8 results about "Egg cell" patented technology

This invention discloses a two-branch oocyte target detection method and system for motion blur. Addressing the motion blur problem caused by equipment vibration and oocyte micro-movement during oocyte retrieval, this invention constructs an end-to-end detection model comprising a clear branch and a blurred branch. Each branch includes a lightweight feature recovery network, a multi-band feature fusion module, a frequency structure enhancement module, and an oocyte target detection network. During the training phase, feature domain deblurring loss, feature consistency self-supervised loss, and oocyte detection loss are used for joint training of the two branches, with the clear branch's features supervising the learning of robust feature representations in the blurred branch. During the inference phase, only the blurred branch outputs the detection results. The multi-band feature fusion module repairs degraded features such as contours, edges, and textures through frequency domain decomposition and targeted enhancement, while the frequency structure enhancement module compensates for missing structural information through a cross-attention mechanism. This invention effectively solves the problem of decreased detection accuracy caused by motion blur.

This invention relates to the field of cell biology, and more particularly to a method for constructing an artificial cell model in vitro based on extracts from Xenopus laevis egg cells. The method includes: mixing cytoplasmic extracts from Xenopus laevis egg cells, demembranous sperm, and tubulin, dispersing the mixture in an oil phase to obtain a reconstructed system; reacting the reconstructed system at 20-24°C for at least 30 minutes, resulting in the formation of a phospholipid bilayer-based cytoplasmic membrane structure around the cell. This invention provides a method for constructing artificial cells by selecting cytoplasmic extracts from Xenopus laevis egg cells, adding specific substances, and reacting under specific conditions to allow the extracts to self-assemble into cell structures in vitro, simultaneously forming a cytoplasmic membrane. The method provided by this invention offers important clues to the origin and evolution of cells, and the resulting artificial cell structures are highly similar to normal cells, possessing significant application value.

A composition includes a contraceptive chimeric virus-like particle with an antigenic carrier domain and one or more antigenic regions from a sperm cell in the antigenic carrier domain, with the antigenic carrier domain including human papillomavirus L1 capsid protein and the antigenic regions including one or more structural elements of the Catsper ion channel complex. When administered to a patient, the contraceptive vaccine stimulates production of anti-sperm antibodies that, upon binding to a sperm cell, inhibit the sperm cell's motility and thus inhibit the ability of the sperm cell to fertilize an egg cell. The induced immunoinfertility of the composition can be reversed for brief or extended lengths of time by overdosing the patient with a reversal agent lacking the antigenic carrier domain but having a protein sequence substantially identical to that of the one or more antigenic regions to sequester the anti-sperm antibodies.

The application discloses a culture solution containing lupeol for improving in-vitro maturation efficiency of pig oocytes and application thereof. The application adds lupeol, especially 4 muM lupeol, to an in-vitro maturation culture solution of oocytes, which can effectively improve the oxidation-reduction state of oocytes, significantly reduce the intracellular ROS level, and meanwhile, improve the GSH content, thereby maximally relieving oxidative stress damage in the in-vitro maturation process, significantly improving the maturation rate of pig oocytes in the meiotic metaphase II, and effectively reducing the DNA fragmentation ratio, which indicates that the lupeol has excellent effects of promoting nuclear maturation and protecting genetic material. The lupeol is added to the in-vitro maturation culture solution of oocytes, which is easy to operate and can be applied to the existing culture process, and can stably improve the efficiency and quality of pig in-vitro embryo production, and has important application value for pig germplasm resource preservation, rapid propagation of excellent breeds and development of animal husbandry.

The present invention belongs to the fields of biotechnology and plant breeding. Specifically disclosed are a gene having parthenogenesis-inducing ability in plants, and the use thereof in plant breeding such as induction of haploid formation and apomixis. By means of regulating the expression of a gene that encodes a protein having parthenogenesis-inducing ability, the transition of egg cells into the embryonic development fate is effectively realized, thereby inducing parthenogenesis. The gene and a corresponding induction method therefor can be applied to related plant breeding processes such as the production of haploids and synthetic apomixis.

The application discloses a method and device for characterizing the viscoelasticity of an egg cell based on a nonlinear Maxwell model, a medium and a product, and relates to the field of the viscoelasticity of an egg cell. The method acquires a force-time curve sample set of a shellfish egg cell; a Maxwell model with a nonlinear index parameter is constructed; according to the force-time curve sample set, a differential evolution global optimization algorithm is used to optimize a unified parameter in the Maxwell model, with the maximum minimum determination coefficient in all samples as an optimization target, so that the optimized unified parameter is obtained; the Maxwell model with the optimized unified parameter is fitted and verified, so that it is determined that all samples reach a fitting standard, and a verified Maxwell model is obtained; and the verified Maxwell model is used to describe the nonlinear viscoelastic behavior of the shellfish egg cell. The application can accurately and universally describe the viscoelastic mechanical behavior of the shellfish egg cell.