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66results about How to "Rapid Qualitative Detection" patented technology
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Primer, probe and kit for detecting type-16 HPV (human papillomavirus)
ActiveCN105087827AComprehensive detectionQuick screeningMicrobiological testing/measurementMicroorganism based processesDiseaseHuman papillomavirus
The invention discloses a primer, probe and kit for detecting type-16 HPV (human papillomavirus). The primer and the probe are suitable for screening the HPV related diseases quickly, accurately and simply, do not cross-react with other viruses, are capable of detecting common high and low risk types of HPVs, two types of HPVs and 14 types of HPVs in total, cover a wide range of high risk types of HPVs and are more accurate and complete in detection than kits in the present market. The kit further comprises negative, positive and weakly positive quality controls and helps further increase detection accurateness.
Owner:北京鑫诺美迪基因检测技术有限公司
Typing detection kit for FK506 personalized medication related genes
InactiveCN106086192ALow specificitySensitive qualitative detectionMicrobiological testing/measurementDNA/RNA fragmentationPersonalizationFluorescence
The invention relates to a typing detection kit of a multi-PCR fluorescence labelled probe for FK506 personalized medication related genes. According to three specific SNP sites, with high pertinence to metabolism of FK506, on CYP3A4 and CYP3A5 encoding genes, three pairs of amplification primers and three fluorescent probes are designed, a PCR instrument is adopted to amplify to-be-detected three segments of DNA sequences, hybridization of the fluorescent probes and amplified products is utilized to detect hybridization peaks in a reaction system, and Tm values displayed on the standard hybridization peaks for gene typing are determined as the result judgment standards by construction and detection based on wild-type and mutant standard plasmids. The typing detection kit is high in method flexibility, strong in specificity, simple and convenient to operate, and straightforward and clear in result observation, and two genetypes have significant differences and are easy to type. The typing detection kit is suitable for one-tube triple quick detection of three SNP sites of FK506 metabolism related genes.
Owner:上海泽因生物科技有限公司
Animal epidemic disease three-color fluorescence RT-PCR detection kit and detection method thereof
InactiveCN102851392ARapid Qualitative DetectionAccurate and specific qualitative detectionMicrobiological testing/measurementMicroorganism based processesForward primerPositive control
The invention discloses an animal epidemic disease three-color fluorescence RT-PCR detection kit and a detection method thereof, and is characterized in that the kit comprises multiple fluorescence RT-PCR reaction mother liquor, a positive control, a negative control, an AMV reverse transcriptase, and a Taq polymerase, wherein the multiple fluorescence RT-PCR reaction mother liquor contains a multiple 10*fluorescence RT-PCR reaction buffer, an avian influenza primer and a probe, a newcastle disease virus primer and a probe, an avian infectious bronchitis primer and a probe, and bovine serum albumin, wherein sequences of the forward primers, reverse primers, and specific probes are as shown in SEQIDNO. 1, NO. 2, NO.3, NO.4, NO.5, NO.6, NO.7. NO.8, NO.9, and NO.10. The advantages of the invention are that avian influenza, newcastle disease, and avian infectious bronchitis viruses can be detected simultaneously in a same reaction tube; the specificity is strong; the sensitivity is high, is rapid and accurate.
Owner:NINGBO ACAD OF SCI & TECH FOR INSPECTION & QUARANTINE
Detection method for heme based on boron-doped grapheme quantum dots fluorescence quenching effect
InactiveCN105424664AGood biocompatibility and affinityEasy to operateFluorescence/phosphorescenceFluorescenceQuenching
The invention discloses a detection method for heme based on the fluorescence quenching effect of boron-doped grapheme quantum dots (B-GQDs). The detection method comprises the following steps: 1) preparing a B-GQDs solution; 2) preparing a heme solution; 3) preparing a standard solution; 4) creating a working curve for detecting the concentration of the heme with the B-GQDs; and 5) detecting the concentration of the heme in a sample to be detected. According to the detection method, based on the fluorescence quenching effect of the B-GQDs, the B-GQDs are firstly applied to detection for the heme, and the heme can be quickly and efficiently detected by using a fluorescence analyzing method. The detection method has the advantages of high response speed, high sensitivity, simplicity in operation, low cost, strong optics antijamming capability and the like; the content of the heme can be detected through the change of a fluorescence signal, so as to realize quick and sensitive qualitative detection and quantitative determination for the biochemical index of the heme.
Owner:NANJING NORMAL UNIVERSITY
Method of detecting ochratoxin A in cereals by means of nano antibody and immunomagnetic separation
The invention discloses a method of detecting ochratoxin A in cereals by means of a nano antibody and immunomagnetic separation. The method includes the steps of: 1) combining anti-OTA nano antibody with nano immunomagnetic beads to prepare IMB, and enriching the OTA in the cereals by means of the IMB to prepare a pretreated sample; 2) with dot immunization method, immobilizing an OTA-OVA detection antigen on a PVDF film, wherein the immunomagnetic beads are subjected to an immune-reaction in the form of antibody marker, the OTA-OVA and the to-be-tested substance, OTA, are combined with the antibody competitively so as to determine whether the immunomagnetic beads are aggregated on the PVDF film or not, thereby obtaining a detection result. The method achieves simple and quick qualitative detection. An operator can read the result just by naked eyes without any analysis instrument. Furthermore, the method can be used for developing a novel quick and visible detection card for specially detecting the OTA in the cereals.
Owner:HAINAN UNIVERSITY
Mass-spectrum rapid analysis method for pesticide residue in fruits and vegetables
ActiveCN104062306ARapid Qualitative DetectionImprove mobilityMaterial analysis using wave/particle radiationBiotechnologyPesticide residue
The invention discloses a mass-spectrum rapid analysis method for the pesticide residue in fruits and vegetables. A triple quadrupole mass spectrum is adopted as an analyzer, a discharging low-temperature plasma is used as a mass spectrum ionization source, and a sample is placed on a continuous sample introduction belt in front of the low-temperature plasma ionization source; a heating lamp is used for performing rapid non-contact thermal analysis of the sample; the sample is detected in an anion detection mode and compared with the early established spectrogram in a pesticide standard quality mass spectrum library to rapidly judge whether the residue of corresponding pesticide exists in the sample; the method disclosed by the invention improves the qualitative analysis ability and signal strength of the device; and compared with the traditional pesticide residue detection method, the method has the advantages of simple pretreatment, small dosage of organic solvent, short detection period of single sample and the like.
Owner:JING BRAND
Cell tissue resonance Raman spectroscopy scanning imaging method
InactiveCN104111247ARapid Qualitative DetectionEasy to operatePreparing sample for investigationRaman scatteringResonance Raman spectroscopyFluorescence
The invention discloses a cell tissue resonance Raman spectroscopy scanning imaging method which comprises the following step of: carrying out (1) freezing treatment and (2) sample preparation and scanning imaging on a cell tissue. The cell tissue resonance Raman spectroscopy scanning imaging method is easy and fast to operate, can fast carry out qualitative detection on a tissue sample, effectively enhances the Raman detection sensitivity, inhibits the fluorescence disturbance, enhances the Raman signal intensity, ensures the spectroscopy imaging quality, shortens the detection time and visually shows the distribution of characteristic components of the tissue sample in a clear image mode.
Owner:TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
Exosome surface protein detection method
InactiveCN109632765ARapid Qualitative DetectionHighly Sensitive Quantitative DetectionRaman scatteringColor/spectral properties measurementsNanoparticleCentrifugation
The invention discloses an exosome surface protein detection method, which comprises the steps of first, extracting exosomes from a tumor cell culture medium by centrifugation; second, using a reportprobe to react with an exosome to be detected, wherein the report probe is a spherical gold nanoparticle, and the surface is connected with a protein adapter and a Raman molecular; and third, observing the color of the report probe or detecting an SERS signal of the report probe. According to the invention, a colorimetric method and an SERS detection technology are simultaneously applied to the exosome surface protein detection system, firstly the sample is quickly analyzed by adopting the colorimetric method, and then the SERS technology is utilized to achieve accurate quantitative analysis for the exosome surface protein, so that fast and high-sensitivity detection for the exosome surface protein is realized by combining the two methods.
Owner:UNIV OF SHANGHAI FOR SCI & TECH
Immune colloidal gold test stripe for detecting leucomalachite green in aquatic product and preparation method
ActiveCN101655498ARapid Qualitative DetectionThe result is accurateOvalbuminSerum albuminNitrocelluloseAquatic product
The invention discloses an immune colloidal gold test stripe for detecting leucomalachite green in aquatic products and a preparation method; the colloidal gold test stripe provided by the invention detects leucomalachite green with a direct competition method, and comprises a sample pad, a colloidal gold combined pad, a nitrocellulose membrane (NC), a water adsorption pad and a PVC back lining, one end of the PVC back lining is sequentially adhered with the sample pad and the colloidal gold combined pad, and the middle thereof is adhered with the NC layer, and the other end thereof is sequentially adhered with water adsorption pad; the immune colloidal gold test stripe has the characteristics that colloidal gold combined pad is enveloped with colloidal gold marker which is monoclonal antibody of the specificity of the leucomalachite green, and the NC is adsorbed with leucomalachite green-OVA and sheep anti-mouse IgG. The invention has high detection sensitivity and low price and is simple and rapid, the whole reaction only needs 30 minutes; the immune colloidal gold test stripe can be used for large-batch screening of samples and can rapidly detect the residual leucomalachite green in the aquatic products at site.
Owner:江西中德生物工程股份有限公司
Gold nanopore array based SERS (surface-enhanced Raman spectroscopy) detection method for DNA methylation and application of method
ActiveCN108827935AWith Raman enhancement effectGood Raman enhancement effectRaman scatteringDNA methylationSurface-enhanced Raman spectroscopy
The invention discloses a DNA methylation detection method based on SERS (surface-enhanced Raman spectroscopy) of a gold nanopore array and an application of the method. The method comprises the following steps: (1) the periodic gold nanopore array is prepared by an electron beam lithography system through electron beam evaporation coating to serve as an SERS enhancing substrate; (2) surface Ramanenhancing performance of the SERS enhancing substrate is inspected by a Raman beacon molecule Rhodamine 6G, and the enhancing substrate is applied to DNA methylation detection; (3) DNA methylation detection based on a surface Raman enhancing effect is established. According to the detection method, the surface Raman enhancing performance of the prepared gold nanopore array is evaluated by the Raman beacon molecule Rhodamine 6G, and it is discovered that the gold nanopore array has good Raman enhancing effect on Rhodamine 6G and has efficient repeatability; the gold nanopore array is applied to DNA methylation detection. The method has the advantages of being high in sensitivity, simple to operate, high in repeatability and capable of being used for detecting DNA methylation.
Owner:NANJING NORMAL UNIVERSITY
Nucleic acid qualitative detection method based on nano-particles and rolling circle amplification
InactiveCN103014169AReduce testing costsHigh sensitivityMicrobiological testing/measurementSilica nanoparticlesSilicon dioxide
The invention provides a nucleic acid qualitative detection method based on nano-particles and rolling circle amplification. The detection method mainly comprises the steps of: allowing silica nanoparticles in a disperse state in a solution before the rolling circle amplification is used; performing the rolling circle amplification when a target nucleic acid molecule is present; and gathering the silica nanoparticles to form a macroscopic white blocky substance in the solution so as to achieve the purpose of qualitatively detecting the target nucleic acid molecule. As the presence of the target nucleic acid molecule can be judged by gathering states of the silica nanoparticles before and after rolling circle amplification, a large number of trace nucleic acid molecules are amplified, results are rapidly and intuitively detected without a special detection instrument, the detection cost is reduced, and the nucleic acid qualitative detection method is suitable for popularization of clinical detection.
Owner:PEKING UNIV
Method for colourimetric identifying detecting DNA molecule
InactiveCN1584050ARapid Qualitative DetectionRapid Quantitative DetectionMicrobiological testing/measurementBiologyDNA
A colorimetric identifying and detecting DNA method is disclosed. It has double functions of allochroic indicant group and reinforced allochroism by functional polymeric butadiyne vesiculation. It achieves simple and rapid process.
Owner:NORTHEAST NORMAL UNIVERSITY
VEGF (vascular endothelial growth factor) monoclonal antibody and fracture healing evaluation antibody chip with same
InactiveCN102863529AHigh potencyHigh-throughput detectionPeptide librariesImmunoglobulins against growth factorsDrug biological activityStandardization
The invention relates to the technical field of bioengineering and discloses a VEGF (vascular endothelial growth factor) monoclonal antibody and a fracture healing evaluation antibody chip with the same. The amino acid sequence of a heavy chain variable region of the monoclonal antibody is shown as SEQ ID NO.1, and the amino acid sequence of a light chain variable region of the monoclonal antibody is shown as SEQ ID NO.2. Homogeneity and biological activity unicity of the monoclonal antibody enables antigen antibody reaction results to be convenient for quality control and beneficial to standardization and normalization. The chip with the monoclonal antibody can be directly applied to clinical therapeutic evaluation on fracture healing, solves the technical problems of time and labor consumption, poor result repeatability and the like of the prior art, has extremely high application value in the field of clinical treatment and has wide application prospect.
Owner:李彬
Colloidal gold test strip for rapidly detecting double viruses of tobacco mosaic virus-potato virus Y (TMV-PVY)
InactiveCN106244561ALow costSimple and fast operationMicroorganism based processesImmunoglobulins against virusesBALB/cTobacco mosaic virus
The invention discloses a colloidal gold test strip for rapidly detecting double viruses of tobacco mosaic virus-potato virus Y (TMV-PVY). The test strip consists of a sample pad, a colloidal gold pad, an NC film, a piece of water absorption filter paper and a back lining, wherein the colloidal gold pad is enveloped with a TMV specific antibody and a PVY specific antibody which are marked by colloidal gold, and the TMV specific antibody and the PVY specific antibody are respectively generated by secretion by hybridoma cell lines, i.e., BALB / c-15-50 and BALB / c-15-8; the NC film is provided with a detection line and a control line; the detection line is enveloped with specific antigens of the TMV and the PVY; the control line is enveloped with a second antibody marked by the colloidal gold. The rapid detection colloidal gold test strip is especially suitable for the detection on the TMV and the PVY in Guangdong tobacco-growing areas, and is rapid, sensitive and accurate in dection, low in cost and simple and convenient in operation; the test strip can be used to diagnose the two viruses at the same time by one-time sampling, thus being very suitable for field primary screening of a great batch of samples; therefore, the colloidal gold test strip has a good application value in actual production and is good in popularization and application prospects.
Owner:SOUTH CHINA AGRI UNIV
Mercury ion colloidal gold colorimetric detection method and mercury ion detection kit
InactiveCN103901030ARapid Qualitative DetectionNo surface modification requiredMaterial analysis by observing effect on chemical indicatorMercuric ionCitric acid
The invention discloses a mercury ion colloidal gold colorimetric detection method and a mercury ion detection kit. According to the mercury ion colloidal gold colorimetric detection method, mercury ions are adsorbed to a gold nano particle solution (colloidal gold) wrapped with citric acid ions, and then lysine capable of specifically identifying the mercury ions is added to form a bridge, so that gold nano particles are gathered, and the mercury ions can be quickly detected on the spot according to the color change. The lysine serving as an important reagent is low in cost and readily available, so that the cost is greatly reduced; furthermore, the surfaces of the gold nano particles are not required to be modified, so that the method is convenient and quick. The mercury ion colloidal gold colorimetric detection method has the advantages of quickness, high sensitivity, high specificity, high stability, low cost and the like and is easy to popularize and conventionally apply.
Owner:HEFEI UNIV OF TECH
Nucleic acid aptamer of cortisol, and gold-colloidal test strip used for detecting cortisol
InactiveCN107356740ARapid Qualitative DetectionReduced Quantitative DetectionMaterial analysisAptamerBiotin-streptavidin complex
The invention discloses a cortisol nucleic acid aptamer and a gold standard test strip for detecting cortisol, which belong to the technical field of hormone detection. The sequence of the nucleic acid aptamer of the present invention is shown in SEQ ID NO.1, and the sequence of its complementary probe A is shown in SEQ ID NO.2. The gold standard test strip of the invention comprises a sample glass fiber membrane, a nucleic acid probe gold standard pad, a nitrocellulose membrane, a water-absorbing glass fiber membrane and a plastic liner. The nucleic acid aptamer of cortisol labeled with colloidal gold is attached to the nucleic acid probe gold standard pad; the detection line and the reference line are drawn on the nitrocellulose membrane, the detection line is drawn by the cortisol-bovine serum albumin conjugate solution, and the reference line is drawn by A solution of biotin-modified complementary probe A conjugated with streptavidin was drawn. The invention does not need other auxiliary equipment, can complete the detection of cortisol within 10 to 15 minutes, and the result is accurate and reliable; the sensitivity and specificity are high, the stability is good, the cost is low, and the application range is wide.
Owner:华测康诺(武汉)生物科技有限公司
Method for detecting lime substances in flour by near infrared spectroscopy
InactiveCN102213674ARapid Qualitative DetectionThe method steps are simpleColor/spectral properties measurementsQuantitative determinationPollution
The invention provides a method for detecting lime substances in flour by near infrared spectroscopy, which comprises the following steps of: acquiring a near infrared spectrum of a flour sample to be detected; determining whether the flour sample to be detected contains the lime substances or not according to the near infrared spectrum characteristics of the lime substances and pure flour; if a determined result is that the flour sample to be detected contains lime substances, acquiring a near infrared spectrum of a standard series flour sample; establishing a quantitative correction model according to the near infrared spectrum of the standard series flour sample; and detecting the content of the lime substances in the flour sample to be detected according to the quantitative correction model. The method for detecting the lime substances in the flour by the near infrared spectroscopy is simple in steps and pollution-free, and can be used for detecting whether the flour contains the lime substances or not quickly and qualitatively; and multiple lime substances in the flour can be measured quickly and quantitatively by combining the near infrared spectrum of the standard series flour sample and the quantitative correction model, and the obtained results are accurate and reliable.
Owner:BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
A method for detecting telomerase activity
ActiveCN103529023BRapid Qualitative DetectionHighly sensitive activity quantitative detectionMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementTelomeraseHybridization reaction
Owner:SOUTHEAST UNIV
Integrated totally-closed detection reaction tube
PendingCN113278492ASimple designEasy to processBioreactor/fermenter combinationsHeating or cooling apparatusBacterial virusEngineering
The invention provides an integrated totally-closed detection reaction tube. Specifically, the detection reaction tube comprises: an upper body, wherein the upper body is provided with a cover, a first cavity and a second cavity, the second cavity is located below the first cavity, the first cavity and the second cavity are provided with a fluid channel, and the fluid channel allows fluid to flow into the second cavity from the first cavity; and a lower body, and the lower body is provided with a detection cavity and a puncture component; The upper body and the lower body are hermetically connected together through a coupling structure. In addition, the invention further provides a corresponding detection method, a detection device and application. By means of the integrated totally-closed detection reaction tube, qualitative detection on bacteria and viruses containing target sequences and other nucleic acid-containing samples can be carried out quickly, conveniently and efficiently.
Owner:上海鲸舟基因科技有限公司
Mabuterol colloidal gold test strip and preparation method thereof
InactiveCN103149352ARapid Qualitative DetectionThe result is accurateSerum albuminImmunoglobulinsAntigenColloid
The invention discloses a mabuterol colloidal gold test strip and a preparation method thereof. The mabuterol colloidal gold test strip comprises a base plate, wherein the base plate is provided with a first end and a second end, and a sample cushion, a combiner cushion, a nitrocellulose membrane and a water sucking cushion are arranged on the base plate in sequence along the direction the first end to the second end. An anti-mabuterol monoclonal antibody which is marked by colloidal gold is contained on the combiner cushion. A detecting line and a quality control line are further formed on the nitrocellulose membrane, wherein the detecting line is composed of mabuterol detecting antigen threadiness sample application which can be combined with the anti-mabuterol monoclonal antibody. The quality control line is composed of ass anti-mouse antibody sample application which can be combined with the anti-mabuterol monoclonal antibody. The mabuterol colloidal gold test strip can be used for effectively detecting Mabuterol.
Owner:江西中德生物工程股份有限公司
Device and method for rapidly detecting residual quantity of abamectin B2a in soil
ActiveCN111751536AIncrease contact rateImprove extraction efficiencyBiological material analysisSoil scienceAgricultural engineering
The invention discloses a device and a method for rapidly detecting the residual quantity of abamectin B2a in soil. The device comprises a preprocessing unit, a detection unit and a control unit; thepreprocessing unit comprises a preprocessing box body, a roller shaft used for crushing samples in a sampling bag and a beaker used for extraction; the detection unit comprises a detection box body and a detection area located on one side of the detection box body and connected with the beaker through a sample feeding pipe; a detection test paper assembly is placed in the detection area, and a collection bottle used for collecting waste liquid is arranged below the detection area. According to the invention, the test paper is used for detecting the sample liquid;, a plurality of the detectionbelts are arranged, such that the rapid qualitative detection of the residual quantity of abamectin B2a can be achieved, the semi-quantitative detection can be performed, the detection range is large,and detection personnel can be assisted to determine the soil pollution condition in the region; and the device has the advantages of convenient detection, high detection accuracy and the like.
Owner:NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
TMV single colloidal gold quick detection test paper strip
PendingCN106497887ALow costSimple and fast operationImmunoglobulins against virusesTissue cultureBALB/cTobacco mosaic virus
The invention discloses a tobacco mosaic virus (TMV) single colloidal gold quick detection test paper strip, which is composed of a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a back lining. The colloidal gold pad is coated by a TMV specific antibody marked by colloidal gold. The antibody is secreted by hybridoma cell strain BALB / c-15-50; a detection line and a control line are arranged on the NC membrane, wherein the detection line is coated with specific antigens of three viruses, and the control line is coated with a secondary antibody marked by the colloidal gold. The test paper strip is mainly used for detection on the TMV in tobacco planting regions in Guangdong, is quick, sensitive, accurate and low-cost, has simple operations and achieves simultaneous diagnosis of multiple viruses through one-time sample collection, and is very suitable for in-site primary screening of a large amount of samples. The test paper strip has great application value in practical production and has good promotion and application prospects.
Owner:SOUTH CHINA AGRI UNIV
Method and device for biological sample detection
ActiveCN101526520BReduce consumptionShorten the timeMicrobiological testing/measurementBiological testingControl systemEngineering
The invention provides a method and a device for the synchronous and rapid high-throughput and multiple-information detection of multiple indexes of various samples under a simple and improvised condition. The device adopted in the method belongs to a microflow control system, the width of a notch on the cross section of a groove is 0.01-5000 micrometers, and the vertical height from the notch tothe minimum point of the groove is 0.01-5000 micrometers, so that the entire detection device can perform information collection of various biological matters to the various biological liquid samplesor treated exsomatized solution in a 1 cm square space. The invention successfully takes the non-specific adsorption as the judging basis of the experimental result, saves the consumption of the reagent and the samples when rapidly finishing the experimental operation, retains the activity of the samples and the reagent, omits the steps of surface closing and cleaning in the prior detection method, reduces the complexity and the condition limitation of the experiment and both has the advantages of the immunoblotting and the enzyme-linked immunity.
Owner:BEIJING NANO ACE TECH CO LTD
Method for detecting metabolites of protopanaxadiol inside human body
InactiveCN106918652AHigh resolutionHPLC tandem high resolutionComponent separationMetaboliteProtopanaxadiol
The invention relates to a method for detecting in-vivo metabolites of protopanaxadiol in blood plasma or urine samples, belonging to the field of pharmacoanalysis. According to the method, an ultra-high performance liquid chromatograph Agilent 1290 and a high-resolution mass spectrometer AB 5600+Q TOF are cooperatively used; an electrospray ionization (ESI) source is employed; a chromatographic column used in the invention is Agilent Eclipse plus C18RRHD with a filler particle size of 1.8 [mu]m, an inner diameter of 2.1 mm and a length of 50 mm; a mobile phase consisting of a 0.5% aqueous formic acid solution (A) and acetonitrile (B) is employed for gradient elution; flow velocity is 0.3 mL / min; column temperature is 30 DEG C; the elution procedures of gradient elution with the mobile phase comprise elution with 95% A in a time period of 0 to 1 min, elution with 95-75% A in a time period of 1 to 1.5 min, elution with 75-25% A in a time period of 1.5 to 6.0 min, elution with 25-0% A in a time period of 6.0 to 12.0 min, elution with 0% A in a time period of 12.0 to 13.0 min and elution with 0-95% A in a time period of 13.0 to 15.0 min; and qualitative detection is carried out the metabolites of protopanaxadiol inside the human body, and unknown metabolites are identified through comparison with blank samples. The method provided by the invention has the advantages of high speed, sensitivity, high resolution, high separation degree, etc.
Owner:FUDAN UNIV
Detection method for rapidly detecting beta-stimulant and paper-based immunosensor
PendingCN113237875AHigh sensitivityImprove stabilityMaterial analysis by observing effect on chemical indicatorCelluloseBiochemical engineering
The invention discloses a detection method for rapidly detecting beta-stimulant and a paper-based immunosensor. The manufacturing method of the paper-based immunosensor comprises the steps of: S1, designing a pore plate model through drawing software, then, printing the pore plate model designed previously on filter paper rich in cellulose through hydrophobic wax by means of a color printer, placing the filter paper printed with the pore plate model on a magnetic heater, heating for 2 minutes, and cooling to room temperature to obtain a paper-based micro-pore plate; and S2, adding beta-stimulant coating antigen into each circular reaction area of the paper-based micro-pore plate, drying the beta-stimulant coating antigen, then adding BSA confining liquid into each circular reaction area of the paper-based micro-pore plate, and drying the BSA confining liquid to obtain the paper-based sensor. According to the invention, the convenient paper-based immunosensor is utilized, so that the beta-stimulant can be quickly and sensitively detected in a colorimetric manner, and powerful technical support is provided for field detection, supervision and the like of the beta-stimulant.
Owner:安徽省产品质量监督检验研究院
Method for detecting permanganate radicals by coumarin-based probe
ActiveCN112945925AQuick responseStrong signalGeneral water supply conservationFluorescence/phosphorescenceDouble bondKetone
The invention provides a method for detecting permanganate radicals by a coumarin-based probe. The coumarin-based probe is 2-oxo-2hydrogen-benzopyran-7-R acid ester, the coumarin-based probe is obtained by condensation of 7-hydroxybenzopyran-2-ketone and acyl chloride, and the response group of the probe to the permanganate radicals is double bonds. The single probe has no fluorescence in a mixed solution of an organic solvent and water, and shows obvious fluorescence lightening response when being used for detecting the permanganate radicals, the response time is less than 3 seconds, and real-time detection of the permanganate radicals can be realized; and the detection sensitivity is high, and the detection limit is as low as 0.95 nmol / L. The method has high selectivity on the permanganate radicals, and the content of the permanganate radicals in a water sample and a non-standard explosive raw material in the environment can be accurately measured by utilizing a standard curve. The method has excellent selectivity and sensitivity, can be used for rapidly detecting the permanganate radicals on site, and has a wide application prospect.
Owner:XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
Method applying AlphaLISA to detect dengue virus and corresponding combination and kit
InactiveCN109444414AImprove featuresHigh sensitivityMaterial analysisAgainst vector-borne diseasesBiotin-streptavidin complexAntigen
The invention provides a method applying AlphaLISA to detect a dengue virus and corresponding combination and kit. An AlphaLISA detection composition comprises receptor bead coated with a capture antibody, a detection antibody marked with biotin and donor bead coated with streptavidin, and the capture antibody and the detection antibody are a pair of matched antibodies resistant to dengue virus NS1 protein. The composition serves as antigen for the NS1 protein, thereby having high specificity and sensitivity. The invention further discloses an AlphaLISA kit for detecting the dengue virus. Thekit comprises the AlphaLISA detection composition, a 384-hole microporous plate, a positive quality control product and a negative quality control product, the positive quality control product is matrix serum of the dengue virus NS1 protein, and the negative quality control product is a solution containing basic buffer. The microporous plate with many holes can be used for detection, and quick andqualitative detection of the dengue virus in high-throughput large samples can be realized.
Owner:珠海国际旅行卫生保健中心
Gold-labeled strip based on nucleic acid aptamers of progesterone in saliva and used for detection
InactiveCN105647930ARapid Qualitative DetectionReduced Quantitative DetectionBiological material analysisBiological testingAptamerLap joint
The invention discloses a gold-labeled strip based on nucleic acid aptamers of progesterone in saliva and used for detection. The 5' end of a sequence of the nucleic acid aptamers of progesterone is modified by sulfydryl, the 5' end of a complementary probe A sequence is modified by biotin, and the 5' end of a complementary probe B sequence is modified by biotin. According to the gold-labeled strip for detecting progesterone, a sample glass fiber membrane, a nucleic acid probe gold-labeled cushion, a nitrocellulose membrane and a water absorption glass fiber membrane are sequentially arranged on a plastic substrate in a lap joint mode, a detection line and a reference line are drawn on the nitrocellulose membrane, and the reference line is close to one end of the water absorption glass fiber membrane. Colloidal gold with the surface modified by the nucleic acid aptamers is attached to the nucleic acid probe gold-labeled cushion, the detection line is drawn with a nucleic acid aptamer A solution, and the reference line is drawn with a nucleic acid aptamer B solution. No other auxiliary instrument or equipment is needed, detection can be completed within 10-15 min, and the result is accurate and reliable; sensitivity and specificity are high, stability is good, cost is low, and the application range is wide.
Owner:李斌
Terahertz spectrum qualitative detection method for student school uniform textile material
ActiveCN113092403ARapid Qualitative DetectionQualitative detection is accuratePreparing sample for investigationColor/spectral properties measurementsTime domainEngineering
The invention discloses a terahertz spectrum qualitative detection method for a student school uniform textile material, which comprises the following steps: preparing different textile material experiment samples of a student school uniform, obtaining terahertz absorbance spectrums of different textile materials by using a terahertz time-domain spectroscopy system, and extracting feature information of a grey-scale map of the terahertz absorbance spectrums by using a Krawtchouk moment; combining an improved particle swarm algorithm with a support vector machine to establish a qualitative model, obtaining a classification identification model of different textile materials; collecting the textile materials on the school uniform, obtaining the feature information of the textile materials on the school uniform, and sending the feature information of the textile materials on the school uniform into the classification identification model of the corresponding textile materials for qualitative detection. The terahertz spectrum can be used for carrying out sexual detection on the textile material of the school uniform, so that the school uniform quality of primary and secondary school students is ensured, and primary and secondary school student safety incidents caused by the school uniform quality are avoided.
Owner:GUILIN UNIV OF ELECTRONIC TECH
PVY single virus colloidal gold rapid test strip
The invention discloses a PVY single virus colloidal gold rapid test strip which is composed of a sample pad, a colloidal gold pad, an NC membrane, water absorption filter paper and a backing; the colloidal gold pad is coated with a colloidal gold-labeled specific PVY antibody, and the antibody is secreted from a hybridoma cell strain BALBc-15-8; the NC membrane is provided with a test line and a control line, the test line is coated with a specific PVY antigen, and the control line is coated with a colloidal gold-labeled secondary antibody. The test strip is rapid to operate, sensitive, accurate, low in cost and easy and convenient to operate for testing PVY in Guangdong tobacco-growing areas, achieves simultaneous multi-sample diagnosis in one-time sampling, is very suitable for conducting site primary screening on a large scale of samples and has an application value in practical production and a good application and popularization prospect.
Owner:SOUTH CHINA AGRI UNIV
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