95 results about "Respiratory pathogen" patented technology

A biosafe apparatus is disclosed for assay and diagnosis of respiratory pathogens comprising a nasal sampling device, a single entry, disposable microfluidic cartridge for target nucleic acid amplification, and an instrument with on-board assay control platform and target detection means.

The present invention is directed to methods, compositions and kits for multiplex detection of pathogens, such as respiratory pathogens.

The invention discloses a Multiplex PCR detection kit for nucleic acids of twelve respiratory viruses. The kit includes primers for amplifying the specific gene loci of the following twelve respiratory viruses: influenza A virus (Inf A), influenza A H1N1 (2009), influenza A H3N2, human parainfluenza virus (HPIV1), human parainfluenza virus (HPIV2), human parainfluenza virus (HPIV3), human parainfluenza virus (HPIV4), human metapneumovirus (hMPV), respiratory adenovirus (AdV), respiratory syncytial virus (RSV), bocavirus (BoV) and coronavirus (CoV). The kit allows multiplex detection, has high sensitivity and is convenient and quick to use. Specific primer sequences ensure reliability of detection results. A detection method is simple in operation, save time and labor intensity, has high detection throughput, is low in cost of reagent and consumable, and can directly detect the nucleic acids extracted from a respiratory pathogen sample, is low in requirement on detection platform and operation technology, and can be widely promoted in common detection.

The invention is based on a micro-fluidic chip technology and performs rapid detection on seven respiratory tract pathogenic bacteria in combination with a nucleic acid isothermal amplification technology, and belongs to the field of safe and rapid detection of respiratory tract pathogenic bacteria. According to the technology, a sample subjected to rapid treatment is mixed with a reagent in a kit. The mixed liquid is added into a micro-fluidic chip. A primer pre-loaded into the micro-fluidic chip is subjected to a nucleic acid isothermal amplification reaction with a possible microbial gene template in the mixed liquid at certain single temperature. A fluorescent dye in the reaction system is combined with an amplification product to generate a fluorescent signal. Whether the seven pathogens exist in the respiratory tract or not is determined through instrument reading. According to the method, the need for the sample quantity is reduced through the micro-fluidic chip technology, the detection throughput is increased, and seven pathogenic bacteria can be detected at the same time. By adopting the nucleic acid isothermal amplification technology, the detection specificity and sensitivity are improved; the operation is simple, and the reaction time is short. A simple and rapid respiratory tract pathogenic bacteria detection technology is provided for clinical use.

The present invention relates to compositions and methods for the treatment of immunodeficiency (e.g., primary immunodeficiency disease). In particular, the invention provides human plasma immunoglobulin compositions containing select antibody titers specific for a plurality of respiratory pathogens, methods of identifying human donors and donor samples for use in the compositions, methods of manufacturing the compositions, and methods of utilizing the compositions (e.g., for prophylactic administration and / or therapeutic treatment (e.g., passive immunization (e.g., immune-prophylaxis))).

The invention is about the PCR detecting method of a respiratory tract pathogen and the reagent box. The character is to design the vector and the probe according to the highly special base groups of the pathogen by analyzing the nucleic acid sequence of the pathogen. So next to amplify the PCR or the RT-PCR in the fluorescent PCR instrument.

The invention discloses a method for detecting 29 respiratory pathogens by using a Taqman low-density microfluidic chip (TAC) technology. According to the method, the TAC detection technology is established for the 29 respiratory pathogens, the advantages of MGB probe detecting are fully used, the defects of previous multiple detection of the respiratory pathogens are overcome, 29-respiratory-pathogen multi-target detection of the respiratory pathogens can be achieved on a sample in two hours, and the good sensitivity and the good specificity are achieved. According to the method for detectingthe 29 respiratory pathogens by using the TAC technology, a quick, accurate and high-throughput technological mean is provided for monitoring and outbreak investigation of respiratory diseases in China.

A determination of the nucleotide sequence for the genome of Equine rhinovirus 1 (ERhV1), a horse-respiratory pathogen of heretofor uncertain taxonomic status, reveals a structure and predicted amino acid sequence that are more similar to those of foot-and-mouth disease viruses, the only members of the aphthovirus genus, than of other picornaviruses. These insights inform an understanding of ERhV1 virion proteins and virus-like particles, as well as the design of probes, primers, antigens, vectors, diagnostics and tests of relevance to ERhV1.

The invention provides dual-target site inverse-transcription fluorescent PCR primers, probes and kits for detection of 2019 new coronavirus (2019-nCoV). Nucleotide sequence alignment is performed onthe whole genome of the 2019-nCoV, 3 specific nucleotide sequences W1, W2 and W3 are found in N genes of the virus, and are shown in SEQ ID No. 1-3, multiple pairs of primer probes are designed separately for three target sites, and it is found that when a primer probe combination which is designed for the target sites of W1 and W2 and has sequences shown in SEQ ID No. 4-6 and SEQ ID No. 7-9 is applied to double-target site inverse-transcription fluorescent PCR, 2019-nCoV can be detected specifically and sensitively with detection sensitivity of within 10 copies; and no non-specific amplification of 22 clinically-common respiratory pathogens are caused, false-negative results caused by single target spot mutation can be avoided effectively, and good detection ability of specimens is achieved.

The invention discloses a kit for simultaneously detecting nucleic acids of seven respiratory pathogens and application of the kit. The kit is based on a double amplification technology (RNA isothermal amplification and multi-biotin signal amplification), and can detect H1N1 / H3N2 type influenza A viruses, influenza B viruses, respiratory syncytial viruses, 1 / 2 / 3 type human parainfluenza viruses, B / E type adenoviruses, mycoplasma pneumoniae and chlamydia pneumoniae. The kit of the invention does not need RNA extraction, and is not prone to pollution, high in sensitivity and good in specificityin the process of detection, and can be widely applied to detection of the nucleic acids of the above seven respiratory pathogens.

Described are kits and methods useful for detection of respiratory pathogens (influenza A (including subtyping capability for H1, H3, H5 and H7 subtypes) influenza B, parainfluenza (type 2), respiratory syncytial virus, and adenovirus) in a sample. Genomic sequence information from the respiratory pathogens was analyzed to identify signature sequences, e.g., polynucleotide sequences useful for confirming the presence or absence of a pathogen in a sample. Primer and probe sets were designed and optimized for use in a PCR based, multiplexed Luminex assay to successfully identify the presence or absence of pathogens in a sample.

The invention discloses a PCR (polymerase chain reaction) primer group, a probe set and a kit for detecting multiple respiratory pathogens. Sequences of PCR primers are represented as SEQ ID NO.1-SEQ ID NO.12; probes comprise at least two of six molecular beacon probes for respiratory pathogens, a fluorescence group and a quenching group are formed at two ends of each molecular beacon probe respectively, and sequences are represented as SEQ ID NO.13-SEQ ID NO.18. The invention further discloses a PCR reaction liquid containing the PCR primers and the probes as well as a kit containing the PCR reaction liquid for at least one person. With the adoption of the PCR primer group, the probe set and the kit, the six respiratory pathogens can be rapidly and accurately detected in a typing manner.

The present invention relates to compositions and methods for the treatment of immunodeficiency (e.g., primary immunodeficiency disease). In particular, the invention provides human plasma immunoglobulin compositions containing select antibody titers specific for a plurality of respiratory pathogens, methods of identifying human donors and donor samples for use in the compositions, methods of manufacturing the compositions, and methods of utilizing the compositions (e.g., for prophylactic administration and / or therapeutic treatment (e.g., passive immunization (e.g., immune-prophylaxis))).

The invention discloses a multi-liquid-phase gene chip detection primer, kit and method for quickly distinguishing 5 respiratory pathogens of a mouse. The multi-liquid-phase gene chip detection primer is easy to operate; a target amplified fragment is obtained through PCR (Polymerase Chain Reaction); an amplified product, fluorescence coded microspheres and streptavidin-phycoerythrin are hybridized; an MFI (Mean Fluorescence Intensity) value is read through a detector so as to distinguish different types of viruses. The method disclosed by the invention can detect a pneumonia virus, a hantaan virus, a sendai virus, a lymphocytic choriomeningitis virus and mycoplasma pulmonis of the mouse at the same time, and is high in specificity, high in sensitivity and high in repetitiveness. Compared with the conventional detection method, the method disclosed by the invention realizes simultaneous detection of various different target molecules in the same sample; the sample use amount is small; the operation is simple and quick; the detection cost can be greatly reduced.

The invention belongs to the technical field of livestock disease detection, and discloses a multi-fluorescent quantitative PCR reagent kit capable of synchronously detecting three bovine respiratorypathogens as well as a multi-fluorescent quantitative PCR detection method capable of synchronously detecting the three bovine respiratory pathogens, wherein the three bovine respiratory pathogens arerespectively mycobacterium bovis, mycoplasma bovis and Klebsiella pneumoniae. According to the multi-fluorescent quantitative PCR detection method capable of synchronously detecting the three bovinerespiratory pathogens, 3 groups of specific primers and probes are designed and synthesized on basis of a specific sequence 229bp of the mycobacterium bovis, a uvrC gene of the mycoplasma bovis and aKhe gene of the Klebsiella pneumoniae, thereby establishing the multi-fluorescent quantitative PCR detection method capable of synchronously detecting the mycobacterium bovis, the mycoplasma bovis andthe Klebsiella pneumoniae which are associated with respiratory diseases in cattle. The multi-fluorescent quantitative PCR detection method is capable of synchronously detecting the 3 bacteria; and sensitivity, repeatability and specificity tests have proven that the detection method is high in sensitivity, strong in specificity and relatively good in repeatability.

The invention relates to a multiplex real-time PCR kit and method for detecting respiratory pathogens and an application. The kit comprises a reagent for detecting influenza A viruses, influenza B viruses, human rhinoviruses and human metapneumoviruses; the reagent comprises a probe for detecting the viruses, a primer pair for specifically amplifying corresponding virus target genes, a reaction buffer solution, an enzyme mixture, a positive control and a negative control; and a detection channel of the influenza A viruses is FAM, a detection channel of the influenza B viruses is VIC, a detection channel of the human rhinoviruses is Texas Red, and a detection channel of the human metapneumoviruses is Cy5. The kit disclosed by the invention can be used for simultaneously detecting the four kinds of viruses, simplifies the operation process, shortens the detection time, improves the detection flux and reduces the detection cost while having high sensitivity and specificity, and can be universally applied to various fluorescent quantitative PCR instruments.

The invention belongs to the technical field of biomedicine and relates to an application of TFF2 protein in preparation of a medicine for treating and preventing lung / bronchial acute inflammation diseases. In the invention, the significantly different gene expression of the lung tissues of mice infected by severe flu and mild flu are analyzed and screened by a transcriptome chip, and the screened TFF2 is a protective factor; and according to in-vitro synthesis of TFF2 protein, the survival rate of the flu-infected mice can be remarkably increased by dropwise adding the TFF2 protein while the weight loss of mice is reduced. Further study shows that the TFF2 does not influence the flu virus replication but can reduce the secretion of inflammatory factors, promote the repair of the pulmonary mucosa and reduce the damage of lung tissues. Since the mechanism of other respiratory pathogens or physical and chemical factors causing respiratory tissue damage is similar to that of flu virus infection and the protection of TFF2 on flu virus infection is not specific protection but broad-spectrum protection, the TFF2 plays an important protection role in an acute inflammation damage model induced by respiratory pathogens or other factors.

The invention relates to a composition for detecting 23 respiratory pathogens. The composition comprises a primer composition consisting of the sequences shown by SEQ ID NO. 1-SEQ ID NO. 48 and a probe composition consisting of the sequences shown by SEQ ID NO. 49-SEQ ID NO. 72. The invention also relates to a kit for detecting 23 respiratory pathogens and a detection method of the kit. The kit comprises a first reaction solution, a second reaction solution, a first hybridization solution and a second hybridization solution containing the primers and probes respectively; the kit also comprises a mixed enzyme, a streptavidin-phycoerythrin solution, a negative reference, a positive reference and an interior label. The kit and the detection method provided by the invention integrate the functions of RT-PCR, full-automatic liquid-phase chip hybridization detection, data analysis and the like, and also have the advantages of high throughput, multiple detection target spots, high degree of automation, easiness in operation, short time consumption among the inventions of the same kind of liquid-phase chip technologies, etc.; moreover, the kit and the detection method provide important information for addressing the respiratory tract infection epidemic in time, and are of great significance to the prevention and control of respiratory pathogens.

The invention discloses a kit for detecting respiratory pathogens of community-acquired pneumonia, and belongs to the technical field of biomedical detection. The kit comprises a reaction solution, aprimer solution, an enzyme solution, a negative quality control substance, a positive quality control substance, a hybrid color developing solution, a hybrid membrane strip and a desiccant, wherein aprobe is attached to the hybrid membrane strip, the hybrid color developing solution comprises microspheres connected with a second coloring marker, and the color is developed after the microspheres are enriched. The detection kit involves 24 common respiratory pathogens of CAP, and has the advantages of high specificity, high sensitivity, large number of detection targets, simple and rapid operation and the like.

The invention discloses a medicine composition for treating flu and a preparation method thereof. The medicine composition is mainly prepared from glycyrrhizic acid, baicalin, pseudoephedrine, taurine and other compounds. The invention has the advantages of simple medicine composition and exact curative effect; and animal experiments indicate that the medicine composition has strong inhibiting effect on influenza A virus and influenza B virus, and has the effects of killing various respiratory tract pathogenic bacteria to different degrees. The medicine composition has obvious and long-lasting antipyretic action and obviously relieves various cold symptoms, such as rhinorrhoea, snuffle, pains, cough and the like. The medicine composition has the action of synergy, can address both the symptoms and root causes against the causes and the symptoms of cold, and can obviously shorten the course of cold. The medicine composition can be made into any acceptable excipient in pharmaceutics.

Equine rhinovirus 1 (ERhV1) is a respiratory pathogen of horses which has an uncertain taxonomic status. The nucleotide sequence of the ERhV1 genome and amino acid sequence have been substantially determined (FIG. 2). The predicted polyprotein was encoded by 6,741 nucleotides and possessed a typical picornavirus proteolytic cleavage pattern, including a leader polypeptide. The genomic structure and predicted amino acid sequence of ERhV1 were more similar to those of foot-and-mouth disease viruses (FMDV), the only members of the aphthovirus genus, than other picornaviruses. Nucleotide sequences coding for the complete polyprotein, the polymerase, and VP1 were analyzed separately. The phylogenetic trees confirmed that ERhV1 was more closely related to aphthoviruses than to other picornaviruses. Virion proteins and virus-like particles are described and probes, primers, antigens, vectors, diagnostics and tests developed.

The invention relates to the field of inspection and quarantine, and aims to provide a multi-linkage probe amplification identification kit capable of detecting a plurality of avian respiratory pathogens. The kit comprises: a pre-amplification primer mixed liquid containing pre-amplification primers with a sequence as shown in SEQ ID NO: 1-14; a probe mixed liquid containing a left probe and a right probe with sequences as shown in SEQ ID NO: 15-28; MLPA buffer solution; ligation buffer solution A; ligation buffer solution B; Ligase-65; PCR reaction mixed liquid containing a universal primer with a sequence as shown in SEQ ID NO: 29 and 30; SALSA polymerase; negative control; and positive control. The kit of the invention has the characteristics of high flux, can simultaneously detect 7 pathogens, and can automatically detect 96 samples at a time by using a fully automatic nucleic acid analyzer, and provides a high-flux detection technology for differential diagnosis and emergency diagnosis of pathogens. In addition, the kit has the advantages of good specificity and high sensitivity, and can detect at least 1 copy of target genes by increasing a RT-PCR step to enrich target genes.

The invention provides a target sequence for detecting mycoplasma pneumoniae and a detection kit. According to the target sequence, genes of the mycoplasma pneumonia are firstly sequenced and compared and are then screened to obtain multi-copy repeated-sequence genes for detecting the mycoplasma pneumonia, and the sequence of the target sequence is represented by SEQ ID NO.1; a real-time fluorescence quantitative PCR primer and a probe for detecting the target sequence are designed, and the nucleotide sequences of the real-time fluorescence quantitative PCR primer and the probe are respectively represented by SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. According to the detection kit, the sensitivity for detecting the mycoplasma pneumonia can be as low as 0.2CFU; the detection kit has no nonspecific amplification to clinically common 23 respiratory pathogens, presents good specificity and has the advantages that the detection is accurate, easy, convenient and rapid, the sensitivity is high, the specificity is strong, and the sample detection capacity is good.

The invention provides a kit for detecting nucleic acid of respiratory tract pathogens. The kit comprises a CRISPR-Cas detection system: crRNA, Cas protein and a nucleic acid probe as shown in the invention. The invention also provides a detection method of the nucleic acid of the respiratory tract pathogen, a separated nucleic acid, crRNA, a primer pair and application thereof. The kit and the detection method disclosed by the invention do not depend on large instruments, enable the results to be directly observed by naked eyes, can realize detection under mild conditions, and are more convenient in detection; the kit and the detection method provided by the invention can be used for efficiently and quickly detecting / diagnosing respiratory pathogens (such as influenza A, influenza B and novel coronavirus); the kit is high in specificity and sensitivity, and can be used for detecting and screening respiratory pathogens, such as rapidly distinguishing various respiratory pathogens including influenza A, influenza B and novel coronavirus.

The invention discloses a primer combination capable of identifying enterovirus type 71 and application of the primer combination. The primer combination disclosed by the invention is composed of 6 types of DNA (Deoxyribonucleic Acid) molecules shown as a sequence 1, a sequence 2, a sequence 3, a sequence 4, a sequence 5 and a sequence 6. The primer combination can be used for identifying or assisting to identify whether a virus to be detected is the enterovirus type 71 or not, can also be used for detecting whether a sample to be detected is infected with the enterovirus type 71 or not. A detection method provided by the invention can be used for successfully detecting the enterovirus type 71 without a non-specific reaction with other common respiratory pathogen microorganisms; the detection method is high in accuracy, high in sensitivity and high in specificity.

The invention discloses a kit and a method for gene chip detection of 16 respiratory pathogens. The kit comprises a PCR buffer solution, a reverse transcriptase, dNTPs, primers, probes, a Taq polymerase and metal cations, wherein the probes comprise hybridization probes and internal standard probes; the primers comprise 16 respiratory pathogen primers, 2 internal standard primers and 1 universal primer; the sequences of the primers are shown in SEQ ID NO:1-37; the sequences of the probes are shown in SEQ ID NO:38-55. On the basis of the PCR technology, the primers are designed for conserved domains of the 16 respiratory pathogens to amplify the target nucleotide sequence, an amplification product is subjected to hybridization and color development by means of a gene chip hybridization technology, color development conditions of hybridized spots on a gene chip are compared with a standard card finally, and a detection result is analyzed and judged.

The invention discloses a primer combination for nanopore sequencing library building of respiratory pathogens and an application of the primer combination. The primer combination for library buildingis utilized to carry out the nanopore sequencing library building of the respiratory pathogens. According to the method, the library building purpose can be achieved only through the two steps of PCRamplification and joint connection, and during the PCR amplification in the second step, it is only required to supplement with primers without adding enzymes, so that the operation steps are simple;the characteristic of rapid library building is achieved so that real-time sequencing and analysis can be realized; and meanwhile, a bacterial metagenome and a fungal metagenome in a sample are detected.

The invention discloses a multi-fluorescent PCR rapid detection kit for novel coronavirus, influenza A virus and influenza B virus. The kit comprises freeze-dried solid RT-PCR Mix, liquid redissolution Buffer, freeze-dried solid positive control and freeze-dried solid negative control, wherein the freeze-dried solid RT-PCR Mix contains a primer group corresponding to primer sequences of a SARS-CoV-2 specific gene ORF1ab, an influenza A M gene, an influenza B M gene and a human reference gene RNAse P. The kit combines a multi-fluorescent quantitative PCR technology and a freeze-drying process, utilizes three pairs of special primers and human reference genes to amplify specific sequences of three pathogens in vitro, and performs real-time detection in combination with a fluorescent probe. The detection method is simple and convenient to operate, has low requirements for the operation level of detection personnel, and can detect three common respiratory pathogens at a time, the detection time and the detection cost are greatly saved, rapid screening of large-batch samples is realized, the whole detection process only takes 40 minutes to 1 hours, and results are accurate and reliable.

A self-sterilizing, face mask, respirator, open or enclosed face shield employs germicidal, far UV-C light safe for direct human exposure, mitigating risk of acquisition or transmission of respiratory pathogens, such as COVID 19. Device attachments or special lenses direct far UV-C light into the respiratory chamber and / or reservoir areas of infection including nasal, oropharyngeal or ocular areas to reduce or eliminate pathogens, serving also as a treatment modality. In a preferred embodiment the face shield has a removable front section, which can be exchanged or replaced with sections that include various functional attributes while maintaining germicidal protections and avoiding need to remove PPE during these activities including consumption of liquids, oral medications or food, as well as providing antimicrobial absorbent sneeze or cough guard or rhinorrhea secretion absorption, or a communication device to address communication deficiencies caused by respiratory protection barriers.

The invention belongs to the field of medical detection, and particularly relates to primers and probes for detecting SARS-CoV-2 and application of the primers and the probes. Four groups of primers and probes are designed, the SARS-CoV-2 is detected by adopting a common TaqMan-MGB probe method, and the primers and probes can be used for detecting a SARS-CoV-2 wild type, a SARS-CoV-2 variant, a SARS-CoV-2 B.1.617 subtype variant and a SARS-CoV-2 B.1.617.2 subtype variant. Pseudovirus test results show that the sensitivity of a reagent can reach 500 copies / mL. No non-specific amplification exists in detection of 10 healthy volunteers and 4 common other respiratory pathogens, and the primer and probe combination and the reagent have good specificity.