Nucleic acid, kit and method for detecting polymorphism of human CYP2D6 gene

A gene polymorphism and kit technology, which is applied in the fields of biochemical equipment and methods, recombinant DNA technology, and microbial determination/inspection. The complex operation process of the phase chip method can avoid the problems of non-specific amplification, high accuracy and easy interpretation.
CN106755360AActive Publication Date: 2017-05-31武汉海吉力生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
武汉海吉力生物科技有限公司
Publication Date
2017-05-31

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Abstract

The invention discloses nucleic acid, a kit and a method for quickly detecting polymorphism of a human CYP2D6 gene, which are particularly used for detecting site polymorphism of CYP2D6*10 genes C100T and G4268C as well as a CYP2D6*14 gene G1758A. The detection kit for quickly detecting the polymorphism of the CYP2D6 gene by using real-time fluorescent quantitative PCR, has remarkable advantages of high specificity, high sensitivity, short experiment cycle, simplicity in operation, safety, no toxicity, low cost and the like; the detection method is simple and convenient to operate and high in automation degree; the operation process is greatly simplified, pollution in the operation process is reduced, and the detection result is accurate and reliable.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, and in particular relates to a nucleic acid, a kit and a method for detecting human CYP2D6 gene polymorphism. Background technique

[0002] CYP2D6 is one of the important members of the CYP enzyme family. The gene is located at 22q13.1 and contains 9 exons and 8 introns, with a total length of about 7kb. It is a complete functional gene. Studies have shown that CYP2D6 in the liver only accounts for 2-9%, but it is involved in the metabolism of 20% to 30% of drugs, including antidepressants, antiarrhythmic drugs, antipsychotics, and analgesics. CYP2D6 has a wide range of gene polymorphisms, and more than 100 gene variants have been reported, such as single base mutation, deletion or insertion and deletion of large fragments. These genetic variations can cause differences in enzyme activity and quantity, leading to individual differences in drug metabolism. The metabolic phenotypes of CYP2D6 can be d...

Claims

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