36 results about "Egg cell" patented technology

The invention relates to a formula of a tea drink, and relates to the technical field of homology of medicine and food, and the formula is prepared by compounding nine components of double-petal red rose, Hetian jade jujube slices, dried mulberry, longan pulp, Ningxia wolfberry, Xinhui dried orange peel, raspberry, Hunan lotus seed pulp and licorice slices according to a specific proportion. Synergistic interaction is realized through triple action mechanisms, the liver soothing and depression relieving of the double-petal red rose is taken as a regulation and control core, the cortisol level is reduced, and endocrine imbalance is improved; on the basis of benefiting qi and nourishing blood by jujube slices and Chinese wolfberry fruits, dried mulberries and raspberries are enriched with natural folic acid and antioxidant substances, so that the egg cell quality and reproductive microcirculation are improved in a targeted manner; longan-Hunan lotus seed meat is used for warming uterus and tonifying spleen, and dried orange peel-licorice tablets are combined to enhance component absorption and compatibility stability. The preparation method comprises the steps of gradient drying, precise mixing and cobalt-60 irradiation sterilization. According to the scheme, the absorption bottleneck of traditional substitutional tea is broken through, the bioavailability is improved, and the compound sub-health problems of female endocrine disorder, follicular development disorder and cold uterus and weak body are quantitatively solved.

The first objective is to identify previously unknown factors or combinations of factors that can efficiently produce oocyte-like cells from pluripotent stem cells, etc. The second objective is to provide oocyte-like cells and an efficient method for producing them. [Solution] A method for producing egg-like cells, which includes a step of introducing a factor into a cell, wherein the factor includes DLX6 or a nucleic acid encoding DLX6, and egg-like cells derived from human pluripotent stem cells and expressing PADI6.

Methods for improving parthenogenesis efficiency by DWT1 and BABY BOOM transcription factors in plants are provided. A rice embryo trigger transcription factor BABY BOOM1 can initiate embryogenesis when expressed in the unfertilized egg cell through a process called parthenogenesis (Khanday et al., 2019. Nature 565: 91-95). The parthenogenesis efficiency by BABY BBOM1 itself is 10-29%. This invention describes methods of high frequency of parthenogenesis by simultaneous expression of BABY BOOM and DWT1 transcription factors. When BABY BOOM1 and DWT1 are expressed together through egg cell-specific promoters, parthenogenesis efficiencies of up to 90% are achieved. These high parthenogenesis efficiencies are a prerequisite for field applications of synthetic apomixis in crop plants.

This invention discloses a two-branch oocyte target detection method and system for motion blur. Addressing the motion blur problem caused by equipment vibration and oocyte micro-movement during oocyte retrieval, this invention constructs an end-to-end detection model comprising a clear branch and a blurred branch. Each branch includes a lightweight feature recovery network, a multi-band feature fusion module, a frequency structure enhancement module, and an oocyte target detection network. During the training phase, feature domain deblurring loss, feature consistency self-supervised loss, and oocyte detection loss are used for joint training of the two branches, with the clear branch's features supervising the learning of robust feature representations in the blurred branch. During the inference phase, only the blurred branch outputs the detection results. The multi-band feature fusion module repairs degraded features such as contours, edges, and textures through frequency domain decomposition and targeted enhancement, while the frequency structure enhancement module compensates for missing structural information through a cross-attention mechanism. This invention effectively solves the problem of decreased detection accuracy caused by motion blur.

The invention discloses a method for constructing a mammalian leucopenia model based on a Crispr gene editing technology. The invention belongs to the technical field of biology, and particularly relates to a method for constructing a mammalian leucopenia model based on a Crispr gene editing technology. The construction method of the leucopenia mouse model comprises the following steps: specifically mutating an H4K31 site in a mouse fertilized egg cell, so that the site is changed into arginine from lysine, and the leucopenia mouse model is obtained. A corresponding gene sequence is mutated at an H4K31 site in a fertilized egg cell of the specifically mutated mouse by means of cutting of a Crispr gene editing system and a fixed-point knock-in technology of homologous recombination. By detecting the immune function regulation and control mechanism of a homozygous mouse with the H4K31 site after the homozygous mouse with the H4K31 site is prepared, the mouse shows a natural leucopenia phenotype after lysine at the H4K31 site is mutated into arginine.

The present application relates to a kind of CD69 humanization mouse model construction method, the construction method: (1) the targeting vector of expression humanization CD69 gene is constructed;(2) design and obtain sgRNA for mouse Cd69 gene;(3) the targeting vector, sgRNA and Cas9 protein are co-injected or co-electrotransferred to mouse fertilized egg cytoplasm or nucleus, and the fertilized egg is transplanted to pseudopregnant mouse, and genotype identification is carried out to pseudopregnant litter mouse, and the positive F0 mouse of correct human source fragment successfully inserted is screened;(4)F0 mouse is mated with background mouse to obtain F1 mouse, and CD69 humanization mouse model is screened out.The CD69 humanization mouse constructed in the present application has application value in immunology and other fields.

The invention discloses a litopenaeus vannamei egg membrane outer layer protein VMO1 and an application thereof in egg entering of a prawn foreign protein. The amino acid sequence of the egg membrane outer layer protein VMO1 of the litopenaeus vannamei is as shown in SEQ ID NO. 2. The litopenaeus vannamei vitelline membrane outer layer protein gene LvVMO1 is cloned from litopenaeus vannamei for the first time, and experiments prove that the litopenaeus vannamei vitelline membrane outer layer protein gene LvVMO1 is generated in the hepatopancreas tissue of the litopenaeus vannamei and enters eggs through a hemolymph way. A VMO1-EGFP in-vivo tracing test further shows that VMO1 is generated in hepatopancreas of the litopenaeus vannamei, enters the ovary through hemolymph, can carry foreign proteins such as EGFP, is transferred from an abdominal injection site and is enriched in the ovary. The invention provides an effective technical means for introducing a gene editing system into litopenaeus vannamei egg cells by using foreign proteins to realize genetic improvement and other applications of litopenaeus vannamei, and has wide application prospects and great economic values.

Provided is a method for constructing a humanized FKBP8 gene knock-in animal model, comprising: (1) constructing a targeting vector for humanized FKBP8 gene knock-in, and transfecting the targeting vector, Cas9, and sgRNA into fertilized eggs of donor animals; (2) transplanting surviving fertilized egg cells into the oviduct of pseudopregnant animals to obtain F0 generation animals and identifying the F0 generation animals, wherein animals showing positive homologous recombination are chimeric animals; and (3) mating the chimeric animals with wild-type animals to obtain F1 generation animals and performing identification, wherein F1 animals showing positive homologous recombination are humanized FKBP8 knock-in animals. In the construction method, an FKBP8 gene sequence in a donor animal is substituted by a human FKBP8 gene sequence, so as to achieve the purpose of humanizing the FKBP8 gene in the donor animal, thus successfully establishing a humanized FKBP8 gene knock-in animal model, so that screening for drugs targeting the FKBP8 gene and a product thereof in the animal model, or studying the pathogenesis and treatment mechanisms of the FKBP8 gene and the product thereof in related clinical diseases can be realized.

During aging, egg cells exhibit gradual loss of adherin complexes. The absence of connexin ultimately results in premature separation of sister staining monomers, resulting in aneuploidy. The inventors have designed an artificial adhesion system that binds staining monomers or chromosomes by forming complexes, thereby reducing the phenomena of premature separation of age-related sister staining monomers, e.g., in egg cells. The artificial adhesion system is a complex, and the aneuploidy risk is reduced by binding chromosomes or dyeing monomers. The complex comprises (I) one or more first proteins and (II) one or more second proteins wherein: (I) the first protein and (II) the second protein each comprise (i) a chromatin binding component, (ii) a protein binding region located at the N-terminus of the chromatin binding component, (Hi) a protein binding region located at the C-terminus of the chromatin binding component. The invention also includes nucleic acid molecules encoding the complexes. In addition, several in vitro methods associated with the complexes or nucleic acid molecules encoding said complexes also constitute part of the invention.

The invention relates to a mitochondrial delivery system and a preparation method and application thereof. According to the mitochondrial delivery system, safe and efficient cell entry of active mitochondria can be achieved, the mitochondrial delivery system has appropriate particle size and surface potential, toxicity to cells is avoided while the entrapment rate of the mitochondria is effectively improved and the electrochemical stability and biological activity of a mitochondrial membrane are guaranteed, and the mitochondrial cell entry efficiency is remarkably improved. The mitochondrial delivery system not only can transplant active mitochondria into common cells to repair cell damage, but also can transplant the active mitochondria into egg cells to play a role across a zona pellucida and a plasma membrane barrier. The mitochondrial delivery system can be used for treating mitochondrial diseases related to mitochondrial dysfunction, especially infertility caused by mitochondrial dysfunction of egg cells.

This invention relates to the field of cell biology, and more particularly to a method for constructing an artificial cell model in vitro based on extracts from Xenopus laevis egg cells. The method includes: mixing cytoplasmic extracts from Xenopus laevis egg cells, demembranous sperm, and tubulin, dispersing the mixture in an oil phase to obtain a reconstructed system; reacting the reconstructed system at 20-24°C for at least 30 minutes, resulting in the formation of a phospholipid bilayer-based cytoplasmic membrane structure around the cell. This invention provides a method for constructing artificial cells by selecting cytoplasmic extracts from Xenopus laevis egg cells, adding specific substances, and reacting under specific conditions to allow the extracts to self-assemble into cell structures in vitro, simultaneously forming a cytoplasmic membrane. The method provided by this invention offers important clues to the origin and evolution of cells, and the resulting artificial cell structures are highly similar to normal cells, possessing significant application value.

The invention relates to the technical field of fish breeding. The invention provides an artificial breeding method for paraphyllous hemsleyanus, which comprises the following steps: (1) selecting parent paraphyllous hemsleyanus which is healthy, free of injury and disease, good in gonad development and more than 18 months old, breeding male and female parent fishes in different ponds, and controlling the ratio of male fishes to female fishes to be 1: (2-3); (2) selectively injecting a first oxytocic into the mature male fish, and injecting a second oxytocic into the sexually mature female fish to obtain induced spawning parent fish; (3) collecting seminal fluid of the induced spawning male fish and fish egg cells of the induced spawning female fish, carrying out artificial insemination, and hatching to obtain fry. By adopting the breeding method, generation of parent fish egg cells or sperms can be effectively promoted, so that the quality of fertilized eggs and the breeding success rate are improved; by utilizing the compound spawning induction substance, the spawning induction effect is enhanced, meanwhile, the use of chemical substances can be reduced, and drug residues and environmental pollution are reduced. The method is of great significance to artificial breeding and large-scale breeding of the paralifola speculatus.

This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters.

The invention discloses a method for rapidly establishing a respiratory syncytial virus susceptible animal model based on adenovirus transduction and application, and belongs to the technical field of animal models. The method comprises the following steps: constructing a homologous arm targeting vector carrying human-derived hIGF1R; the method comprises the following steps: injecting a homologous arm targeting vector, specific sgRNA and mRNA encoding Cas9 enzyme into a mouse fertilized egg cell to obtain an F0-generation positive mouse, and mating the F0-generation positive mouse with a wild-type same-strain mouse to obtain an F1-generation heterozygote mouse; the respiratory syncytial virus susceptible model mouse precursor is obtained. Cre enzyme can be transferred through an adenovirus transduction technology, and an STOP element between the promoter and an hIGF1R molecule is cut off, so that human hIGF1R receptor gene expression is quickly started. The animal model not only can reproduce a virus invasion path in a human body, but also can be used as a young small animal model for strict vaccine effectiveness and safety tests, has the characteristics of rapidness and high efficiency compared with a traditional method, and is suitable for in-vivo effectiveness evaluation and infection mechanism research of vaccines, antibodies and drugs under emergency conditions.

During ageing, egg cells display a gradual loss of cohesin complexes. The cohesin loss eventually causes sister chromatids to separate prematurely, which leads to aneuploidy. The inventors engineered an artificial cohesion system, which is a complex for chromatid or chromosome tethering to reduce, for example, age-related premature separation of sister chromatids in eggs. The artificial cohesion system is a complex, which tethers chromosomes or chromatids so that e.g. the risk of aneuploidy is reduced. Said complex comprises (I) one or more first protein(s) and (II) one or more second protein(s), wherein the (I) first and the (II) second protein(s) each comprise (i) a chromatin-binding component, (ii) a protein-binding region being N-terminal of the chromatin-binding component, (Hi) a protein-binding region being C-terminal of the chromatin-binding component. The present invention also includes nucleic acid molecule(s) encoding said complex. Furthermore, several in vitro methods concerning the complex or the nucleic acid molecule(s) encoding said complex also form part of the invention.

A composition includes a contraceptive chimeric virus-like particle with an antigenic carrier domain and one or more antigenic regions from a sperm cell in the antigenic carrier domain, with the antigenic carrier domain including human papillomavirus L1 capsid protein and the antigenic regions including one or more structural elements of the Catsper ion channel complex. When administered to a patient, the contraceptive vaccine stimulates production of anti-sperm antibodies that, upon binding to a sperm cell, inhibit the sperm cell's motility and thus inhibit the ability of the sperm cell to fertilize an egg cell. The induced immunoinfertility of the composition can be reversed for brief or extended lengths of time by overdosing the patient with a reversal agent lacking the antigenic carrier domain but having a protein sequence substantially identical to that of the one or more antigenic regions to sequester the anti-sperm antibodies.

The present application relates to the field of breeding and cultivation of pearl mussels, and particularly relates to a genetic transformation method for freshwater pearl mussels based on Agrobacterium mediation, comprising the following steps: soaking the female pearl mussels in the oogenesis or fertilization stage in Agrobacterium transformation liquid, and screening and culturing the transgenic freshwater pearl mussel offspring. The reproductive cell mass with successful genetic transformation is detected, the introduction and integration of the exogenous gene in the reproductive cells of the pearl mussel are successfully realized, the blank of the genetic transformation tool and technology for mollusks is filled, and the key guidance for the molecular breeding practice of the pearl mussel is provided.

The present invention relates to a method for constructing a TFRC humanized mouse model, comprising: (1) constructing a targeting vector expressing a humanized TFRC gene; (2) designing and obtaining a sgRNA targeting the mouse TFRC gene; (3) co-injecting or co-electroporating the targeting vector, sgRNA, and Cas9 protein into the cytoplasm or nucleus of a mouse fertilized egg, transplanting the fertilized egg into a pseudopregnant mouse, performing genotyping on the pseudopregnant offspring, and screening for positive F0 mice in which the correct humanized fragment was successfully inserted; and (4) breeding the F0 mice with background mice to obtain F1 mice, and screening for a TFRC humanized mouse model. The TFRC humanized mouse constructed by the present invention has application value in fields such as oncology and immunology.

The utility model relates to the technical field of low-temperature storage of cow egg cells, and discloses a low-temperature storage device for cow egg cells, which comprises a fixed frame, supporting legs are fixedly connected to the bottom surface of the fixed frame, a storage mechanism is arranged in the fixed frame, and a dehydration mechanism is arranged on the top surface of the storage mechanism; the storage mechanism comprises a circulating assembly and a placing assembly, the circulating assembly is fixedly connected in the fixing frame, and the placing assembly is fixedly connected to the top surface of the circulating assembly; the placement assembly comprises a placement box, the placement box is arranged on the left side of the liquid nitrogen box, a second water pump is fixedly connected to the surface of the placement box, a first conveying pipe is fixedly connected to the output end of the second water pump, a molecular sieve is fixedly connected into the liquid nitrogen box, and a third water pump is fixedly connected to the surface of the liquid nitrogen box; a one-way air valve is fixedly connected to the top face of the containing box. The low-temperature storage device is provided with the storage mechanism, so that liquid nitrogen is reused, the cost is saved, and damage to egg cells is weakened by arranging the dehydration mechanism.

The invention relates to the technical field of biological medicine, in particular to a primer combination for rapidly and simply screening idiopathic male infertility population based on a CATSPER2 gene high-frequency mutation region and application of the primer combination. By means of real-time fluorescent quantitative PCR or common PCR and agarose electrophoresis combined analysis, products with corresponding large and small bands are amplified by using DNA and mRNA specific primers of CATSPER2, and the CATSPER2 gene high-frequency mutation region-based primer combination is obtained. And detecting whether the CATSPER2 gene has mutation or not by comparing the contents of products so as to evaluate whether the function of the CatSperer channel is abnormal or not and carry out precise treatment of ICSI (Intrasperm Sperm Injection) on such patients. According to the primer and the screening scheme provided by the invention, sterile sperms and normal sperms of the CATSPER2 gene caused by chromosome variation, large fragment lack or mutation can be clearly and effectively distinguished; according to the method, the sterile population caused by the abnormality of the CatSper channel can be quickly, simply and conveniently screened out, so that precise treatment of single sperm injection in egg cells of the population can be achieved, the treatment cycle is shortened, and the economic and psychological burden of patients is relieved.

The application discloses a culture solution containing lupeol for improving in-vitro maturation efficiency of pig oocytes and application thereof. The application adds lupeol, especially 4 muM lupeol, to an in-vitro maturation culture solution of oocytes, which can effectively improve the oxidation-reduction state of oocytes, significantly reduce the intracellular ROS level, and meanwhile, improve the GSH content, thereby maximally relieving oxidative stress damage in the in-vitro maturation process, significantly improving the maturation rate of pig oocytes in the meiotic metaphase II, and effectively reducing the DNA fragmentation ratio, which indicates that the lupeol has excellent effects of promoting nuclear maturation and protecting genetic material. The lupeol is added to the in-vitro maturation culture solution of oocytes, which is easy to operate and can be applied to the existing culture process, and can stably improve the efficiency and quality of pig in-vitro embryo production, and has important application value for pig germplasm resource preservation, rapid propagation of excellent breeds and development of animal husbandry.

This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters.

The invention discloses a test-tube baby fertilized egg cell auxiliary culture device which structurally comprises a storage box, a heating device and an operation panel, a sealing door is hinged to the upper portion of the storage box, a sealing rubber strip is arranged at the joint of the sealing door and the storage box, the whole storage box is made of heat preservation materials, and the heating device is arranged in the storage box. The device further comprises a culture tank, a driving device and a transmission device. The culture tank is rotationally arranged in the storage box. Supporting rods are fixedly arranged on the upper portion and the lower portion of the inner side of the storage box, and the driving equipment is arranged on the supporting rods. The driving equipment is movably connected with the culture tank, and the transmission equipment is arranged at the joint of the driving equipment and the culture tank. The invention belongs to the field of cell culture devices, and particularly relates to a test tube baby fertilized egg cell auxiliary culture device.

The invention discloses an allogenic material introduction method suitable for large-scale oyster egg cells, and belongs to the technical field of marine organisms. Comprising the following steps: (1) dissecting oysters to obtain egg cells, then mixing nucleic acid molecules to be delivered with a transfection reagent to prepare a transfection system, and mixing protein molecules capable of self-assembling to form a condensation body with the nucleic acid molecules in a buffer solution to form a protein-nucleic acid condensation body compound, the transfection reagent contains protein molecules which can be self-assembled to form a condensation body; and (2) mixing seawater containing egg cells with the protein-nucleic acid aggregate compound, incubating, and terminating the reaction. The method is convenient to operate, large in transfection flux and small in damage to cell morphology. The method is suitable for transfection systems of various sizes, and by taking a six-hole cell culture plate as an example, about 8 * 10 < 4 > egg cells can be simultaneously transfected in one hole at most under the condition that the final culture system is about 2ml.

The utility model belongs to the technical field of medical equipment, and particularly relates to an infertility ovulation collecting and storing device for reproductive medicine, which comprises a liquid nitrogen tank, a lower shell and an upper shell, the liquid nitrogen tank is arranged in the lower shell, one end of the upper shell is rotatably connected with the lower shell through a rotating shaft, the inner bottom of the lower shell is connected with a supporting plate through a plurality of damping shock absorbers, and the supporting plate is connected with the lower shell. The inner wall of the lower shell is slidably connected with two symmetrically-arranged arc-shaped clamping plates through a plurality of sliding rods, the sliding rods are sleeved with first springs, the two ends of the first springs are fixedly connected with the arc-shaped clamping plates and the inner wall of the lower shell correspondingly, the end, away from the rotating shaft, of the upper shell is slidably connected with a clamping mechanism, and the lower shell is provided with clamping grooves corresponding to the clamping mechanism. And the inner top of the upper shell is fixedly connected with a sponge pad. According to the egg cell collecting and storing device, due to the arrangement of a plurality of buffering and damping structures, a damping protection effect can be effectively achieved on the egg cell collecting and storing device, and therefore the use safety of the storing device is effectively improved.

Provided herein are methods and compositions for the detection of silent carriers of chromosomal deletion alleles in a human subject using haploid cells (e.g., sperm cells or egg cells) derived from the subject. The methods provided herein allow for the detection of silent (2+0) carriers of SMA, where the individual has a deletion of the SMN1 gene on one chromosome 5 homolog and two or more copies of the SMN1 gene on the other chromosome 5 homolog.

The invention provides a treatment method for increasing the hatching rate of fairy shrimp eggs, which comprises the following steps of: S1, hydrating the fairy shrimp eggs with sterile artificial seawater, and performing pulsed electric field sequence stimulation treatment by adopting a high-voltage pulsed electric field; s2, placing the fairy shrimp eggs in a constant-temperature and constant-humidity seawater water bath box for hatching so as to improve the hatching rate of the fairy shrimp eggs within 24 hours. According to the method, the high-voltage pulsed electric field technology is adopted for treating fairy shrimp eggs, a large number of through holes are formed in egg shells of the eggs, the shell breaking resistance of larvae is reduced, meanwhile, the activity of superoxide dismutase (SOD) in egg cells and the content of ROS free radicals in the cells are improved, and the metabolic development speed of egg embryos is increased; the hatching efficiency of the eggs is finally and efficiently improved through the double-effect mechanism of'inside-to-outside ', the defects of low efficiency, complex operation, drug residues and the like of the existing hatching technology of fairy shrimp hatching are overcome, meanwhile, the high-voltage pulse electric field device is very low in energy consumption and simple and convenient to operate, and the production cost can be effectively reduced.

The present invention belongs to the field of egg cell operation instruments, and discloses a sample precision controller and a using method thereof. Among them, the sample precision controller includes a main machine chamber, a handheld part and a connecting pipe. The main machine chamber is equipped with a display and a driving mechanism. The driving mechanism is provided with a connection port. The handheld part includes a gripping part, and a through connection channel is arranged on the gripping part. One end of the connecting pipe is communicated with the end of the connection channel, and the other end of the connecting pipe is communicated with the connection port. The driving mechanism is separated from the handheld part, which improves the control precision during the egg stripping operation.