91results about How to "Solve easy pollution" patented technology

The invention discloses a multi-gene detection kit for guiding administration of 5-fluorouracil and a detection method of the multi-gene detection kit. The multi-gene detection kit comprises ultrapure water, an X solution, a 10*PCR (Polymerase Chain Reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (Deoxyribonucleic Acid) polymerase and a positive control, and is characterized in that the PCR primer comprises six forward and reverse amplification primers with different genotypes on seven SNP (Single Nucleotide Polymorphism) sites of genes related to the administration of 5-fluorouracil, and forward and reverse amplification primers of a DNA internal reference and a reaction internal reference, and gene sequences of the forward and reverse amplification primers are shown as SEQ ID NO. 1-32 (Sequence Identifier Numbers 1-32). The detection method of the multi-gene detection kit comprises the steps of collecting a sample, extracting nucleic acid, conducting a PCR by taking the extracted nucleic acid as a template, and conducting capillary electrophoretic separation on the sample with a GeXP genetic analyser. The multi-gene detection kit and the detection method have the advantages of strong specificity, high accuracy, flux and reliability, low cost and no false negative result.

The invention discloses a kit for synchronously detecting twenty-two respiratory tract pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the nine respiratory tract pathogens, and a reverse primer of a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-6 (sequence identifier number 1-6), and the PCR primer comprises forward and reverse PCR amplification primers of the rest thirteen respiratory tract pathogens, a forward and reverse amplification primer of a human DNA internal reference, a forward and reverse PCR amplification primer of a reaction internal reference, PCR amplification primers of the nine respiratory tract pathogens, and a PCR amplification primer of the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 7-44. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.

The invention provides automatic transfusion medicine dispensing equipment, which belongs to the technical field of transfusion medicine dispensation. The automatic transfusion medicine dispensing equipment can automatically extract liquid in an ampoule, medicine powder or water aqua in a powder bottle, and mother liquid in a mother liquid container to be mixed. The invention also provides an automatic transfusion medicine dispensing method. The equipment and the method in the invention can be used for realizing the effect of operating the liquid in the ampoule and the medicine powder or the water aqua in the powder bottle by a machine and mixing the liquid in the ampoule and the medicine powder or the water aqua in the powder bottle with the mother liquid, the problem of easy pollution of the medicine liquid is solved, the medicine dispensing risk is lowered, the disputes between doctors and patients can be reduced, and in addition, the invention saves labor and improves the nursing efficiency and quality.