Method for producing influenza virus vaccine

A production method and technology for influenza vaccine, which are applied in the fields of biochemical equipment and methods, antiviral agents, viruses/phages, etc., and can solve problems involving biosafety and public health, difficulty in harmless treatment, and production of avian influenza viruses, etc. , to achieve the effect of simple and stable production process, small footprint and high degree of automation

Active Publication Date: 2011-01-26
成都史纪生物制药有限公司
View PDF0 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easily polluted by the environment; the difference between different batches of chicken embryos is large; it is difficult to expand production; the chicken embryo itself is contaminated by bacteria or other viruses to produce vaccines There may be quality hazards; the number of waste embryos is large, and it is difficult to harmlessly dispose of them, which involves biosafety and public health issues
In addition, there have been reports on the use of bioreactor microcarriers to cultivate rabies vaccines, but there have been no reports on the use of bioreactor microcarriers to produce influenza vaccines for avian influenza viruses and other influenza viruses such as swine influenza, canine influenza, and equine influenza

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing influenza virus vaccine
  • Method for producing influenza virus vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A method for producing an avian influenza vaccine, comprising the steps of:

[0027] (1) Passaging and culturing of cells for seedling preparation: MDCK cells were taken, digested and passaged by EDTA-trypsin cell dispersion solution (Hank's solution containing 0.25% trypsin (1:250), 0.02% EDTA), and the cells containing 90% DMEM solution, 10% bovine serum, penicillin sodium and streptomycin sulfate at 200 units / ml each, the cell growth medium adjusted to pH 7.2 to continue culturing, the culturing temperature is 37°C, and when a good cell monolayer is formed, use Continue to subculture or inoculate in a bioreactor for microcarrier suspension culture, wherein the bioreactor is a bioreactor that can automatically control parameters such as temperature, pH, dissolved oxygen, stirring speed, etc., and is suitable for microcarrier suspension culture;

[0028] (2) Propagation of cytotoxic species: with virus culture fluid, the avian influenza (H9 subtype) seed virus is dilut...

Embodiment 2

[0035] A production method of swine flu vaccine, comprising the steps of:

[0036](1) Passage and culture of cells for seedling production: take MDCK cells and digest them with EDTA-trypsin cell dispersion (Hank's solution containing 0.25% trypsin (1:250) and 0.02% EDTA by mass volume fraction) Subculture, continue to culture with the cell growth medium containing 95% DMEM solution by weight fraction, 5% bovine serum, 250 units / ml of penicillin sodium and streptomycin sulfate, and adjust the pH to 7.3, and the culture temperature is 37°C to form good cells In the case of a single layer, it is used to continue subculture or inoculate in a bioreactor for microcarrier suspension culture. The bioreactor can automatically control parameters such as temperature, PH, dissolved oxygen, stirring speed, etc., and is suitable for the biological reaction of microcarrier suspension culture. device;

[0037] (2) Propagation of cytotoxic species: use virus culture medium to inoculate the go...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for producing a vaccine for avian influenza viruses and other influenza viruses such as a swine influenza virus, a canine influenza virus and an equine influenza virus. The method comprises the following steps of: subculturing cells for preparing the vaccine; (2) reproducing cytotoxic varieties; (3) performing microcarrier suspension culture on darby canine kidney (MDCK) cells in a bioreactor; (4) reproducing venom for preparing the vaccine; and (5) harvesting virus liquid. The method has the advantages of great reduction in production cost, short production period, no restriction to raw material supply, small occupied area, easy and quick expansion in production scale, low and readily treated environmental pollution, high degree of automation, few labors, easy equilibrium and stabilization in quality and obvious improvement on the yield and the quality of the vaccines.

Description

technical field [0001] The invention relates to a method for producing influenza vaccines of avian influenza virus and other influenza viruses such as swine influenza, canine influenza and equine influenza by using bioreactor microcarrier cell culture. It can be used for industrial production of influenza vaccines for avian influenza virus and other influenza viruses such as swine influenza, canine influenza, and equine influenza, replacing the traditional chicken embryo culture method. Background technique [0002] At present, the production of influenza vaccines for domestic avian influenza virus and other influenza viruses such as swine influenza, canine influenza, and equine influenza only relies on the chicken embryo method. The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easily polluted by the environment; the difference between different batches of chicken embryos is large; it is difficult to expand pro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00A61K39/145A61P31/16
Inventor 高鹏刘玉才胡来根王立斌严成徐志春
Owner 成都史纪生物制药有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap