Influenza A and B virus nucleic acid detection kit and use method

Abstract

The invention discloses an influenza A and B virus nucleic acid detection kit. The influenza A and B virus nucleic acid detection kit comprises a nucleic acid amplification reaction solution, an enzyme mixed solution, an influenza A / B virus mixed reaction solution, a positive control solution, a weakly positive control solution and a negative control solution, wherein the influenza A / B virus mixedreaction solution comprises an influenza A virus upstream primer, an influenza A virus downstream primer, an influenza A virus probe, an influenza B virus upstream primer, an influenza B virus downstream primer, an influenza B virus probe, an internal reference RNase P upstream primer, an internal reference RNase P downstream primer, an internal reference RNase P probe and DEPC water, and the internal reference means the detection of the internal reference of the product. The internal reference of the embodiment participates in the process from nucleic acid extraction to RT-PCR, and can be used for monitoring the collection, storage and transportation of samples and the nucleic acid extraction process and amplification process, so that false negative interpretation results are avoided.

Description

technical field [0001] The invention belongs to the technical field of influenza virus nucleic acid detection, and specifically relates to a type A and type B influenza virus nucleic acid detection kit and a use method. Background technique [0002] Influenza is an acute respiratory infectious disease caused by influenza virus. It occurs frequently in spring and winter, spreads rapidly, has a high incidence rate, and has a short epidemic period. It is easy to cause outbreaks or pandemics, and it is difficult to control. Influenza viruses include three types: A, B, and C. Type A is the most likely to cause epidemics, followed by Type B, and Type C rarely causes epidemics. Influenza virus is mainly transmitted through air droplets, often causing fever, fatigue, muscle aches and mild to moderate respiratory symptoms, severe cases can cause pneumonia, myocarditis and heart failure. Because the infection symptoms and epidemic characteristics caused by these viruses are similar, ...

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Problems solved by technology

[0006] The purpose of the present invention is to solve the problem that the existing influenza A virus and influenza B virus detection are not convenient enough and difficult to evaluate the accuracy of the results

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