2 results about "Canine kidney" patented technology

Disclosed is an adapted Madin-Darby canine kidney cell line capable of suspension culture in the absence of serum, and a chemically-defined medium for culture of the adapted MDCK cell line. Further disclosed are culture methods for growing the adapted MDCK cell line and methods for producing a vaccine from the adapted MDCK cell line grown in the chemically-defined medium.

The invention discloses a canine kidney cell strain suitable for serum-free full suspension culture and an application of the canine kidney cell strain. The name of the canine kidney cell strain is MDCK-JX, the canine kidney cell strain is classified and named as MDCK canine kidney cells, and the preservation number of the canine kidney cell strain in the China General Microbiological Culture Collection Center is CGMCC No.46059. The canine parvovirus can be harvested after being cultured in full-suspension serum-free cultured MDCK-JX cells for 48 hours, the titer of the obtained virus liquid is greater than 107.5 TCID50 / mL, the titer of the virus is far higher than that of the virus cultured in a spinner bottle, the harvested canine parvovirus suspension virus can be used as a suspension seed virus for subculture, and the canine parvovirus suspension virus has the advantages of small inter-batch difference, good immune effect and high safety, and has a good application prospect. Meanwhile, the antigen production method is simple to operate, low in labor intensity and easy to realize large-scale culture, achieves the purposes of increasing the yield and reducing the cost, and has a good market prospect.