TCTP gene tissue specific knock-down animal model, preparation method thereof and application thereof

Abstract

The invention discloses a TCTP gene space-time specificity conditional and tissue specific gene knock-down animal model construction kit based on a recombinase recognition system, a targeting vector and a construction method thereof of the targeting vector, a mouse TCTP gene specific gene knock-down animal model and a sperm thereof, an oocyte thereof, an oosperm thereof, an embryo thereof, a filial generation thereof, a tissue thereof or a cell thereof as well as applications of the products and methods in an immunology research, researches of immunotoxicology, immunological rejection and andmechanisms thereof, researches of cancer cell apoptosis mechanisms and cancer targets, a research of targeted cancer therapy, safety evaluation, a purpose in the pharmacodynamic evaluation aspect, making of a tumor pathological model and a preparation of a disease treating medicine. By using a homologous recombination technology and an embryonic stem cell technology, a stably genetic TCTP gene knockout mouse is constructed; and therefore, embryonic death caused by knockout of the gene is avoided; and important significance in researches of the TCTP gene in cancer occurrence, progression and treatment are realized.

Description

technical field

[0001] The present invention relates to the field of making tissue-specific gene knockdown animal models, in particular, relates to a method and application for preparing TCTP gene whole-tissue-specific knockout mice. Background technique

[0002] Cancer is one of the main chronic diseases affecting the health of residents in our country, ranking first among the causes of death in cities. In China, there are 4.29 million new cancer cases each year, which means that about 10,000 people are diagnosed with cancer every day, and about 7 people are diagnosed with cancer every minute. If the average life expectancy in China is 85 years, then the cumulative risk of cancer per person is as high as 36%. Worldwide, approximately 22% of new cancer cases and 27% of cancer deaths occurred in China. Lung cancer is the most common malignant tumor and the leading cause of cancer death in China and even in the world. With the further acceleration of industrialization and u...

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