Method for producing influenza virus vaccine

Abstract

The invention discloses a method for producing a vaccine for avian influenza viruses and other influenza viruses such as a swine influenza virus, a canine influenza virus and an equine influenza virus. The method comprises the following steps of: subculturing cells for preparing the vaccine; (2) reproducing cytotoxic varieties; (3) performing microcarrier suspension culture on darby canine kidney (MDCK) cells in a bioreactor; (4) reproducing venom for preparing the vaccine; and (5) harvesting virus liquid. The method has the advantages of great reduction in production cost, short production period, no restriction to raw material supply, small occupied area, easy and quick expansion in production scale, low and readily treated environmental pollution, high degree of automation, few labors, easy equilibrium and stabilization in quality and obvious improvement on the yield and the quality of the vaccines.

Description

technical field [0001] The invention relates to a method for producing influenza vaccines of avian influenza virus and other influenza viruses such as swine influenza, canine influenza and equine influenza by using bioreactor microcarrier cell culture. It can be used for industrial production of influenza vaccines for avian influenza virus and other influenza viruses such as swine influenza, canine influenza, and equine influenza, replacing the traditional chicken embryo culture method. Background technique [0002] At present, the production of influenza vaccines for domestic avian influenza virus and other influenza viruses such as swine influenza, canine influenza, and equine influenza only relies on the chicken embryo method. The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easily polluted by the environment; the difference between different batches of chicken embryos is large; it is difficult to expand pro...

AI Technical Summary

Problems solved by technology

The traditional process is labor-intensive, time-consuming, low in efficiency, and high in production cost; it is easily polluted by the environment; the difference between different batches of chicken embryos is large; it is difficult to expand production; the chicken embryo itself is contaminated by bacteria or other viruses to produce vaccines There may be quality hazards; the number of waste embryos is large, and it is difficult to harmlessly dispose of them, which involves biosafety and public health issues

In addition, there have been reports on the use of bioreactor microcarriers to cultivate rabies vaccines, but there have been no reports on the use of bioreactor microcarriers to produce influenza vaccines for avian influenza viruses and other influenza viruses such as swine influenza, canine influenza, and equine influenza

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