Plants having improved characteristics and a method for making the same

A plant and plant seed technology, applied in the field of molecular biology, can solve problems not mentioned

Inactive Publication Date: 2009-10-21
CROPDESIGN NV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is not mentioned that those aspects of yield can be improved by inhibiting the expression of AtSIK

Method used

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  • Plants having improved characteristics and a method for making the same
  • Plants having improved characteristics and a method for making the same
  • Plants having improved characteristics and a method for making the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 40

[0802] The table of Example 40 gives examples of orthologs and paralogues of the SYB1 protein represented by SEQ ID NO:286. Using the BLAST process described above, other orthologs and paralogs can be easily identified.

[0803] Through three conserved ZnF_RBZ type zinc finger domain domains ( Figure 22 The appearance of (shown) can identify the protein of the present invention. The terms "domain" and "motif" or "tag" are defined in the "definitions" section. There are also specialized databases for identifying structural domains, such as SMART (Schultz et al. (1998) Proc. Natl. Acad. Sci. U.S. 95, 5857-5864; Letunic et al. (2002) Nucleic Acids Res 30, 242-244), InterPro ( Mulder et al. (2003) Nucl. Acids. Res. 31, 315-318), Prosite (Bucher and Bairoch (1994), generalized map syntax for biomolecular sequence motifs and its function in automated sequence interpretation, ( In) ISMB-94; Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology. ...

Embodiment 1

[0906] Example 1: Identification of sequences related to the nucleic acid sequence used in the method of the present invention

[0907] Use database sequence search tools, such as the Basic Local Alignment Tool (BLAST) (Altschul et al. (1990) J. Mol. Biol. 215: 403-410; and Altschul et al. (1997) Nucleic Acids Res. 25: 3389-3402) in Among those sequences maintained in the Entrez nucleotide database of the National Center for Biotechnology Information (NCBI), sequences (full-length cDNA, EST or genome) related to the nucleic acid sequence used in the method of the present invention were identified. This program is used to find regions of local similarity between sequences by comparing nucleic acid sequences or polypeptide sequences with sequence databases and calculating the statistical significance of matches. For example, the TBLASTN algorithm is used for the polypeptide encoded by the nucleic acid used in the present invention, and default settings and filtering are used to igno...

Embodiment 2

[0913] Example 2: Gene cloning AZ

[0914] The Arabidopsis AZ encoding gene (CDS3104) was amplified by PCR using an Arabidopsis seedling cDNA library (Invitrogen, Paisley, UK) as a template. After reverse transcription of RNA extracted from seedlings, cDNA was cloned into pCMV Sport 6.0. The average size of the inserts in the library is 1.5 kb and the initial number of clones is 1.59×10 7 cfu. In 6×10 11 After the first round of amplification of cfu / ml, the original titer was determined to be 9.6×10 5 cfu / ml. After plasmid extraction, 200 ng template was used in 50 μl PCR mix. The primer prm06717 (sense, the AttB1 site is in italics, and the start codon is in bold: 5’- tqctqtqqatcaqacc-3') (SEQID NO 7) and prm06718 (antisense, complementary: the position is in italics: 5'- ggttag gtctctca attctgc·-3') (SEQ ID NO 8) is used for PCR amplification, wherein the primer includes an AttB site for Gateway recombination. PCR was performed using Hifi Taq DNA polymerase under standard cond...

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PUM

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Abstract

The present invention relates generally to the field of molecular biology and concerns a method for enhancing various economically important characteristics in plants. More specifically, the present invention concerns a method for improving yield-related traits, such as enhanced yield and / or enhanced growth, or modified content of storage compounds in plants by modulating expression in a plant of a nucleic acid encoding a GRP (Growht Related Protein) polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a GRP polypeptide, which plants have improved characterisitics relative to control plants. The invention also provides hitherto unknown GRP-encoding nucleic acids, and constructs comprising the same, useful in performing the methods of the invention.

Description

[0001] The present invention generally relates to the field of molecular biology and relates to methods for improving various plant characteristics by modulating the expression of a nucleic acid encoding GRP (growth-related protein) in plants. The present invention also relates to plants having regulated expression of a nucleic acid encoding a GRP polypeptide, wherein the plants have improved characteristics relative to corresponding wild-type plants or other control plants. The invention also provides constructs for use in the methods of the invention. [0002] The growing world population and the shrinking supply of arable land for agriculture have stimulated research on increasing agricultural efficiency. Conventional crop and horticultural improvement methods use selective breeding techniques to identify plants with popular traits. However, such selective breeding techniques have several drawbacks, namely, these techniques generally consume a lot of labor and produce plants that...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H1/00
CPCC12N15/8271
Inventor V·弗兰卡德V·米隆诺弗C·勒佐A·I·桑兹莫林纳罗
Owner CROPDESIGN NV
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