Six respiratory virus nucleic acid detection kit and use method

Abstract

According to a six respiratory virus nucleic acid detection kit and a use method, whether respiratory syncytial virus, respiratory adenovirus, human metapneumovirus, parainfluenza virus type I, parainfluenza virus type II and parainfluenza virus type III exist or not is detected in an A tube and a B tube of the same reaction system, the A tube is used for detecting parainfluenza virus type I, respiratory adenovirus and human metapneumovirus, and comprises probes which are respectively marked with FAM, VIC and CY5, the B tube is used for detecting respiratory syncytial virus, parainfluenza virus type II and parainfluenza virus type III, and comprises probes which are respectively marked with FAM, VIC and CY5, the A / B tubes are provided with an internal reference control, the internal reference control is a human conservative gene RNase P fragment, an internal reference probe is marked with ROX, and the internal reference control can display the false negative, so that the occurrence offalse negative interpretation results caused by inhibitors existing in a sample or operation errors is avoided, and thereby the accuracy of PCR detection is improved.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, and specifically relates to a six-item respiratory virus nucleic acid detection kit and a use method. Background technique [0002] At present, respiratory tract infection is a common clinical disease, which can be caused by various viruses. Common respiratory viruses include: respiratory syncytial virus, respiratory adenovirus, human metapneumovirus, parainfluenza virus, influenza A virus, influenza B virus, etc. Respiratory virus infection can cause a variety of diseases, such as cold, flu, pharyngitis, laryngitis, bronchitis, pneumonia, etc. The main clinical symptoms after human infection include fever, runny nose, sore throat, cough, headache and diarrhea. Because the symptoms of infection caused by these viruses are similar to those of influenza, it is difficult to distinguish the pathogen by clinical symptoms alone. Laboratory diagnostic methods include virus isolation, ant...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the existing respiratory virus nucleic acid detection is difficult to detect multiple viruses at the same time and it is difficult to effectively control the detection process

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