Canine distemper virus (CDV) sensitive cell line and establishment method and application thereof

A technology of canine distemper virus and sensitive cells, applied in the biological field, can solve the problems of decreased CDV virulence, unobvious virus cell lesions, and biological safety hazards
CN102807970AInactive Publication Date: 2012-12-05HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2012-12-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a canine distemper virus (CDV) sensitive cell line and an establishment method and application thereof, and belongs to the technical field of biology. According to the hundestaupe virus sensitive cell line, lentivirus four-plasmid packaging system is adopted to obtain a strain of stable cell line madin-darby canine kidney-cell isolate (MDCK-CSL) which expresses canine source signal lymphocyte activating molecules (SLAM), wherein the conservation number of strains is CGMCC No. 5881. The comparison of the propagation conditions of a CDV standard strain on the MDCK-CSL and MDCK cells indicates that after the CDV-Snyder Hill standard strain infects the MDCK-CSL cell line for 24 hours, cytopathy of the fusion, rounding contraction, obvious death and the like of cells can be observed; and the MDCK cells infected for 6 days continuously grow slowly, and the cytopathy does not occur. The CDV sensitive cell line MDCK-CSL provides a technical platform for the separation of CDV wild strains and the complete study of biological properties of the CDV wild strains, and also establishes a foundation for the prevention and control of canine distemper.
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Description

technical field

[0001] The invention relates to a sensitive cell line used for virus culture, in particular to a sensitive cell line used for canine distemper virus culture. The invention also relates to the establishment method and application of the cell line, belonging to the field of biotechnology. Background technique

[0002] Canine distemper (CD) is an acute, highly contagious infectious disease caused by canine distemper virus (CDV) infection in dogs. Since it was first reported in 1809, the disease has been distributed worldwide, causing great harm to the dog industry, fur animal breeding industry and wildlife protection industry. CDV belongs to the genus Morbillivirus of the family Paramyxoviridae. Although CDV is divided into six types: Asia-I, Asia-II, Europe, USA-II, Arctic and Vaccine Different genotypes, but only one serotype is recognized. CDV is not easy to survive in the environment, and the success rate of virus isolation on site is low, which limits th...

Claims

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