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Immunochromatography detection reagent strip, kit containing immunochromatography detection reagent strip and application of immunochromatography detection reagent strip

An immunochromatographic detection and reagent strip technology, which is applied in the field of kits containing immunochromatographic detection reagent strips, can solve problems such as the failure of vaccines and antibodies, and achieves the advantages of avoiding missed detection of weak positive samples, high sensitivity and rapid detection. Effect

Active Publication Date: 2021-12-10
HANGZHOU BAOLIN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the existing technology, there are only about 10 glycosylation sites of influenza virus, and as long as there are 3-4 site mutations, the influenza virus will mutate, and our vaccination against influenza every year will be ineffective; the glycosylation site of the new coronavirus is 65- 70, which is 7 times that of influenza. If the glycosylation site of the new coronavirus mutates, new recombinant strains or hybrid recombinant strains will appear, and vaccines and antibodies will be completely ineffective.

Method used

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  • Immunochromatography detection reagent strip, kit containing immunochromatography detection reagent strip and application of immunochromatography detection reagent strip
  • Immunochromatography detection reagent strip, kit containing immunochromatography detection reagent strip and application of immunochromatography detection reagent strip
  • Immunochromatography detection reagent strip, kit containing immunochromatography detection reagent strip and application of immunochromatography detection reagent strip

Examples

Experimental program
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Effect test

Embodiment 1

[0071] Embodiment 1 prepares immunochromatography detection reagent strip:

[0072] Such as figure 1 Shown, a kind of immunochromatography detection reagent strip, the preparation method of described reagent strip comprises the following steps:

[0073] The chromatographic reaction membrane containing the new coronavirus N protein antibody, influenza A virus NP protein antibody, and influenza B virus NP protein antibody on the detection line; contains fluorescent microspheres, latex, colloidal gold or colloidal carbon and specific protein The label binding pad of the specific protein complex obtained by coupling, as well as the sample pad and the absorbent paper are lapped and adhered to the PVC backboard;

[0074] Among them, the sample pad and the quantum dot marker binding pad are lapped on one end of the chromatographic reaction membrane in turn, and the absorbent paper is lapped on the other end of the chromatographic reaction membrane, and then the parameters of the cut...

Embodiment 2

[0076] Example 2 Novel coronavirus / influenza A virus / influenza B virus antigen one card three test kit Sensitivity test for novel coronavirus antigen:

[0077] Take 3×10 respectively 5 TCID 50 / ml of the original strain of novel coronavirus and the inactivated virus sample of Delta, first diluted 10 times with PBS buffer, then carried out 2 times serial dilution, and diluted 11 times in total. All dilution gradient samples were checked with the fluorescent PCR detection kit of Daan Gene Company, and the operation was carried out according to the instructions to determine the corresponding Ct value of each sample.

[0078] Take 300 μL samples of each dilution gradient and 300 μL sample detection kit buffer and mix 5 times. After standing for 2 minutes, take 90 μL of the above-mentioned buffer-treated samples and add them to the reagent injection wells. Each diluted sample and each reagent are repeated for 1 person. Take the reading after 15 minutes of dropping the sample, use...

Embodiment 3

[0088] Example 3 Novel Coronavirus / Influenza A / Influenza B Antigen One Card Three Test Reagent Sensitivity Test of Influenza A Virus and Influenza B Virus Antigen:

[0089] Experimental steps:

[0090] Take two inactivated virus samples of influenza A virus and influenza B virus respectively (Flu A / GUANGDONG / 2019, Flu A / NYMC X-223A, Flu B / Washington / 02 / 2019, Flu B / phuket / 3073 / 2013), the initial concentration of FluA was 7.5×10 5 TCID 50 / ml, the initial concentration of Flu B was 1.0×10 6 TCID 50 / ml.

[0091] Dilute 10 times each with normal saline to make 500ul, and then dilute 8 concentrations with test buffer 5 times, that is, dilute the original solution by 10, 50, 2.5×10 2 , 1.25×10 3 , 6.25×10 3 , 3.125×10 4 , 1.5625×10 5 and 7.8125×10 5 Times, a total of 4 × 8 = 32 standard solutions of different concentrations to be tested; take out the new coronavirus / influenza A virus / influenza B virus antigen combined detection reagent, and load the above 4 strains of in...

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Abstract

The invention provides an immunochromatography detection reagent strip, a kit containing the immunochromatography detection reagent strip and application of the immunochromatography detection reagent strip, and relates to the technical field of biological detection. According to the immunochromatography detection reagent strip, an immunochromatography technology is adopted, COVID-19 N protein, influenza A virus NP protein and influenza B virus NP protein serve as detection antigens, and fluorescent microspheres, latex, colloidal gold or colloidal carbon are coupled with specific protein to obtain a specific protein compound; the nasopharyngeal swab, oropharyngeal swab, or saliva of the patient is then detected. The reagent strip has the advantages of high sensitivity, high specificity and quantitative detection, and avoids leak detection of weak positive samples and false detection of false positive samples to the greatest extent. Meanwhile, the immunochromatography used in the invention is rapid in detection, results can be obtained within 15 minutes, and field operation can be realized through simple training.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a kit containing the immunochromatographic detection reagent strip and its application. Background technique [0002] There is no clinically effective small-molecule chemical drug for the treatment of new coronavirus pneumonia. The accumulation of new coronavirus mutations has evolved into immune evasion, and therapeutic neutralizing antibodies are also ineffective. According to the existing technology, there are only about 10 glycosylation sites of influenza virus, and as long as there are 3-4 site mutations, the influenza virus will mutate, and our vaccination against influenza every year will be ineffective; the glycosylation site of the new coronavirus is 65- 70, which is 7 times that of influenza. If the glycosylation site of the new coronavirus mutates, new recombinant strains or hybrid recombinant strains will appear, and vaccines and antibodies will be comple...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/569G01N33/58
CPCG01N33/558G01N33/56983G01N33/582G01N33/585G01N33/587G01N2333/165G01N2333/11G01N2469/10Y02A50/30
Inventor 陈科达陈喆王燕青陈秋强
Owner HANGZHOU BAOLIN BIOTECHNOLOGY CO LTD
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