3 results about "Canine kidney" patented technology

The invention relates to an efficient screening method and application of an influenza virus candidate vaccine strain, and the efficient screening method comprises the following steps: (1) diluting a mother generation influenza virus to be screened by using a virus maintenance solution, adding the diluted mother generation influenza virus into a culture plate containing canine kidney cells, adsorbing the canine kidney cells by the influenza virus, and discarding a virus solution in the culture plate; (2) covering the surfaces of the cells with a maintenance medium in a liquid state, cooling and solidifying the maintenance medium, and inversely placing the maintenance medium in an incubator to culture viruses; and (3) selecting virus spots, mixing the selected virus spots with a buffer solution, inoculating the obtained mixture into chick embryos and / or canine kidney cells, and culturing to obtain the candidate vaccine strain of the influenza virus. The method provided by the invention is simple and easy to operate, short in time consumption, low in cost, few in passage times and not easy to mutate, and can achieve the purpose of quickly and effectively screening out influenza virus vaccine candidate strains with excellent growth characteristics from mother generation virus seeds in a short time.

Disclosed is an adapted Madin-Darby canine kidney cell line capable of suspension culture in the absence of serum, and a chemically-defined medium for culture of the adapted MDCK cell line. Further disclosed are culture methods for growing the adapted MDCK cell line and methods for producing a vaccine from the adapted MDCK cell line grown in the chemically-defined medium.

The invention discloses a canine kidney cell strain suitable for serum-free full suspension culture and an application of the canine kidney cell strain. The name of the canine kidney cell strain is MDCK-JX, the canine kidney cell strain is classified and named as MDCK canine kidney cells, and the preservation number of the canine kidney cell strain in the China General Microbiological Culture Collection Center is CGMCC No.46059. The canine parvovirus can be harvested after being cultured in full-suspension serum-free cultured MDCK-JX cells for 48 hours, the titer of the obtained virus liquid is greater than 107.5 TCID50 / mL, the titer of the virus is far higher than that of the virus cultured in a spinner bottle, the harvested canine parvovirus suspension virus can be used as a suspension seed virus for subculture, and the canine parvovirus suspension virus has the advantages of small inter-batch difference, good immune effect and high safety, and has a good application prospect. Meanwhile, the antigen production method is simple to operate, low in labor intensity and easy to realize large-scale culture, achieves the purposes of increasing the yield and reducing the cost, and has a good market prospect.