18 results about "RNase P" patented technology

The invention provides a respiratory system fungal infection detection system, a kit and a preparation method thereof, and belongs to the technical field of biological detection. The primer probe composition used by the respiratory system fungal infection detection system comprises upstream and downstream primers and a fluorescent probe sequence designed aiming at paracoccus braziliensis, upstream and downstream primers and a fluorescent probe sequence designed aiming at histoplasma capsulatus, and upstream and downstream primers and a fluorescent probe sequence designed aiming at troptosporidium. The kit comprises upstream and downstream primers and fluorescent probe sequences designed for budgettes dermatitis, upstream and downstream primers and fluorescent probe sequences designed for Sirpolyspora sessilifolia, upstream and downstream primers and fluorescent probe sequences designed for talaromyces marneffei, upstream and downstream primers and fluorescent probe sequences designed for internal reference designed for ribonuclease P, upstream and downstream primers and fluorescent probe sequences designed for ribonuclease P, upstream and downstream primers and fluorescent probe sequences designed for ribonuclease P, and upstream and downstream primers and fluorescent probe sequences designed for ribonuclease P, the kit can be used for effectively detecting the fungi of the respiratory system, and ensures high accuracy, specificity and sensitivity.

The invention discloses a positive quality control product for detecting breast cancer and ovarian cancer gene large fragment deletion and a preparation method thereof, the gene comprises breast cancer and ovarian cancer related genetic genes BRCA1 / BRCA2 / ATM / PALB2, the positive quality control product is a recombinant plasmid containing four gene segments including a first exon of a to-be-detected gene, a plant sequence, a last exon of the gene and RNase P; the method comprises the following steps: inserting a human genome RNase P sequence into a plasmid vector according to a fragment ratio of 2: 1, and verifying the copy number of a to-be-detected gene by adopting a fluorescent quantitative qPCR (quantitative polymerase chain reaction) method so as to judge whether the obtained plasmid can be used as a positive quality control product for detection or not, thereby completing the quality inspection of the positive quality control product; the preparation method provided by the invention is simple and easy to operate and good in stability, the positive quality control product obtained by adopting the preparation method can be used as a positive internal standard quality control material for detecting breast cancer and ovarian cancer gene large fragment deletion, the whole experiment process is monitored, and the accuracy of experiment and detection results is improved.

The invention provides a monkey pox virus nucleic acid detection kit and a preparation method thereof. The kit is used for qualitatively detecting monkey pox virus nucleic acid in vitro. Specific primers and probes are designed for the target F3L gene and the internal standard RNase P gene respectively, and the primer and probe combination is excellent in specificity, stability, compatibility, anti-interference performance and instrument adaptability, high in accuracy and sensitivity, easy to operate and high in detection speed. According to the kit disclosed by the invention, a freeze-drying reagent is prepared by utilizing the primer probe, so that the kit can completely get rid of the limitation of a cold chain, convenience is provided for remote areas lacking cold chain transportation and long-distance transportation, and the transportation and storage cost is greatly reduced. The monkey pox virus nucleic acid detection kit is simple in preparation method, good in reproducibility and easy to realize large-scale production and clinical application.

The invention relates to the technical field of gene detection, in particular to a reagent and a kit for detecting human papilloma virus genotyping by using a four-channel fluorescently-labeled melting curve method and application of the reagent and the kit. A 4X Lyo-PCR buffer freeze-drying amplification reagent is matched with a designed specific primer and probe combination; the kit can be directly prepared into freeze-dried powder by using a unique all-component freeze-drying technology, and can be directly redissolved during use, so that the sensitivity of a freeze-dried reagent is improved to 0.2 copy / uL, and meanwhile, the kit can amplify and classify 28 human papilloma viruses in a PCR reaction, so that the 28 human papilloma viruses can be subjected to typing detection at the same time, and the kit is suitable for large-scale popularization and application. The sensitivity of each type reaches 5 copies / reaction, meanwhile, an internal reference system of endogenous ribonuclease P is introduced and used for monitoring sample sampling and sample extraction quality so as to avoid false negative of a detection result, and higher detection flux, sensitivity and specificity are achieved.

The application belongs to the technical field of molecular diagnosis, and particularly relates to a digital PCR kit and method for accurately detecting the copy number of a CAR gene of a CAR-T cell and a lentivirus vector. The application first realizes the absolute quantification of CAR, Rev and an internal reference gene RNase P in a single tube simultaneously, and has strong universality. Based on the principle of digital PCR, the absolute copy number is directly obtained without a standard curve, and the result is accurate and highly repeatable. The detection lower limit reaches 0.01%, and extremely low abundance CAR-T residues or microresidual diseases can be detected. Primers and probes are designed for the conservative regions of CD3zeta, Rev and RNase P, and no cross reaction is verified, so that false positives are effectively avoided. The sample only needs gDNA, and there is no requirement for cell activity, and the sample is compatible with freezing and transportation. The kit is premixed and optimized, the process is automated, and 4-5 hours are needed. The kit is suitable for the whole chain of CAR-T drug research and development, production process quality control, clinical patient monitoring and safety evaluation.

The invention provides a primer-probe composition, a kit and a preparation method thereof for detecting the pathogen of chromoblastomycosis, and belongs to the technical field of biological detection. The primer-probe composition comprises an upstream primer, a downstream primer and a fluorescent probe designed for Helminthosporium; an upstream primer, a downstream primer and a fluorescent probe designed for Cladosporium; an upstream primer, a downstream primer and a fluorescent probe designed for Ochrasporium; an upstream primer, a downstream primer and a fluorescent probe designed for Cladosporium; an upstream primer, a downstream primer and a fluorescent probe designed for Vanadium; and an upstream primer, a downstream primer and a fluorescent probe designed as an internal reference for a human RNase P gene. The invention adopts a multiplex fluorescent PCR technology combined with a freeze-drying technology, is easy to operate, overcomes the false positive phenomenon, and has high sensitivity and strong specificity, and can simultaneously detect the infection conditions of five pathogens causing chromoblastomycosis.

The invention discloses a positive quality control product for detecting colorectal cancer gene large fragment deletion and a preparation method thereof, the gene contains hereditary colorectal cancer related genes MLH1 / MSH2 / MSH6 / PMS 2 / EPCAM / MUTYH / STK11, and the positive quality control product is a recombinant plasmid containing four gene fragments of a first exon of a gene to be detected, a plant sequence, a last exon of the gene and RNase P. A human genome RNase P sequence is inserted into a plasmid vector according to a fragment ratio of 2: 1, and the copy number of a to-be-detected gene is verified by adopting a fluorescent quantitative qPCR method, so that whether the obtained plasmid can be used as a positive quality control product for detection is judged, and the quality inspection of the positive quality control product is completed. The preparation method provided by the invention is simple and easy to operate and good in stability, and the positive quality control product obtained by adopting the preparation method can be used as a positive internal standard quality control material for detecting large fragment deletion of the hereditary colorectal cancer gene, so that the whole experiment process is monitored, and the accuracy of experiment and detection results is improved.

The application belongs to the technical field of molecular biology detection, and particularly relates to a composition for detecting mycobacterium tuberculosis complex and application thereof. The application provides a primer pair and a probe for detecting mycobacterium tuberculosis complex, and simultaneously uses IS6110 and IS1081 genes specific to the mycobacterium tuberculosis complex as detection targets, and introduces a RNase P endogenous internal standard gene fragment, and the primer and the probe are respectively designed according to the conservative sequences of the IS6110 and IS1081 genes of the mycobacterium tuberculosis complex, and the primer and the probe have good accuracy, specificity and sensitivity for detecting the mycobacterium tuberculosis complex. The results of the examples show that the detection accuracy of the primer pair and the probe provided by the application for the mycobacterium tuberculosis complex can reach 100%, and no fluorescence signal is found for detecting other non-mycobacterium tuberculosis species.

The invention discloses a freeze-dried microsphere for detecting novel coronavirus nucleic acid and a preparation method thereof, and the freeze-dried microsphere comprises a first upstream primer, a first downstream primer and a first probe which are designed in a novel coronavirus specific target N gene region, the first upstream primer, the second downstream primer and the second probe are designed in a novel coronavirus specific target ORF1ab gene region, the third upstream primer, the third downstream primer and the third probe are designed in a novel coronavirus specific target S gene region, and the fourth upstream primer, the fourth downstream primer and the fourth probe of a specific human housekeeping gene RNase P are designed in a novel coronavirus specific target S gene region. The kit comprises the following components: a reaction buffer solution, a UNG enzyme, a reverse transcriptase, a DNA polymerase, a freeze-drying protective agent and DEPC water. The freeze-dried microspheres can be stored at room temperature for a long time, the reagent performance is good, tedious operation in the liquid preparation process of an existing liquid type novel coronavirus nucleic acid detection reagent is simplified, experimental errors are reduced, and pollution is avoided.

The invention discloses a monkey pox virus triple fluorescence PCR nucleic acid detection kit, a detection method and application. The kit adopts a single-tube triple detection system and comprises (a) a monkey pox virus universal detection primer probe group and a targeted F3L and G2R gene conserved region; (b) a Clade Ib subtype detection primer probe group which targets D14L and A36R gene deletion feature regions; (c) a Clade II subtype detection primer probe group, which is used for targeting J2R and D18L gene feature sequences; and (d) an internal standard detection group which targets the human RNase P gene. By optimizing the concentrations of the primers and probes and four-channel fluorescent labels (FAM universal type, VI-Clade Ib, ROX-Clade II and CY5 internal label), single-tube synchronous detection is realized. The leak detection risk is reduced by adopting a double-target redundancy design, virus screening and subtype identification (Clade Ib / II) can be completed through single detection, and an internal standard system monitors the quality of a sample in the whole process. The kit is suitable for clinical early diagnosis, epidemic prevention and control and strain traceability.

The present invention demonstrates the utility of small-molecule microarray (SMM) screening method used to identify novel small-molecule scaffolds that bind to long non-coding RNAs (lncRNAs), thereby expanding the possibilities for RNA-targeted therapeutics. RNase P is a ribozyme that is essential for 5'-maturation of precursor tRNAs. Disclosed herein is an inhibitor of the Methanobrevibacter smithii RNase P RNA (Msm RPR). The inhibitor comprises of a diaryl-piperidine compound, termed M1, to inhibit Msm RPR. Given the similarity of Msm RPR to RPRs from similar archaea in the rumen, the present invention provides support for the idea of mitigating methane production by altering rumen tRNA metabolism.

The present invention demonstrates the utility of small-molecule microarray (SMM) screening method used to identify novel small-molecule scaffolds that bind to long non-coding RNAs (lncRNAs), thereby expanding the possibilities for RNA-targeted therapeutics. RNase P is a ribozyme that is essential for 5'-maturation of precursor tRNAs. Disclosed herein is an inhibitor of the Methanobrevibacter smithii RNase P RNA (Msm RPR). The inhibitor comprises of a diaryl-piperidine compound, termed M1, to inhibit Msm RPR. Given the similarity of Msm RPR to RPRs from similar archaea in the rumen, the present invention provides support for the idea of mitigating methane production by altering rumen tRNA metabolism.

The invention provides a primer probe composition and a kit for detecting chromoblastosis pathogens and a preparation method of the primer probe composition and the kit, and belongs to the technical field of biological detection, the primer probe composition comprises an upstream primer, a downstream primer and a fluorescent probe which are designed aiming at helminthosporium; the upstream primer, the downstream primer and the fluorescent probe are designed for mycospora; the upstream primer, the downstream primer and the fluorescent probe are designed for ochracea; the upstream primer, the downstream primer and the fluorescent probe are designed aiming at the cladosporium; the upstream primer, the downstream primer and the fluorescent probe are designed for Wansella; designing an upstream primer, a downstream primer and a fluorescent probe of an internal reference aiming at the human RNase P gene; according to the invention, a multiple fluorescent PCR technology is combined with a freeze-drying technology, so that the operation is simple and convenient, the false positive phenomenon is overcome, the sensitivity is high, the specificity is strong, and the infection conditions of the five pathogens causing the chromoblastosis can be detected at the same time.

A composition for detecting the presence of monkey pox virus in a biological sample comprises a monkey pox nucleic acid forward primer; a monkey pox nucleic acid reverse primer; and monkey pox nucleic acid probes. The method includes a multiplex assay including a set of three channels, each channel having a single assay, including a monkey pox assay with a reporter factor and a quencher, a non-ceiling positive pox assay with a reporter factor and a quencher, and an RNase P assay with a reporter factor and a quencher. A kit for detecting monkey pox virus nucleic acid includes assay components including a forward primer and a reverse primer and a probe for each of the three assays in the multiplex assay.

The present invention relates to a method for improving the yield of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the yield of a difficult-to-express recombinant protein by using a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, the expression of large recombinant proteins, difficult-to-express proteins and useful proteins can be significantly increased by reducing the expression of the rnpA gene.

The present invention relates to a primer probe group and a kit for detecting monkeypox virus, and belongs to the technical field of molecular biology. The present invention provides a primer probe group for detecting monkeypox virus, and the primer probe group includes a primer probe group targeting F3L gene, a primer probe group targeting B6R gene, and a primer probe group targeting RNase P gene. The primer probe group effectively avoids mutual interference between multiple primer probe groups through sequence design, and the primer probe group uses RNase P gene as an internal standard to monitor the collection, transportation and extraction process of the sample to be tested, effectively avoiding false negatives in the test results. Therefore, the use of the primer probe group to perform monkeypox virus quantitative detection on the sample to be tested has the advantages of high accuracy, strong specificity and high sensitivity, and has great application prospects.

This invention discloses a polypeptide targeting mitochondrial ribonuclease P and its application in the preparation of antitumor drugs. Based on the principle of the inhibition of mtRNA P by the endogenous natural peptide AC027045.3, this invention designs a novel polypeptide based on the amino acid sequence of the binding functional domain of AC027045.3. This polypeptide is designed based on the natural polypeptide sequence and functions by mimicking the endogenous physiological regulatory pathway. Compared to the natural peptide, this novel polypeptide has a shorter sequence, which is beneficial for chemical synthesis. Given the high energy dependence of actively proliferating tumor cells, this invention uses mtRNA P as a novel target for tumor therapy, designing a targeting peptide to inhibit the mitochondrial oxidative phosphorylation process and exert an anti-cancer effect.

A detection panel for the universal detection of SARS-like coronaviruses and SARS-CoV-2 viruses and a method for using the detection panel are provided. The detection panel may be known as a Center for Discovery and Innovation (“CDI”) detection panel (CDI Enhanced COVID-19 Test) and may also include an additional primer / probe set in the detection panel to detect the human RNase P gene (RP) in control samples and clinical specimens. The detection panel is designed for both universal detection of SARS-like coronaviruses using an E gene (envelope) detection assay, and specific detection of the SARS-CoV-2, using an N2 (nucleocapsid) detection assay.