102 results about "Respiratory pathogen" patented technology

The invention relates to the technical field of biomedicine, in particular to a primer probe combination for broad-spectrum screening of common respiratory pathogens and application of the primer probe combination. Through screening and optimization, the respiratory pathogen nucleic acid detection primer probe combination for broad-spectrum screening of common acute respiratory infection and community-acquired pneumonia is obtained; the primer probe combination covers 18 respiratory tract pathogens, has the advantages of high accuracy, good specificity, strong anti-interference capability, good inclusiveness, high sensitivity, hyperjoint detection and the like, can realize parallel detection of 20 targets in cooperation with totally-closed single-tube PCR amplification, greatly improves early diagnosis of respiratory tract diseases with complex pathogen structures, and has wide application prospects. The method has very important significance on prevention, control and treatment of infectious diseases.

The invention discloses a respiratory infectious disease multi-pathogen nucleic acid detection kit based on a fluorescent PCR detection technology and application of the respiratory infectious disease multi-pathogen nucleic acid detection kit. The invention provides two detection kits aiming at 20 and 6 respiratory tract detection, the two kits can specifically detect respiratory tract pathogens, the detection sensitivity is high, the whole detection system is short in time consumption and simple and convenient in use process, and detection results of 20 pathogenic microorganisms can be reported within 40 min at the shortest; the whole set of detection kit and the detection method are high in sensitivity and good in specificity; the normal-temperature storage and transportation of the reagent can be realized.

The invention discloses a nucleic acid combination product, a kit and a method for detecting various respiratory tract pathogenic bacteria. The nucleic acid combination product comprises a primer pair and a probe, wherein the sequences of the primer pair and the probe are respectively shown as SEQ ID NO: 1-SEQ ID NO: 24. The nucleic acid combination product realizes synchronous detection of various pathogens through a one-tube eight-color fluorescent PCR, freeze-drying and ultrasonic direct diffusion method technology, significantly reduces experimental operation steps, shortens detection time, reduces the complexity of result interpretation, and has strong anti-interference capability and high sensitivity.

The invention discloses a method and system for detecting respiratory tract pathogens, pathogen drug-resistant genes and pathogen virulence genes, and relates to the technical field of gene detection.The method comprises the steps that a respiratory tract sample of a patient is collected, DNA and RNA are extracted, and total nucleic acid is obtained; carrying out first-round PCR amplification on the total nucleic acid, purifying a first-round PCR product, carrying out second-round PCR amplification, and sorting the product to obtain a library building product; performing high-throughput sequencing on the library building product to obtain sequencing data, and detecting pathogens, drug-resistant genes and virulence genes; according to the method and system for detecting the respiratory tract pathogens, the pathogen drug-resistant genes and the pathogen virulence genes, by optimizing library preparation and direct library building of nucleic acid, the process is simplified, and efficiency and accuracy are improved; the balance reagent design guarantees library quality; the kit adapts to pathogen detection requirements in Yunnan and the like, provides efficient diagnosis, shortens detection time, improves treatment effect, regularly updates pathogen information, and keeps technical frontier.

The invention designs a carbapenem drug-resistant gene nucleic acid fluorescent PCR method detection kit and application thereof, the kit uses Taqman fluorescent probe multiple amplification technology to detect carbapenem drug-resistant genes, the kit comprises a nucleic acid reaction liquid Mix1 and a nucleic acid reaction liquid Mix2, the nucleic acid reaction liquid Mix1 is used for detecting drug-resistant genes IMP, NDM and OXA48, the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48, and the nucleic acid reaction liquid Mix2 is used for detecting drug-resistant genes IMP, NDM and OXA48. The nucleic acid reaction liquid Mix2 is used for detecting drug resistance genes VIM, KPC and OXA23. The method is high in detection specificity, and the detection result is visual and easy to interpret. Clinical doctors are assisted to judge respiratory tract pathogen infection more comprehensively and more accurately. The kit covers six carbapenem drug resistance genes, is more comprehensive and accurate in detection, and is high in specificity and sensitivity. Meanwhile, multiple sample types can be detected, a sputum sample, an excrement sample and a rectum swab sample are detected through paramagnetic particle extraction, and pure bacterial colonies are directly detected without extraction.

The invention discloses a nucleic acid combination product for detecting various respiratory pathogens, a kit containing the nucleic acid combination product and a method. The nucleic acid combination product comprises a primer pair and a probe, wherein the nucleotide sequences of the primer pair and the probe are respectively shown as SEQ ID NO: 1-21. According to the nucleic acid combination product, on the technical basis of Taqman fluorescent quantification, seven targets can be detected by one tube, so that the time and energy of operators are greatly shortened, and the nucleic acid combination product is convenient and rapid, is not easy to make mistakes, and is simpler and easier to understand in result interpretation.

The invention discloses a liquid-phase hybridization probe pool for joint detection of multiple pathogens and drug-resistant genes. The liquid-phase hybridization probe pool is designed according to target areas of target pathogens and drug-resistant genes; the probe is a probe with unequal length of 30 to 100 nt; comprising a target specific sequence complementarily paired with a target region and a conjugate sequence complementarily paired between adjacent probes; the 5'terminal and the 3 'terminal of the probe are respectively modified with a conjugate sequence A and a conjugate sequence B, so that the adjacent probes can be specifically combined through terminal pairing; the conjugate sequence A is as shown in SEQ ID No. 601, and the conjugate sequence B is as shown in SEQ ID No. 602. According to the method, the constructed library and the specific probe pool are hybridized, so that the capture time is shortened to 30 minutes, and the method is obviously superior to a traditional method. Through optimization, the operation time and complexity are reduced, personal errors are reduced, meanwhile, high sensitivity and specificity are kept, target nucleic acid in a complex sample can be efficiently captured, a high-quality template is provided for subsequent sequencing, and combined detection of multiple respiratory pathogens and drug-resistant genes is supported.

The invention relates to the field of public health, in particular to an intelligent identification and real-time early warning system for unknown pathogens in respiratory tract samples of fever outpatient service, which comprises a data quality control processing module for performing quality control and standardized preprocessing on metagenome original sequencing data to obtain pure microorganism reading length; the known pathogen identification and annotation module is used for identifying known pathogens, classifying non-host reads, constructing a classification database according to a classification result, and annotating reads with low classification confidence or unclassified reads in the classification result to obtain an annotation table; the recognition model training module is used for constructing a positive and negative sample data set and training a recognition model; and the unknown pathogen identification module is used for identifying potential unknown pathogens in the metagenome of the pure microorganism read length by adopting the trained identification model according to the classification database and the annotation list. According to the invention, identification, classification and dynamic monitoring of known and unknown respiratory pathogens can be realized.

The invention discloses a primer composition for respiratory tract pathogen detection, a related product and application, and belongs to the field of pathogen detection. The primer composition comprises a primer pair aiming at influenza A virus, a primer pair aiming at influenza B virus, a primer pair aiming at bordetella pertussis, a primer pair aiming at adenovirus, a primer pair aiming at respiratory syncytial virus and a primer pair aiming at mycoplasma pneumoniae. The primer composition and related products can be used for simultaneously detecting influenza A viruses (seasonal H1N1, novel influenza A H1N1 viruses (2009), H3N2, H5N1 and H7N9), influenza B viruses (Victoria series and Yamagata series), adenoviruses (group B, group C and group E), respiratory syncytial viruses (subtype A and subtype B), mycoplasma pneumoniae and bordetella pertussis, so that pathogenic pathogens are quickly determined, and the primer composition and related products have important significance on clinical detection.

The invention relates to the technical field of biology, in particular to a primer probe combination product for respiratory tract infection detection. A plurality of specific primers and self-quenching probes are integrated in a single reaction system; simultaneous, rapid and rapid detection on 15 respiratory pathogens (including influenza A virus, influenza B virus, respiratory syncytial virus, severe acute respiratory syndrome coronavirus 2, mycoplasma pneumoniae, parainfluenza virus, rhinovirus, enterovirus, coronavirus, adenovirus, metapneumovirus, bordetella pertussis, bocavirus, chlamydia pneumoniae and chlamydia psittaci) is realized. The detection is accurate.

The invention discloses a primer probe combination and a kit for detecting nucleic acids of 14 respiratory pathogens and application, and belongs to the technical field of pathogen detection. The technical problem to be solved is that the detection of respiratory pathogens at the present stage is not only limited by a complex operation process, but also is difficult to meet the clinical rapid diagnosis requirement. Meanwhile, due to the inherent technical bottleneck, the problems of insufficient sensitivity, high false negative rate and the like exist. According to the technical scheme, the primer probe combination for simultaneously detecting 14 respiratory pathogens is provided, and based on the Real-time PCR technology, rapid, specific and accurate detection of the 14 respiratory pathogens is achieved.

The application discloses a set of molecular targets, primer compositions, methods and applications for identifying respiratory pathogens, the respiratory pathogens are novel coronavirus, influenza A virus and respiratory syncytial virus, and nucleotide sequences of the molecular targets are shown as SEQ ID NO:1-3. The application provides molecular targets, primers and probes for detecting three respiratory pathogens, and establishes a triple real-time RT-RAA detection technology, which can realize rapid identification and differentiation of three respiratory infectious disease pathogens with similar clinical symptoms within 30 minutes, reduces the operation threshold of the existing detection technology, realizes the target of rapidly, simply and accurately identifying and differentiating the three viruses, meets the needs of early clinical diagnosis of infection, provides technical support for precise medication, and has wide application value.

The present invention provides dual-target site reverse transcription fluorescent PCR primers, probes, and a kit for detecting the novel coronavirus SARS-CoV-2. By performing nucleic acid sequence alignment on the entire genome of the novel coronavirus SARS-CoV-2, three specific nucleic acid sequences W1, W2, and W3 in the viral N gene are found, and the sequences are shown as SEQ ID NOs. 1-3, respectively. Multiple pairs of primers and probes are designed for the three target sites, respectively. It is found that the dual-target site reverse transcription fluorescent PCR using a primer-probe combination designed for the W1 and W2 targets (sequences shown as SEQ ID NOs. 4-6 and SEQ ID NOs. 7-9, respectively) can specifically and sensitively detect the novel coronavirus SARS-CoV-2, with a detection sensitivity of less than 10 copies. There is no non-specific amplification of 22 common clinical respiratory pathogens, which can effectively avoid false negative results caused by single target site mutations and has good specimen detection capabilities.

The application discloses a nucleic acid combination product, a kit and a method for detecting multiple respiratory tract pathogenic bacteria. The nucleic acid combination product comprises a primer pair and a probe, wherein the sequences of the primer pair are respectively shown as SEQ ID NO:1-SEQ ID NO:24. The nucleic acid combination product realizes the synchronous detection of multiple pathogenic bacteria through one-tube eight-color fluorescence PCR, freeze-drying and ultrasonic direct extension technology, significantly reduces the experimental operation steps, shortens the detection time, reduces the complexity of result interpretation, and has strong anti-interference ability and high sensitivity.

The invention relates to the technical field of molecular biological detection, in particular to a multiplex digital PCR (polymerase chain reaction) kit for detecting various respiratory pathogens, which comprises a primer pair aiming at various targets and a unique fluorescence proportion coding probe group, each group of probes comprises probes marked with two fluorophores with different proportions, and high-throughput identification of multiple targets in a single reaction is realized through fluorescence proportion combination. The kit is designed by adopting a gradient reaction system, primer pairs are grouped according to a non-interference principle, and concentration is adjusted according to target expected abundance differentiation, so that amplification bias is effectively inhibited, and quantitative accuracy in a wide dynamic range is ensured. The invention also provides a complete detection scheme containing the internal standard system, and is suitable for rapid, accurate and multiple absolute quantitative detection of respiratory tract infection pathogens.

The application discloses a swab collecting and transporting device for respiratory pathogen detection and relates to the technical field of medical devices.The device comprises a tube body and a tube cover, and the tube body is composed of an outer tube body, an inner container and a vacuum heat preservation layer; the inner container is provided with a U-shaped breaking gap at the container mouth, the gap is provided with a wedge-shaped cutting edge, and a limiting block provided with anti-skid serrations is arranged below; the container mouth is provided with a sealing flow blocking block and a rubber plug body, the plug body is provided with a medical hydrophilic liquid absorbing composite buffer layer at the bottom; and the outer tube body is provided with a real-time temperature display unit, which can adopt reversible heat-sensitive color-changing strips or waterproof miniature digital display modules.The wedge-shaped cutting edge and the limiting structure are used to realize accurate and labor-saving breaking of the swab, so that sample spatter and pollution are avoided; multi-stage sealing and liquid absorbing buffer layer double anti-overflowing can quickly absorb and inactivate leaked samples; vacuum heat preservation is combined with real-time temperature monitoring to guarantee the cold chain storage environment of the samples.The whole operation is convenient and safe, the device is suitable for clinical sampling processes, and the reliability of respiratory pathogen sample collection, preservation and transportation can be effectively improved.

Provided are methods for inducing immune responses against pathogens in a mammal. The methods comprise administering a single cycle adenovirus (SC-Ad) comprising a nucleic acid sequence encoding an immunogen of said pathogen to said mammal. The SC-Ad is administered by inhalation and infects a cell of said mammal, thereby causing expression of said immunogen leading to the induction of said immune response. New infectious virions are not produced. The methods may be of particular utility in vaccination for respiratory pathogens. Corresponding medical uses of the SC-Ads are also provided.

The invention relates to the field of biological medicines, and discloses application of a GOSR1 gene in prevention and / or treatment of respiratory pathogens. The GOSR1 gene is specifically knocked down, so that the gene and protein expression level of the pathogen can be reduced, and the proliferation of the respiratory pathogen can be remarkably inhibited. In addition, the invention also discloses that overexpression of the GOSR1 gene can activate proliferation of pathogens in cells infected by respiratory pathogens. Based on the regulation effect of the GOSR1 gene on respiratory tract pathogen proliferation, a brand new strategy is provided for treatment of respiratory tract pathogen infection, and the application is particularly suitable for intervention of influenza A virus infection.

The application provides a quality control for detecting respiratory pathogen nucleic acid, which comprises any one or more of the following respiratory pathogens: coronavirus, influenza virus, adenovirus, Mycoplasma pneumoniae, Streptococcus pneumoniae, rhinovirus or / and Legionella pneumophila. The full-length genome sequence of the coronavirus is divided into 6 target fragments, each with a length of 4000-5500 bp. The quality control of the application covers all detection target sequences (or target points) of the coronavirus, has a wide coverage, comprehensively detects target points, and does not have the phenomenon of missed detection. The quality control of the application contains main respiratory pathogen infections, has a wide range, and uses real virus samples and lentivirus samples as raw materials, and has more accurate detection target points. The preparation of the coronavirus quality control of the application integrates the target gene sequence into the host genome through the lentivirus vector, knocks out the self-replication gene in the preparation process, and has a "self-inactivation" ability, so that the recombinant lentivirus cannot replicate in the target cells and infect other cells.

The invention belongs to the technical field of molecular detection, and particularly relates to a respiratory tract pathogen detection kit based on capillary electrophoresis and application of the respiratory tract pathogen detection kit, the kit can detect common pathogens which are possibly infected by respiratory tract patients and cannot be detected and determined through a conventional technology or a kit, and the kit at least has the technical advantages of being high in sensitivity and specificity.

The invention relates to the technical field of biomedical detection, in particular to a multiple TMA-CRISPR detection kit aiming at respiratory pathogen RNA and application of the multiple TMA-CRISPR detection kit, and solves the problem of weak multiple detection sensitivity and signal recognition during CRISPR multiple detection.The multiple TMA-CRISPR detection kit is obtained by firstly screening specific TMA primers and crRNA of IAV, IBV, RSVA and RSVB; a TMA-CRISPR system containing a specific enzyme proportion and a buffer solution is optimized, TMA amplification and Cas12a2 side cutting activity are combined, the constructed multiple TMA-CRISPR kit can achieve 10 copy / microliter RNA detection within 40 minutes, a clinical detection result is highly consistent with qRT-PCR and is more sensitive, and the kit is suitable for rapid multiple diagnosis of respiratory pathogens.

The invention discloses a reagent combination and a kit for detecting four respiratory tract pathogens based on qPCR, and belongs to the technical field of microbiological detection. Wherein the four respiratory tract pathogens are respectively human adenovirus, human bocavirus, bordetella pertussis and mycoplasma pneumoniae; the kit also comprises an optimized PCR (Polymerase Chain Reaction) buffer solution, DNA (Deoxyribose Nucleic Acid) polymerase and UDG enzyme. Through screening processes such as specific primer probe design, reagent formula and reaction system optimization, high sensitivity and high specificity are maintained while rapid detection is performed, and missing detection and misjudgment are effectively avoided.

The invention belongs to the technical field of pathogen molecular diagnosis, and particularly relates to a mycobacterium tuberculosis detection primer group, method and kit based on multiple PCR (Polymerase Chain Reaction) and high-throughput sequencing. The primer combination aiming at a plurality of high-copy and single-copy gene areas of the mycobacterium tuberculosis is designed through a unique bioinformatics process, and the capturing capacity and amplification efficiency of a low-bacterium-amount sample are fundamentally improved. A pollution discrimination rule based on multi-target consistency verification is established by using NGS multi-target data features, so that pollution identification is internalized into a part of a data analysis process. According to the invention, the optimized mycobacterium tuberculosis specific primer group and the primer pool covering other common respiratory pathogens are integrated in the same multiplex PCR reaction system, so that synchronous and efficient detection of mycobacterium tuberculosis and other pathogens is realized, and the diagnosis efficiency and range are greatly improved.

The application discloses a kind of absolute quantitative detection method of child respiratory tract multi-pathogen based on multi-target ladder microarray and exogenous internal reference correction.The method sets up independent micro-reaction unit to different pathogen, adds fixed amount of exogenous internal reference nucleic acid, and calculates template number by positive unit proportion combined with Poisson distribution model, then corrects the result by using the theoretical addition amount of exogenous internal reference, so as to realize parallel detection and absolute quantification of pathogen such as bocavirus, adenovirus, mycobacterium tuberculosis and mycoplasma pneumoniae without standard curve and special digital PCR equipment.The application has the advantages of high sensitivity, strong anti-interference ability, suitable for mixed infection sample analysis and clinical application, etc.

The invention relates to a medical xenon recovery system based on multistage filtration and dynamic separation. The medical xenon recovery system comprises a pretreatment and microorganism inactivation module, a gas purification and separation module, a xenon circulation distribution module, an intelligent monitoring system and a dynamic pressure compensation mechanism. The invention further relates to a medical xenon recovery method based on multi-stage filtration and dynamic separation, bacteria are intercepted through the filter screen, viruses are intercepted through large particles, short-wave sterilization ultraviolet LED array irradiation is combined, double barriers of physical interception and ultraviolet inactivation are formed, the method is far superior to a traditional single filtration technology, the grade cleaning standard is met, and the medical xenon recovery method has the advantages of being simple in operation, low in cost and high in efficiency. The system thoroughly solves the biological potential safety hazard of lack of ultra-small virus interception and inactivation in the prior art, can efficiently inactivate respiratory pathogens, can effectively prevent microorganisms in exhaled air from circulating to patients or medical staff through a recovery system, is especially suitable for infectious surgery scenes, and remarkably reduces the risk of cross infection in hospitals.

The invention relates to a household self-test respiratory pathogen nucleic acid detection method and device. The method comprises four steps of sample collection, sample treatment, amplification detection and result interpretation. The device comprises a sample collection device, a sample treatment module, a temperature control module and an amplification detection module. The detection method and device are simple to operate, high in sensitivity, good in specificity, low in cost, free of other electronic components and suitable for rapid detection of household respiratory pathogens, and a result can be rapidly obtained without complex equipment.

The invention belongs to the technical field of pathogen detection, and particularly relates to a freeze-drying PCR reagent, a kit and a method for detecting nucleic acids of six respiratory pathogens. Through optimized and screened freeze-drying stabilizer combination, key components such as primers and probes corresponding to each target and an internal standard, a buffer system and the like are successfully prepared into freeze-dried powder with stable performance under the condition of 2-8 DEG C through a freeze-drying process, and meanwhile, the freeze-dried powder has high sensitivity and high specificity in detection of pathogens, and has a good application prospect. The condition requirements and the cost of the fluorescent PCR reagent in the logistics transportation and storage process are obviously reduced.

This utility model discloses a respiratory suction device, relating to the field of respiratory medicine technology. It includes a device base plate, with an annular fixing assembly fixedly connected to the top of the base plate. A suction container is inserted inside the annular fixing assembly, and a protective cover is inserted into the top of the suction container. A sealing ring is inserted inside the protective cover. Through the sealing ring and the filtration and purification assembly, this utility model achieves safety and filtration effects during operation. The filtration and purification assembly uses multi-layer filtration. The first layer, a polypropylene membrane, with its large pore size, quickly captures larger particulate impurities in the sputum. Two subsequent layers of cellulose filter cotton provide deep purification, intercepting tiny bacteria, viruses, and harmful aerosols. It can filter a large number of respiratory pathogens, greatly reducing the risk of cross-infection in wards. The sealing ring at the bottle opening is made of highly elastic, aging-resistant medical silicone, effectively preventing leakage and improving safety.

The application relates to the technical field of molecular biology detection, in particular to a nucleic acid combination product for identifying respiratory tract pathogens, a PCR premix, a kit and a method. The nucleic acid combination product comprises primer pairs and probes shown in SEQ ID NO: 1 to SEQ ID NO: 21. The primer pairs and the probes can be mixed with other reagents required by PCR, and then freeze-dried to prepare a freeze-dried ball. The freeze-dried ball is smooth and round in surface, and after a nucleic acid sample liquid to be detected is directly dropped into a PCR reaction tube containing the freeze-dried ball, machine detection can be carried out. The freeze-dried ball is not only beneficial to storage and transportation, but also convenient to operate. In particular, the effect of joint detection of target pathogens is good, and the detection sensitivity is also improved.

The present application relates to the field of molecular biology detection, in particular to a fluorescent PCR kit and a detection method for respiratory virus detection. The present application constructs specific primer groups and probes for detecting respiratory viruses, including influenza A virus, influenza B virus, human parainfluenza virus type I, human parainfluenza virus type II, human parainfluenza virus type III, respiratory syncytial virus and metapneumovirus. Through a matching PCR system, the simultaneous detection of multiple respiratory pathogens is realized in a single-color detection channel by using the melting curve and the characteristic peak of the melting temperature as indicators. The present application at least doubles the existing fluorescent PCR detection throughput, has low cost, short time consumption, greatly improves the detection efficiency, and can be widely used for mixed detection and analysis of multiple pathogens. The detection results can be further used for assisting clinical diagnosis and treatment.