The invention relates to the technical field of microalgae
biology, in particular to a high-
biomass and high-photosynthetic-efficiency
chlorella pyrenoidosa
mutant strain J-1 which is obtained by mutagenizing
wild type chlorella pyrenoidosa through an
atmospheric pressure room temperature plasma (ARTP)
mutagenesis technology and combining an efficient screening strategy. When the strain is cultured for 4 days under a standard autotrophic condition, the
biomass reaches 1.23 mg / mL, the OD750 reaches 6.27, and when the strain is cultured for 5 days under a high
light stress condition, the OD750 reaches 16.70; according to the invention, the growth performance is obviously enhanced: the
biomass of J-1 is higher than that of WT under autotrophic, mixotrophic or heterotrophic conditions, and the biomass of a J-1
mutant strain reaches 1.23 mg / mL and is increased by 53.8% compared with that of a
wild type (0.80 mg / mL) after the J-1
mutant strain is cultured for 4 days under a standard autotrophic condition (50 [mu] mol * m <-2 > * s <-1 >); the OD750 reaches 6.27 and is higher than 5.75 of a
wild type, the OD750 reaches 2.18 mg / mL (WT is 1.75 mg / mL) on the fourth day of mixotrophic culture, the OD750 reaches 1.39 mg / mL (WT is 1.05 mg / mL) on the fourth day of heterotrophic culture, and it is indicated that biomass can be efficiently accumulated in different nutritional
modes.