248 results about "Mdck cell" patented technology

The present invention provides novel MDCK-derived adherent non-tumorigenic cell lines that can be grown in the presence or absence of serum. The cell lines of the present invention are useful for the production of vaccine material (e.g., viruses). More specifically, the cell lines of the present invention are useful for the production of influenza viruses in general and ca / ts influenza viruses in particular. The invention further provides methods and media formulations for the adaptation and cultivation of MDCK cells such that they remain non-tumorigenic. Additionally, the present invention provides methods for the production of vaccine material (e.g., influenza virus) in the novel cell lines of the invention.

The invention provides a composition useful to prepare high titer influenza viruses, e.g., in the absence of helper virus, which includes internal genes from an influenza virus vaccine strain or isolate, e.g., one that is safe in humans, for instance, one that does not result in significant disease, that confer enhanced growth in cells in culture, such as MDCK cells, or in eggs.

The present invention relates to novel MDCK cells which can be to grow viruses, e.g., influenza viruses, in cell culture to higher titer than previously possible. The MDCK cells can be adapted to serum-free culture medium. The present invention further relates to cell culture compositions comprising the MDCK cells and cultivation methods for growing the MDCK cells. The present invention further relates to methods for producing influenza viruses in cell culture using the MDCK cells of the invention.

The invention discloses a serum-free medium for full-suspension culture of MDCK cells (madin-darby canine kidney cells) and a preparation method of the serum-free medium. The serum-free medium for full-suspension culture of the MDCK cells consists of a basic metabolism nutrient, nucleotide, vitamins, inorganic salt, a shear force protective agent, a cell clustering resisting agent, a pH buffer agent, a pH indicator, an influenza virus proliferation accelerant and other additives; and the preparation method of the serum-free medium for full-suspension culture of the MDCK cells comprises the following steps: 1) preparing mixed liquid: dissolving and mixing the raw materials; and 2) regulating pH: regulating pH of the mixed liquid to 6.3-6.7, and setting a constant volume, so that the serum-free medium for full-suspension culture of the MDCK cells is obtained. The medium supports the high-density full-suspension culture of the MDCK single cells and is capable of greatly shortening a time of domesticating the MDCK cells from adherent cells into the serum-free full-suspension cells; and the medium is applicable to the large-scale production of biological products, in particular veterinary biological products.

The invention provides a composition useful to prepare high titer influenza viruses, e.g., in the absence of helper virus, which includes internal genes from an influenza virus vaccine strain or isolate, e.g., one that is safe in humans, for instance, one that does not result in significant disease, that confer enhanced growth in cells in culture, such as MDCK cells, or in eggs.