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Chemiluminescent ligand analysis method for quantitative detection of human auto-antibody

An autoantibody and chemiluminescence technology, applied in the field of biomedicine, can solve the problems of inability to provide a reliable basis for autoimmune disease diagnosis and efficacy evaluation, and inability to accurately quantify autoantibodies, achieving high sensitivity, accuracy and specificity. Effect

Inactive Publication Date: 2009-07-01
天津市协和医药科技集团有限公司
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Problems solved by technology

This method cannot accurately quantify autoantibodies, so it cannot provide a reliable basis for the diagnosis and efficacy evaluation of autoimmune diseases

Method used

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  • Chemiluminescent ligand analysis method for quantitative detection of human auto-antibody
  • Chemiluminescent ligand analysis method for quantitative detection of human auto-antibody

Examples

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Embodiment Construction

[0041] The content of this method is specified by the following examples:

[0042] Establishment of Quantitative Analysis Method for TPO Autoantibody Chemiluminescent Ligand (Nanomagnetic Particles)

[0043] (1) Preparation of each component of the kit:

[0044] The main components of TPO-Ab chemiluminescence ligand quantitative analysis diagnostic kit are: marker (TPO-HRP), standard (TPO monoclonal antibody), ligand reagent (SPA-nano magnetic particles), enhanced chemiluminescence working solution ,detergent.

[0045] 1. Preparation and detection of labeled antigen (TPO-HRP):

[0046] a. Preparation: TPO-HRP was prepared by sodium periodate enzymatic labeling technique:

[0047] (1) Weigh 2.0mg of TPO into a 5ml glass bottle, add 1.0ml (0.05mol / L, pH7.5) carbonate buffer to dissolve, and store at 4°C for later use;

[0048] (2) Weigh 10.0mg of HRP into a 16×100mm glass test tube, add 1.0ml of deionized water, oscillate, after it is completely dissolved, take out 0.74ml an...

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Abstract

A chemical luminous ligand analysis method for quantitatively checking human antibodies belongs to the biomedical technical field, which comprises: using the generality that staphylococcal protein A (SPA) can react with Fc point of IgG in mice; using the monoclonal antibody of high affinity and specificity to prepare a standard product to establish a standard curve; respectively reacting the monoclonal antibody and the antibody in the sample with enzyme-labeled antigens; using the nanometer magnetic particles coated by SPA as ligand to separate solid and liquid; and using enzymatic chemical illumination reaction catalyzed by horseradish peroxidase (HRP) to process illumination check; thereby establishing a chemical luminous ligand quantitative analysis on human antibodies. The chemical luminous ligand analysis method is suitable for quantitatively checking all human antibodies, for resolving the problem of prior art while most human antibodies can not be accurately and quantitatively checked, avoiding cross reaction and avoiding false negative result or false positive result.

Description

Technical field: [0001] The invention relates to the technical field of biomedicine, and relates to a quantitative analysis method for chemiluminescent ligands (nano magnetic particles) for quantitative detection of autoantibodies in human body in vitro. Background technique: [0002] Autoimmune diseases refer to a large class of diseases caused by the body's immune response to self-antigens, resulting in damage to self-tissues and organs and corresponding dysfunction. There are at least 30 kinds of autoimmune diseases that have been recognized at present. The growth, development and survival of the human body are maintained by a complete autoimmune tolerance mechanism, and the normal immune response has a protective defense function, that is, no immune response occurs to its own tissues and components. Once the integrity of self-tolerance is destroyed, the body regards its own tissues and components as "foreign bodies", thus autoimmune reactions occur and autoantibodies ar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/58G01N33/577
Inventor 王立凯潘学继
Owner 天津市协和医药科技集团有限公司
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