Culture medium for preparing influenza vaccine through MDCK cells and application method thereof

A technology of influenza vaccine and culture medium, which is applied in the field of culture medium for preparing influenza virus vaccine by cultivating MDCK cells, can solve the problems that the difference between product batches is difficult to control, the research needs to be further carried out, and the composition is difficult to be determined, so as to achieve good cell morphology and reduce risk, fast-growing effects
CN103074293AActive Publication Date: 2013-05-01杭州国牧生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
杭州国牧生物科技有限公司
Publication Date
2013-05-01

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Abstract

The invention provides a culture medium suitable for large-scale high-density cultivation of MDCK (Madin Darby Canine Kidney) cells for preparation of influenza vaccine.
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Description

technical field

[0001] The invention belongs to the field of biological products, and relates to a method for configuring and using a medium, and more specifically, relates to a medium for preparing influenza virus vaccines by culturing MDCK cells in a large-scale high-density growth environment. Background technique

[0002] The production of final products through animal cell culture is one of the most commonly used production methods in the field of biological products. Most of the medium used for animal cell culture needs to add 10%-15% animal serum to provide the nutrients needed for cell growth and the supporting proteins for cells to maintain normal shape. However, the disadvantages of animal-derived serum are: the composition is difficult to determine, the difference between product batches is not easy to control, the price is expensive, and extremely strict testing procedures are required to ensure that the serum does not contain bacteria, viruses, etc. that may be ...

Claims

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