Composition, kit and method for detecting and typing influenza virus and application of composition, kit and method

Abstract

The invention belongs to the field of molecular biological detection. In particular, the invention relates to the detection and typing of influenza viruses. More specifically, the invention relates to the detection and typing of influenza A virus. The invention provides a composition for detecting and typing influenza viruses, application of the composition, a kit comprising the composition and a method for using the composition. By using the composition disclosed by the invention, the influenza A / B does not need to be amplified by a plurality of pairs of primers, the influenza A / B can be simultaneously detected by only one pair of primers, the cost is low, the amplification is more uniform, the whole system is more accurate and stable, and meanwhile, the compatibility of the composition disclosed by the invention with other virus detection systems is better.

Description

technical field [0001] The invention belongs to the field of molecular biology detection; in particular, it relates to detection and typing of influenza virus. More specifically, it relates to the detection and typing of influenza A and B viruses. Background technique [0002] Acute upper respiratory tract infection is a general term for acute inflammation of the nasal cavity, pharynx or larynx. It is the most common infectious disease of the respiratory tract. 80% of the common causes are viruses, and a few are caused by bacteria. Patients were regardless of age, gender, occupation and region. Not only is it highly contagious, but it can also cause serious complications, so it should be actively prevented. Influenza bears the brunt of the disease, with 5% to 10% of adults and 20% to 30% of children suffering from influenza every year. Influenza virus (influenzavirus), referred to as influenza virus, is an RNA virus that causes influenza in humans and animals. Human inf...

AI Technical Summary

Problems solved by technology

[0004] At present, in the laboratory diagnosis methods, more PCR detection methods are still used, but the principle is to design primers and probes for the detection of influenza A virus and influenza B respectively for the nucleocapsid protein NP gene or the matrix protein M gene. The design of primer probes for viruses is complex, and the preparation of reagents is cumbersome, which is not conducive to large-scale production. Moreover, each additional pair of primers in the PCR amplification system increases the probability of forming primer dimers, and the interference between different primer probes increases. will increase to varying degrees, and the interaction between all primers will increase, which will inevitably affect the effect of amplification, thereby affecting the stability and accuracy of the entire detection system. The increase in primers will lead to an increase in cost. At the same time, There are many primers and probes in the amplification system, and it may also happen that one amplified fragment inhibits another amplified fragment, which leads to inhomogeneous amplification results, which also affects the stability and accuracy of the entire detection system.

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