21 results about "Virus isolation" patented technology

The invention discloses a separation method of a bovine ephemeral fever virus, the obtained bovine ephemeral fever virus and application of the bovine ephemeral fever virus. Belongs to the technical field of bovine ephemeral fever virus separation. The invention aims to provide an efficient and rapid method for separating the bovine ephemeral fever virus. The invention provides a method for separating bovine ephemeral fever virus, which comprises the following steps: inoculating a bovine anticoagulant sample to KC cells for culture, harvesting virus, inoculating to grow into monolayer BHK-21 cells, and carrying out passage to obtain passage cells with cytopathy of the first generation; the virus strain can also be used for screening, preparing and detecting a bovine ephemeral fever virus antibody and preparing a bovine ephemeral fever virus infected animal model.

The application discloses an isolation method of bovine epidemic fever virus and the bovine epidemic fever virus obtained by the method and application of the bovine epidemic fever virus, and belongs to the technical field of bovine epidemic fever virus isolation. The application aims to provide an efficient and rapid method for isolating bovine epidemic fever virus. The application provides the isolation method of the bovine epidemic fever virus, wherein the anticoagulated blood sample of a bovine is inoculated on KC cells for culture, the virus is harvested, the BHK-21 cells are inoculated to grow into a monolayer, and the first generation of passage cells with cytopathic effect is obtained by passage. The virus strain can also be used for screening, preparation and detection of bovine epidemic fever virus antibodies, and for preparation of an animal model infected by the bovine epidemic fever virus.

The utility model discloses an animal epidemic disease virus isolation device, which belongs to the technical field of animal epidemic diseases and comprises an isolation box, two sides of the isolation box are connected with stabilizing components, the bottom surface of the isolation box is connected with a moving component, and a filter plate is connected in the isolation box. The isolation device can be easily moved to the vicinity of an animal needing to be isolated, the working efficiency is improved, the practicability and flexibility of the device are enhanced, after the isolation device is moved to a proper position, the stability of the isolation device can be improved through the stabilizing assembly, and the filter plate is used for supporting the sick animal; meanwhile, excrement and excrement of animals are allowed to penetrate and fall into a collection box below, physical separation of the animals and the excrement is achieved, subsequent harmless treatment of the excrement is facilitated, and transmission of viruses through the excrement path is effectively blocked; and a worker can easily detach the device for cleaning, disinfection or replacement when needed.

The invention relates to the technical field of biological detection, and discloses a composition and a kit for detecting a virus of positive bunyavirus and application of the composition and the kit. The composition disclosed by the invention can be used for rapidly and simultaneously detecting the oropercut virus, the Batai virus and the Akabai virus in a sample in a high-specificity manner, and has relatively high sensitivity and stability. Compared with traditional detection methods such as virus separation, genome sequencing and the like, the efficiency of identifying and detecting the oropercut virus, the Batai virus and the Acamovirus by adopting the composition disclosed by the invention is effectively improved, the detection process is simple and convenient to operate, and the composition is suitable for large-scale popularization and application.

This application discloses a circular RNA vaccine against porcine reproductive and respiratory syndrome (PRRS) virus, its construction method, and its application. The circular RNA vaccine comprises circular RNA and a pharmaceutically acceptable carrier. The coding elements in the circular RNA encode the GP4, GP2, GP5, and M proteins of PRRS virus. The circular RNA vaccine exhibits high stability and good safety, producing no adverse reactions after immunization of pigs. It induces high levels of neutralizing antibodies in pigs after immunization and activates a high level of cellular immune response, providing up to 100% immunoprotection. Furthermore, its preparation process eliminates the need for virus isolation, significantly shortening the research and development cycle. The production process is not constrained by biological factors such as virus titer, facilitating standardization and large-scale production, and effectively reducing production costs.

The invention discloses a pelteobagrus fulvidraco brain tissue cell line PYCB sensitive to viruses and application thereof, and belongs to the technical field of aquatic organism cell culture and disease prevention and control. The preservation number of the Pelteobagrus fulvidraco brain tissue cell line PYCB is CCTCC (China Center For Type Culture Collection) NO: C2025148. The Pelteobagrus fulvidraco brain tissue cell line PYCB is successfully constructed by taking the Pelteobagrus fulvidraco brain tissue as an object, and the cell line can be continuously and stably subcultured, is high in proliferation speed and simple in culture method, is sensitive to Pelteobagrus fulvidraco small RNA viruses and Pelteobagrus fulvidraco stem casing viruses, and can be used for isolated culture of viruses. The invention provides an important research platform and an experimental material for separating and identifying fish viruses and virus vaccines and developing medicines.

The application belongs to the technical field of animal cell engineering, and particularly relates to a pigeon fibroblast cell strain X4 and application thereof. A pigeon fibroblast cell strain is provided, which is named pigeon fibroblast cell X4, and the preservation number is CGMCC No. 46754. The pigeon fibroblast cell strain can be used for virus isolation or amplification, virus pathogenic mechanism research, and development of antiviral drugs and vaccines, and has a good application prospect in pigeon disease research.

The invention belongs to the technical field of animal cell engineering, and particularly relates to a pigeon fibroblast strain X4 and application thereof. The invention provides a pigeon fibroblast strain which is named as pigeon fibroblast X4 and has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 46754. The pigeon fibroblast cell strain can be used for separation or amplification of viruses, research of pathogenic mechanisms of the viruses and development of antiviral drugs and vaccines, and has a good application prospect in pigeon disease research.

The invention relates to a bioengineering technology, and aims to provide an IPEC-J2 cell strain for stably expressing a PED virus nucleocapsid protein truncated fragment and application of the IPEC-J2 cell strain. The cell strain is preserved in the China Center for Type Culture Collection, the preservation name is a porcine small intestine epithelial cell line R58-WT-IPEC, the Latin name is Sus scrofa, and the preservation number is CCTCC NO: C202425. The invention discloses a key effect of the PEDV N protein in a virus replication stage, and provides application of the PEDV N protein as a target spot in regulation and control of PEDV infection. Compared with a wild-type IPEC-J2 cell line, the cell line provided by the invention has a remarkable promotion effect, can solve the problem of poor proliferation of PEDV (porcine epidemic diarrhea virus) in the IPEC-J2 cell line, and further improves the application possibility of the IPEC-J2 cell line in virus separation and propagation.

The invention discloses a method for separating porcine reproductive and respiratory syndrome virus and porcine circovirus, and belongs to the technical field of virus separation. The separation method comprises the following steps: preparing a virus positive sample into homogenate, and centrifuging to obtain virus liquid; mixing the virus solution with polypropylene glycol and a cholesterol solution to obtain a virus infection solution; inoculating cells with the virus infection solution, and co-culturing in a virus culture solution; and after co-culture is finished, freezing and thawing the cell culture for three times to serve as an F1 generation, subculturing to an F3 generation according to the steps (1)-(3), and harvesting venom. According to the method, polypropylene glycol and cholesterol are added in the virus separation process, the sensitivity of cells to viruses is improved, noradrenaline is added into a virus culture solution, a cell signal channel is adjusted, virus proliferation and release can be promoted, and the purpose of rapidly separating the viruses is achieved.

This invention relates to the field of virus isolation and purification technology, and discloses a method for efficiently enriching viruses using polyglutamic acid in synergistic effect with calcium ions. The method includes: preparing a polyglutamic acid mother liquor, a calcium ion mother liquor, and a chelating agent working solution; adding the polyglutamic acid mother liquor and the calcium ion mother liquor sequentially to the virus lysis buffer and adjusting the pH to obtain a mixed solution; centrifuging after static sedimentation to obtain a wet precipitate; dissolving the wet precipitate in the chelating agent working solution, diluting with PBS buffer, purifying by ultrafiltration, washing, and collecting the retentate; and adding sucrose and bovine serum albumin to obtain the enriched virus solution. This invention, by sequentially adding high molecular weight polyglutamic acid mother liquor and calcium ion mother liquor to the virus lysis buffer, utilizes the coordination cross-linking effect formed between calcium ions and polyglutamic acid on the virus surface to capture dispersed virus particles and form flocculated precipitates, thereby shortening the sedimentation time and improving the virus recovery rate.

The application discloses a Plectropomus leopardus spleen cell line and application thereof. Plectropomus leopardus The spleen cell line PLS has a preservation number of GDMCC No: 68131. The PLS is obtained through primary culture and multiple subculture, has a fast cell growth speed, has been stably subcultured for more than 60 generations, has good activity after cryopreservation, and lays a foundation for Plectropomus leopardus gene germplasm resource preservation. Meanwhile, the PLS is sensitive to fish nervous necrosis virus and iridovirus, can be applied to exogenous gene function research, fish virus isolation, virus infection pathogenesis, virus host interaction and virus in-vitro infection model, and provides a high-efficiency amplification system for Plectropomus leopardus virus vaccine preparation.

The application discloses a micro-fluidic cell chip and a virus separation and culture method based on the cell chip. The micro-fluidic cell chip comprises a metal frame, a lower cover plate, a chip frame, a core chip and an upper cover plate, the core chip is provided with a plurality of cell culture cavities, a main flow channel is arranged on the surface of the core chip, and the main flow channel is communicated with the plurality of cell culture cavities. The virus separation and culture method based on the micro-fluidic cell chip adopts a plurality of cell lines for co-culture, realizes the sequential incubation of one sample to be analyzed and different cell lines, can significantly save clinical samples, maximally improves sample utilization, simultaneously reduces the consumption of culture reagents, shortens the separation and identification time, and theoretically realizes the culture of at least ten different cell lines at the same time due to the design of the micro-fluidic cell chip provided by the application, significantly increases the throughput of virus sensitive host cell screening, and realizes the high-throughput, automatic and rapid screening of virus sensitive cell lines.

There is provided a method of separating a smaller virus from a larger virus from a fluid, the method comprising (a) passing the fluid containing the smaller virus and the larger virus through a porous filter comprising a porous medium and obtaining the smaller virus at a differential pressure of at least 20 psid; (b) reducing the pressure to 3 psid or less; and (c) passing an additional virus-containing fluid through the porous filter comprising the porous medium at a pressure of at least 20 psid and obtaining an additional smaller virus.

This invention discloses a method for efficiently isolating human parainfluenza virus based on suspended MDCK cells. By using the fully suspended passaged MDCK cell line for human parainfluenza virus isolation and culture and optimizing the inoculation process, single-generation virus amplification can be completed within 3 days, significantly shortening the cycle, improving virus isolation efficiency and success rate compared to traditional adherent culture methods, and enhancing experimental reproducibility. This method not only demonstrates good isolation and culture capabilities for the major subtypes of human parainfluenza virus, but also shows good adaptability to clinical samples from different sources and with different initial loads. In addition, this culture system has linear scale-up potential, is not limited by the surface area of ​​adherent culture, and can meet the large-scale virus seed preparation needs from clinical testing to vaccine mass production, providing key technical support for the integrated process from human parainfluenza virus isolation to vaccine production.

An image forming apparatus includes a setting unit that performs setting of a system, an image formation section that forms an image according to the setting, and a processing unit that performs a process for a quarantine against a virus. The setting unit performs setting concerning security. The processing unit implements the quarantine against a virus if the setting concerning security has been changed.

A method for separating smaller viruses from larger viruses from a fluid is provided, comprising the steps of: (a) passing a fluid containing the smaller viruses and the larger viruses through a porous filter comprising a porous medium at a differential pressure of at least 20 psid to obtain the smaller viruses; (b) reducing the pressure to 3 psid or less; and (c) passing additional fluid containing the viruses through the porous filter comprising the porous medium at a pressure of at least 20 psid to obtain additional smaller viruses.

The invention discloses a separation method for separating duck circovirus by using SPF duck embryos, belongs to the technical field of biological vaccine prevention and treatment, and solves the technical problem that in the prior art, the duck circovirus does not successfully obtain a virus breeding method. The separation method for separating the duck circovirus by using the SPF duck embryo, provided by the invention, comprises a duck circovirus inoculation mode, a virus inoculation method and a method for obtaining a virus culture. The virus separation method is easy and convenient to operate, economical, suitable and small in potential infection risk, and can be used as a common research method for duck circovirus separation, identification, antigen preparation and the like. So far, the method is of great significance to research on the pathogenesis and pathogenesis of the immunosuppressive disease caused by the duck circovirus.

An image forming apparatus includes a provider that provides a setting screen on which settings of a system are executed; a setter that executes settings of the image forming apparatus, based on a content set on the setting screen; an image former that forms an image according to the set content; and a processor that executes virus quarantine processing. The provider provides the setting screen including settings pertaining to a virus quarantine timing.

The present application relates to a positive pressure isolation dust and virus isolation power air supply system, belonging to the technical field of protective equipment. The system comprises a protective head cover, a fan-shaped air pipe, a gas conveying hose, a quick pipe joint and a power air blower. The fan-shaped air pipe is arranged on the inner side of the protective head cover. One end of the fan-shaped air pipe extends to the front of the inner side of the protective head cover, and the other end of the fan-shaped air pipe extends to the back of the inner side of the protective head cover. The gas conveying hose is connected to the fan-shaped air pipe at the back of the inner side of the protective head cover through the quick pipe joint. The gas conveying hose is in communication with the protective head cover. The other end of the gas conveying hose is connected to the power air blower through a blower connecting head. The power air blower is in communication with the gas conveying hose. The system can deliver clean air to the face of the wearer through the gas conveying hose by the power air blower, so as to quickly take away the hot air exhaled by the wearer and prevent the perspective window on the air-permeable cloth cover from fogging. The system can be assembled by inserting the connecting elbow into the lock tongue sleeve, tightening the lock tongue ring and shortening the assembly time.

The invention discloses separation, identification and application of a porcine bocavirus cell adapted strain, and belongs to the technical field of gene engineering. The preservation number is CGMCC (China General Microbiological Culture Collection Center) No.46413, and the strain can be stably proliferated in passage cells of porcine kidney cells (PK) and can induce a remarkable cytopathic effect. Virus separation and identification are completed through metagenome sequencing, transmission electron microscope observation and indirect immunofluorescence technologies, and a phylogenetic tree is constructed based on a VP1 gene sequence. The invention also establishes a piglet infection model, and proves that the porcine bocavirus cell adapted strain CNH-2024 can cause acute diarrhea and lung pathological injury. The invention also provides a specific fluorescent quantitative PCR primer combination, a vaccine preparation method and a diagnostic kit. The porcine bocavirus cell adapted strain CNH-2024 obtained by separation and identification provided by the invention provides an important technical basis for porcine bocavirus pathogenic mechanism research, vaccine development and clinical diagnosis.