31 results about "Chemiluminescence response" patented technology

The invention relates to a method for predicting the concentration of a target substance based on an intelligent analysis chemiluminescence detection system, and the method comprises the steps: precisely collecting the light intensity time sequence data of the chemiluminescence detection system through a PMT, extracting multiple key features, importing the multiple key features into an LSTM model, employing the LSTM model to adapt to the chemiluminescence reaction time sequence characteristics, and predicting the concentration of the target substance. The feature and concentration mapping relation is accurately learned, and the low-concentration sample prediction precision is improved; meanwhile, the model output contains confidence, the result reliability is quantified, and the misjudgment risk is reduced.

The present disclosure relates to a chemiluminescence analyzer, comprising: a sample preparation device configured to pre-dilute a blood sample under test by using a diluent, and mix the pre-diluted blood sample under test and a reaction reagent in a reaction vessel to obtain a sample liquid under test; a substrate providing device configured to provide a luminescent substrate into the reaction vessel containing the sample liquid under test so as to carry out a chemiluminescence reaction; a photometric device, at least comprising a photometer; and a data processing device configured to obtain a test result of human chorionic gonadotropin (HCG) in the blood sample under test on the basis of a luminescent signal and a preset calibration model. A first linear test range of the photometer is [A1, A2] photon count / second, and in the first linear test range, the intensity of the luminescent signal generated in the chemiluminescence reaction is linearly related to the photon count output by the photometer, wherein A1 is less than or equal to 2000 and A2 is greater than or equal to 108. Therefore, the testing efficiency and accuracy of HCG can be improved without increasing the costs.

The invention particularly relates to a method for determining a reaction rate constant of tetravalent iron and organic pollutants by using a chemiluminescence method, and belongs to the technical field of water quality detection. According to the method, on the basis of a chemiluminescence reaction between Fe (IV) and naproxen (NAP), a target organic pollutant (TrOC) is introduced, the quenching effect of the target organic pollutant on a chemiluminescence signal is measured, and a competitive kinetic model between the chemiluminescence quenching capacity (CLQC) and a secondary reaction rate constant (kFe (IV), TrOC) of Fe (IV) on the target pollutant is constructed. According to the method, the concentration of Fe (IV) does not need to be monitored in real time, and the reaction activity between Fe (IV) and various pollutants can be directly and rapidly measured only by comparing the intensity change of the chemiluminescence signals when the target pollutants exist and do not exist.

An airborne silica detection system provides a chemiluminescence reaction for quantitative assessment of silica on an automated basis. A prefilter allows reaction to be sensitive to particle sizes relevant to chronic respiratory diseases.

The invention relates to a chemiluminescence analyzer, comprising: a sample preparation device for mixing a to-be-detected blood sample and a reaction reagent in a reaction container to obtain a to-be-detected sample liquid; the substrate providing device is used for providing a luminous substrate into a reaction container filled with a sample solution to be detected so as to carry out a chemiluminescence reaction; the light measuring device at least comprises a photometer and is used for detecting a light-emitting signal generated in the chemiluminescence reaction; and the data processing device is used for obtaining a detection result of the to-be-detected substance in the to-be-detected blood sample according to the light-emitting signal and a preset calibration model. The first linear detection range of the photometer is [A1, A2] photon counting / second, A1 is smaller than or equal to 2000, and A2 is larger than or equal to 108. The second linear detection range of the light-emitting substrate is [B1, B2] photon counting / second, B1 is smaller than or equal to 3000, and B2 is larger than or equal to 108. According to the embodiment of the invention, the chemiluminescence analyzer with high sensitivity and high linear detection upper limit can be obtained.

This invention relates to the field of microfluidic sensing and detection technology, and more particularly to a photoelectric / fluorescence dual-mode self-calibrating microfluidic sensor for the detection of various mycotoxins, its fabrication method, and its application. The microfluidic sensor comprises a three-channel microfluidic chip and a substrate. In its fabrication, the three independent working electrodes and an integrated reference-counter electrode system are integrated into the microfluidic chip, utilizing oxygen-rich vacancy Io. 0.2~0.4 S 0.3~0.5 Using BiOBr (ISBO-OVs)@Au composite material as the sensing substrate, an internal secondary light source is formed by the chemiluminescence reaction of luminol-superoxide radicals, which can cascade and amplify the photocurrent signal. At the same time, combined with the specific recognition function of 7DCCA labeled aptamers, the sensitivity and specificity of microfluidic sensors for detecting trace fungal toxins are greatly improved.

This invention provides a photodiode current detection device and method with charge enrichment, enabling real-time acquisition and detection of both strong and weak signals. For strong signals, the real-time photocurrent signal can be directly acquired; for weak signals, the photodiode junction capacitance is used as a charge storage device. By exposing the photodiode to weak light for a period of time, the photocurrent will integrate and accumulate on the junction capacitance, resulting in a stronger accumulated signal, which is then measured, thus achieving weak signal detection. Applying this detection device and method to the chemiluminescence reaction principle for NO gas detection, the charge enrichment mode for detecting low-concentration NO gas yields a high signal-to-noise ratio signal, improving sensitivity. The direct measurement mode for detecting high-concentration NO gas does not affect the upper concentration limit of NO detection. The combination of these two operating modes significantly improves the detection performance and concentration range of the miniaturized NO detector.

The present invention discloses a catalyst and detection system for luminol electrochemiluminescence reaction and a preparation method thereof. The detection system adopts a Fe-N-C single-atom catalyst modified electrode. In an alkaline electrolyte, H2O2 is used as a co-reactant, and an electrochemiluminescence test can be performed at an ultra-low potential (≤±0.2V), which can achieve a strong electrochemiluminescence reaction of the luminol-H2O2 system. Wherein, the preparation method of the Fe-N-C single-atom catalyst includes: dissolving Zn(NO3)2·6H2O and Fe(NO3)3·9H2O in methanol to obtain a first mixed solution; adding a methanol solution of 2-methylimidazole to the first mixed solution to obtain a second mixed solution; allowing the second mixed solution to undergo a mixed reaction at 55-65°C, and calcining the solid obtained by the reaction at 850-950°C under an inert atmosphere to obtain the Fe-N-C SACs single-atom catalyst.

A method for determining the sequence of a target single-stranded polynucleotide is provided, comprising monitoring the sequential incorporation of nucleotides complementary to the target single-stranded polynucleotide wherein the nucleotides are each attached to a chemiluminescent marker that elicits a different luminescence kinetics or luminescence type, wherein each incorporated nucleotide is identified by detecting the luminescence kinetics or type of luminescence of the chemiluminescent reaction in which the chemiluminescent marker is involved and subsequent removal of the chemiluminescent marker.

The invention relates to the technical field of immunofluorescence detection reagents, in particular to a chemiluminescence reaction system of cerium dioxide-based alkaline phosphatase, a chemiluminescence immunodetection kit and application of the chemiluminescence reaction system and the chemiluminescence immunodetection kit. The chemical reaction system comprises coated magnetic beads and a growth differentiation factor 15 antibody containing a ceria-based alkaline phosphatase label, and the surface of each coated magnetic bead is coated with an anti-growth differentiation factor 15 antibody and streptavidin; wherein the weight m1 of the coated magnetic beads and the weight m2 of the antibody marked with the ceria-based alkaline phosphatase and used for marking the growth differentiation factor 15 meet the relational expression that m1: m2 is smaller than or equal to 400: 1. According to the chemiluminescence reaction system, ceria-based alkaline phosphatase is used for replacing traditional alkaline phosphatase, and the stability and accuracy of chemiluminescence immunodetection of the growth differentiation factor 15 can be synchronously improved.

The invention relates to the field of biological detection, and particularly discloses a method and a kit for determining platelet antibodies and cross-matching types by a chemiluminescence method. The detection method comprises the following steps: firstly, adding tool platelets / donor platelets into a reaction hole, adding a sample to be detected and magnetic bead particles coated with platelet antibodies into the reaction hole, and incubating to obtain an immune complex; mixing the immune complex with an acridinium ester labeled anti-human IgG antibody, and incubating to obtain a reaction complex; and mixing the reaction compound with a pre-excitation liquid and an excitation liquid of acridinium ester in sequence, carrying out a chemiluminescence reaction, and calculating the concentration of the platelet antibody in the sample to be detected. The kit not only can be used for effectively screening platelet antibodies in a to-be-detected plasma sample, but also can be used for carrying out cross-matching detection before platelet infusion, so that a more accurate basis is provided for evaluating the platelet infusion effect and formulating a treatment scheme.

A test method and a sample analysis device based on a chemiluminescence reaction, a first type of luminescence signal containing a lower layer of reaction liquid and a second type of luminescence signal containing an upper layer of reaction liquid are obtained, and whether the test is abnormal is judged according to the first type of luminescence signal and the second type of luminescence signal. The application provides a scheme for judging whether the test is abnormal.

The present invention provides a method, device and computer equipment for correcting a chemiluminescent reaction curve, and relates to the field of instrument detection technology. The method comprises: obtaining a photon counter characteristic curve, wherein the photon counter characteristic curve reflects the relationship between a standard light intensity value and a light intensity measurement value, wherein the light intensity measurement value refers to a light intensity value measured by a photon counter, and the standard light intensity value refers to a light intensity value obtained by controlling current conversion after calibration of a standard light source; obtaining a chemiluminescent reaction curve, wherein the chemiluminescent reaction curve reflects the relationship between the reaction time and the light intensity measurement value; and correcting the chemiluminescent reaction curve in sections according to the photon counter characteristic curve to obtain a corrected reaction curve. The present invention indirectly improves the linear measurement range of the photon counter by using the photon counter characteristic curve to correct the chemiluminescent reaction curve in sections without changing the hardware device.

The invention relates to the technical field of injection molds, and discloses a precise injection mold based on a chemiluminescence reaction cup, the precise injection mold comprises a fixed mold, a demolding mold, a movable mold and a material port opening and closing mechanism, and further comprises a mounting seat fixedly mounted on one side of the movable mold, and two linkage pneumatic control mechanisms fixedly mounted on the two sides of the movable mold; according to the technical scheme, the conical feeding port is controlled to be opened and closed through the material port opening and closing mechanism, the conical feeding port is closed, residual materials are discharged, and the integrity of the bottom of the reaction cup and the injection molding quality are guaranteed; the linkage pneumatic control mechanism controls the operation of the mold along with opening and closing of the mold, the change of gas pressure in the mold cavity is controlled, a negative pressure environment is realized or exhaust operation is performed, and the gas content in the mold cavity is reduced due to the negative pressure environment, so that the probability that bubbles are generated in the chemiluminescence reaction cup is reduced; the shaped reaction cup is pushed away from the second die cavity through the exhaust operation, so that the reaction cup is quickly separated from the inner wall of the die cavity.

The invention discloses a blending device for chemiluminescence detection and application of the blending device, and relates to the technical field of analysis and detection.The blending device comprises a hollow motor, a chemiluminescence reaction tank support, a mounting support, a chemiluminescence reaction tank, a power source, a motor driver and a speed regulation controller; a chemiluminescence reaction tank bracket is mounted on the hollow motor, and the axis of the chemiluminescence reaction tank bracket and the axis of the hollow motor are not collinear. By arranging the eccentric structure or the inclined structure of the bracket of the chemiluminescence reaction tank, the reaction solution can rotate along with the hollow motor and simultaneously introduces irregular circular motion, so that the turbulent flow in the solution is increased, the uniform mixing effect is improved, the solution can be quickly and uniformly mixed in a reaction system to reach a balanced state, stable chemiluminescence is generated, and the reaction efficiency is improved. The repeatability of an experiment and the relative standard deviation of a parallel experiment are obviously improved, and high-sensitivity detection of cadmium ions is realized.

The invention discloses preparation of ABEI macrolide and application of ABEI macrolide in preparation of an AD diagnostic kit, ABEI macrolide is adopted as a marker, the chemiluminescence reaction does not need to depend on horse radish peroxidase (HRP) and a traditional enhancer, and a detection system is simpler. The AD diagnostic kit provided by the invention comprises a reagent 1 containing magnetic beads labeled by a p-Tau181 antibody, a reagent 2 containing a p-Tau181 antibody labeled by ABEI macrolide, a p-Tau181 antigen standard substance and excitation liquid, and the provided AD diagnostic kit has the advantages of high stability, simplicity and convenience in operation, light drifting resistance, high sensitivity and low cost, and can provide more accurate detection guarantee for clinical diagnosis.

This invention belongs to the field of pathogen activity analysis technology. It discloses an optical chemiluminescence trace analysis system for pathogenic bacteria. The system includes: constructing a set of discrete reaction sites of the target pathogenic bacteria on an immobilized capture interface in the sample; performing a chemiluminescence reaction on the set of discrete reaction sites to generate a local chemiluminescence response sequence and a luminescence spatial distribution sequence; obtaining the spatial response trajectory of the target pathogenic bacteria by analyzing the lighting sequence of each discrete reaction site and the expansion and aggregation characteristics in the site's neighborhood; obtaining the pathogenic bacteria activity determination result by analyzing the consistency of continuous luminescence at different discrete reaction sites and the branching and converging characteristics of the outward expansion path in the spatial response trajectory; and outputting a conclusion on the presence of pathogenic bacteria. This invention improves the stability of trace pathogenic bacteria detection and enhances the ability to distinguish weak positives and activity.

A reagent strip and a sample analyzer. The reagent strip comprises: a capture rod, which is coated with a capture agent used for capturing a target analyte in a sample solution to be tested; a label chamber, in which a labeling reagent carrying a label is pre-encapsulated, wherein the labeling reagent can bind to the target analyte; a substrate chamber, in which a signal-generating reagent is pre-encapsulated, wherein the signal-generating reagent can undergo a chemiluminescent reaction with the label to obtain a solution to be tested; and a measurement chamber, in which no liquid is pre-placed, wherein the measurement chamber is used for accommodating at least part of the solution to be tested, such that the solution to be tested in the measurement chamber can be tested by means of an optical signal generated by the chemiluminescent reaction. The test strip and the sample analyzer can improve the testing accuracy.

The invention discloses an optical measurement system of a full-automatic POCT high-precision immunity analyzer, the optical measurement system comprises a reaction bin part, an optical measurement part and a substrate separate injection part, the reaction bin part comprises an incubation assembly, the incubation assembly comprises an incubation table and a side detection convex clamping seat, and a reaction cup comprises a reaction cup upper cup sleeve; the optical detection component is used for performing optical detection on contents in the reaction cup and comprises a light receiving detection assembly, and the light receiving detection assembly comprises a side detection light receiving seat; the substrate separate injection part is used for injecting substrate liquid into the reaction cup and comprises an injection head; when light emitting detection is carried out, the side detection light receiving seat and the side detection convex clamping seat are embedded to form a first multi-concave-convex-ring embedded structure, the filling head and the upper cup sleeve of the reaction cup are embedded to form a second multi-concave-convex-ring embedded structure, and the first multi-concave-convex-ring embedded structure and the second multi-concave-convex-ring embedded structure form a plurality of light closing matching surfaces. The chemiluminescence reaction in the reaction cup is carried out in a stable and reliable closed environment, the interference of an external light source is reduced, and the detection specificity and sensitivity are improved.

A method for enhancing the chemiluminescent signal of a chemiluminogenic compound in a chemiluminescent reaction mixture in a chemiluminescent reaction which has a step of oxidizing the chemiluminogenic compound in the presence of an effective amount of an enhancer can be performed. To obtain an enhanced chemiluminescent signal, a compound selected from the group of quaternary amine cationic substances of formula (I) for enhancing the chemiluminescent signal of an acridinium compound is used, preferably an acridinium ester or an acridinium sulfonamide. The signal is obtained in a chemiluminescent reaction, preferably in a chemiluminescent immunoassay, a related chemiluminescent composition and a related kit.

To provide a metal oxide nanozyme for solving the problem of insufficient enzyme activity caused by easy aggregation of small and medium sized nanozymes, and to provide a method for producing the same.SOLUTION: To provide a metal oxide nanozyme confined in an MOF, a method for producing the same, and a method for detecting ascorbic acid. The metal oxide nanozyme confined in the MOF is a complex formed by ZnIrOx clusters and ZnIrMOFs, and the ZnIrOx clusters are uniformly grown on the surface of the ZnIrMOFs. The metal-oxide nanozyme confined in the MOF can catalyze the chemiluminescent reaction of oxidizing luminol in the H2O2, thereby achieving highly sensitive and specific detection of ascorbic acid.SELECTED DRAWING: Figure 1

The present invention relates to a method for enhancing the chemiluminescent signal of a chemiluminescent compound in a chemiluminescent reaction mixture in a chemiluminescent reaction, the method comprising oxidizing the chemiluminescent compound in the presence of an effective amount of an enhancer to obtain an enhanced chemiluminescent signal, the use of a compound selected from the group consisting of quaternary ammonium cationic substances of formula (I) for enhancing the chemiluminescent signal of an acridinium compound, preferably an acridinium ester or an acridinium sulfonamide, in a chemiluminescent reaction, preferably in a chemiluminescent immunoassay, and related chemiluminescent compositions and kits.

The present invention provides a method for enhancing electrochemiluminescence reaction by in-situ induction of micro-nano bubbles on the surface of boron-doped diamond. The method of the present invention comprises the steps of: using a boron-doped diamond electrode as a working electrode, a platinum mesh as a counter electrode, and Ag / AgCl as a reference electrode to construct an electrochemical reaction system; using electrochemical cyclic voltammetry (CV) or chronoamperometry (i‑t) to drive the surface of the working electrode to generate micro-nano bubbles and construct a three-phase interface microenvironment; using electrochemical cyclic voltammetry (CV), linear sweep voltammetry (LSV) or chronoamperometry (i‑t) to drive the electrochemiluminescence reaction. The present invention uses an electrochemical method to induce micro-nano bubbles in situ on the surface of boron-doped diamond, construct a special three-phase (gas, liquid, solid) interface microenvironment, and then promote the interface electrochemiluminescence reaction. The method of the present invention has the advantages of in-situ enhancement, simple operation and controllable at any time.

The present invention relates to the technical field of biology. Disclosed are a chemiluminescence reaction detection reagent and detection method for the content of spermine in seminal plasma. The chemiluminescence reaction detection reagent comprises a luminol-containing sodium hydroxide solution, a copper ion solution and a sodium borate buffer solution. According to the present invention, on the basis of the chemiluminescence reaction principle of metal ions catalyzing hydrogen peroxide to oxidize luminol, it is found that the luminescence reaction intensity of copper ions and a luminol reagent would be remarkably influenced in the presence of spermine. On this basis, the present invention develops a kit for detecting the content of spermine on the basis of static chemiluminescence analysis, and establishes a new method for static chemiluminescence analysis of spermine, wherein the method involves high sensitivity, simple operation, small sample volume and low costs, and improves the working efficiency.

The present disclosure relates to a chemiluminescence analyzer. The chemiluminescence analyzer comprises: a sample preparation apparatus, which is configured to mix a blood sample to be tested and a reaction reagent in a reaction vessel, so as to obtain a sample solution to be tested; a substrate providing apparatus, which is configured to provide a luminescent substrate into the reaction vessel containing said sample solution, so as to carry out a chemiluminescence reaction; a photometric apparatus, which at least comprises a photometer and is configured to detect a luminescent signal generated in the chemiluminescence reaction; and a data processing apparatus, which is configured to obtain, on the basis of the luminescent signal and a preset calibration model, a test result of a substance to be tested in said blood sample. A first linear measurement range of the photometer is [A1, A2] photons per second, wherein A1 is less than or equal to 2000, and A2 is greater than or equal to 108. A second linear measurement range of the luminescent substrate is [B1, B2] photons per second, wherein B1 is less than or equal to 3000, and B2 is greater than or equal to 108. By means of the embodiments of the present disclosure, a chemiluminescence analyzer having a high sensitivity and a high upper limit of linear measurement can be obtained.

The invention discloses a human epididymal protein 4 determination kit based on homogeneous chemical immunoassay, the human epididymal protein 4 determination kit comprises a homogeneous chemiluminescence reaction system and a reaction buffer solution, the homogeneous chemiluminescence reaction system comprises: a, labeling: coupling a specific antibody with a chemiluminescence marker to form a labeled antibody, a luminous donor molecule is used for marking a human epididymal protein 4 specific antibody 1 to form a first reagent, and a luminous acceptor molecule is used for marking a human epididymal protein 4 specific antibody 2 to form a second reagent; b, adding a sample: under the action of exciting light with a certain wavelength, combining the two antibodies with the human epididymal protein 4 in a sample to be detected to form a sandwich immune complex; according to the human epididymal protein 4 determination kit based on the homogeneous chemical immunoassay, efficient and accurate determination of the human epididymal protein 4 is realized by optimizing a homogeneous chemiluminescence reaction system and a reaction buffer solution.

The application discloses a pair of chiral covalent organic framework materials (CCOFs) based on a dynamic coordination strategy, which are prepared by using Co(II) as a coordination metal center, taking a covalent organic framework Co-TFB-DABP constructed by Co(II) as a main skeleton, and adopting chiral molecules with reversible coordination groups such as carboxyl, sulfydryl and amino for reversible coordination. The chiral covalent organic framework material obtained by the application can catalyze a luminol-hydrogen peroxide chemiluminescence reaction, and when a chiral drug exists, the chiral covalent organic framework material can also have a stereospecific interaction with the chiral drug to produce a differential chemiluminescence response, thereby realizing enantioselective recognition, and therefore, the chiral covalent organic framework material can be used for simply, reliably and highly sensitively detecting chiral drugs such as ibuprofen enantiomers.

The invention relates to a double-antibody-based nucleic acid assembly amplification chemiluminescence detection method applied to the field of biological detection. The method comprises the following steps: S1, coupling a nucleic acid chain with a detection antibody; s2, coupling a signal molecule with a nucleic acid chain; s3, incubating the coupling products in S1 and S2 in a nucleic acid hybridization solution to obtain a luminous detection mother solution, and S4, directly replacing acridinium ester labeled antibody components or antibody coupled magnetic bead components with the obtained luminous detection mother solution for detection in a chemiluminescence immunoassay, and carrying out two different amplification detection operations from a magnetic bead end or a detection end. Dozens to hundreds of single antibody labels are realized, and the influence of interfering substances in a sample on detection is reduced while the chemiluminescence reaction sensitivity is improved.

A method for increasing the light emission produced by the chemiluminescent reaction of 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, a peroxidase or a conjugate thereof, an enhancer, a co-enhancer and a peroxide oxidant, wherein the enhancer is an anionic N-alkylphenothiazine and the co-enhancer is selected from 4-dialkylaminopyridine or N-azole, and wherein the method comprises the steps of: i. achieving a chemiluminescent substrate by mixing together 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H)-dione, the enhancer, the co-enhancer and the peroxide oxidant, and ii. adding the peroxidase or a conjugate thereof to the chemiluminescent substrate.

A method for determining the sequence of a target single-stranded polynucleotide is provided, comprising monitoring the sequential incorporation of nucleotides complementary to the target single-stranded polynucleotide, wherein the nucleotides are each attached to a chemiluminescent marker that induces a different luminescence kinetics or luminescence type, wherein each incorporated nucleotide is identified by detecting the luminescence kinetics or luminescence type of the chemiluminescent reaction in which the chemiluminescent marker participates and subsequently removing the chemiluminescent marker.