191 results about "Egg cell" patented technology

An object of the present invention is to efficiently establish cells, tissues, and organs capable of serving as donors for treating diseases, without eliciting immune rejection reactions, without starting with an egg cell. This object was achieved by providing a pluripotent stem cell having a desired genome. The cell was produced by treating with a reprogramming agent, producing a fusion cell of an MHC deficient stem cell with a somatic cell, or after producing a fusion cell of a stem cell with a somatic cell, removing a gene derived from the stem cell by performing genetic manipulation with a retrovirus.

The invention relates to a paraffin wax slicing method of ocean shellfish oocytes, comprising the steps of fixing, dehydration, clarity, waxdip, imbedment, slicing, dyeing, mounting, and the like. Theparaffin wax slicing method is characterized by solving the problem of the difficult waxdip and imbedment of the ocean shellfish oocytes due to small volume, improving a hematoxylin-eosin dyeing method and obtaining favorable slicing and dyeing effect. The paraffin wax slicing method is simple and easy to operate without adding instruments or equipment, can carry out paraffin wax slicing on mostof the ocean shellfish oocytes with the diameters more than or equal to 40 micrometers, transition steps are carried out among the dehydration, the clarity and the waxdip of the steps and mostly in two-step processing so that a reagent gradually and completely enters a tissue and is preserved in the original structure of the tissue in an intact way so as to provide an effective researching means for shellfish fertilization and early embryonic development research.

The invention describes methods and agents for improving cosmetic appearance, for promoting, improving or restoring health of cells and tissues, preferably skin, and more preferably, for restoring aged or damaged skin to a healthy appearance. This invention relates to the use of cells and cellular extracts in rejuvenation and healing technologies thereby improving healing and regeneration of all bodily tissues and organs. The present invention relates to compositions and methods of managing, preventing, and treating scars. The invention also relates to prevention of deterioration, damage and malfunction of cells and tissues, and to promote, improve or exceed cellular function in order to promote, improve and exceed appearance, vitality and health. In some embodiments, the invention relates to compositions of cells, eggs, cell extracts, egg extracts, and extract components such as purified nucleic acids, polypeptides, lipids, carbohydrates or other natural products. Said components can also be synthesized. In other embodiments, the cells are differentiable cells preferably stem cells or eggs. In some embodiments, compositions are used in a method that comprises application of compositions to tissues, including skin and / or wounds after removal of tissue surface layers. In other embodiments, the invention relates to methods of dedifferentiation of cells and / or dedifferentiation followed by differentiation. In other embodiments, the invention relates to managing, preventing, and treating skin diseases.

The invention provides a composition useful to prepare high titer influenza viruses, e.g., in the absence of helper virus, which includes internal genes from an influenza virus vaccine strain or isolate, e.g., one that is safe in humans, for instance, one that does not result in significant disease, that confer enhanced growth in cells in culture, such as MDCK cells, or in eggs.

An egg carton comprises a base having a plurality of egg cells, the base being attached to a lid and locking flap by at least one hinge member. The lid has a plurality of closing apertures adapted to receive a plurality of buttons located on the locking flap. The lid further has an outer closure mechanism adapted to interlock with an inner closure mechanism located on the locking flap. The egg carton may also be equipped with a venting system.

This invention relates to a method for increasing the conversion efficiency of pollen tube pathway method by using agrobacterium tumefaciens virulence gene. The method comprises: combining or mixing agrobacterium tumefaciens virulence gene and the target gene to be converted (green fluorescent protein gene) in vitro, packaging with liposome, and converting plants, especially cotton by using pollen tube pathway method. The method combines agrobacterium-liposome-pollen tube pathway technique, and has obviously increased protoplast conversion efficiency. Plasmids are not only protected by liposome when entering embryo sacs, but also encapsulated by the toxic protein to be expressed when entering egg cells, which can improve the stability of exogenous DAN in cell conversion process, realize precise cutting in T-DNA region, and increase the conversion efficiency.

The invention provides an automatic device for egg cell in-vitro fertilization and cleavage culture. The device comprises a combined incubator, which comprises a plurality of divided areas, wherein the areas can be automatically communicated, and also comprises a central controller arranged outside the incubator, and an egg cell automatic recognizing and sorting device. The different areas in the incubator are provided with different operation modes in different phases. Each area is provided with an automatic gas-tight door, and thus the automatic communication / disconnection of each area can be achieved by the gas-tight door. The incubator is provided with a culture medium for bearing the egg cell drops and a culture medium driving device for driving the culture medium to move in the divided areas. The egg cell automatic recognizing and sorting device can recognize and sort the follicle liquid containing egg cells according to the received control commands and then transports the egg cell drops to the culture medium in the incubator. When the automatic gas-tight doors open according to the control commands, the culture medium driving device drives the culture medium to move around in the divided areas according to the control commands, and corresponding operations are performed on the egg cell drops when the culture medium enters a divided area.

The present invention is directed to a vector and its use to generate genetically modified animals and cells. One aspect of this invention involves a vector that comprises a sperm cell and one or more polynucleotide molecules bound to a sperm cell through one or more non-liposome based linkers. In a preferred embodiment of this invention, the linker is a protein or polypeptide, preferably sperm specific such as an antibody that binds with the external surface of the sperm cell. In another aspect of the present invention, genetically modified cells or animals are derived from the fertilization of an animal egg cell with the vector described. In one preferred embodiment, genetic modification occurs with the polynucleotide molecule integrating, wholly or partially, into the cell or animal's genome. Another aspect of the present invention includes cells, such as sperm cells or egg cells, and cell lines that are derived from these genetically modified animals or their descendants. In another aspect of the present invention, the genetically modified animals derived from the use of the sperm vector described above possess certain desired characteristics. Examples of these characteristics include faster growth rates, disease or pathogen resistance, high production of certain proteins in milk, and organs suitable for animal to human xenotransplantation.

An artificial reproduction method for lateen mussel or butterfly mussel includes such steps as choosing more than 3 years old parents, temporarily culturing in adult mussel pool, injecting the chorionic gonadotropin in female parent, culturing the female parents in individual pool, taking the seminal fluid from male parent, diluting by sodium chloride solution, injecting it in female parent twice, culturing in temporary pool until glochidium is mature, parasitizing and culturing young mussel.

The invention discloses a crossbreeding method capable of improving the high-temperature tolerance of patinopecten yesoensis. Female patinopecten yesoensis and amusium pleuronectes are used as parent scallops, and insemination is carried out at a water temperature of 18-19 DEG C, wherein the fertilization rate of sperm and egg cell is high, the embryo and larvae develop quickly, the time of developing into D-shaped larvae and juveniles is remarkably shortened, hybrid scallop seedlings with fast growth and strong stress resistance can be provided, the ability of final scallop and offspring hybrid scallop adapting to a high-temperature environment is enhanced, the death does not occur when the sea water temperature reaches 26 DEG C, and the farming range of patinopecten yesoensis can be expanded.

The artificial breeding process of wild Spanish mackerel includes capturing sexually mature parent fishes with drift net, squeezing live male fish to obtain sperm, squeezing live female fish to obtain egg cell, mixing the sperm and the egg cell in a fertilizing container with sea water via light shaking, shading the container for 3 min for artificial fertilization, washing the fertilized egg to eliminate sperm and other impurity, hatching the fertilized egg in slightly aired clean sea water at 18-22 deg.c and with salinity of 1.75-2.1 % for 40-55 hr, and culturing fish fry in clean sea water at 18-25 deg.c and with salinity of 1.75-2.1 % for 35-45 days to obtain Spanish mackerel fry. The present invention creates condition for large scale aquaculture of Spanish mackerel.

The invention relates to a method for producing haploid plant embryos, comprising providing microspores or pollen that comprise cell division inducing molecules; pollinating an embryo sac cell, in particular an egg cell, of the plant of which the haploid embryo is to be made with the microspores or pollen; allowing the microspores or pollen to discharge the cell division inducing molecules in or in the vicinity of the embryo sac cell, in particular the egg cell, to trigger division thereof to obtain a haploid plant embryo. When doubled haploid plant embryos are to be produced doubling of the chromosome number takes place at a certain stage after pollination, in particular during cell division or after obtaining the embryo. The invention further relates to the embryos thus obtained, plants regenerated therefrom and progeny thereof.

The invention discloses an automatic egg cell capturing and culturing device. The automatic egg cell capturing and culturing device comprises a culture solution supply mechanism which is arranged on a bracket and is linked by an electric motor, wherein the culture solution supply mechanism is connected with the motor through the bracket; the electric motor is connected with a rotary wheel disc connected by a bearing; injectors which penetrate through the disc surface are annularly and uniformly distributed on the rotary wheel disc; a cell capturing mechanism is arranged below the culture solution supply mechanism and comprises a base formed by a rectangular frame; a double-layer hole film embedded on the base is arranged on an enclosure of the rectangular frame; a waste liquid removal mechanism is arranged below the cell capturing mechanism and comprises a flask connected to the lower side of a three-neck container; a control mechanism comprises a control board which is respectively connected with a micro pump, the electric motor and a motor through wires. According to the device, batch capture of egg cells and liquid transfer culture automation are realized, and a technical scheme which is low in cost and easy to operate is provided for realizing in-vitro culture of egg cells, fertilization and early culture of embryos.

An object of the present invention is to efficiently establish cells, tissues, and organs capable of serving as donors for treating diseases, without eliciting immune rejection reactions, without starting with an egg cell. This object was achieved by providing a pluripotent stem cell having a desired genome. The cell was produced by treating with a reprogramming agent, producing a fusion cell of an MHC deficient stem cell with a somatic cell, or after producing a fusion cell of a stem cell with a somatic cell, removing a gene derived from the stem cell by performing genetic manipulation with a retrovirus.

Compositions and methods for producing a plant population lacking sexually derived embryos are provided. Compositions include suppression cassettes encoding polynucleotides and promoters resulting in parthenogenesis. Further provided are parthenogenesis genetic elements used to prevent sexual reproduction in self-reproducing plants.Methods include: utilizing maternal embryo defective recessive mutations which are maintained as a sterile inbred maintenance system, allowing generation of populations that are homozygous for recessive mutant alleles, but transgenically complemented. Methods include utilizing a toxin genes expressed via egg-cell specific promoters, creating a dominant, embryo-less phenotypes, non-transmittable through female gametes. Resultant hemizygous plants are transformed with egg-cell promoters driving the antidote, a pollen ablation PTU and a seed color marker for identification of transgenic seed. The generation of a plants 50% female fertile, having seed which when grown in the next generation will yield plants with 50% viable transgenic seed, and 50% non-viable embryo-less seed.

The invention discloses in-vitro fertilization liquid and a preparation method of the in-vitro fertilization liquid. By adopting the in-vitro fertilization liquid and the preparation method of the in-vitro fertilization liquid, disclosed by the invention, the technical problem that existing fertilization liquid is not suitable for the survival of sperm and egg cells or can influence the development and growth of fertilized eggs can be solved. The in-vitro fertilization liquid is a culture solution obtained by dissolving a variety of solutes into ultra-pure water by virtue of blending, wherein a basic solution solute comprises sodium chloride, potassium chloride, magnesium sulfate, taurine, sodium citrate and the like; a sterilizing agent is gentamicin; a stock solution solute is human serum albumin; the blended culture solution is filtered by adopting a filter membrane with a thickness of 0.2 microns again for performing sterilization treatment under a super clean bench. The in-vitro fertilization liquid disclosed by the invention contains a variety of organic or inorganic components which are suitable for the survival of the sperm and egg cells and are suitable for the growth and development of the fertilized eggs, thereby providing a guarantee for the cultivation of test-tube babies.

The invention discloses a universal fixed feed for grass carp and tilapia parent fish and a preparation method thereof and aims at providing a fixed feed for improving nutrition, constitution, reproductive capacity of the grass carp and tilapia parent fish, improving egg cell fertility rate, hatching rate and quality and survival rate of larval and juvenile. The universal fixed feed is composed of the following raw materials including 4-10 parts of fish meal, 1-7 parts of shrimp meal, 25-45 parts of soya bean meal, 3-8 parts of corn gluten meal, 15-25 parts of high protein flour, 2-8 parts of dried brewer's grain, 3-8 parts of mixed oil, 1-4 parts of squid ointment, 1-5 parts of soya bean lecithin, 1-3 parts of monocalcium phosphate, 0.1-2.0 parts of fine bran, 0.2-0.6 part of vitamin premix, 1-4 parts of mineral substance premix, 0.01-0.1 part of vitamin E, 0.1-0.6 part of vitamin C acetate, 0.02-0.06 part of astaxanthin, 0.1-0.3 part of choline chloride, 0.01-0.06 part of yeast cell wall, 0.02-0.06 part of antioxidant and 0.02-0.06 part of mildew preventive.

An extracted material from ovum of human being or animal is a new reagent for stem cells cloning and inducing technique. The preparing process of the material includes collecting raw material of ovum, washing, homogenating, freezing and melting, precipitating, picking top clear solution out, holding for impurity removal and loading.

The invention discloses a TCTP gene space-time specificity conditional and tissue specific gene knock-down animal model construction kit based on a recombinase recognition system, a targeting vector and a construction method thereof of the targeting vector, a mouse TCTP gene specific gene knock-down animal model and a sperm thereof, an oocyte thereof, an oosperm thereof, an embryo thereof, a filial generation thereof, a tissue thereof or a cell thereof as well as applications of the products and methods in an immunology research, researches of immunotoxicology, immunological rejection and andmechanisms thereof, researches of cancer cell apoptosis mechanisms and cancer targets, a research of targeted cancer therapy, safety evaluation, a purpose in the pharmacodynamic evaluation aspect, making of a tumor pathological model and a preparation of a disease treating medicine. By using a homologous recombination technology and an embryonic stem cell technology, a stably genetic TCTP gene knockout mouse is constructed; and therefore, embryonic death caused by knockout of the gene is avoided; and important significance in researches of the TCTP gene in cancer occurrence, progression and treatment are realized.

The invention provides an operating system for egg cell micro-injection. High-speed rotation of a glass needle, which is kept in water, is driven via vibration of a vibrating mechanism, so that an eddy current is caused in the water, subsequently, egg cells are moved and rotated, and finally a purpose of conducting egg cell injection is achieved; in comparison with existing systems that egg cellsare operated via micro-operation manipulators, the operating system provided by the invention can avoid mechanical contact with the egg cells, so that direct or indirect injury to the egg cells is avoided; and the end of the glass needle can conduct continuous rotation on the egg cells in an in-situ mode, so that the egg cells can be easily controlled within the range of a view field and observation can be facilitated; and in comparison with existing systems that the egg systems are operated within micro-fluidic chips, the operating system provided by the invention, which is free from limitation of operating tasks and the size of the egg cells, is strong in operating flexibility.

The invention relates to the technical field of assisted reproduction, and discloses a cryoprotectant and an application thereof, a sperm refrigerating fluid and a preparation method of the sperm refrigerating fluid. The sperm refrigerating fluid comprises a cryoprotectant, a composition and a basic culture solution, wherein the cryoprotectant comprises 0.3 to 1.1 mg / L of paclitaxel, 16 to 22 g / Lof trehalose, 7 to 13 g / L of cane sugar and 80 to 250 mL / L of glycerinum; the composition comprises 12 to 26 mg / L of resveratrol, 0.2 to 1.9 mg / L of reduced glutathione, 436 to 775 mg / L of lecithin, 0.5 to 1.6 mg / L of cholesterol, 9 to 17 mg / L of ATP sodium salt, 11 to 28 mg / L of heparin, 93 to 299 mg / L of phosphatidylserine and 5 to 25 mg / L of coenzyme Q10. The sperm refrigerating fluid can reduce damage of sperms in low-temperature freezing and freezing resuscitation processes, improve vitality of the sperms after freezing resuscitation, prolong survival time of the sperms after freezing resuscitation, promote sperm capacitation and enhance penetrating fertilization capacity of the sperms to egg cells. Thus, the fertilization success rate and blastocyst formation rate of the ova are improved, and finally the effect of improving the pregnancy success rate of the in-vitro fertilization assisted reproduction technology is achieved.

The invention discloses an optimized hatching breeding method for pinctada martensii seedlings. The method comprises the following steps of collection of parent pinctada martensiis, preparation beforeinsemination, parturition hastening through induction, insemination, hatching, trochophore collection and temporary breeding, larva breeding, prevention and treatment for insects and diseases, seedling adhering and seedling collection. According to the method, wild original strains are collected as the parent pinctada martensiis, by adjusting the seawater PH value and salt degree of breeding liquid, a good environment suitable for propagation of pinctada martensiis is created, and in the insemination process, the scientific proportion of ova to sperms is adopted; egg cells which are not subjected to insemination, excess sperm cells and remaining tissue cells are timely removed, the hatching water quality of the pinctada martensiis is effectively improved, the ovum washing time of the pinctada martensiis is shortened, the hatching density of oosperms is increased, and therefore the hatching rate is increased. The hatched and bred pinctada martensii seedlings are strong, the variety isgood, the breeding survival rate is high, the method is easy to implement, and the economic benefits are high.

The application discloses a non-human mammal model constructing kit with conditional gene knock-down of TCTP genes, a targeting vector, a constructing method of the targeting vector, a non-human mammal TCTP gene whole-body gene knock-down animal model, serums, an egg cell, a fertilized egg, an embryo, an offspring, tissues or cells of the animal model, application of products and the method in immunology research, research of immunotoxicology, immune rejection response and mechanism thereof, research of a cancer cell apoptosis mechanism, reach of a cancer target and cancer targeted therapy, safety evaluation and pharmacodynamic evaluation, production of a tumor pathologic model and preparation of drugs for preparing diseases based on a recombinase recognition system. The stably-inherited TCTP gene is constructed by using a homologous recombination technology and an embryonic stem cell technology for whole-body knock-down of a mouse, so that embryonic lethality caused by whole-body knock-down of the gene is avoided; the invention has important significance for researching occurrence and development of cancer and cancer treatment of the TCTP gene.

The invention discloses a fish egg cell extract and application thereof in inducing human adult cells to differentiate into pluripotent stem cells. Egg cells are obtained from a killed fish, centrifugated, filtered until the protein concentration is 4mg / ml, thus obtaining the fish egg cell extract. Ripe somatic cell primary culture cells or second-generation or fourth-generation offspring cells are obtained from each tissue of an adult; the extract is used for inducing the adult cells to be reversely differentiated into pluripotent stem cells which can provide various resources for clinic and can be used for treating various diseases. Compared with the prior art, the invention has advantages of not damaging cells, simple culturing conditions, non-toxicity, high inducing efficiency and the like, and at the same time avoids hidden safety hazard by using gene recombination technology.

The invention discloses an artificial induced spawning of Phascolosoma esculenta, which comprises the following steps of: selecting female and male parents with higher egg cell and sperm maturity, firstly conducting shady stimulation for 20-30h in a bamboo curtain, then placing the female and male parents in a net basket and conducting flowing-water stimulation for 58-66h in an induced spawning pond with surface flowing speed being 0.2-0.3m / s, wherein the distance between the bottom of the net basket and the surface of water in the induced spawning pond is 5-8cm. Since the bamboo curtain is used for shady stimulation, the parents are evenly dried in the shade, the effect is good and the induced spawning is facilitated; since the net basket is used for centralized flowing-water stimulation, the induced spawning rate and the insemination rate are higher; and ethylene diamine tetraacetic acid can chelate with heavy-metal ions in seawater and the heavy-metal ions are prevented from adversely influencing insemination and embryo development. Therefore, due to shady stimulation in the bamboo curtain and the flowing-water stimulation in the net basket, sperms and egg cells produced by the parents are more, the insemination rate reaches more than 90 percent and the average quantity of fertilized eggs obtained per kilogram of parent is 5895,500.

Methods and compositions are provided to increase the activity / level of an RKD polypeptide or an active variant or fragment thereof in an unreduced ovule plant cell that is outside of the embryo sac. In specific embodiments, such modulation of activity / level of the RKD polypeptide promotes an egg cell-like state in an unreduced ovule plant cell that is outside of the embryo sac. Such methods and compositions can employ an expression construct comprising a RKD polypeptide or active variant or fragment thereof operably linked to an ovule tissue-preferred promoter, in particular an ovule tissue-preferred promoter that is active in at least one non-gametophyte tissue in a plant ovule and is active in an unreduced cell that is outside of the embryo sac.

The invention provides an artificial breeding method for wild lampetra morii. The method comprises the following steps: capturing sexually mature parent lampreys with high activity; gently massaging and extruding the abdomen of the female parent lamprey, squeezing egg cells into a clean fertilization container, squeezing seminal fluid of the male parent lamprey on the egg cells, injecting sterile water, slightly and uniformly shaking, and standing at the temperature of 15-21 DEG C for performing artificial insemination; slightly cleaning excessive seminal fluid on the egg cells by using the sterile water; incubating the oosperm in an incubator at the temperature of 15-21 DEG C, separating the incubated lamprey juvenile fish in a constant temperature breeding box of which the bottom is paved with fine sand and which has a circulating water flow system at the temperature of 15-21 DEG C, breeding for 20-25 days, feeding yeasts in an amount of 50-100ug per fish, and feeding once a day until the wild lampetra morii fry is obtained. According to the method, the technical problem that the wild lampetra morii is difficult to breed in an artificial manner is solved, and conditions are created for large-scale lamprey breeding in labs.