118 results about "Chemiluminescence response" patented technology

The present invention provides a novel chemiluminescent reagent producing chemiluminescence in the presence of hydrogen peroxide, extent of which depends on peroxidase concentration, chemiluminescent analysis method using the same, in particular useful for detection and quantitative analysis of various types of materials by measuring peroxidase enzyme activity or enzyme immunoassay with peroxidase enzyme as the marker.More particularly, the present invention provides a chemiluminescent reagent containing, as the major ingredients, a charge-transferring complex of N,N'-disubstituted-9,9'-bisacridinium salt and N,N-disubstituted carboxylic amide compound; chemiluminescent reagent containing further containing a specific aminoalcohol compound, in addition to the above; and method for measuring peroxidase activity at a high sensitivity in the presence of a peroxide, using the above chemiluminescent reagent.Moreover, the novel chemiluminescent reagent of the present invention can enhance sensitivity of the enzyme immunoassay with peroxidase enzyme as the marker by its chemiluminescent reaction.

The invention discloses a micro-reactor suitable for micro-liquid mixing and biochemical reaction and a manufacturing method thereof. The micro-reactor comprises an upper cover plate and a lower base plate which is butted with the upper cover plate. A piezoelectric film is arranged between the lower base plate and the upper cover plate. The piezoelectric film is provided with a micro channel, an outlet and an inlet of which are respectively corresponding to a liquid outlet and a liquid inlet of the upper cover plate. A surface acoustic wave generator is also arranged between the lower base plate and the piezoelectric film. According to the invention, a temperature control system is integrated into the micro-reactor such that a reaction in the micro-reactor can be conducted under the condition of constant temperature. By the mechanism of surface acoustic wave, mechanical vibration can be generated to drive non-charged liquids to be mixed, and an alternating electric field also will be generated on an energy transducer. Thus, the micro-reactor is endowed with a liquid vibration function by surface acoustic wave, a liquid driving function by the alternating electric field and a microenvironment temperature control function, and efficiency of a micro-liquid chemiluminiscence reaction can be raised greatly. Therefore, the structure also has a wider application range, and will have a more obvious effect of driving charged liquids.

Assay methods are disclosed involving specific binding reactions which are simplified compared to known methods. A compound capable of producing chemiluminescence is immobilized on a solid support as is a member of a specific binding pair for capturing an analyte from a sample. An activator compound that activates the chemiluminescent compound and is conjugated to a specific binding pair member is added in excess along with the sample to the solid support. Addition of a trigger solution causes a chemiluminescent reaction at the sites where the activator conjugate has been specifically bound. The assay methods are termed non-separation assays because they do not require removal or separation of excess detection label (activator conjugate) prior to the detection step. The methods are applicable to various types of assays including immunoassays, receptor-ligand assays and nucleic acid hybridization assays.

A ballistic tracer platform for use with a shotgun shell to provide an aiming and training aid for shotgun shooting sports, which also can be used for military and police applications. The ballistic tracer platform emits light after ignition of the shell, providing the shooter with a consistent reference to make corrections to his aiming point and shooting techniques. The tracer platform can be used in ordinary shotgun shells. The tracer platform comprises a translucent, resilient, elastic, cylindrical container in which the reactants, a fluorescent colored dye and oxalate solution and an activator, are held, separated from each other prior to ignition by encasing one or both in its own glass bulb or tube. The blast from ignition of the shell causes the glass bulb(s) or tube(s) to break. The resulting chemiluminescent reaction between the reactants results in emission of light which is visible to the shooter.

The invention discloses a photoinduced electrochemical biological sensor with advantages of simple operation and high flux, which is successfully applied to simultaneously detect three types of cancer cells. The photoinduced electrochemical biological sensor is characterized in that a layer of gold nanoparticles is modified onto the surface of paper fiber through gold nanometer self-catalytic reduction; then, preparing cadmium sulfide-graphene-zinc oxide bar ternary composite materials at the surface of a paper working electrode which are modified by long gold, and using as photo-electrodes; using chemical light emitting to replace the traditional xenon lamp; by controlling the chemical light emitting reaction sequence, sequentially exciting photo-electrodes; sequentially detecting three photo-current peaks, so as to simultaneously detect the three types of cancer cells.

The invention provides a chemiluminescence reaction-based methylase detection probe, a detection kit and a detection method. The chemiluminescence reaction-based methylase detection probe provided by the invention is simple in design without being any labeled and modified. Meanwhile, according to the chemiluminescence reaction-based methylase detection probe, methylase and specific incision enzyme depending on methylation are used as a combination, a detection probe with a hairpin structure can be cut apart by depending an interaction of the methylase and the specific incision enzyme so as to generate a primer signal, and thus a chemiluminescence signal is generated. Moreover, any other action of the methylase to hairpin structures can not be recognized by incision enzyme outside the combination, and thus no primer signal can be generated, and therefore, no chemiluminescence signal is generated so that the specificity of the chemiluminescence reaction-based methylase detection probe is very high. In addition, according to the technical scheme adopted by the invention, two advantages of high ligase catalysis efficiency and remarkably enhanced chemiluminescence signal are serially connected, so that the detection sensitivity is greatly improved. The chemiluminescence reaction-based methylase detection probe is wide in application value on aspects of early diagnosis for diseases and screening of drugs.

A method is provided for determining in a medium the presence or amount of an analyte which is capable of binding to a ligand partner to form a ligand complex, which method comprises: (a) contacting the medium with either: (i) a ligand partner conjugated with an enzyme being capable of catalysing a reaction, or one or more reactions in a cascade thereof, to produce hydrogen peroxide, or (ii) a ligand partner and either a competing analyte or an analog of said analyte, a competing analyte or analyte analog being capable of forming a ligand complex with the ligand partner and the competing analyte or analyte analog being conjugated with an enzyme capable of catalysing a reaction, or one or more reactions in a cascade thereof, to produce hydrogen peroxide, (b) optionally separating complexed and uncomplexed enzyme conjugates, (c) causing or allowing the reaction or cascade of reactions to occur to produce hydrogen peroxide by contacting the complexed or uncomplexed enzyme conjugate with a corresponding enzyme substrate; (d) contacting hydrogen peroxide with a substance capable of exhibiting chemiluminescence in the presence of hydrogen peroxide selected from the group consisting of acridinium compounds and analogs thereof having a conjugate base with a pKa less then 9 to generate a chemiluminescent reaction, and (e) detecting the occurrence of said chemiluminescent reaction to determine the presence or amount of the analyte. Kits for use in connection with the method are also disclosed.

A chemiluminescent tracer insert with decelerator for use with a shotgun shell to provide an aiming and training aid for shotgun shooting activities, including skeet, trap, sporting clays, hunting, law enforcement and military applications. The tracer insert can be used in shotgun shells of all gauges. The tracer insert comprises a translucent, resilient, elastic, cylindrical container in which the reactants, an oxalate and fluorescent-colored dye solution, and an activator encased in a glass vessel, are held. Ignition of the shell causes the glass vessel to break. The resulting chemiluminescent reaction causes emission of light visible to the shooter. Unattached ends of thin-flaps on the tracer insert extend outwardly when drag forces act upon them during flight, slowing the speed of the tracer insert, which makes it more visible to the shooter, thereby providing a consistent reference to enable a shooter to make corrections to his lead and/or shooting techniques.

A bait for fishing is disclosed wherein the bait is attachable to lines of various diameters. The attachment means is integral to the elongated body of the bait made of a resilient plastic material and comprises at one end a slot with successive internal perpendicular grooves of different dimensions for receiving and maintaining the line. The bait is hollow and produces light resulting from a chemiluminescent reaction of two components originally stored in two separate compartment.

A chemical luminous ligand analysis method for quantitatively checking human antibodies belongs to the biomedical technical field, which comprises: using the generality that staphylococcal protein A (SPA) can react with Fc point of IgG in mice; using the monoclonal antibody of high affinity and specificity to prepare a standard product to establish a standard curve; respectively reacting the monoclonal antibody and the antibody in the sample with enzyme-labeled antigens; using the nanometer magnetic particles coated by SPA as ligand to separate solid and liquid; and using enzymatic chemical illumination reaction catalyzed by horseradish peroxidase (HRP) to process illumination check; thereby establishing a chemical luminous ligand quantitative analysis on human antibodies. The chemical luminous ligand analysis method is suitable for quantitatively checking all human antibodies, for resolving the problem of prior art while most human antibodies can not be accurately and quantitatively checked, avoiding cross reaction and avoiding false negative result or false positive result.

The present invention discloses a non-pyrotechnic, light emitting projectile for marking and illuminating a target. The projectile generally contains a main body having frangible side walls, at least one frangible ampoule containing chemiluminescent reactant components, a substantially incompressible filler material positioned between the frangible side walls and ampoule, and a first and second end cap. The first end cap is designed to be traversable between a first position and a second position upon impact with an object, thus provides a force sufficient to rupture the frangible side walls and ampoules. Rupture of the ampoules causes intermixing of the chemiluminescent reactant components and formation of a chemiluminescent slurry. The second end cap provides sufficient energy transfer to the chemiluminescent slurry upon impact with a target to disperse the chemiluminescent slurry radially and outwardly with respect to the longitudinal axis of the main body.

The invention discloses a kit and a method for detecting hepatitis B virus surface antigen (HBsAg). The detection kit is used for marking hepatitis B virus surface antibody (HBsAb) by using metalloporphyrin obtained by using water-soluble A3B type metalloporphyrin to mark the HBsAb. The detection kit and the detection method have the advantages that: (1) free metalloporphyrin molecules are separated from a coupled substance easily after an A3B type metalloporphyrin complex is coupled with the HBsAb to obtain a high-purity labelled antibody, so that non-specific adsorption background signals are reduced when the HBsAg is detected by chemical luminescence immunodetection, and detection sensitivity is greatly improved; (2) the method is simple, convenient and quick; automation is realized easily; and specificity of immunoreaction, high sensitivity of chemical luminescence reaction and stability of a chemical marker are achieved; and (3) a new method is provided for detecting the HBsAg quickly and accurately, and preventing and treating the hepatitis B virus.

A formable, porous chemiluminescent reactant composition, device therefore, and a process for production thereof is disclosed. The fluidizable solid admixture of the instant invention may be cured to a more or less rigid form with or without the use of a mold. The cured solid is useful as a chemiluminescent reactant component and is useful in a variety of environments.

The invention relates to a construction method and an application of chemiluminiscence-colloidal gold immunochromatography test paper, which has high sensitivity and is quantitative. The method comprises the following steps: chemiluminiscence and a colloidal gold immunochromatography method are combined, namely the surface of colloidal gold is simultaneously coupled with a detection antibody and an enzyme to form a colloidal gold-enzyme-antibody compound; the colloidal gold-enzyme-antibody compound and a coated antibody are sequentially sprayed on a sample combination cushion and a film; an immunochromatography reaction is carried out and qualitative detection is realized based on color shades of the colloidal gold; quantitative detection is carried out based on the light intensity of an enzyme catalyzed chemiluminiscence reaction so as to construct the chemiluminiscence-colloidal gold immunochromatography test paper; the test paper is applied to the detection of biological macromolecules and micro-molecules. The chemiluminiscence-colloidal gold immunochromatography test paper can simultaneously realize quantitative, qualitative and high-sensitivity detection and is suitable for field detection and domestic individualized diagnosis.

The invention discloses a gas chromatograph and detection method for determining the total content of sulfides in natural gas. The gas chromatograph comprises a quartz capillary column, a hydrogen flame ionization detector, a temperature control unit and a sulfur chemiluminescence reactor which are successively connected, wherein the quartz capillary column is a fused silica capillary column with uncoated inner walls; and the gas chromatograph further comprises a processor which is connected with the sulfur chemiluminescence reactor and a display unit. The gas chromatograph and detection method provided by the invention eliminate influence of hydrocarbons on determination of sulfide content, are more accurate in testing and have a wider application scope.

The invention discloses a substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide. The substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide comprises the following steps: manufacturing a fabric-based micro fluid control chip; dropwise adding a chemiluminiscence base solution to a detection zone of the chip, then airing at the temperature of 2-8 DEG C in a dark place, and storing; dropwise adding a sample solution to a sample introducing zone of the chip, if a sample contains H2O2, triggering chemiluminiscence reaction, acquiring a luminescence image by virtue of CCD (Charge Coupled Device) digital imaging equipment, and processing the luminescence image by virtue of software Matlab and Image J. A chemiluminiscence system of the substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide has the advantages of good stability, high detection sensitivity, wide dynamic range and the like and can be used for directly or indirectly determining hydrogen peroxide, so that the substrate-prefixed fabric-based micro fluid control chemiluminiscence method for detecting hydrogen peroxide has extremely important research significance in the fields of environmental monitoring, food safety testing, biological chemistry, clinical disease diagnosis and the like.

HIV-1 p24 antigen acridine ester chemiluminescence immunity analyzing testing method pertains to the clinical blood testing analysis method technique field. The prior HIV-1 p24 antigen testing method has problems of low sensitivity, rigmarole operations and the like. The invention uses acridine ester series compounds to mark the HIV-1 p24 antibody, adopts a double antibody/antigen sandwich method to execute immunity combination between the p24 antigen-antibody; uses excitant hydrogen peroxide, nitric acid, Triton X-100, and sodium hydrate to excite acridine ester series compounds to execute chemiluminescence reaction; and tests the number of photon to execute a qualitative and/or quantitative analysis. The invention has good correlativity with the general ELISA testing method, which has a correlation coefficient of 0.951 at the instance of P no more than 0.01; has high sensitivity, wherein, the testing limit is 0.5pg/ml; has wide detection range in 5-6 magnitude order; and the invention also has advantages of short reaction time, easier operation and the like.

The invention relates to a device for rapid detection of total volatile organic compounds. The device comprises a plasma generator, an ozone generator and a catalytic luminescence sensing device, wherein the plasma generator is provided with a sample gas inlet and a sample gas outlet. The ozone generator is provided with an ozone outlet. The catalytic luminescence sensing device includes a chemiluminescent reaction chamber with a catalyst inside and a photodetector. The sample gas inlet and the ozone outlet are respectively communicated with the chemiluminescent reaction chamber, respectively.The photodetector detects a catalytic luminescence signal emitted from the chemiluminescent reaction chamber. The invention also relates to a method for rapid detection of the total volatile organiccompounds. The device for rapid detection of the total volatile organic compounds can meet the requirements of rapid, sensitive and simple detection of the TVOC (total volatile organic compounds).

The invention discloses a device and a method for rapidly detecting sevoflurane online. The device comprises a sampling unit and a cataluminescence sensing unit, wherein the sampling unit comprises a sampling head and a double-channel air pump, the sampling head is connected with an air inlet of a first channel of the double-channel air pump, and an air inlet of a second channel of the double-channel air pump is communicated with air; the cataluminescence sensing unit comprises a chemiluminescence reaction chamber, a temperature controller, an optical splitter and a detector; a heating element is arranged in the chemiluminescence reaction chamber, and nanometer strontium oxide is sintered on the surface of the heating element; the temperature controller is electrically connected with the heating element, and the optical splitter is arranged between the chemiluminescence reaction chamber and the detector, so that light emitted from the chemiluminescence reaction chamber passes through the optical splitter and then is received and detected by the detector. According to the device and the method, nanometer strontium oxide is adopted as a sensor element, has good selectivity for sevoflurane and can be directly used for rapidly analyzing sevoflurane in complex samples.

An illuminated skeet target comprises a substantially disc-shaped body that is suitable for launching via a trap or other launching device. The skeet target contains at least two reagents that undergo a chemiluminescent reaction and give off light when combined so that the skeet target can be illuminated and identified when ambient lighting conditions are insufficient.

The invention discloses a chemiluminescence immunoassay kit for Seneca valley virus nonstructural protein 3ABC antibody detection. The kit comprises a Seneca valley virus nonstructural protein 3ABC antigen coated chemiluminescence immunoassay plate, positive control serum, negative control serum, an enzyme-labeled antibody, a sample diluent, a chemiluminescence substrate solution, a luminescence enhancing agent and concentrated washing liquid, wherein the amino acid sequence of the Seneca valley virus nonstructural protein 3ABC is shown as SEQ ID NO.2. The kit provided by the invention uses nonstructural protein 3ABC antigen expressed by a prokaryotic expression system for coating a reaction plate; the antigen consumption is low; whether the Seneca valley virus exists in the pig serum or not can be efficiently detected; in addition, reaction with the swine vesicular disease viruses, hog cholera viruses and porcine circovirus type 2 cannot occur. An enzymatic chemiluminescence reactionsystem is used for judging the result; the detection sensitivity is improved. The kit provided by the invention has the advantages that the specificity is high; sensitivity and high efficiency are realized; good market prospects are realized.

Methods, devices and kit for analyzing a sample comprising 1,5-anhydroglucitol and a first analyte via one or more chemiluminescent reactions. Certain embodiments include measuring a first light response resulting from a first chemiluminescent reaction and measuring a second light response resulting from a second chemiluminescent reaction. Certain embodiments also include comparing the first light response to the second light response to determine a ratio of 1,5-anhydroglucitol and the first analyte.

The invention discloses a chemiluminescence immunoassay kit for Seneca valley virus structural protein VP1 antibody detection. The kit comprises a Seneca valley virus structural protein VP1 antigen coated chemiluminescence immunoassay plate, positive control serum, negative control serum, an enzyme-labeled antibody, chemiluminescence substrate liquid, a luminescence enhancing agent and concentrated washing liquid, wherein the amino acid sequence of the Seneca valley virus structural protein VP1 is shown as SEQ ID NO.2. The kit provided by the invention uses structural protein VP1 antigen expressed by a prokaryotic expression system; the protein yield is high; the antigen purity reaches 90 percent or higher. An enzymatic chemiluminescence reaction system is used for judging the result; thedetection sensitivity is improved. The kit provided by the invention has the advantages that the operation is simple; the detection time is short; the sensitivity and the specificity are high, and thelike. The kit can be used for Seneca valley virus detection and epidemiological investigation of a great number of samples, and has good market prospects.