Kit for synchronously detecting twenty-three meningitis pathogens and detection method of kit

Abstract

The invention discloses a kit for synchronously detecting twenty-three meningitis pathogens and a detection method of the kit. The kit comprises DEPC (diethylpyrocarbonate) water, a 5*RT (reverse transcription) buffer, a reverse transcription primer, a reverse transcriptase, an X solution, a 10*PCR (polymerase chain reaction) buffer, a PCR primer, a 25mM magnesium chloride solution, a DNA (deoxyribonucleic acid) polymerase and a positive control, and is characterized in that the reverse transcription primer comprises RT amplification primers of the twelve meningitis pathogens and a human RNA (ribonucleic acid) internal reference, and has a gene sequence shown as SEQ ID NO. 1-13 (sequence identifier number 1-13), and the PCR primer comprises forward and reverse PCR amplification primers of the rest eleven meningitis pathogens, a human DNA internal reference and a reaction internal reference, and PCR amplification primers of the twelve meningitis pathogens and the human RNA internal reference, and has a gene sequence shown as SEQ ID NO. 14-52. The kit and the detection method have the advantages of high specificity, sensitivity, flux and reliability, low cost, and no false negative results.

Description

technical field [0001] The present invention relates to a detection kit for pathogens of encephalitis and meningitis and a detection method thereof, in particular to a kit for synchronously detecting twenty-three pathogens of encephalitis and meningitis based on a GeXP multiple gene expression genetic analysis system and detection thereof method. Background technique [0002] Brain fever (meningitis) is an infection of the delicate meninges, or meninges (the membrane between the skull and the brain). The disease is often accompanied by complications from bacterial or viral infection of any part of the body, such as ear, sinus, or upper respiratory infections. The pathogens causing encephalitis and meningitis are diverse, and the scope and degree of lesions vary greatly, and the clinical manifestations are very complicated. The current clinical diagnostic methods such as EEG, imaging, pathogen culture, serological examination and other methods cannot quickly and accurately ...

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