The invention provides a
chemiluminescence reaction-based methylase detection probe, a detection kit and a detection method. The
chemiluminescence reaction-based methylase detection probe provided by the invention is simple in design without being any labeled and modified. Meanwhile, according to the
chemiluminescence reaction-based methylase detection probe, methylase and specific incision
enzyme depending on
methylation are used as a combination, a detection probe with a hairpin structure can be
cut apart by depending an interaction of the methylase and the specific incision
enzyme so as to generate a primer
signal, and thus a chemiluminescence
signal is generated. Moreover, any other action of the methylase to hairpin structures can not be recognized by incision
enzyme outside the combination, and thus no primer
signal can be generated, and therefore, no chemiluminescence signal is generated so that the specificity of the chemiluminescence reaction-based methylase detection probe is very high. In addition, according to the technical scheme adopted by the invention, two advantages of high ligase
catalysis efficiency and remarkably enhanced chemiluminescence signal are serially connected, so that the detection sensitivity is greatly improved. The chemiluminescence reaction-based methylase detection probe is wide in application value on aspects of early diagnosis for diseases and screening of drugs.