Fully-premixed freeze-drying multi-fluorescent PCR detection kit for novel coronavirus, influenza A virus and influenza B virus and detection method thereof
A type of influenza B virus and detection kit technology, which is applied in the field of in vitro diagnosis and biological detection, can solve the problems of time consumption and impact on virus detection rate, increase stability, avoid repeated freezing and thawing and multiple packaging, The effect of a low level of operation
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] 1. The lyophilized solid-state RT-PCR Mix contains primer sets and probes corresponding to the N gene of SARS-CoV-2, the M gene of influenza A, the M gene of influenza B, and the RNAse P primer sequence of the internal reference gene of human origin. The solid-state RT-PCR Mix mainly contains the N gene of the new coronavirus (SARS-CoV-2), the M gene of the influenza A virus, the M gene of the influenza B virus and the specificity of the genome of the internal standard gene RNaseP before lyophilization. Primers and probes, dNTP, Taq DNA polymerase, reverse transcriptase, RNase inhibitor, UDG enzyme and freeze-dried compound protective agent, and the balance is DEPC treated water. The specific components and contents are shown in Table 2.
[0040] Table 2: Novel Coronavirus and Influenza A, Influenza B Virus RT-PCRMix Liquid
[0041]
[0042]
[0043] Preparation steps of freeze-dried solid-state novel coronavirus and influenza A and influenza B virus RT-PCR mix pa...
Embodiment 2
[0072] The operation method of embodiment 2 detection kit
[0073] The operation method of the freeze-dried rapid fluorescent quantitative PCR detection kit for human new coronavirus, type A and type B influenza virus:
[0074] Including the following steps:
[0075] (1) Template RNA extraction, using a nucleic acid extraction kit to extract RNA from the sample to be tested.
[0076] (2) Prepare reagents: add 15 μL of reconstituted Buffer RB to each PCR tube with freeze-dried particles, and add 50 μl of reconstituted Buffer RB to the freeze-dried vials of positive control and negative control;
[0077] (3) Adding samples and controls: According to the number of experimental samples, add 5ul of the extracted sample RNA to each of the above PCR tubes, and take 5ul of the negative and positive controls after reconstitution, and add them to the corresponding PCR tubes. Put the cap on the PCR tube.
[0078] (4) Centrifuge and put it into a fluorescent quantitative PCR instrument...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com