82 results about "Virus isolation" patented technology

The invention discloses a GeXP (Gene Expression) rapid detection kit capable of simultaneously identifying six virus of chicken respiratory disease. The GeXP rapid detection kit is used basing on a CeXP system and contains seven PCR (Polymerase Chain Reaction) primer pairs, the specificity for simultaneously detecting avian influence virus, H5, H7 and H9 sub type of the avian influence virus, Newcastle disease virus, infectious bronchitis virus and infectious laryngotracheitis virus is strong, the sensitivity can be up to 100 copy / mu l, and compared with an identifying result of regular test methods such as virus isolation and hemagglutination inhibition, the coincidence rate is up to 100%. According to the GeXP rapid detection kit disclosed by the invention, a simple and high-throughput detection kit and a detection system are provided for the detection of common main chicken viral respiratory disease, the actual needs are accordant, and the application prospect is wide.

The invention discloses a multiplex RT-PCR detection kit for a porcine reproductive and respiratory syndrome virus. The kit comprises two pairs of primers used for amplification of genes of an American porcine reproductive and respiratory syndrome virus and a European porcine reproductive and respiratory syndrome virus; and the invention provides an application method for the detection kit. The invention has the following beneficial effects: the multiplex RT-PCR detection kit for the porcine reproductive and respiratory syndrome virus provided by the invention is fast compared with classical virus isolation, single RT-PCR and serum typing methods and can conveniently identify and distinguish any one belonging to American and European porcine reproductive and respiratory syndrome viruses in one shot so as to facilitate timely and fast responses to epidemic disease situations, regions and environments and implementation of correct treatment in shortest time; meanwhile, the Trizol extraction method for extraction of the whole genome RNA in the invention has the characteristics of rapidness, simpleness, low cost and usage of a small amount of desired reagents, is especially applicable to extraction of small samples and has wide market application prospects.

The invention discloses a method for detecting infectious bovine rhinotracheitis virus. Internal amplification control (IAC) markers indicating the false negative are added to a polymerase chain reaction (PCR) system; an IAC fragment is built through the rearrangement of the base, the design of the primers and the PCR gene amplification with the bypass method; the added concentration of an internal maker template is 10<2> MuL in each reaction process, the added concentration of the Mg2+ is 2.0 mmol / L, the added concentration of the each dNTP is 0.3 mmol / L, the added concentration of each primer is 0.5 mmol / L, and the added concentration of a probe is 0.2 mmol / L; the primers are amplified at 50 DEG C for 2min in 1 cycle, 95 DEG C for 5min in 1 cycle, 95 DEG C for 15s in 45 cycles and 60 DEG C for 60s in 45 cycles. Therefore, the existence of inhibitory components or the occurrence of false negative indication can be accurately showed. The method is 10-100 times more sensitive than the conventional PCR method and the virus isolation test method and can be widely used for detecting the infectious bovine rhinotracheitis virus.

The invention discloses a GeXP rapid detection kit for identifying eight types of porcine viral diseases and a primer group thereof. Nine pairs of specific primers and a pair of universal primers are designed by the inventor based on research of a GeXP system, accordingly, a GeXP rapid detection method for identifying the eight types of porcine viral diseases is established, and the corresponding detection kit is prepared. The GeXP rapid detection method and the detection kit have the advantages of good specificity, high sensitivity, convenience, high efficiency and the like and can be used for simultaneously detecting and identifying eight types of pathogens. Compared with identification results of conventional experiment methods such as virus isolation and hemagglutination inhibition test, the coincidence rate of the GeXP rapid detection method reaches 100%. The GeXP rapid detection method and the detection kit can be used for effectively solving amplification preference in a multiplex-PCR (polymerase chain reaction) process, provide simple, convenient and high-throughput detection means for detection of main porcine common infectious diseases and pathogens thereof, meet practical requirements and have a broad application prospect.

The invention discloses a medical efficient bacterium blocking filter material which is prepared by weaving gathered fibers. The invention also discloses a preparation method of the medical efficient bacterium blocking filter material, which comprises the following steps of: melting polyene macromolecular particles with 800-1500 high melt index to obtain a melt, and continuously delivering the melt to a linearly arranged porous fiber jet die orifice under the action of a DC high voltage electric field through a metering pump; leading the jet fibers in a molten state to a receiving device under the action of plasmas through a plasma launch device after leaving the die orifice; and collecting the fibers by a negative pressure forming device, and sticking the gathered fibers to obtain the medical efficient bacterium blocking filter material. The material has the effect of isolating bacteria; meanwhile, because the material is an electric material with polarity, the material has strong absorption performance on bacteria. The material can be used for manufacturing isolation and filter materials in various virus protection isolation cloths, protective masks, protective devices and virus isolation ventilation systems.

The invention belongs to the technical field of network virus defense, and discloses a novel computer network virus defensive system. The network virus defensive system comprises an end detection module, a virus monitoring module, an analysis module, a main control module, a virus feature library module, a virus feature matching module, a virus isolation module, a virus searching and killing module, and a repeated-virus-infection-prevention module. The end detection module, the virus monitoring module and the analysis module are connected with the main control module through a circuit line separately; the main control module is connected with the virus feature library module, the virus feature matching module and the virus isolation module separately through the circuit line separately. Through the repeated-virus-infection-prevention module, the regenerating capability of viruses can be eradicated; through the analysis module, botnet viruses or target attack viruses which are targetedaiming at virus attacking objects can be analyzed, searched and killed, and therefore compared with general antivirus software, the novel computer network virus defensive system is more targeted.

The invention relates to the field of health and medical treatment, in particular to a nano-virus isolation spraying agent for a mask. The nano-virus isolation spraying agent is a new material for spraying on the surface of a common mask to be converted into a medical protective mask and is a spraying product and technology for transforming the surface of the common mask into a medical bacterial isolation mask after spraying and is an upgraded product of the medical protective mask. The nano-virus isolation spraying agent is prepared from the following raw materials in percentage by weight: electrostatic nano-fiber particles, tourmaline, an adhesive and the balance of water or alcohol. The nano-virus isolation spraying agent can be used for conventional common masks made of different materials, the application range is wide, the common masks have the virus protection function and the PM2.5 blocking capacity, haze gas is effectively prevented from invading, and repeated use of the masksis not affected.

The invention relates to the technical field of USB flash disk virus isolation, in particular to a USB flash disk virus isolator and a working method thereof.The USB flash disk virus isolator comprises a main control chip used for strictly controlling and checking data access; the storage module is used for caching the isolated virus files; the network interface module is accessed to a local areanetwork by adopting an international general network protocol; the USB interface module is used for being externally connected with a USB flash disk; the display module is used for visually displayingthe equipment operation state; the storage module, the network interface module, the USB interface module and the display module are respectively connected with the main control chip through corresponding lines; uSB flash disk data is filtered and screened through a built-in system program of the main control chip; the system program automatically detects and identifies the safety file of the white list and the virus file of the black list, the safety content on the white list is allowed to be connected with the PC through the local area network to be read and written, and once the system program scans the content on the black list, the corresponding content is immediately isolated and hidden, so that virus spreading caused by misoperation of a client is avoided.

The invention belongs to the technical field of protection covers and in particular discloses a professional medical operator sanitation protection mask. Aiming at the problems that a protection maskis poor in protection property, cannot play a very good role of virus isolation, is not applicable to long-term use and is low in wearing comfort, a wearer can feel relatively hard to breath, languagecommunication of medical operators with the mask can be affected, and the like, the invention discloses the professional medical operator sanitation protection mask which comprises a battery box anda plastic shell, wherein a control box is arranged on the outer wall of the top end of the plastic shell; a rubber facial mask is adhered to the outer wall edge of one side of the plastic shell; and ascrew hole is formed in the middle of the outer wall of one side of the plastic shell. By adopting the mask, the mask can be kept internally dry, the wearing comfort is improved, bacterium breeding environments are avoided, odor is avoided, relatively fresh air can be supplied to a user, the odor in the air can be absorbed, the smell of the air can be improved, bacteria in the air can be killed,and the bacterium killing effect can be improved.

The invention discloses a soft computer and a realization method thereof, which pertains to the computer technology field. The soft computer comprises an intelligent terminal, an access environment and a backup computer. The intelligent terminal comprises a memory body being a memory medium of data and software and an externally connected body, wherein, the memory body, in respect of memory space, is divided into a data zone, a virus isolation zone and a software zone, wherein, application software, an operation system and a drive program are stored in the software zone, and the externally connected body at least comprises a CPU, a hardware anti-virus module and a user authentication module. A high-speed interface is provided by the CPU for connection with the data of the memory body and the CPU at least comprises a data input interface and a data output interface. The access environment comprises external equipment for input and output, display equipment and a computation platform with data computation and handling capacity. The backup computer is a computer accessed through a network. The soft computer has advantages of good portability, strong individuation, automatic data backup and excellent anti-virus performance and safety.

The invention discloses a primer pair for identifying newcastle disease virus and multi-subtype avian influenza virus and an application thereof. The PCR primer pair composition provided by the invention comprises primer pair NDV, primer pair H3V, primer pair H5V, and primer pair H9V; the PCR primer pair composition is applicable to the preparation of reagents for the diagnosis or auxiliary diagnosis of newcastle disease and avian influenza, or to the preparation of reagents for the identification or auxiliary identification of newcastle disease virus and multi-subtype avian influenza virus. When the PCR primer pair composition provided by the invention is used to simultaneously detect newcastle disease virus, H3, H5 and H9-subtype avian influenza virus, the specificity is strong; the sensitivity is 1EID50 / 100 microliters, 1EID50 / 100 microliters, 1*10-2EID50 / 100 microliters, and 1EID50 / 100 microliters respectively; compared with identification results of routine test methods such as virus isolation and hemagglutination inhibition tests, the results obtained by using the PCR primer pair composition of the invention has a coincidence rate of up to 100%; the PCR primer pair composition provided by the invention is applicable to the development of corresponding multiplex RT-PCR kits for the clinical diagnosis and epidemiological control of newcastle disease and avian influenza.

The invention discloses a CCN integrated modularized negative pressure isolation ward. The CCN integrated modularized negative pressure isolation ward comprises a first changing isolation room, a second changing isolation room, a ward area, a treatment area, a warehouse and a second isolation buffer room, wherein the ward area comprises a patient passage and a plurality of wards, a first isolationbuffer room is formed between the first changing isolation room and the patient passage, the treatment area comprises a treatment room system and a treatment area passage, and the second changing isolation room communicates with the treatment area passage; the treatment room system comprises a nurse station room, a treatment room and a doctor office room, and the treatment area passage communicates with the warehouse and the second isolation buffer room separately; and the first isolation buffer room is a shower room or an air shower room, and the second isolation buffer room is a shower roomor an air shower room. According to the CCN integrated modularized negative pressure isolation ward, the negative pressure isolation ward is established in a modularized manner, and viruses can be effectively prevented from spreading in the isolation ward and spreading to the outside, so that a remarkable isolation effect is acted, and virus isolation treatment, and epidemic prevention and control are benefited.

The invention relates to a virus vaccine A-NDV-LX / 14 for a newcastle disease, which is a recombinant of expressed genes VII F and HN, and the preservation number is CGMCCNO: 3844. After two weeks of the constructed virus A-NDV-LX / 14 immunity test on chicken, the average potency of the serum HI is 27. After four days of eliminating toxic material with immune group LaSota, the virus isolation ratesof SPF chicken laryngotracheal and cloacal swab samples are 10 percent and 30 percent respectively, and the virus isolation rates of chicken laryngotracheal and cloacal swab samples are 40 percent and 20 percent respectively. After four days of eliminating toxic material with immune group V4, the virus isolation rates of SPF chicken laryngotracheal and cloacal swab samples are 30 percent and 10 percent respectively, and the virus isolation rates of chicken laryngotracheal and cloacal swab samples are 30 percent and 20 percent respectively. Compared with traditional vaccine, more effective immune protection can be provided by the recombinant virus vaccine.

The invention provides a special prime designed according to conservative M genes of influenza virus. By the aid of the principle of specific binding of biotin and streptavidin, biotin-labeled PCR (polymerase chain reaction) products are bound on a microtiter plate, anti-digoxin peroxidase is added, and finally TMB (tetramethylbenzidine) color development solution is added, so that positive products can develop colors. On the basis, a special kit is successfully prepared. The method combines PCR with a high-sensitivity digoxin detection system, the sensitivity of the method is 100 times higher than that of a conventional agarose gel electrophoresis detection method, and the method is high in specificity and overcomes difficulty in detecting AIV (avian influenza virus) of a low content in a tissue sample. Compared with a virus isolation method, the method has the advantages that coincidence rate can reach more than 95%, pollution of chemical agents (such as ethidium bromide) is eliminated, automation is benefited, the method is suitable for detecting a large number of samples, and a new approach is provided for early diagnosis of avian influenza and molecular epidemiological investigation.

The invention provides a computer network security system, a use method, equipment and a storage medium. The computer network security system comprises a central processing unit, a network virus detection unit, a virus searching and killing unit, a virus isolation unit, a data encryption unit and a data transmission unit. The network virus detection unit is used for detecting network viruses in acomputer network in real time. The virus isolation unit is used for isolating the detected network viruses and storing the network viruses in an isolation region. The virus searching and killing unitis used for searching and killing network viruses in the isolation region. The data encryption unit is used for performing encryption processing on the network data packet so as to prevent network virus invasion. The data transmission unit is used for performing encryption transmission on the encrypted network data packet again. The method is simple in working principle and high in safety, detection, isolation, searching and killing of network viruses can be achieved, network data packets can be encrypted, data can be encrypted and transmitted, and data leakage can be effectively prevented.

A reovirus vaccine and its method for preparation belong to the technical sphere of druggist sundries and are used to solve the indigenous fowl reovirus infection vaccine problem. The separated reovirus is of CGMCC No.1713 as its preserved number and has been preserved in China microbial bacterial preservation management committee common microorganism center on May 16th, 2006. The said vaccine is prepared by steps including virus isolation from organ of diseased chicken, serum check, virus breeding and generation and cryodesiccation. The invention separates the virus from the diseased chicken with ARV infection of indigenous fowls, applies them in immune chicken, and produces immune response within 1 week and produces strong immunity within 2 weeks. The immune time is 2-2.5 months, and the detoxicating protecting rate during immune period is 94.8%-98.8%, which is explicitly higher than import vaccine protecting rate and can make up for the domestic defect of vaccine vacancy in reovirus affection.

The invention discloses a Seneca virus isolation strain, Seneca virus disease inactivated vaccine and a preparation method of the Seneca virus disease inactivated vaccine. A swine Seneca virus CH-HLJstrain is separated from a pig farm with symptoms of suspected Seneca virus infections in the Heilongjiang Province, the complete genome sequence of the strain is shown in SEQ ID No.1 in the description, and the microbial preservation number is CGMCC NO.18851. The invention further provides a method for preparing the Seneca virus disease inactivated vaccine. The method comprises the following steps: (1) performing viral multiplication and inactivation; (2) heating a vaccine adjuvant so as to obtain an oil phase; (3) heating a virus liquid so as to obtain a water phase; and (5) mixing and emulsifying the oil phase with the water phase into a double-phase oil emulsion. Swine immunological experiment results show that the Seneca virus disease inactivated vaccine prepared by the invention is capable of stimulating a body to generate an antibody of a high level. Immunoprotection efficacity evaluation results of the inactivated vaccine show that the inactivated vaccine prepared by the invention is capable of providing good protection on attacking of swine Seneca viruses.

The invention relates to a primer group and a high-sensitivity detection kit for carrying out spot quick detection on shrimp covert mortality nodavirus. The primer group comprises six primers. The high-sensitivity detection kit comprises a sampling tube, a rinsing tube internally filled with distilled water, a nucleic acid denaturation tube internally filled with a TE buffer solution, an amplification detection tube internally filled with an amplification reaction liquid and a dye, a negative control tube, a positive control tube, an FTA membrane and a quick drying fluid thereof. The primer group can be used for specifically distinguishing and efficiently amplifying target nucleic acid of detected CMNV (covert mortality nodavirus). The detecting method adopting the high-sensitivity detection kit has specificity, sensitivity and convenience which are higher than those of an electron microscope detecting method, a histopathology detecting method, a virus isolation detecting method and the like, is low in cost, convenient to use, relatively accurate and quick in detection, and extremely high in sensitivity.

The invention discloses a multiple PCR detection primer group and kit for rapidly differentiating the PCV1 type from the PCV3 type. The primer group includes primers L1, L2 and L3, wherein L1 represents 5'-ACCAGCGCACTTCGGCAGCGGCAGCA-3', L2 represents 5'-GCATTGTTCACCAGTACCCA-3', and L3 represents 5'-AAGTCCCCTTCCTGCGTCCGCTAATCT-3'. The special kit has very high sensitivity, and minimum detection quantities for the PCV1 type and the PCV3 type are 1*10<-7> ng / mL and 2*10<-7> ng / mL respectively; the specificity is high, and the amplification results of porcine reproductive and respiratory syndromevirus, classical swine fever virus, porcine pseudorabies virus, porcine parvovirus, porcine epidemic diarrhea virus, transmissible gastroenteritis virus and porcine rotavirus are negative; the repeatability is good, the same sample is detected for 3 times at an interval of one month, and the same result is obtained; moreover, the coincidence rate can reach 95% or above compared with methods of virus isolation, IFA and the like, and the primer group and the kit can be widely used in clinical typing detection of the PCV1 type and the PCV3 type.

The invention discloses a micropterus salmoides brain cell line and application thereof, the micropterus salmoides brain cell line has been preserved in the China Center for Type Culture Collection on March 25, 2021, and the preservation number is CCTCC NO: C202166. The micropterus salmoides brain cell line MSBr is stable in passage, has been successfully passed for more than 65 generations at present, is good in growth state, is wide in application range, not only can be used for virus isolation and identification, pathogen function research, environmental pollutant detection, gene screening and function analysis, fish tumor research and the like, but also can be used for preparing the micropterus salmoides brain cell line MSBr. Meanwhile, the method can also be used for monitoring and diagnosing viral diseases of aquatic animals, investigating and researching epidemiology, developing diagnostic kits for related viral diseases, developing vaccines and the like, and has relatively high application value and social and economic value.

The invention discloses a cherry valley duck embryo epithelial cell line and an establishment method thereof, and belongs to the field of cytobiology. The method comprises the steps of taking duck embryo tissue with 9 to 12 age in days in a sterile way, shearing the duck embryo tissue into tissue blocks, adhering the tissue blocks downwards to the bottom of a culture hole, adding nutrient solution into a carbon dioxide incubator, and performing plate adhering culture at 37 DEG C. The high-survivability and high-purity cherry valley duck embryo epithelial cell line is obtained according to the method and conducted to biological preservation, compared with primary cell, the cell line has the advantages that the cellular morphology and the physiological property of the cell line are obvious different from that of the primary cell, continuous and stable passage can be realized, the nutritional requirement is low, the growth cycle is short, and the cell line is suitable for large-scale culture, provides a large amount of high-quality cell material for life sciences, such as gene engineering, cell engineering, immunology, molecular biology and the like, can serve as donor cell for poultry somatic cell clone breeding, and can also serve as a main material for duck virus isolation and vaccine production.

The invention discloses infectious disease ward special isolation equipment convenient to use. The equipment comprises a ward body and also comprises a first fan, a ventilating duct, a second fan, a disinfection mechanism and a buffer zone, wherein the first fan body is in screw connection with the front end of the right side of the upper surface of the ward body; the ventilating duct is arrangedon the upper surface of the ward body and is in sealing connection with the first fan through the right side of the front end of the ventilating duct; the second fan is positioned on the rear end of the left side of the ward body and is in sealing connection with the left end of the ventilating duct; the disinfection mechanism is in sealing connection with the second fan; and the buffer zone is arranged on the front side of the inner cavity of the ward body along a left and right direction. According to the infectious disease ward special isolation equipment convenient to use, negative pressure is generated in the ward to prevent bacteria from escaping to the outside along with air, bacteria which fly out along with air when a worker enters and leaves can be killed so as to isolate the bacteria in the ward body, the air which is discharged from the ward is disinfected, isolation safety is guaranteed, a filter core can be conveniently replaced, and the equipment is convenient to maintain.

An industrial network security system comprises a central processing unit, an identity verification module, a display module, a vulnerability detection module, a vulnerability repair module, a vulnerability database, a virus scanning module, a virus database, a virus isolation module, a risk assessment module, a network security monitoring module, a data migration module, an isolation backup module, a data encryption module and an encryption database. The central processing unit is in communication connection with the identity verification module, the display module, the vulnerability detection module and the virus scanning module; the vulnerability detection module is in communication connection with the vulnerability database and the vulnerability repair module, and the virus scanning module is in communication connection with the virus database and the virus isolation module. According to the method, vulnerability and viruses can be rapidly detected and processed, Trojan horse viruses are effectively prevented from invading an industrial system, data in the industrial system can be effectively protected, data loss and stealing are prevented, the safety performance of the industrial system is remarkably improved, the use effect is extremely good, and the system is suitable for application and popularization.

The invention discloses a system and method for carrying out virus insulation on monitored applications. The system comprises a setting unit which is used for setting a security level of each security function of user equipment and setting a risk level for each monitored application in a virus insulation area; a monitoring unit which is used for monitoring operation of the monitored applications in the virus insulation area and sending an access request to a control unit rather than sending the access request to a first security function when a first application initiates the access request for the first security function in the plurality of security functions; the control unit which is used for determining a response strategy capable of ensuring information security according to the security level of the first security function involved to the access request and the risk level of the first application, in response to the access request and generating a response message according to the response strategy; and a response unit which is used for answering the access request of the first application through utilization of the response message.

Foot and out disease virus (FMDV) is worldwide problem. Rapid isolation, serotyping and vaccine matching of FMDV from infected animals is critical to enable the implementation of effective vaccination programs and to stop the spread of infection during outbreaks. Current virus isolation protocols use primary cells, known to be susceptible to FMDV, or baby hamster kidney cells (BHK-21) and other cell lines that are not highly sensitive to some strains of FMDV. The αVβ6 integrin is a principal receptor for FMDV. We therefore transduced the porcine kidney cell line, LFBK, to stably express both the αV and β6 bovine integrin subunits. The LFBK-αVβ6 cell line showed both β6 expression and enhanced susceptibility to FMDV infection for at least 100 cell passages. LFBK-αVβ6 cells are highly sensitive for detecting all serotypes of FMDV from experimentally infected animals, including the porcinophillic strain O / TAW / 97 and are thus a sensitive tool for FMDV isolation.

The invention belongs to the technical field of masks, and particularly relates to a virus isolation type mask. At present, various masks, including medical masks, N95 masks and various electric masks, cannot completely and effectively isolate virus infection. The invention aims to provide a mask capable of effectively isolating viruses. According to the mask, a functional structure for killing viruses through ultraviolet rays is additionally arranged in an air filtering system of the mask, so that viruses including novel coronavirus can be instantly inactivated, a wearer can normally work and freely move without virus infection, and the mask is suitable for preventing virus infection when public plague occurs. The mask can filter particles and harmful gas in air, and a user can use the mask to prevent air pollution.

The invention relates to isolation-level infectious disease protection underpants which comprise underpants, a trouser waist telescopic belt, trouser leg telescopic belts and an exhaust port and are made of rubber materials with good sealing performance. At present, viruses are found in excrement of most infectious disease virus carriers, such as SARS virus and new coronapneumonia virus. The excrement of a virus carrier is proved to be capable of transmitting viruses; a virus carrier can expel viruses from the anus, so that viruses can invade normal people from the anus to cause infection, theisolation-level infectious disease protection underpants capable of completely isolating the navel eyes, the anus and the external genitals are invented, a virus carrier can isolate viruses and can prevent the viruses from spreading outwards to infect others when wearing the isolation-level infectious disease protection underpants, and normal people can prevent the viruses from invading bodies from the navel eyes, the anus and the external genitals when wearing the isolation-level infectious disease protection underpants, thereby achieving infectious disease isolation and protection.

The invention discloses an anti-virus E-mail processing system and method. The anti-virus E-mail processing system comprises a virus detecting unit, a virus isolating unit and a virus processing unit, wherein the virus detecting unit is installed on a computer; the virus isolating unit is used for receiving virus detected information uploaded by the virus detecting unit and isolating the virus; the virus processing unit is used for receiving the virus detected information uploaded by the virus detecting unit and eliminating the virus. The anti-virus E-mail processing method comprises the following steps: a) detecting if the virus exists: utilizing a virus detecting module to detect if the virus exists in the E-mail when the E-mail is received by the computer; b) isolating the virus: refusing to open the E-mail and utilizing a virus isolating module of the computer to isolate the virus when the virus is detected; c) processing the virus: utilizing an antivirus program on the computer to perform anti-virus treatment on the E-mail.

The invention discloses a virus isolation method for a low-content sample of porcine epidemic diarrhea viruses (PEDV), and further discloses an immune nano-magnetic bead for enrichment and isolation of the PEDV. The immune nano-magnetic bead has a magnetic Fe2O3 core and a surface coupled with PEDV membrane protein-specific single-domain antibodies. Traditional methods for isolating viruses from multiple infected and low PEDV-content samples require viral purification and long-term blind transmission, the separation efficiency is low, and the research work of the PEDV is limited. The virus isolation method, provided by the invention, utilizes nano-magnetic beads and PEDV-specific single-domain antibodies to prepare the immune nano-magnetic beads, and can effectively capture and enrich thePEDV in various samples such as feces and tissues. After the samples are processed, the immune nano-magnetic beads are utilized for virus capture and enrichment, the immune nano-magnetic beads combined with the virus are directly inoculated into Vero cells, and the virus isolation can be achieved. The immune nano-magnetic beads and the PEDV isolation method prepared by the invention are suitable for rapid and effective isolation of the PEDV in clinically complex and low virus-content samples.

The present invention discloses an HRM primer set for identifying canine parvovirus (CPV) and feline parvovirus (FPV), a kit containing the primer set, and applications of the kit, wherein the primerset comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer is represented by SEQ ID NO.1, and the nucleotide sequence of the downstream primer is represented by SEQ ID NO.2. The present invention further provides an HRM analysis kit containing the primer set. According to the present invention, the experiment results prove that the kit has good sensitivity and good specificity in the detection of FPV and CPV, and has high coincidence, high relative specificity and high sensitivity compared to virus isolation, DNA sequencing and other tests; and thekit can provide the effective technical means for the prevention and control of diseases caused by canine parvovirus (CPV) and feline parvovirus (FPV).