2results about How to "No false positives" patented technology

The invention discloses a molecular marker closely linked with allelic variation Nic1-5 of a major regulation and control site for tobacco nicotine synthesis and application of the molecular marker. The molecular marker comprises NANL-a, NANL-b, NANL-c, NANL-d and NANL-e, and the nucleotide sequences of the NANL-a, the NANL-b, the NANL-c, the NANL-d and the NANL-e are respectively shown as SEQ ID NO.1-5. The invention also provides a specific primer for amplifying the molecular marker, and the nucleotide sequence of the specific primer is as shown in SEQ ID NO.6-15. By utilizing the molecular marker and the primer, Nic1-5 allelic variation can be rapidly and accurately detected, a normal nicotine genotype and a low nicotine genotype and characters are distinguished, and molecular marker-assisted selective breeding is realized. The invention provides a non-transgenic molecular detection tool for breeding the low-nicotine tobacco variety, has the advantages of strong specificity, high accuracy, simplicity and convenience in operation and the like, and can obviously improve the breeding efficiency and accelerate the breeding process of the low-nicotine tobacco variety.

The application discloses a method for early detection of turn-to-turn short circuit of a stator winding of an induction motor based on a voltage signal, and comprises the following steps: collecting three-phase stator winding voltage signals of an induction motor to be detected during operation; performing time-frequency analysis to obtain time-frequency energy; constructing a cost function, and initializing a time-frequency ridge line of the voltage signal; updating the time-frequency ridge line to realize accurate estimation of the time-frequency ridge line of the voltage signal; calculating three-phase voltage symmetrical components, performing time-varying band-pass filtering, and obtaining fundamental waves of the three-phase symmetrical components; calculating amplitudes of the three-phase symmetrical component fundamental waves, constructing an extended zero-sequence voltage factor, and realizing real-time detection of a turn-to-turn short circuit fault; and constructing a fault phase index to realize identification of a turn-to-turn short circuit phase. The application can effectively detect an early turn-to-turn short circuit fault, improves the accuracy of online detection, reduces the hardware cost of online detection, improves the robustness of online detection under non-stationary conditions, reduces the complexity of data acquisition and signal processing, and improves the practicability of intelligent health management.