Method for expanding hematopoietic stem cells

A technology of hematopoietic stem cells and cells, which is applied in the field of macrophage colony-stimulating factor to proliferate hematopoietic stem cells, and can solve problems such as no one has reported successful experiments

Inactive Publication Date: 2005-04-13
DAIICHI SEIYAKU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as of now, no one has reported a successful experiment with this

Method used

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  • Method for expanding hematopoietic stem cells
  • Method for expanding hematopoietic stem cells
  • Method for expanding hematopoietic stem cells

Examples

Experimental program
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Effect test

Embodiment approach

[0032] The present invention is specifically described with reference to the following examples, but the present invention is not limited to the described

[0033] Example.

Embodiment 1

[0035] (Collection of bone marrow cells)

[0036] Bone marrow cells were collected from the femur and tibia of C57BL / 6J-Ly5.1 mice (Jackson Laboratory), suspended in Hunks Balanced Salt Solution (Gibco-BRL) containing 5% fetal calf serum (FBS, Immunbiological Laboratory) (hereinafter referred to as HBSS), a suspension of planktonic cells was prepared using a 1 ml syringe fitted with a 22-gauge needle. The suspended cell suspension was filtered using a 40 μm pore size nylon mesh (Falcon) to remove cell aggregates. Washing was performed by centrifugation at 1,100 rpm for 5 minutes, followed by resuspension in HBSS to form a single-cell suspension of bone marrow cells.

[0037] (Preparation of Lin-negative or weakly positive cells)

[0038] Using StemSep TM (StemCell Technologies INC.) prepared mouse hematopoietic precursor cells, and prepared differentiation antigen-negative or weakly positive (Linfigure 1 As shown, the collected cells were Lin

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Abstract

A method for culturing Lin-negative or weakly positive cells in the presence of macrophage colony-stimulating factor (M-CSF); a method for growing Lin-negative or weakly positive cells by culturing them in the presence of M-CSF A method for hematopoietic stem cells; a population of hematopoietic stem cells obtained by the proliferation method; a kit containing M-CSF for cultivating Lin-negative or weakly positive cells; and a kit containing M-CSF for proliferating hematopoietic Reagents for stem cells.

Description

technical field [0001] The invention relates to a method for proliferating hematopoietic stem cells with macrophage colony-stimulating factor (M-CSF). More precisely, the present invention relates to a method for culturing Lin-negative or weakly positive cells in the presence of M-CSF, a method for proliferating Lin-negative or weakly positive cells by culturing them in the presence of M-CSF A method for hematopoietic stem cells, a hematopoietic stem cell population obtained by the proliferation method, a kit containing M-CSF for culturing Lin-negative or weakly positive cells, and a kit containing M-CSF for proliferating Reagents for hematopoietic stem cells. Background technique [0002] Hematopoietic stem cells are defined as cells that have both the ability to generate all blood cells such as red blood cells, white blood cells, platelets, T and B lymphocytes (pluripotency) and the ability to renew themselves (self-renewal capacity). In bone marrow transplantation, hema...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/078C07K14/535C12N5/02C12N5/071C12N5/0789
CPCC12N5/0647C12N2501/22C12N5/00C12N5/0634
Inventor 名和克彦莲村麻衣今田千晴
Owner DAIICHI SEIYAKU CO LTD
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