66results about How to "Rapid Qualitative Detection" patented technology

The invention relates to a typing detection kit of a multi-PCR fluorescence labelled probe for FK506 personalized medication related genes. According to three specific SNP sites, with high pertinence to metabolism of FK506, on CYP3A4 and CYP3A5 encoding genes, three pairs of amplification primers and three fluorescent probes are designed, a PCR instrument is adopted to amplify to-be-detected three segments of DNA sequences, hybridization of the fluorescent probes and amplified products is utilized to detect hybridization peaks in a reaction system, and Tm values displayed on the standard hybridization peaks for gene typing are determined as the result judgment standards by construction and detection based on wild-type and mutant standard plasmids. The typing detection kit is high in method flexibility, strong in specificity, simple and convenient to operate, and straightforward and clear in result observation, and two genetypes have significant differences and are easy to type. The typing detection kit is suitable for one-tube triple quick detection of three SNP sites of FK506 metabolism related genes.

The invention discloses an animal epidemic disease three-color fluorescence RT-PCR detection kit and a detection method thereof, and is characterized in that the kit comprises multiple fluorescence RT-PCR reaction mother liquor, a positive control, a negative control, an AMV reverse transcriptase, and a Taq polymerase, wherein the multiple fluorescence RT-PCR reaction mother liquor contains a multiple 10*fluorescence RT-PCR reaction buffer, an avian influenza primer and a probe, a newcastle disease virus primer and a probe, an avian infectious bronchitis primer and a probe, and bovine serum albumin, wherein sequences of the forward primers, reverse primers, and specific probes are as shown in SEQIDNO. 1, NO. 2, NO.3, NO.4, NO.5, NO.6, NO.7. NO.8, NO.9, and NO.10. The advantages of the invention are that avian influenza, newcastle disease, and avian infectious bronchitis viruses can be detected simultaneously in a same reaction tube; the specificity is strong; the sensitivity is high, is rapid and accurate.

The invention discloses a detection method for heme based on the fluorescence quenching effect of boron-doped grapheme quantum dots (B-GQDs). The detection method comprises the following steps: 1) preparing a B-GQDs solution; 2) preparing a heme solution; 3) preparing a standard solution; 4) creating a working curve for detecting the concentration of the heme with the B-GQDs; and 5) detecting the concentration of the heme in a sample to be detected. According to the detection method, based on the fluorescence quenching effect of the B-GQDs, the B-GQDs are firstly applied to detection for the heme, and the heme can be quickly and efficiently detected by using a fluorescence analyzing method. The detection method has the advantages of high response speed, high sensitivity, simplicity in operation, low cost, strong optics antijamming capability and the like; the content of the heme can be detected through the change of a fluorescence signal, so as to realize quick and sensitive qualitative detection and quantitative determination for the biochemical index of the heme.

The invention discloses an exosome surface protein detection method, which comprises the steps of first, extracting exosomes from a tumor cell culture medium by centrifugation; second, using a reportprobe to react with an exosome to be detected, wherein the report probe is a spherical gold nanoparticle, and the surface is connected with a protein adapter and a Raman molecular; and third, observing the color of the report probe or detecting an SERS signal of the report probe. According to the invention, a colorimetric method and an SERS detection technology are simultaneously applied to the exosome surface protein detection system, firstly the sample is quickly analyzed by adopting the colorimetric method, and then the SERS technology is utilized to achieve accurate quantitative analysis for the exosome surface protein, so that fast and high-sensitivity detection for the exosome surface protein is realized by combining the two methods.

The invention discloses an immune colloidal gold test stripe for detecting leucomalachite green in aquatic products and a preparation method; the colloidal gold test stripe provided by the invention detects leucomalachite green with a direct competition method, and comprises a sample pad, a colloidal gold combined pad, a nitrocellulose membrane (NC), a water adsorption pad and a PVC back lining, one end of the PVC back lining is sequentially adhered with the sample pad and the colloidal gold combined pad, and the middle thereof is adhered with the NC layer, and the other end thereof is sequentially adhered with water adsorption pad; the immune colloidal gold test stripe has the characteristics that colloidal gold combined pad is enveloped with colloidal gold marker which is monoclonal antibody of the specificity of the leucomalachite green, and the NC is adsorbed with leucomalachite green-OVA and sheep anti-mouse IgG. The invention has high detection sensitivity and low price and is simple and rapid, the whole reaction only needs 30 minutes; the immune colloidal gold test stripe can be used for large-batch screening of samples and can rapidly detect the residual leucomalachite green in the aquatic products at site.

The invention provides a nucleic acid qualitative detection method based on nano-particles and rolling circle amplification. The detection method mainly comprises the steps of: allowing silica nanoparticles in a disperse state in a solution before the rolling circle amplification is used; performing the rolling circle amplification when a target nucleic acid molecule is present; and gathering the silica nanoparticles to form a macroscopic white blocky substance in the solution so as to achieve the purpose of qualitatively detecting the target nucleic acid molecule. As the presence of the target nucleic acid molecule can be judged by gathering states of the silica nanoparticles before and after rolling circle amplification, a large number of trace nucleic acid molecules are amplified, results are rapidly and intuitively detected without a special detection instrument, the detection cost is reduced, and the nucleic acid qualitative detection method is suitable for popularization of clinical detection.

A colorimetric identifying and detecting DNA method is disclosed. It has double functions of allochroic indicant group and reinforced allochroism by functional polymeric butadiyne vesiculation. It achieves simple and rapid process.

The invention relates to the technical field of bioengineering and discloses a VEGF (vascular endothelial growth factor) monoclonal antibody and a fracture healing evaluation antibody chip with the same. The amino acid sequence of a heavy chain variable region of the monoclonal antibody is shown as SEQ ID NO.1, and the amino acid sequence of a light chain variable region of the monoclonal antibody is shown as SEQ ID NO.2. Homogeneity and biological activity unicity of the monoclonal antibody enables antigen antibody reaction results to be convenient for quality control and beneficial to standardization and normalization. The chip with the monoclonal antibody can be directly applied to clinical therapeutic evaluation on fracture healing, solves the technical problems of time and labor consumption, poor result repeatability and the like of the prior art, has extremely high application value in the field of clinical treatment and has wide application prospect.

The invention provides a method for detecting lime substances in flour by near infrared spectroscopy, which comprises the following steps of: acquiring a near infrared spectrum of a flour sample to be detected; determining whether the flour sample to be detected contains the lime substances or not according to the near infrared spectrum characteristics of the lime substances and pure flour; if a determined result is that the flour sample to be detected contains lime substances, acquiring a near infrared spectrum of a standard series flour sample; establishing a quantitative correction model according to the near infrared spectrum of the standard series flour sample; and detecting the content of the lime substances in the flour sample to be detected according to the quantitative correction model. The method for detecting the lime substances in the flour by the near infrared spectroscopy is simple in steps and pollution-free, and can be used for detecting whether the flour contains the lime substances or not quickly and qualitatively; and multiple lime substances in the flour can be measured quickly and quantitatively by combining the near infrared spectrum of the standard series flour sample and the quantitative correction model, and the obtained results are accurate and reliable.

The invention provides an integrated totally-closed detection reaction tube. Specifically, the detection reaction tube comprises: an upper body, wherein the upper body is provided with a cover, a first cavity and a second cavity, the second cavity is located below the first cavity, the first cavity and the second cavity are provided with a fluid channel, and the fluid channel allows fluid to flow into the second cavity from the first cavity; and a lower body, and the lower body is provided with a detection cavity and a puncture component; The upper body and the lower body are hermetically connected together through a coupling structure. In addition, the invention further provides a corresponding detection method, a detection device and application. By means of the integrated totally-closed detection reaction tube, qualitative detection on bacteria and viruses containing target sequences and other nucleic acid-containing samples can be carried out quickly, conveniently and efficiently.

The invention discloses a detection method for rapidly detecting beta-stimulant and a paper-based immunosensor. The manufacturing method of the paper-based immunosensor comprises the steps of: S1, designing a pore plate model through drawing software, then, printing the pore plate model designed previously on filter paper rich in cellulose through hydrophobic wax by means of a color printer, placing the filter paper printed with the pore plate model on a magnetic heater, heating for 2 minutes, and cooling to room temperature to obtain a paper-based micro-pore plate; and S2, adding beta-stimulant coating antigen into each circular reaction area of the paper-based micro-pore plate, drying the beta-stimulant coating antigen, then adding BSA confining liquid into each circular reaction area of the paper-based micro-pore plate, and drying the BSA confining liquid to obtain the paper-based sensor. According to the invention, the convenient paper-based immunosensor is utilized, so that the beta-stimulant can be quickly and sensitively detected in a colorimetric manner, and powerful technical support is provided for field detection, supervision and the like of the beta-stimulant.

The invention provides a method for detecting permanganate radicals by a coumarin-based probe. The coumarin-based probe is 2-oxo-2hydrogen-benzopyran-7-R acid ester, the coumarin-based probe is obtained by condensation of 7-hydroxybenzopyran-2-ketone and acyl chloride, and the response group of the probe to the permanganate radicals is double bonds. The single probe has no fluorescence in a mixed solution of an organic solvent and water, and shows obvious fluorescence lightening response when being used for detecting the permanganate radicals, the response time is less than 3 seconds, and real-time detection of the permanganate radicals can be realized; and the detection sensitivity is high, and the detection limit is as low as 0.95 nmol/L. The method has high selectivity on the permanganate radicals, and the content of the permanganate radicals in a water sample and a non-standard explosive raw material in the environment can be accurately measured by utilizing a standard curve. The method has excellent selectivity and sensitivity, can be used for rapidly detecting the permanganate radicals on site, and has a wide application prospect.

The invention provides a method applying AlphaLISA to detect a dengue virus and corresponding combination and kit. An AlphaLISA detection composition comprises receptor bead coated with a capture antibody, a detection antibody marked with biotin and donor bead coated with streptavidin, and the capture antibody and the detection antibody are a pair of matched antibodies resistant to dengue virus NS1 protein. The composition serves as antigen for the NS1 protein, thereby having high specificity and sensitivity. The invention further discloses an AlphaLISA kit for detecting the dengue virus. Thekit comprises the AlphaLISA detection composition, a 384-hole microporous plate, a positive quality control product and a negative quality control product, the positive quality control product is matrix serum of the dengue virus NS1 protein, and the negative quality control product is a solution containing basic buffer. The microporous plate with many holes can be used for detection, and quick andqualitative detection of the dengue virus in high-throughput large samples can be realized.

The invention discloses a terahertz spectrum qualitative detection method for a student school uniform textile material, which comprises the following steps: preparing different textile material experiment samples of a student school uniform, obtaining terahertz absorbance spectrums of different textile materials by using a terahertz time-domain spectroscopy system, and extracting feature information of a grey-scale map of the terahertz absorbance spectrums by using a Krawtchouk moment; combining an improved particle swarm algorithm with a support vector machine to establish a qualitative model, obtaining a classification identification model of different textile materials; collecting the textile materials on the school uniform, obtaining the feature information of the textile materials on the school uniform, and sending the feature information of the textile materials on the school uniform into the classification identification model of the corresponding textile materials for qualitative detection. The terahertz spectrum can be used for carrying out sexual detection on the textile material of the school uniform, so that the school uniform quality of primary and secondary school students is ensured, and primary and secondary school student safety incidents caused by the school uniform quality are avoided.

The invention discloses a PVY single virus colloidal gold rapid test strip which is composed of a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a backing; the colloidal gold pad is coated with a colloidal gold-labeled specific PVY antibody, and the antibody is secreted from a hybridoma cell strain BALBc-15-8; the NC membrane is provided with a test line and a control line, the test line is coated with a specific PVY antigen, and the control line is coated with a colloidal gold-labeled secondary antibody. The test strip is rapid to operate, sensitive, accurate, low in cost and easy and convenient to operate for testing PVY in Guangdong tobacco-growing areas, achieves simultaneous multi-sample diagnosis in one-time sampling, is very suitable for conducting site primary screening on a large scale of samples and has an application value in practical production and a good application and popularization prospect.