29 results about "Cell chip" patented technology

The application provides a preparation method of organoids based on a single cell chip, which comprises microfluidic chip preparation, a two-water-phase solution preparation, a single cell suspension preparation, microfluidic manipulation, single cell-loaded microgel generation and organoid construction and the like. The application takes a microfluidic chip integrated with a normally closed pneumatic pump valve as a technical platform, takes a single cell suspension pre-incubated with a crosslinking agent as a chemical reaction core, takes a water-in-water droplet as a forming template, and realizes efficient in-situ loading of single dispersed seed cells in a micro hydrogel carrier through a one-step method, and simply removes empty droplets without cells in a subsequent transfer step. The single cells in the microgel can be cultured, expanded and self-organized to form required organoids. The application improves the consistency of the starting point of the organoids, has the high-throughput characteristics of the droplet microfluidic technology, realizes efficient and highly reproducible in-vitro construction of the organoids, and can play a great role in the fields of basic research and transformation application related to the organoids.

Disclosed herein is cell culturing device, cell culturing systems, methods to produce a cell culturing device, a kit or parts, methods for culturing cells, cellular chips, the use of such a cellular chip, a cell culturing device blank and a cell culturing blank system.

A disclosed cell chip comprises: a substrate on which a plurality of electrode pads are provided in pairs; a bonding wire which connects each pair of electrode pads in an arch shape having a height so that the electrode pads are electrically connected; and a resin layer stacked on the substrate such that a portion including the apex of the bonding wire is exposed.

A memory device including a memory block performing a sub-block operation is provided. The memory device includes a chip stack structure, which includes a peripheral circuit structure and a cell array structure. The cell array structure includes a first cell chip and a second cell chip on the first cell chip. The first cell chip includes a plurality of first sub-blocks, and the second cell chip includes a plurality of second sub-blocks. Each of the memory blocks is constituted of a first sub-block and a second sub-block corresponding thereto. The first word lines are respectively connected to the second word lines. In an erase operation of a selected first sub-block of a selected memory block among the memory blocks, the first string select lines sequentially float with a delay at a level of a ground voltage starting from a first string select line far from the first bit lines.

A cell chip is produced by performing a fixing step for immersing cells in a cell fixing liquid, an arrangement step for discharging a cell suspension containing the fixed cells and a liquid from an inkjet-mode discharge part onto a discharge target medium such as a membrane to arrange droplets of the cell suspension on the discharge target medium, and a dyeing step for dyeing a cell-derived component, in this order.

A method of manufacturing a semiconductor device includes forming a cell chip including a first substrate, a source layer on the first substrate, a stacked structure on the source layer, and a channel layer passing through the stacked structure and coupled to the source layer, flipping the cell chip, exposing a rear surface of the source layer by removing the first substrate from the cell chip, performing surface treatment on the rear surface of the source layer to reduce a resistance of the source layer, forming a peripheral circuit chip including a second substrate and a circuit on the second substrate, and bonding the cell chip including the source layer with a reduced resistance to the peripheral circuit chip.

The application discloses a cell culture chip and a single-cycle fluid device, wherein the cell culture chip comprises a base, and the base is provided with a plurality of liquid storage chambers used for storing culture solution, each of the liquid storage chambers is provided with an outlet and an inlet, the outlet only allows the culture solution to flow out, and the inlet only allows the culture solution to flow in, at least one culture groove is arranged between each pair of the liquid storage chambers, and the culture groove is used for culturing a cell chip; in each pair of the liquid storage chambers, the outlet of one of the liquid storage chambers is communicated with the inlet of the other liquid storage chamber through the at least one culture groove, and the inlet of the one liquid storage chamber is communicated with the outlet of the other liquid storage chamber. By adopting the two single-flow liquid storage chambers, the culture solution in the two liquid storage chambers can only flow out from the outlet and flow in from the inlet, so that the unidirectional flow of the culture solution in the culture groove is realized, and the culture solution in the two liquid storage chambers can also be circulated.

The embodiment of the present application relates to the technical field of cell chip, and discloses a single cell antibody chip, which is characterized by comprising: a chip substrate, wherein a biological recognition area and a physical function area are arranged on the chip substrate; a first group of core points is arranged in the biological recognition area, the first group of core points comprises a plurality of biological recognition core points, each biological recognition core point is pre-coated with different markers for capturing and detecting a to-be-detected substance; a second group of core points is arranged in the physical function area, the second group of core points comprises a plurality of physical function core points, the physical function core points comprise first positioning core points and second positioning core points, and the display states of the first positioning core points and the second positioning core points are different. In the embodiment of the present application, the single cell antibody chip can capture and label single cells, and the chip substrate can be directionally identified by using the positioning core points, and the imaging result can be uniformly processed by using the quantitative core point array, so that the expression level of the protein can be better quantitatively detected.

The invention discloses a solar thin-film cell with mildew-proof and hydrophobic functions and a preparation method thereof, and relates to the technical field of solar cells. The solar thin-film cell comprises a thin-film cell chip, an adhesive film, front plate glass and a hydrophobic coating, the hydrophobic coating comprises organic silicon acrylic resin, lignosulfonate and chromium trichloride; the thin film battery chip, the adhesive film and the front plate glass are stacked layer by layer to form a battery substrate, a hydrophobic coating on the surface of the front plate glass contains organic silicon acrylic resin, lignosulfonate and chromium trichloride, specifically, the organic silicon acrylic resin provides basic hydrophobicity, flexibility and weather resistance, and the hydrophobic coating can be used for coating the thin film battery chip, the lignosulfonate and the chromium trichloride. The water vapor can form a high contact angle on the water vapor and is easy to tumble, so that the surface of the solar thin-film battery obtains water repellency, dryness and cleanness of the surface of the solar thin-film battery can be effectively ensured, mildew of the surface of a component caused by overhigh humidity is avoided, and the stability of the performance of the solar battery is ensured. The power attenuation speed of the solar cell is slowed down and the power generation efficiency is improved.

The present disclosure provides a 3D organoid analysis cell chip adapted to perform non-destructive electrical analysis on a 3D organoid in real time, whereby the chip allows for multiple experiments through safe handling of organoids and can acquire research outcomes such as real-time drug response monitoring, evaluation of growth depending on size changes, metabolic analysis, etc., more rapidly compared to conventional technologies, through non-destructive real-time electrical analysis, reduce variables for failure risk factors during culture and analysis, and find technical applications in various technical fields with high measurement sensitivity.

The utility model discloses an annealing device for a photovoltaic cell chip assembly, the annealing device comprises a heating table and a moving part, a heating surface is formed on the heating table, an open heating hole is formed in the heating surface, the heating hole is suitable for outputting a heating medium and heating the chip assembly passing through the heating surface, the moving part can move relative to the heating table, and the moving part can move relative to the heating table. The moving part is suitable for driving the chip assembly to suspend and move relative to the heating surface, so that the annealing uniformity of the annealing device can be improved.

A cell chip is provided that exhibits excellent resistance to cell desiccation, cell retention, and detection of chemical substances such as odorants. The cell chip comprises a compartment containing a cell and a gel that can be converted into a sol.

The present invention relates to a cell chip for measuring local field potential (LFP), the chip comprising: an electrode array in which a reference electrode and a plurality of working electrodes are spaced apart from each other; transmission lines connected to respective electrodes; and a terminal pad part, wherein the surface of each working electrode is modified with a conductive nanomaterial to reduce interfacial impedance and improve sensitivity to electrical signals.

The invention relates to the field of photovoltaic cell preparation, in particular to a preparation method of a thin-film solar cell, which comprises the following steps: preparing a power generation layer on a substrate to obtain a cell chip; removing a certain width of the edge of the power generation layer; leading out the bus bar from the power generation layer; a rear packaging adhesive film and a back plate are sequentially placed on the side, away from the substrate, of the power generation layer in sequence and are aligned, and a stacked assembly is obtained; laminating the laminated assembly to obtain a laminated assembly; cutting the laminated assembly; and installing a junction box on the cut laminated assembly and communicating the junction box with the bus bar, and then testing. The cutting step is carried out after the laminating step, it is ensured that the fragile power generation layer is protected at the beginning of machining, pollution and damage to the power generation layer caused by follow-up procedures are completely eradicated, and the power generation layer can be prevented from being exposed to the external environment in the cutting process; the technical problems that the power generation layer is easily polluted during cutting and the power generation layer is easily damaged during mechanical cutting are fundamentally solved, so that the product yield and the long-term reliability are improved.

A detection technology is provided with excellent resistance to cell desiccation, cell retention, and the detection of chemical substances such as odorants. The system comprises a layered construct containing a cell layer and a gel layer placed on top of the cell layer, as well as a cell chip containing the layered construct.

A memory device comprises a cell chip including a memory cell array having a plurality of first lines and a plurality of second lines, first and second bonding pads adjacent to each other in a first region and electrically connected to the plurality of first lines, and third and fourth bonding pads adjacent to each other in a second region different from the first region and electrically connected to the plurality of second lines, and a logic chip electrically connected to the first, second, third, and fourth bonding pads of the cell chip. The first and second bonding pads are electrically connected to a third line among the plurality of first lines, and the third and fourth bonding pads are electrically connected to fourth and fifth lines among the plurality of second lines, respectively.

Memory devices are provided that include memory blocks that perform sub-block operations. The memory devices include a chip stack that includes a peripheral circuit structure and a cell array structure. The cell array structure includes a first cell chip and a second cell chip on the first cell chip. The first cell chip includes a plurality of first sub-blocks and the second cell chip includes a plurality of second sub-blocks. Each of the memory blocks is made up of a first sub-block and a corresponding second sub-block thereof. First word lines are connected to second word lines, respectively. In an erase operation of a selected first sub-block of a selected memory block among the memory blocks, first string select lines starting from a first bit line away from the first string select lines are sequentially floated with a delay at a level of a ground voltage.

The application discloses a micro-fluidic cell chip and a virus separation and culture method based on the cell chip. The micro-fluidic cell chip comprises a metal frame, a lower cover plate, a chip frame, a core chip and an upper cover plate, the core chip is provided with a plurality of cell culture cavities, a main flow channel is arranged on the surface of the core chip, and the main flow channel is communicated with the plurality of cell culture cavities. The virus separation and culture method based on the micro-fluidic cell chip adopts a plurality of cell lines for co-culture, realizes the sequential incubation of one sample to be analyzed and different cell lines, can significantly save clinical samples, maximally improves sample utilization, simultaneously reduces the consumption of culture reagents, shortens the separation and identification time, and theoretically realizes the culture of at least ten different cell lines at the same time due to the design of the micro-fluidic cell chip provided by the application, significantly increases the throughput of virus sensitive host cell screening, and realizes the high-throughput, automatic and rapid screening of virus sensitive cell lines.

The utility model discloses a device for detecting electrical impedance of a cell layer. The device for detecting the electrical impedance of the cell layer comprises a base, a chip to be detected, a top plate and an external plug, the base is provided with a lower circuit board; the chip to be detected consists of an upper glass electrode, a lower glass electrode and a cell chip body positioned between the upper glass electrode and the lower glass electrode; the top plate is provided with an upper circuit board; according to the utility model, the glass electrode for measuring the electrical impedance of the cell layer is stably and reliably connected to an external measuring circuit through a unique measuring loop design and a circuit connection mode, so that the device has stable measuring signals and strong anti-interference capability.

A method of manufacturing a semiconductor device includes forming a cell chip including a first substrate, a source layer on the first substrate, a stacked structure on the source layer, and a channel layer passing through the stacked structure and coupled to the source layer, flipping the cell chip, exposing a rear surface of the source layer by removing the first substrate from the cell chip, performing surface treatment on the rear surface of the source layer to reduce a resistance of the source layer, forming a peripheral circuit chip including a second substrate and a circuit on the second substrate, and bonding the cell chip including the source layer with a reduced resistance to the peripheral circuit chip.

Provided is a technology capable of improving the detectability of chemical substances such as odor substances when cells are cryopreserved. A cell chip containing cells for cryopreservation with recovery culture.

The invention relates to the technical field of solar cells, in particular to a three-junction solar cell capable of adjusting stress and a preparation method thereof, and the three-junction solar cell sequentially comprises a GaAs substrate, a corrosion cut-off layer, a contact layer, a top cell, a first tunnel junction, a middle cell, a second tunnel junction, a strain buffer layer, a bottom cell, a stress adjusting layer and an ohmic contact layer from bottom to top. By growing the stress adjusting layer on the bottom cell layer, not only can the compressive strain of the bottom cell layer on the front two cell layers and the substrate be compensated, but also the lattice constant and the thickness of the functional layer can be freely adjusted to compensate the stress generated by mismatch under the condition that the structure of the functional layer is not influenced, the warping of the epitaxial wafer is adjusted, and the reliability of the epitaxial wafer is improved. And the production yield and the device reliability of the flip cell chip can be improved.

The application discloses a kind of based on microfluidic technology's single cell ChIP-seq library construction method.The construction method uses the Tn5 mutant described in the application, the fusion protein described in the application, the linker combination described in the application or the kit described in the application to construct single cell ChIP-seq library.In the library construction method of the application, all single cells are collected in a tube to carry out one-tube operation, operation is simple, physical labor is reduced, and the time of library preparation is shortened;And microfluidic technology reduces the difficulty of single cell acquisition, and can easily realize high throughput;In addition, the specific activity of ProteinX-Tn5 is improved, and the cell contamination rate can be as low as 2% or less;Through the combination of microfluidic technology and ChIP-seq technology, the original tube is used to build a library, and the capture rate of DNA fragments is guaranteed.

This invention discloses a flexible solar panel battery chip bonding material for satellites and its preparation method, relating to the field of chip bonding material technology. The flexible solar panel battery chip bonding material for satellites is composed of component A and component B mixed in a mass ratio of 1:(0.05-0.1). Component A includes the following raw materials in parts by weight: 100 parts of special resin, 5-8 parts of diluent, 30-40 parts of modified functional filler, 0.1-0.5 parts of defoamer, and 0.1-0.5 parts of leveling agent. Component B includes the following raw materials in parts by weight: 50-70 parts of polysilazane, 20-30 parts of crosslinking agent, and 0.1-2 parts of catalyst. The flexible solar panel battery chip bonding material for satellites prepared by this invention exhibits excellent hardness, tensile strength, elongation at break, shear strength, and thermal conductivity.

Provided is a detection technique having excellent cell dryness resistance, cell retention, and detectability of chemical substances such as odor substances. The present invention provides: a laminate comprising a cell layer and a gel layer disposed on the cell layer; and a cell chip comprising the laminate.

Provided is a cell chip having excellent cell dryness resistance, cell retention, and detectability of chemical substances such as odor substances. A cell chip comprises a partition comprising cells and a gel that can be solated.

The present application relates to the technical field of single cell analysis, and particularly relates to a microfluidic single cell chip and a gas-liquid exposure particulate matter toxicity analysis method, the chip comprising a liquid inlet, a gas inlet, a plurality of parallel single cell capture arrays, the plurality of single cell capture arrays sharing an outlet, the single cell capture array comprising a serpentine main channel, a plurality of capture trap-cavity units being arranged between two adjacent rows of segments, the capture trap-cavity unit comprising a cell capture trap, a front limiting channel and a cell culture cavity in communication, the inlet of the cell capture trap being in communication with the last row of segments, the middle of the cell culture cavity being in communication with the rear limiting channel, and the rear limiting channel being in communication with the next row of segments. The present application adopts the method of parallel capture trap-cavity units to increase the number of capture-cavity units per unit area, ensuring that the chip has a high enough cell capture amount; and ensuring that the cells are still immersed in the culture solution during the gas-liquid exposure process, meeting the application of particulate matter toxicity analysis.

The invention provides an artificial intelligence expert system and method for cancer drug screening based on a cell chip. The artificial intelligence expert system and method comprises the following steps: acquiring first micro-fluidic chip experimental data, reference drug molecule data of reference drug components, and one or more reference drug component directories of the reference drug components; and performing drug feature vector analysis processing on the first micro-fluidic chip experimental data to obtain a first visual recommendation result of the cell reaction feature vector set. Obtaining one or more first cell reaction feature vector set directories of the cell reaction feature vector set; and obtaining an intelligent screening result of the cancer drugs according to the similarity between the first visual recommendation result and the reference drug molecule data and the matching condition of the one or more first cell reaction feature vector set directories and the one or more reference drug component directories. The problems that traditional screening is low in efficiency, insufficient in precision and poor in interpretability are solved, and efficient and intelligent technical support is provided for research and development of anti-cancer drugs.

The invention relates to the technical field of biological detection, and provides a replaceable cell chip based on a bionic self-circulation microenvironment and an electrochemical in-situ monitoring method. The cell chip comprises a chip main body formed by curing polydimethylsiloxane, and a detection chamber and an electrode accommodating chamber are vertically distributed in the chip main body in a laminated manner; a detection electrode containing a cytoskeleton is arranged in the pluggable electrode plug assembly, and a guide groove design is adopted in the plugging process, so that stable contact and sealing performance of the electrode and a chamber interface are ensured; the double-liquid self-circulation micro-channel system is connected with the chip body through a liquid inlet and a liquid outlet, and a peristaltic pump is adopted to drive double-liquid circulation to realize synchronous cell culture and detection. The service life of the chip is remarkably prolonged through the replaceable design of the electrode plug, and the cell activity state and the drug response characteristic can be continuously monitored under the condition that the culture process does not need to be interrupted in combination with the real-time sensing function of the detection cavity and the dynamic microenvironment regulation and control of the double-liquid circulation system.