5results about How to "No false positives" patented technology

The present invention discloses a nucleic acid aptamer of uridine diphosphate and a screening method and application thereof, wherein the sequence of the nucleic acid aptamer is shown in SEQ ID NO: 1; the screening method is CIVT-SELEX, i.e., the Capture-SELEX method is first used for primary screening to obtain a single-stranded nucleic acid that can bind to a target ligand, and then the in vitro transcription process is used for screening again, thereby obtaining a nucleic acid aptamer that undergoes a significant conformational change after binding to the target ligand. The present invention successfully obtains a nucleic acid aptamer sequence that can detect uridine diphosphate in an enzymatic reaction solution in a single-stranded linear form, and can respond to different concentrations of uridine diphosphate during in vitro transcription to activate and regulate downstream gene transcription; CIVT-SELEX is expected to be applied to the screening of aptamer sequences of other target ligands, and can be used for detection and as a gene element in a gene circuit for regulation.

The utility model discloses a kind of anti-disassembly devices of GPS positioning equipment, including device shell, integrated circuit board is also set in the device shell, the integrated circuit board is installed in device shell by positioning structure, magnet mounting structure is also assembled and set in the device shell, the magnet mounting structure is connected with the object to be installed;The magnet mounting structure includes installation box, magnet block is set in the installation box, the installation box is set in the middle part of device shell;Integrated circuit board side is also integratedly arranged Hall sensor, the magnetic field of Hall sensor is perpendicular with magnet block arrangement, GPS locator is also set on integrated circuit board, Hall sensor used in the application is not influenced by ambient light, can work stably in night, tunnel and other low-light environment, also will not cause false alarm due to sunlight direct incidence or strong light irradiation, and can be installed quickly, simultaneously magnet mounting structure can be hidden installation, improve anti-disassembly effect.

The invention discloses a molecular marker closely linked with allelic variation Nic1-5 of a major regulation and control site for tobacco nicotine synthesis and application of the molecular marker. The molecular marker comprises NANL-a, NANL-b, NANL-c, NANL-d and NANL-e, and the nucleotide sequences of the NANL-a, the NANL-b, the NANL-c, the NANL-d and the NANL-e are respectively shown as SEQ ID NO.1-5. The invention also provides a specific primer for amplifying the molecular marker, and the nucleotide sequence of the specific primer is as shown in SEQ ID NO.6-15. By utilizing the molecular marker and the primer, Nic1-5 allelic variation can be rapidly and accurately detected, a normal nicotine genotype and a low nicotine genotype and characters are distinguished, and molecular marker-assisted selective breeding is realized. The invention provides a non-transgenic molecular detection tool for breeding the low-nicotine tobacco variety, has the advantages of strong specificity, high accuracy, simplicity and convenience in operation and the like, and can obviously improve the breeding efficiency and accelerate the breeding process of the low-nicotine tobacco variety.

The application discloses a method for early detection of turn-to-turn short circuit of a stator winding of an induction motor based on a voltage signal, and comprises the following steps: collecting three-phase stator winding voltage signals of an induction motor to be detected during operation; performing time-frequency analysis to obtain time-frequency energy; constructing a cost function, and initializing a time-frequency ridge line of the voltage signal; updating the time-frequency ridge line to realize accurate estimation of the time-frequency ridge line of the voltage signal; calculating three-phase voltage symmetrical components, performing time-varying band-pass filtering, and obtaining fundamental waves of the three-phase symmetrical components; calculating amplitudes of the three-phase symmetrical component fundamental waves, constructing an extended zero-sequence voltage factor, and realizing real-time detection of a turn-to-turn short circuit fault; and constructing a fault phase index to realize identification of a turn-to-turn short circuit phase. The application can effectively detect an early turn-to-turn short circuit fault, improves the accuracy of online detection, reduces the hardware cost of online detection, improves the robustness of online detection under non-stationary conditions, reduces the complexity of data acquisition and signal processing, and improves the practicability of intelligent health management.

The present invention provides a multiplex PCR primer set for detecting whether a Turner syndrome patient carries Y chromosome material and its application, belonging to the field of biological detection technology. The nucleotide sequence of the multiplex PCR primer set for detecting whether a Turner syndrome patient carries Y chromosome material provided by the present invention is shown as SEQ ID NO.1-SEQ ID NO.18. Using the primer set provided by the present invention to detect whether a Turner syndrome patient carries Y chromosome material has the advantages of low detection cost, high efficiency, simple operation and easy promotion, strong specificity, high detection sensitivity, and short detection cycle. It can screen Y chromosome material before growth hormone treatment of Turner syndrome patients, and provide a diagnostic basis for whether Turner syndrome patients need preventive gonadectomy.