33 results about "Weakly positive" patented technology

The invention relates to the technical field of biology, particularly the field of viral antibody detection. A kit for detecting the antibody against Peste des petits ruminants virus b-ELISA comprises the following ingredients which are arranged respectively: Peste des petits ruminants nucleoprotein antigen, Peste des petits ruminants monoclonal antibody, diluent, strong positive serum, weak positive serum, negative serum, HRP sheep anti-mouse secondary antibody, 20 times the concentration of washing liquid, substrate liquid, stopping solution and enzyme-linked immunosorbent plate. The optimum proportion of each ingredient in the kit is determined by experiments. The kit can be used for rapid diagnosis and detection of animal Peste des petits ruminants virus antibody, especially for the antibody detection of a lot of samples in the epidemiological survey of Peste des petits ruminants. The detection method of Peste des petits ruminants virus b-ELISA has different detection principle and experiment operating procedures and the like from those of a c-ELISA detection method in a BIRAD laboratory. The Peste des petits ruminants nucleoprotein antigen and Peste des petits ruminants monoclonal antibody in the kit are self-developed. The detection sensitivity, singularity and other indexes of the kit are the same with those of the c-ELISA detection method in the internationally recognized BIRAD laboratory.

The present invention provides a human immunodeficiency virus type 1 one-step fluorescence quantitative RT-PCR detection kit, which comprises a RT-PCR reaction solution, a RT-PCR enzyme mixture, a primer probe, a negative control, a strong positive control, a weak positive control, and calibration substances No.1-5. According to the present invention, a one-step fluorescence quantitative reaction can be directly performed on the extracted HIV-1RNA, the virus loading of the HIV-1RNA in a sample can be detected, the reference gene is adopted as the internal control, and the UNG enzyme is adopted to prevent pollution; and the kit has characteristics of simple one-step amplification method, short procedure, easy operation, pollution prevention, strong detection result specificity, high sensitivity, clear result and high result reliability, and can be used for detection of the virus loading of the human immunodeficiency virus type 1 (HIV-1) RNA in blood serum.

The invention discloses a kit stored at 2 to 8 DEG C and used for rapidly detecting hepatis c virus nucleic acid. The kit comprises a qRT-PCR reaction solution, a qRT-PCR initiator, an RNA internal control, a strongly positive quality control product, a weakly positive quality control product, a negative quality control product and a quantitative reference product; and the qRT-PCR reaction solution comprises a qRT-PCR reinforcing agent, a reaction buffer solution, two specific primers for detecting the hepatis c virus nucleic acid, one specific probe for detecting the hepatis c virus nucleic acid, two specific primers for detecting the RNA internal control, one specific probe for detecting the RNA internal control, a bi-directional DNA polymerase, UNG enzyme, dATP, dGTP, dCTP, dUTP and 0.1percent sodium azide. The kit is high in sensitivity (the minimum detection limit of the HCV virus is 10IU/ml), good in specificity, high in precision, accurate in qualification, and wide in linear range, simple in detection method, short in detection time, high in reference value for clinically and rapidly detecting the hepatis c virus nucleic acid, and suitable for popularization and application.

The invention discloses an incomplete antibody detection kit which comprises a U-shaped micro-pore plate, an aqueous glue chromatography medium and granular antigen, wherein the U-shaped micro-pore plate is wrapped with antihuman globulin; the aqueous glue chromatography medium is used for incubating a mixture of serum or plasma with the granular antigen; the granular antigen is reacted with an incomplete antibody in the serum or plasma to form an antigen-antibody compound. The invention further relates to an incomplete antibody detection method. A granular antigen-antibody compound is separated from free antibodies in a centrifugal or magnetic mode, and a tedious washing process in a conventional method can be replaced. When results are observed, if the incomplete antibody is sensitized on the granular antigen, the incomplete antibody can be captured by the antihuman globulin and is spread at the bottom of the U-shaped micro-pore plate, and a positive result is judged; if sensitization is not resulted, the incomplete antibody is not captured but being focused at the bottom to form a small point, and a negative result is judged; a weakly positive result is judged if a situation falls in between. By adopting the method, operation steps are greatly simplified, the detection efficiency can be improved, results are easy to judge, in addition, a great amount of samples can be detected simultaneously, and automation can be achieved easily.

The invention provides a traditional Chinese medicine health-care cervical vertebra pillow. The cervical vertebra pillow comprises a pillow sleeve 1 and a pillow inner 2 located in the pillow sleeve 1. The pillow inner 2 comprises, by weight, 20 parts to 25 parts of agastache, 21 parts to 26 parts of mints, 10 parts to 12 parts of schisandra chinensis, 85 parts to 110 pars of semen cassiae and 90 parts to 120 parts of tartary buckwheat. The clinic use proves that according to the clinical symptoms and signs of cervical spondylosis and the corresponding physical examination result, after the traditional Chinese medicine health-care cervical vertebra pillow is used, the corresponding clinical symptoms are obviously relieved, the specialized examination result which is originally positive is converted to be weakly positive or negative, and the average service life is 19.64 days.

The invention discloses a kit for detecting human cytomegalovirus. The kit comprises specific PCR primers and a fluorescent probe for detecting human cytomegalovirus DNAs, specific primers and a probefor detecting a DNA internal standard, a PCR reaction buffer, a mixed enzyme liquid, theDNA internal standard, a negative control, a strongly positive control, a weakly positive control and quantitative references A, B, C and D; wherein the sequences of the primers and the probes are as shown in SEQ ID No. 1-6. The kit of the invention has the advantages of convenient and quick detection, capacity of quantitatively detecting human cytomegalovirus within 20 min, high sensitivity, the lowest detection limit of 34.5 IU/ml, and good specificity. According to the invention, a pair of primers are designed according to the internal repetitive sequence [mu]l65, so the primers have no cross-interference with other pathogens and have high specificity. The kit of the invention can be traced to the World Health Organization (WHO) standard unit IU/mL; quantitative results have traceability; and the kit has high reference value for clinical diagnosis of diseases related to human cytomegalovirus andis suitable for promotion and application.

The invention relates to a brucellosis complement fixation-enzyme-linked immunosorbent assay (CF-ELISA) diagnostic kit, and the kit combines a reaction system in a complement fixation test (CFT) and an enzyme marker amplification system in an enzyme-linked immunosorbent assay (ELISA). The brucellosis complement fixation-enzyme-linked immunosorbent assay (CF-ELISA) diagnostic kit has the characteristics of high sensitivity, easy and fast use, high throughput and high degree of standardization compared with a traditional complement fixation test diagnostic reagent, has the characteristics of high specificity and capability of detecting a variety of brucellosis specific antibodies compared with a traditional ELISA diagnostic kit, and is an ideal new brucellosis diagnostic tool. The kit comprises the following main components: a lipopolysaccharide LPS antigen-coated plate, strongly-positive control serum, weakly positive control serum, negative control serum, a guinea pig complement, enzyme labeled guinea pig complement C1q-B monoclonal antibody 60G4, a substrate coloring-developing solution, a stop solution and washing liquid.

The aim of the invention is to provide a carcinoembryonic antigen (CEA) rapid simple detection kit and making and application methods thereof, the carcino-embryonic antigen rapid simple detection kit is lower in cost than a test strip; by use of specific responses of human carcinoembryonic antigen and anti carcinoembryonic antigen antibody, the carcinoembryonic antigen and the antibody are bonded onto a NC (nitrocellulose) membrane and colloidal gold particles, a colloidal gold and antibody complex is added onto the NC membrane, and whether the CEA is negative or positive is determined by observation of the color. When the CEA content is lower than 2.5uM, a 2.5uM CEA standard product is not significantly different from a 0uM standard product in color development, so when the CEA content is lower than 2.5uM, the CEA is determined to be negative; when the CEA content is in the 2.5-10uM range, the detection point color is significantly shallower than the 0uM spot color, the CEA is determined to be weak positive; when the CEA content is more than 10uM, and no red spot appears, the CEA is determined to be positive.

The invention discloses an influenza A and B virus nucleic acid detection kit. The influenza A and B virus nucleic acid detection kit comprises a nucleic acid amplification reaction solution, an enzyme mixed solution, an influenza A/B virus mixed reaction solution, a positive control solution, a weakly positive control solution and a negative control solution, wherein the influenza A/B virus mixedreaction solution comprises an influenza A virus upstream primer, an influenza A virus downstream primer, an influenza A virus probe, an influenza B virus upstream primer, an influenza B virus downstream primer, an influenza B virus probe, an internal reference RNase P upstream primer, an internal reference RNase P downstream primer, an internal reference RNase P probe and DEPC water, and the internal reference means the detection of the internal reference of the product. The internal reference of the embodiment participates in the process from nucleic acid extraction to RT-PCR, and can be used for monitoring the collection, storage and transportation of samples and the nucleic acid extraction process and amplification process, so that false negative interpretation results are avoided.

A particle agglutination-evaluating container for immunological analysis, based on an agglutinate formed in an agglutination reaction between an antibody- or antigen-containing sample and agglutination particles, includes a transparent container body having a bottom face including a sloping bottom face and a raised bottom face extended in a horizontal direction at the top of the sloping bottom face to form an obtuse angle with the sloping bottom face, and a fluidal separation layer containing insoluble particles which are filled in the container body and where the agglutination particles are separated according to the agglutination degree, wherein agglutination is evaluated by observation from the bottom of the container body, where the agglutination reaction is judged positive when the agglutination particles are observed through the raised bottom face, negative when observed at the bottom end of the sloping bottom face, and weakly positive when observed in the middle of the sloping bottom face.

The invention provides an immunochromatography detection reagent strip, a kit containing the immunochromatography detection reagent strip and application of the immunochromatography detection reagent strip, and relates to the technical field of biological detection. According to the immunochromatography detection reagent strip, an immunochromatography technology is adopted, COVID-19 N protein, influenza A virus NP protein and influenza B virus NP protein serve as detection antigens, and fluorescent microspheres, latex, colloidal gold or colloidal carbon are coupled with specific protein to obtain a specific protein compound; the nasopharyngeal swab, oropharyngeal swab, or saliva of the patient is then detected. The reagent strip has the advantages of high sensitivity, high specificity and quantitative detection, and avoids leak detection of weak positive samples and false detection of false positive samples to the greatest extent. Meanwhile, the immunochromatography used in the invention is rapid in detection, results can be obtained within 15 minutes, and field operation can be realized through simple training.