32results about How to "Sufficient expression" patented technology

Parvoviral capsid with incorporated Gly-Ala repeat region The present invention provides a nucleic acid construct comprising a nucleic acid sequence encoding a parvoviral VP1, VP2 and VP3 capsid proteins comprising an immuno evasion repeat sequence. In addition, the present invention provides a cell comprising such construct, a parvoviral virion comprising a capsid protein that comprises an immune evasion repeat sequence, use of that parvoviral virion in gene therapy and a pharmaceutical composition comprising such parvoviral virion.

The present invention relates to neoplasia vaccine or immunogenic composition administered in combination with other agents, such as checkpoint blockade inhibitors for the treatment or prevention of neoplasia in a subject.

The invention provides isolated KCP-like nucleic acids and their encoded proteins. The present invention provides methods and compositions relating to altering KCP-like nucleic acid and / or protein concentration and / or composition of plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.

The present invention relates to eukaryotic cells which have the ability to convert L-arabinose into D-xylulose 5-phosphate. The cells have acquired this ability by transformation with nucleotide sequences coding for an arabinose isomerase, a ribulokinase, and a ribulose-5-P-4-epimerase from a bacterium that belongs to a Clavibacter, Arthrobacter or Gramella genus. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation. The may further comprise one or more genetic modifications that increase the flux of the pentose phosphate pathway, reduce unspecific aldose reductase activity, confer to the cell the ability to directly isomerise xylose into xylulose, increase the specific xylulose kinase activity, increase transport of at least one of xylose and arabinose into the host cell, decrease sensitivity to catabolite repression, increase tolerance to ethanol, osmolarity or organic acids; and / or reduce production of by-products. The cell preferably is a cell that has the ability to produce a fermentation product such as ethanol, lactic acid, 3-hydroxy-propionic acid, acrylic acid, acetic acid, succinic acid, citric acid, amino acids, 1,3-propane-diol, ethylene, glycerol, -lactam antibiotics and cephalosporins. The invention further relates to processes for producing these fermentation products wherein a cell of the invention is used to ferment arabinose into the fermentation products.

The invention relates in general to plants, plant cells, methods of making, and methods of using plants and plant cells transformed to express an acetyltransferase that transfers an acyl group to the terminal amine of AMPA, and to plants and plant cells exhibiting resistance to AMPA in an amount which inhibits the growth of a plant or plant cell lacking a sequence encoding the acetyltransferase.

An embroidery data producing device for use with an embroidery sewing machine sewing an embroidery pattern on work cloth using a needle thread and a bobbin thread is disclosed. The embroidery data producing device produces sewing data necessary for an embroidery sewing operation of the embroidery sewing machine. The embroidery data producing device includes an image data obtaining unit that obtains image data of an original image of an embroidery pattern, a dot pattern data producing unit that produces dot pattern data representative of the embroidery pattern by a dot pattern, based on the image data, and a sewing data producing unit that produces sewing data for obtaining needle drop points forming the embroidery pattern by a pattern of the needle thread appearing in the form of dot on a back of the work cloth by an embroidery sewing operation of the embroidery sewing machine, based on the dot pattern data.

The present invention makes available rapid, effective assays for screening and identifying pharmaceutically effective compounds that specifically interact with and modulate the activity of a cellular receptor or ion channel. The subject assays enable rapid screening of large numbers of polypeptides in a library to identify those polypeptides which induce or antagonize receptor bioactivity. The subject assays are particularly amenable for identifying agonists and antagonists for orphan receptors. In particular the present invention makes available novel ligand agonists of human formyl peptide receptor like-1 (FPRL-1) receptors. These novel ligand agonists are used in the assays of the invention to identify modulators of FPRL-1 receptor.

The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and / or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

The present invention provides a polynucleotide and a method for screening and enriching a recombinant protein expressed in a eukaryotic cell, the polynucleotide comprising a tricistronic expression cassette comprising a) a promoter, b) a gene of interest (GOI), c) a reporter gene, d) a selection marker gene, e) an internal ribosome entry site (IRES) element, and f) a 2A element. Said method comprises a) transfecting or transducing suitable eukaryotic host cells with the polynucleotides of the present invention, b) culturing the eukaryotic host cells under conditions so as to express the protein of interest, the reporter protein and the selection marker protein in a cell culture selection medium suitable for selecting positively-transfected / transduced cells by means of said selection marker, and c) screening, sorting and / or enriching the cells expressing high levels of protein of interest by means of the reporter protein.

The present invention relates generally to a genetic sequence encoding a polypeptide having flavonoid 3′,5′-hydroxylase (F3′5′H) activity and to the use of the genetic sequence and / or its corresponding polypeptide thereof inter alia to manipulate color in flowers or parts thereof or in other plant tissue. More particularly, the F3′5′H has the ability to modulate dihydrokaempferol (DHK) metabolism as well as the metabolism of other substrates such as dihydroquercetin (DHQ), naringenin and eriodictyol. Even more particularly, the present invention provides a genetic sequence encoding a polypeptide having F3′5′H activity when expressed in rose or gerbera or botanically related plants. The instant invention further relates to antisense and sense molecules or RNAi-inducing molecules corresponding to all or part of the subject genetic sequence or a transcript thereof. The present invention further relates to promoters which operate efficiently in plants such as rose, gerbera or botanically related plants.

The present invention relates generally to a genetic sequence encoding a polypeptide having aromatic acyl group transfer activity and the use of the genetic sequence and / or its corresponding polypeptide thereof. More particularly, the present invention provides a genetic sequence encoding a polypeptide having aromatic acyl group transfer activity derived from Petunia, Nierembergia and Viola spp. Even more particularly, the present invention relates to a genetic sequence encoding a polypeptide having aromatic acyl group transferase activity to anthocyanidin-rutinoside. The present invention also provides a genetic sequence encoding a polypeptide having aromatic acyl group transferase activity to anthocyanidin 3-O-rutinoside. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts and reproductive tissue from such plants.

In a two-dimensional LC system configured to introduce components trapped in a trap column during a fractionation period T into a second-dimension column, separate components, and then detect the components using a mass spectrometer, a data collection unit receives a signal which indicates timing to delimit the fractionation period T, determines the first data item in the fractionation period T, adds measurement start point identification information to the first data item, and stores all the data in a data storage unit. A two-dimensional chromatogram creation unit recognizes the first data item in each fractionation period T from the read data, and create a two-dimensional chromatogram by aligning data so that the first data items will be aligned at the top along an abscissa.

A process of producing an organic compound and / or an intermediary compound as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting the culture to light, wherein the cell is capable of expressing a nucleic acid molecule, wherein the expression of the nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into the organic compound and / or into the intermediary compound and wherein the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in the culture.

The main object of the present invention is to provide a patch package structure capable of stably retaining the physiologically active ingredient in a patch during preservation of the patch. This object can be achieved by employing the following constitution.To be specific, the present invention is a patch package structure including a patch 1 and a packaging member 51 holding the patch, wherein the patch 1 includes a support 2 and an adhesive layer 3 formed on at least one surface of the support 2, the packaging member 51 includes a first package 11, the first package 11 including an acrylonitrile resin layer 12, a moisture-absorbing layer 13 formed on the acrylonitrile resin layer 12, and a moisture impermeable layer 14 formed on the moisture-absorbing layer 13, and the acrylonitrile resin layer 12 is provided on the proximal side of the patch 1.

A substantially pure and isolated DNA fragment having a nucleic acid sequence as shown in SEQ ID NO. 1 or SEQ ID NO. 2, and a substantially pure peptide having an amino acid sequence selected from the group consisting of SEQ ID NO. 3, SLS, SEQ ID NO. 4, SQD, SLL and SEQ ID NO. 5 are provided. Also provided are vaccines, antibodies and pharmaceutical compositions generated from at least one of the DNA fragments and / or peptides. Further provided are methods for detecting and / or treating rheumatoid arthritis.

A method of treating and / or preventing cancer in a subject, preferably mammalian, more preferably human, by administering in an effective amount IL-21 polypeptide, polynucleotide, vector comprising an IL-21 nucleic acid sequence encoding an IL-21 polypeptide, variants, and fragments thereof, thereby acting as an anti-cancer agent by reducing, ameliorating, and / or eliminating the cancer, and a method of treating and / or preventing cancer in a subject by co-administering the IL-21 polypeptide, polynucleotide, IL-21 vector, variant, and fragments thereof, with an immunotherapeutic and / or chemotherapeutic agent for the treatment and / or prevention of cancer in a subject.

The main object of the present invention is to provide a patch package structure capable of stably retaining the physiologically active ingredient in a patch during preservation of the patch. This object can be achieved by employing the following constitution.To be specific, the present invention is a patch package structure including a patch 1 and a packaging member 51 holding the patch, wherein the patch 1 includes a support 2 and an adhesive layer 3 formed on at least one surface of the support 2, the packaging member 51 includes a first package 11, the first package 11 including an acrylonitrile resin layer 12, a moisture-absorbing layer 13 formed on the acrylonitrile resin layer 12, and a moisture impermeable layer 14 formed on the moisture-absorbing layer 13, and the acrylonitrile resin layer 12 is provided on the proximal side of the patch 1.

In a two-dimensional LC system configured to introduce components trapped in a trap column during a fractionation period T into a second-dimension column, separate components, and then detect the components using a mass spectrometer, a data collection unit receives a signal which indicates timing to delimit the fractionation period T, determines the first data item in the fractionation period T, adds measurement start point identification information to the first data item, and stores all the data in a data storage unit. A two-dimensional chromatogram creation unit recognizes the first data item in each fractionation period T from the read data, and create a two-dimensional chromatogram by aligning data so that the first data items will be aligned at the top along an abscissa.

The present invention relates to a language learning system utilizing a component unit more segmented than a phoneme or utilizing various games. The language learning system utilizes several characters as a unit more segmented than a phoneme with respect to morphology learning, and a phoneme as a unit more segmented than a syllable with respect to pronunciation learning. Users can learn a language from the segmented unit to a larger unit such as a syllable, a word and a sentence through the learning system. In addition, the language learning system provides various games to users intending to learn a language by allowing the users to notice positive effects rather than negative effects provided by a game. The games assist users in constructing a phoneme, a syllable, a word, a sentence and the like and assists in connecting a character to a pronunciation, meaning and the like.

The present invention provides a method for generating negative-stranded segmented RNA viruses using linear expression constructs in the presence of helper virus.

A process of producing an organic compound and / or an intermediary compound as defined herein by feeding carbon dioxide to a culture of a cyanobacterial cell and subjecting the culture to light, wherein the cell is capable of expressing a nucleic acid molecule, wherein the expression of the nucleic acid molecule confer on the cell the ability to convert a glycolytic intermediate into the organic compound and / or into the intermediary compound and wherein the nucleic acid molecule is under the control of a regulatory system which responds to a change in the concentration of a nutrient in the culture.