The invention discloses a kit stored at 2 to 8 DEG C and used for rapidly detecting hepatis c
virus nucleic acid. The kit comprises a qRT-PCR reaction solution, a qRT-PCR initiator, an
RNA internal control, a strongly positive
quality control product, a
weakly positive quality control product, a negative
quality control product and a quantitative
reference product; and the qRT-PCR reaction solution comprises a qRT-PCR reinforcing agent, a reaction
buffer solution, two
specific primers for detecting the hepatis c
virus nucleic acid, one specific probe for detecting the hepatis c
virus nucleic acid, two
specific primers for detecting the
RNA internal control, one specific probe for detecting the
RNA internal control, a bi-directional
DNA polymerase, UNG
enzyme, dATP, dGTP, dCTP, dUTP and 0.1percent
sodium azide. The kit is high in sensitivity (the minimum
detection limit of the HCV virus is 10IU / ml), good in specificity, high in precision, accurate in qualification, and wide in
linear range, simple in detection method, short in detection time, high in reference value for clinically and rapidly detecting the hepatis c virus nucleic acid, and suitable for popularization and application.