detection time is only 24-35 minutes, which is far shorter than that of the kit in the prior art.

The invention relates to the technical field of biology, in particular to a primer probe combination product for respiratory tract infection detection. A plurality of specific primers and self-quenching probes are integrated in a single reaction system; simultaneous, rapid and rapid detection on 15 respiratory pathogens (including influenza A virus, influenza B virus, respiratory syncytial virus, severe acute respiratory syndrome coronavirus 2, mycoplasma pneumoniae, parainfluenza virus, rhinovirus, enterovirus, coronavirus, adenovirus, metapneumovirus, bordetella pertussis, bocavirus, chlamydia pneumoniae and chlamydia psittaci) is realized. The detection is accurate.

The invention discloses a primer probe combination and a kit for detecting nucleic acids of 14 respiratory pathogens and application, and belongs to the technical field of pathogen detection. The technical problem to be solved is that the detection of respiratory pathogens at the present stage is not only limited by a complex operation process, but also is difficult to meet the clinical rapid diagnosis requirement. Meanwhile, due to the inherent technical bottleneck, the problems of insufficient sensitivity, high false negative rate and the like exist. According to the technical scheme, the primer probe combination for simultaneously detecting 14 respiratory pathogens is provided, and based on the Real-time PCR technology, rapid, specific and accurate detection of the 14 respiratory pathogens is achieved.

The application discloses a set of molecular targets, primer compositions, methods and applications for identifying respiratory pathogens, the respiratory pathogens are novel coronavirus, influenza A virus and respiratory syncytial virus, and nucleotide sequences of the molecular targets are shown as SEQ ID NO:1-3. The application provides molecular targets, primers and probes for detecting three respiratory pathogens, and establishes a triple real-time RT-RAA detection technology, which can realize rapid identification and differentiation of three respiratory infectious disease pathogens with similar clinical symptoms within 30 minutes, reduces the operation threshold of the existing detection technology, realizes the target of rapidly, simply and accurately identifying and differentiating the three viruses, meets the needs of early clinical diagnosis of infection, provides technical support for precise medication, and has wide application value.

The present invention provides dual-target site reverse transcription fluorescent PCR primers, probes, and a kit for detecting the novel coronavirus SARS-CoV-2. By performing nucleic acid sequence alignment on the entire genome of the novel coronavirus SARS-CoV-2, three specific nucleic acid sequences W1, W2, and W3 in the viral N gene are found, and the sequences are shown as SEQ ID NOs. 1-3, respectively. Multiple pairs of primers and probes are designed for the three target sites, respectively. It is found that the dual-target site reverse transcription fluorescent PCR using a primer-probe combination designed for the W1 and W2 targets (sequences shown as SEQ ID NOs. 4-6 and SEQ ID NOs. 7-9, respectively) can specifically and sensitively detect the novel coronavirus SARS-CoV-2, with a detection sensitivity of less than 10 copies. There is no non-specific amplification of 22 common clinical respiratory pathogens, which can effectively avoid false negative results caused by single target site mutations and has good specimen detection capabilities.

The application discloses a nucleic acid combination product, a kit and a method for detecting multiple respiratory tract pathogenic bacteria. The nucleic acid combination product comprises a primer pair and a probe, wherein the sequences of the primer pair are respectively shown as SEQ ID NO:1-SEQ ID NO:24. The nucleic acid combination product realizes the synchronous detection of multiple pathogenic bacteria through one-tube eight-color fluorescence PCR, freeze-drying and ultrasonic direct extension technology, significantly reduces the experimental operation steps, shortens the detection time, reduces the complexity of result interpretation, and has strong anti-interference ability and high sensitivity.

The invention relates to the technical field of molecular biological detection, in particular to a multiplex digital PCR (polymerase chain reaction) kit for detecting various respiratory pathogens, which comprises a primer pair aiming at various targets and a unique fluorescence proportion coding probe group, each group of probes comprises probes marked with two fluorophores with different proportions, and high-throughput identification of multiple targets in a single reaction is realized through fluorescence proportion combination. The kit is designed by adopting a gradient reaction system, primer pairs are grouped according to a non-interference principle, and concentration is adjusted according to target expected abundance differentiation, so that amplification bias is effectively inhibited, and quantitative accuracy in a wide dynamic range is ensured. The invention also provides a complete detection scheme containing the internal standard system, and is suitable for rapid, accurate and multiple absolute quantitative detection of respiratory tract infection pathogens.

The application discloses a swab collecting and transporting device for respiratory pathogen detection and relates to the technical field of medical devices.The device comprises a tube body and a tube cover, and the tube body is composed of an outer tube body, an inner container and a vacuum heat preservation layer; the inner container is provided with a U-shaped breaking gap at the container mouth, the gap is provided with a wedge-shaped cutting edge, and a limiting block provided with anti-skid serrations is arranged below; the container mouth is provided with a sealing flow blocking block and a rubber plug body, the plug body is provided with a medical hydrophilic liquid absorbing composite buffer layer at the bottom; and the outer tube body is provided with a real-time temperature display unit, which can adopt reversible heat-sensitive color-changing strips or waterproof miniature digital display modules.The wedge-shaped cutting edge and the limiting structure are used to realize accurate and labor-saving breaking of the swab, so that sample spatter and pollution are avoided; multi-stage sealing and liquid absorbing buffer layer double anti-overflowing can quickly absorb and inactivate leaked samples; vacuum heat preservation is combined with real-time temperature monitoring to guarantee the cold chain storage environment of the samples.The whole operation is convenient and safe, the device is suitable for clinical sampling processes, and the reliability of respiratory pathogen sample collection, preservation and transportation can be effectively improved.

The present invention relates to the field of detection of multiple respiratory pathogens in dogs, and specifically to a nucleic acid composition and a kit and method for detecting multiple respiratory pathogens in dogs. The kit uses RPA amplification reagents to directly and effectively amplify clinical samples, and the amplified products are targetedly cut by CRISPR reagents to generate fluorescent signals, and multiple respiratory pathogens in dogs are qualitatively or quantitatively detected based on the fluorescent signals. The kit and the detection method have high sensitivity, strong specificity, and are simple to operate. The detection personnel only need to add samples to detect, which reduces errors and deviations caused by the liquid preparation process, and does not require professional technicians.

Provided are methods for inducing immune responses against pathogens in a mammal. The methods comprise administering a single cycle adenovirus (SC-Ad) comprising a nucleic acid sequence encoding an immunogen of said pathogen to said mammal. The SC-Ad is administered by inhalation and infects a cell of said mammal, thereby causing expression of said immunogen leading to the induction of said immune response. New infectious virions are not produced. The methods may be of particular utility in vaccination for respiratory pathogens. Corresponding medical uses of the SC-Ads are also provided.

The invention relates to the field of biological medicines, and discloses application of a GOSR1 gene in prevention and / or treatment of respiratory pathogens. The GOSR1 gene is specifically knocked down, so that the gene and protein expression level of the pathogen can be reduced, and the proliferation of the respiratory pathogen can be remarkably inhibited. In addition, the invention also discloses that overexpression of the GOSR1 gene can activate proliferation of pathogens in cells infected by respiratory pathogens. Based on the regulation effect of the GOSR1 gene on respiratory tract pathogen proliferation, a brand new strategy is provided for treatment of respiratory tract pathogen infection, and the application is particularly suitable for intervention of influenza A virus infection.

The invention is suitable for the technical field of cell separation and culture, and provides a pig nasal mucosa epithelial cell separation and culture method. A mixed enzyme overnight digestion method is adopted, mucous membrane tissue mucus is effectively removed, and more complete digestion is achieved. The pig nasal mucosa epithelial cells with relatively high purity are obtained through a differential attachment technology. And the culture medium is replaced by a complete culture medium containing EGF and vitamin A during first liquid replacement, so that a stable and excellent in-vitro growth environment is provided for epithelial cells. The method not only can rapidly and accurately separate the primary cells, but also ensures the stability and high quality of the culture state of the epithelial cells. According to the method, the blank of porcine nasal mucosa primary cell separation research is filled up, the primary porcine nasal mucosa epithelial cell in-vitro model which is simple and easy to implement and high in repeatability is constructed, and the model can simulate physiological and morphological characteristics of porcine nasal mucosa and can be used for preparing the porcine nasal mucosa epithelial cell in-vitro model. And a reliable in-vitro experimental basis is provided for researches on respiratory tract pathogen infection mechanisms, respiratory system disease drug screening, gene therapy and the like.

The application provides a quality control for detecting respiratory pathogen nucleic acid, which comprises any one or more of the following respiratory pathogens: coronavirus, influenza virus, adenovirus, Mycoplasma pneumoniae, Streptococcus pneumoniae, rhinovirus or / and Legionella pneumophila. The full-length genome sequence of the coronavirus is divided into 6 target fragments, each with a length of 4000-5500 bp. The quality control of the application covers all detection target sequences (or target points) of the coronavirus, has a wide coverage, comprehensively detects target points, and does not have the phenomenon of missed detection. The quality control of the application contains main respiratory pathogen infections, has a wide range, and uses real virus samples and lentivirus samples as raw materials, and has more accurate detection target points. The preparation of the coronavirus quality control of the application integrates the target gene sequence into the host genome through the lentivirus vector, knocks out the self-replication gene in the preparation process, and has a "self-inactivation" ability, so that the recombinant lentivirus cannot replicate in the target cells and infect other cells.