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Pharmaceutical application of astragaloside compound

A technology of astragaloside IV and medicine, applied in the field of medicine, can solve the problems such as difficulty in preventing and controlling influenza

Active Publication Date: 2021-02-12
THE FIFTH PEOPLES HOSPITAL OF SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Flaws in vaccines and chemical drugs make it difficult to prevent and treat influenza

Method used

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  • Pharmaceutical application of astragaloside compound
  • Pharmaceutical application of astragaloside compound
  • Pharmaceutical application of astragaloside compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Astragaloside IV (AST) is to cytotoxicity assay experiment

[0031] When astragaloside IV AST was applied to MDCK cells at concentrations of 5 μg / ml, 10 μg / ml, 20 μg / ml, 40 μg / ml, 60 μg / ml, 80 μg / ml, and 100 μg / ml for 72 hours, the MDCK cells were treated at a concentration of astragaloside IV AST of 80 μg. When the concentration is 100μg / ml, there is some rounding under the light microscope, but there is no obvious toxicity, and when the concentration is 100μg / ml, there is obvious cell rounding. When the concentration is higher than 100μg / ml, the cytotoxicity is more obvious, and there is obvious cell aggregation phenomenon. The OD value of each hole was measured by MTT method, and the results showed that: when the concentration of MDCK cells was 100 μg / ml, the survival rate was more than 93%, while at 200 μg / ml, the survival rate was reduced to 30%, and when it exceeded 200 μg / ml, the cells were almost inactive ( Such as figure 1 shown). This shows tha...

Embodiment 2

[0032] Embodiment 2 Astragaloside IV (abbreviated as AST) to the inhibition experiment of influenza virus

[0033] 1. Drugs and reagents

[0034] Astragaloside IV (Astragaloside IV), measured by HPLC with a purity of ≥98%, was purchased from AMA Copoeia Reference (40mg), and was dissolved in DMSO when used; fetal bovine serum (FBS), tetramethylthiazolium blue (MTT), bovine serum Albumin (BSA) and dimethyl sulfoxide (DMSO) were purchased from Sigma, USA; trypsin-EDTA, penicillin, and streptomycin solutions were purchased from Gibco; secondary antibodies 488-labeled goat anti-mouse IgG (H+L) was purchased from Cell Signaling Technology; DAPI nuclei luminescent dye was purchased from Beyond Biotech Co., Ltd.

[0035] 2. Viruses and Cells

[0036]The throat swab specimens collected from influenza-like cases were subjected to nucleic acid and virus isolation, detection and typing, and one-step RT-PCR method was used to amplify the HA gene and NA gene of a selected influenza B vi...

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Abstract

The invention provides an application of an astragaloside compound or a pharmaceutical composition containing the astragaloside compound in preparation of an anti-influenza B virus drug. AstragalosideAST not only has an obvious inhibiting effect on proliferation of influenza B virus in MDCK cells, but also has no cytotoxic effect in a safe concentration range, is high in safety, is expected to become a clinical anti-influenza B medicine, and has a very wide application prospect.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to the medicinal use of astragaloside IV compounds. Background technique [0002] Influenza is an acute respiratory disease caused by influenza virus, which has the characteristics of wide range of hosts, rapid spread and high morbidity rate. Influenza virus is easy to cause influenza pandemic, and its mortality rate is higher than that of seasonal influenza, which poses a serious threat to human and animal life and health. Influenza A and B are two different types of viruses that cause influenza. In addition to infecting humans, influenza A virus also widely exists in animals such as poultry, pigs, horses, seals, whales, and minks. Influenza B viruses mainly circulate in the human body and cause seasonal influenza, which tends to be prevalent in winter and spring. Influenza A virus is a new subtype produced by antigen mutation, which often causes a worldwide pandemic. The vari...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P31/16A61P11/00
CPCA61K31/7048A61P31/16A61P11/00
Inventor 梅周芳揭志军陈鸿军
Owner THE FIFTH PEOPLES HOSPITAL OF SHANGHAI
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