17 results about "Gene cassette" patented technology

The present invention relates to synthetic designs or chimeric proteins for pseudotyping baculovirus (Autographa californica nuclear polyhedrosis virus) with the rabies virus glycoprotein G (gG) on its surface (Bac::gG-FL), which can be used in anti-rabies vaccine formulations. The chimeric protein is designed from a gene cassette containing gene fragments of the ectodomain of the G glycoprotein of the Pasteur strain rabies virus, a linker of 7 amino acids (GGGGSGG), as well as transmembrane (TM) and cytoplasmic (CT) regions of the gp64 baculovirus protein, with the arrangement of the sequences in the designed gene cassette being shown in figure 1.

The present disclosure provides an engineered nucleic acid sequence comprising a gene cassette, the gene cassette comprising one or more regulator genes, the regulator genes including an iadR gene, and at least three catabolic pathway genes downstream from the one or more regulator genes, the at least three catabolic pathway genes including an iadC gene, an iadD gene, and an iadE gene, wherein the engineered nucleic acid sequence is free of an iadA gene and iadB gene. The disclosure also provides bacteria cells comprising the engineered nucleic acid sequence, wherein the bacteria cell is capable of degrading indole-3-acetic acid. Methods for degrading auxin, controlling auxin, and promoting plant growth and root development are provided, comprising providing the bacteria cell and allowing the bacteria cell to interact with roots of a plant.

The present invention relates to a gene cassette expressing a fusion protein, a method for constructing same, and a method for detecting a target ligand, using same. When a target ligand binds to a target receptor ectodomain to form a dimer, a fused split fluorescent protein fragment is assembled to generate a fluorescence signal, thereby enabling rapid and simple monitoring of the presence or absence of the target ligand.

This disclosure provides nucleic acid molecules comprising a first inverted terminal repeat (ITR), a second ITR, and a gene cassette encoding a target sequence. In some embodiments, the target sequence encodes a microRNA and / or a therapeutic protein. In certain embodiments, the therapeutic protein comprises a clotting factor, a growth factor, a hormone, a cytokine, an antibody, a fragment thereof, and a combination thereof. In some embodiments, the first ITR and / or the second ITR is an ITR of a non-adeno-associated virus (AAV). This disclosure also provides methods of treating a metabolic liver disorder in a subject comprising administering to the subject the nucleic acid molecule or a polypeptide so encoded.

In a first aspect, the application relates to a plastic or rubber comprising at least one recombinant cell genetically modified to include a gene cassette, wherein the gene cassette comprises: a promo

The present invention relates to synthetic designs or chimeric proteins for pseudotyping baculovirus (Autographa californica nuclear polyhedrosis virus) with the rabies virus glycoprotein G (gG) on its surface (Bac::gG-FL), which can be used in rabies vaccine formulations. The chimeric protein is designed from a gene cassette containing genetic fragments from the ectodomain of the Pasteur variant rabies virus glycoprotein G, a 7-amino-acid connector sequence (GGGGSGG), as well as transmembrane (TM) and cytoplasmic (CT) regions of the baculoviral protein gp64, the arrangement of which in the designed gene cassette is shown in Figure 1.

A recombination vector for enhancing regeneration efficiency of hemp includes a cassette for expressing GRF3 (Growth-regulating factor 3)-GIF1 (GRF-interacting factor 1) chimeric protein or PLT5 (PLETHORA 5) protein. A method for enhancing regeneration efficiency of hemp includes transforming hemp plant cells with the recombination vector. The method may use immature hemp embryo.

Provided is a simple and efficient method of plant genome editing that does not involve the incorporation of a gene cassette for the expression system of a site-specific DNA modification protein. The method of the present invention comprises: (i) a step for mixing plant cells or a tissue containing plant cells together with a site-specific DNA modification protein and a needle-shaped inorganic compound in a liquid medium and making a disturbance to perforate the cells with the needle-shaped inorganic compound, thereby introducing the site-specific DNA modification protein into the cells; and (ii) a step for culturing the plant cells or the tissue containing the plant cells, into which the site-specific DNA modification protein has been introduced in step (i), thereby causing a DNA mutation specific to a target site in the genome of the plant cells.

Described herein is a recombinant cell genetically modified to include a gene cassette comprising a promoter and one or more of a sequence for a recombinase followed by a terminator, the recombinase-terminator sequence being flanked by recognition sites specific for the recombinase, and the cassette being activatable by an external stimulus. Also described are a method for determining the number of times the cell has been activated by an external stimulus, and plastic or rubber or a mixture of plastics or rubber including the recombinant cell, as well as a method for determining the number of times a plastic or rubber has been recycled. [Figure 1]

The present invention relates to bacteriophage vectors, and novel bacteriophage vectors comprising transgenes, in particular novel bacteriophage vectors comprising conventional mammalian transgene cassettes. The invention also extends to the use of such bacteriophage vectors as research tools, as well as their use in delivery of transgenes in various gene therapy applications, DNA and / or peptide vaccine delivery, and imaging techniques.

The present invention relates to nucleic acid molecules and uses thereof, in particular to nucleic acid molecules comprising a first reverse terminal repeat (ITR), a second ITR, and a gene cassette encoding miRNA and / or a therapeutic protein. In certain embodiments, the therapeutic protein comprises a blood coagulation factor, e.g., a FVIII polypeptide, a FIX polypeptide, or a fragment thereof. In some embodiments, the first ITR and / or the second ITR are / is an ITR of a non-adeno-associated virus (AAV). The invention also provides a method of treating a hemorrhagic condition, such as hemophilia, comprising administering to a subject the nucleic acid molecule or the polypeptide encoded thereby.

Provided is a polynucleotide. The polynucleotide comprises: an adeno-associated virus (AAV) 5' inverted terminal repeat (ITR) sequence, a recombinant AAV (rAAV) transgene cassette sequence, an AAV 3' ITR sequence, and one or both of an insulator sequence or a reverse sequence thereof linked to the 5' ITR sequence and an insulator sequence or a reverse sequence thereof linked to the 3' ITR sequence. The present disclosure also provides a vector comprising the polynucleotide, a cell, and a method for producing an rAAV vector using the polynucleotide and the vector.

The present disclosure relates to a bryophyte cell that is transformed or genetically modified to express aureusidin synthase and chalcone-4'-glycosyltransferase. The aureusidin synthase and chalcone-4'-glycosyltransferase may be expressed as a single biscistronic cassette separated by a P2A peptide or separately through insertion of two unique gene cassettes into said bryophyte cell. The expression may be driven by an exogenous promoter. The transformed bryophyte cell may be exposed to UV radiation to enhance production of exogenous flavonoids.

This invention relates to a method for increasing the yield of polyketide compounds by splitting a modular polyketide synthase gene. (1) Constructing a RedEx gene cassette for splitting the modular polyketide synthase gene; (2) Constructing a target plasmid carrying an amp-ccdB gene selection expression cassette; (3) Constructing a target plasmid carrying an amp-ccdB gene selection expression cassette. C DD-TGA-RBS-ATG- N (3) Target plasmid encoding the DD coding sequence; (4) Construction of recombinant strains; (5) Fermentation culture to obtain polyketide compounds. This invention is the first to discover that the biosynthetic efficiency of host strains carrying the split PKS gene is much higher than that of host strains carrying the natural PKS gene, which can achieve the purpose of increasing the yield of polyketide compounds. Using this method of splitting modular polyketide synthase genes, high-yield butenyl spinosad genetically engineered bacteria and high-yield avermectin genetically engineered bacteria were successfully constructed, which effectively increased the yield of butenyl spinosad and avermectin. The yield of butenyl spinosad increased by a maximum of 30 times, and the yield of avermectin increased by a maximum of 5.8 times.

UO2F2 biosensors, and related U-sensing and / or F-sensing genetic molecular components, genetic circuits, compositions, methods and systems are described, which in several embodiments can be used to detect and / or neutralize uranium and in particular bioavailable UO2F2.

The invention discloses a novel gene expression plasmid pTAEP2 capable of realizing efficient and stable overexpression in brevibacillus laterosporus (Brevibacillus laterosporus), and a preparation method of the novel gene expression plasmid pTAEP2 capable of realizing efficient and stable overexpression in brevibacillus laterosporus. The plasmid contains a toxin-antitoxin system gene cassette, so that the plasmid can stably exist in a specific non-mode host; the P2 promoter can drive high-level expression of a target gene; the RepF replicon is contained, so that the plasmid can be effectively replicated in a non-mode host; the composition contains ampicillin antibiotics and erythromycin which are used as selection markers. The invention also provides a method for target protein expression by transforming specific non-mode brevibacillus laterosporus with the pTAEP2 plasmid, the plasmid contains a TraJ element, so that the plasmid is efficiently introduced into a non-mode microbial host including brevibacillus laterosporus in a conjugational transfer manner, the gene introduction process is greatly simplified, and the production cost is reduced. And stable and high expression of a target gene is realized. The invention provides a powerful molecular biological tool for developing efficient and stable gene overexpression research and industrial application, and is beneficial to efficient gene manipulation in the non-model bacteria.

The present disclosure provides a polynucleotide comprising: an adeno-associated virus (AAV) 5'inverted terminal repeat (ITR) sequence, a recombinant adeno-associated virus (rAAV) transgene cassette sequence, an AAV 3 'ITR sequence, and one or both of an insulator sequence linked to the 5' ITR sequence, or an inverted sequence thereof, and an insulator sequence linked to the 3 'ITR sequence, or an inverted sequence thereof. The disclosure also provides vectors and cells comprising the polynucleotides, as well as methods of producing rAAV vectors using the polynucleotides and the vectors.