The invention discloses a joint detection kit for novel influenza virus and influenza virus A / B as well as preparation method and application of the joint detection kit. The joint detection kit comprises a detection test strip, the detection test strip comprises a sample pad, a combination pad, a detection pad and an absorption pad which are sequentially connected in an overlapped mode, and the detection pad is provided with a detection line and a quality control line which are parallel to each other; the detection line comprises a first detection line, a second detection line and a third detection line which are separated from one another, the first detection line is coated with a new coronavirus antibody, the second detection line is coated with an influenza A virus antibody, and the third detection line is coated with an influenza B virus antibody. The joint detection kit provided by the invention can simultaneously detect three items of the new coronavirus, the influenza A and the influenza B after one-time sampling treatment, is short in detection time and simple to operate, and avoids missed diagnosis or misdiagnosis in single item detection; besides, the repeated contact between medical staff and patients in multiple sample collection processes is reduced, and the risk of epidemic situation spreading is reduced.