90 results about "Petunia" patented technology

The present invention relates to isolated nucleic acid molecules which restore fertility to cytoplasmic male sterile plants and modify expression of toxic mitochondria proteins by the plant. The present invention also relates to methods of identifying a candidate plant suitable for breeding with a cytoplasmic male sterile plant and methods of identifying a candidate gene restoring fertility in plants by analyzing for the candidate plant and candidate gene, respectively, for the presence of the nucleic acid molecule of the present invention. Also disclosed are methods of producing hybrid plant seed, methods of directing gene expression to plant mitochondria, and method of expressing a gene preferentially in roots of a plant. Promoters and terminators from plant genes which restore fertility to cytoplasmic male sterile plants and modify expression of toxic mitochondria proteins are also disclosed. Finally, methods of producing plants with a cytoplasmic male sterile plant restoration system are disclosed.

The invention discloses a sexual polyploidization breeding method for tetraploid petunias. The method comprises the following steps: firstly, determining the sizes and the morphological characteristics of flower buds at a reduction mitosis prophase I of diploid petunias to grasp the optimal treatment period; carrying out inducing treatment with colchicines with optimal concentration for proper times, observing the sizes and the morphological characteristics of petunia 2n pollen and n pollen of the petunias to rapidly distinguish and identify the 2n pollen; carrying out sexual polyploidization hybridizing; and identifying ploidies of hybridized F1 plant of the petunias by a simple and easy cytology identification method. With the adoption of the breeding method, the obtained sexual polyploidization hybridized F1 not only has the characteristics of large volume of a parent polyploidy organ, but also has the inheritable characters from a diploid male parent; compared with an autotetraploid obtained by an asexual polyploidization manner, the tetraploid petunias have wider genetic diversity, and higher ornamental value and seed setting rate.

The present invention belongs to the field of molecular biology and gene technology, and is especially the nucleotide coding sequence of flavoid-3', 5'-hydroxylase gene expressed in butterfly orchid, and its application in changing flower color. The present invention relates to the recombinant expression vector including the said gene, the transgenic plant, nucleotide primer sequence for obtaining the gene, the oligonucleotide sequence probe for detecting endogenous expression mode and method of analyzing and identifying the expression mode of the endogenous gene in butterfly orchid. Transforming the gene into petunia obtains transgenic plant with differentially expressed flavoid-3', 5'-hydroxylase gene and altered flower color.

Unique fusion genes are disclosed which are useful for transforming a wide range of plants, and when used in tandem, result in a significant alteration of the plant phenotype with respect to tolerance to the stress from prolonged storage under either dark or cold conditions, or a combination of cold and dark conditions. With intact plants such as transplants, plants harboring these genes maintain the ability of recover and grow normally after returned to normal growth conditions. With isolated plant parts, such as cut flowers or foliage or fruits & vegetables, leaf tissue maintains color quality and cells maintain structural integrity during prolonged storage. Since the transgenes are only activated in response to cold temperature, normal plant growth, development, and function is not affected. The gene constructs include (1) a cold-regulated gene (COR15a) promoter to drive an ipt coding sequence that expresses IPT in the tissues of plants or plant parts exposed to a short cold induction period; and (2) a cold-regulated gene (COR15a) promoter to drive a (FAD7) coding sequence that expresses a fatty acid desaturase enzyme in the tissues of plants or plant parts exposed to a short cold induction period. Exemplary transformations include chrysanthemum, tobacco, flowering tobacco, and petunia. Double transgenic tobacco, and flowering tobacco both exhibited increased survival under prolonged cold and dark storage conditions.

The invention discloses a flaking method of petunia chromosome. The method comprises the following steps: getting a petunia young bud at 8:00-10:00 in the morning of a clear day, placing in 0.002mol.L<-1> 8-hydroxyquinoline aqueous solution for pre-treating at low temperature, transferring into a Carnoy's fluid to be fixed, performing low-permeation through 0.075mol.L<-1> potassium chloride, and then water-bathing at 60 DEG.C in 1mol.L<-1> HCl solution for decomposing, washing and then low-permeating through the distilled water, and staining through aceto-carmine staining solution, transferring the young bud onto a glass slide, cutting to get a pistil stigma part, flaking and performing microscopy. The bud size, the pretreatment method, the decomposition and staining method and time and the flaking processes of petunias with different ploidy at optimal sampling time are determined, the obtained chromosome is uniform in dispersion, easy to count and easy for performing karyotype analysis, the operation is simple and convenient, the efficiency is high, and the method has a good application prospect.

The invention relates to a petunia somatocyte cell natural mutation individual tissue culture expanding propagation method, which comprises the following steps of: step one: subculture expanding propagation of mutation plantlets of the petunia, the plantlets are taken as explants and cultured in a culture medium which is particularly confected and suitable for the growing of the plantlets so as to obtain new gemmules which obviously grow and split apart; step two: root induction and transplantation, the gemmules are reinoculated into the culture medium for inducing the root to be cultured for 60 days; in the normal situation, the rootage reaches 100%; the gemmules are moved to an acclimatization room to be acclimated for two weeks and then is transplanted into an artificial medium; after one week, the gemmules are moved into the room temperature to be grown. The method can overcome difficult problems of excellent character preservation of the autogamous sterility of the petunia and the difficult rooting of sticking, and quickly breeds and plants mutation petunia plantlets with natural mutation and stable genetic characteristic which are hard to find, and breeds the new petunia specimens with ornamental value.

The present invention relates generally to a genetic sequence encoding a polypeptide having aromatic acyl group transfer activity and the use of the genetic sequence and / or its corresponding polypeptide thereof. More particularly, the present invention provides a genetic sequence encoding a polypeptide having aromatic acyl group transfer activity derived from Petunia, Nierembergia and Viola spp. Even more particularly, the present invention relates to a genetic sequence encoding a polypeptide having aromatic acyl group transferase activity to anthocyanidin-rutinoside. The present invention also provides a genetic sequence encoding a polypeptide having aromatic acyl group transferase activity to anthocyanidin 3-O-rutinoside. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts and reproductive tissue from such plants.

The invention relates to a method for using petunia petal pigment as an acid-base indicator, which comprises the steps of extracting the purple petunia petals with hydrochloric acid methanol solution to obtain pigment solution, then calibrating the color regularity of the petunia petal pigment solution at different pH values by standard solution with the pH of 1-14, and finally adding a few drops of petunia petal pigment solution to the solution to be tested so as to predict the pH range of the solution according to the color condition.

The invention discloses a method for culturing triploid petunia. The method comprises the following steps: selecting a diploid petunia combination with strong heterosis as a crossing parent, selecting proper flower buds in the flowering period of a male parent, carrying out chemical mutagenesis by adopting mixed liquid of colchicine, oryzalin and trifluralin to generate 2n pollens, pollinating the 2n pollens to a diploid female parent stigma to obtain F1 hybridized seeds, and hybridizing n pollens the male parent of which is not subjected to chemical mutagenesis with the female parent to obtain contrast diploid F1 hybrid seeds. The adult plants developed from the F1 hybridized seeds are compared with the contrast diploid F1 hybridized filial generations in the aspects of agronomic trait and anatomy, and on this basis, the number of chromosomes can be detected so as to finally determine the triploid plant. According to the method disclosed by the invention, the breeding period is short, the operation is convenient, the mutagenesis efficiency is high and the application prospect is good.

The invention belongs to the technical field of plant variety cultivation, and specifically relates to a phalaenopsis aphrodite R3-MYBx1 gene and application thereof in flower color adjustment. An open reading frame of the gene contains a 162bp base, and a base sequence of the gene is shown as SEQ ID NO.1. The MYBx1 gene participates in a petal coloration process, but the MYBx1 gene can express both white petals and red petals; phalaenopsis aphrodite petal coloration can be affected by a plurality of transcription factors, and the MYBx1 is one of regulation factors and participates in the petal coloration process. Application of the gene in overexpression in petunia shows that the gene is related to an anthocyanin content, and the stronger expression of the gene is, the stronger anthocyanin synthesis inhibition capacity is. The MYBx1 is the gene which is researched and shown for the first time to have an obvious and specific anthocyanin degrading function. Through overexpression in plants, the gene can create novel varieties with novel petal coloration characteristics.

The invention belongs to the technical field of tree planting and particularly relates to a petunia hybrid planting method which is perfect and can accelerate growth of the petunia hybrid. The petunia hybrid planting method comprises steps as follows: 1, sowing and seedling raising: the petunia hybrid is planted in an open field after latest frost, a larger seedling with 6-8 large branches and no fewer than 50 leaves can be cultured after about 90 days, and some varieties of petunia hybrid not only meet the above requirements, but also have at least one flower; 2, transplantation: sowing and seedling raising of the petunia hybrid can be performed all the year round in protected areas of the middle and lower reaches of the Yangtze river; 3, transplantation and potting: a raising seedling dish with 288 holes is adopted for seedling raising, the seedling obtained through autumn sowing is directly transplanted to a nutrition pot with a diameter of 13 cm, and the seedling obtained through spring sowing is generally transplanted to a nutrition pot with a diameter of 10 cm; 4, cultivation and management: watering is performed in the principle that petunia hybrid is not watered when the field is not dry and is thoroughly watered once the petunia hybrid is watered.

The present invention relates to a petunia plant, seed, variety and hybrid. More specifically, the invention relates to a petunia plant having an allele which results in a petunia plant with altered flower color and / or altered flower color pattern. The invention also relates to crossing petunia plants containing the allele with petunia or Calibrachoa plants lacking the allele to produce novel types of petunia and Calibrachoa-petunia inter-generic hybrid plants.

The invention discloses an electrostatic spinning device for preparing nanofibers. The device mainly comprises a liquid spraying device, an electrostatic generator, a needle electrode, a plate electrode, a spinning pond, a liquid storage tank and a receiving curtain, wherein the liquid spraying device is fixed at the bottom of the spinning pond to be connected with the liquid storage tank, the needle electrode is arranged at the bottom of the spinning pond to be connected with the electrostatic generator, the plate electrode is arranged above the spinning pond and is grounded, and the receiving curtain is arranged below the plate electrode. During spinning, a solution is fed into the liquid spraying device from the liquid storage tank at certain pressure and certain flow rate, and forms a petunia-shaped thin liquid film at the top end of the liquid spraying device, the electrostatic generator enables a static electric field to be formed between the needle electrode and the plate electrode, jet flow is formed at the top end of the liquid film and flies off, and is deposited on the receiving curtain to form a nanofiber felt, and a solution in the spinning pond is pumped back into the liquid storage tank. The electrostatic spinning device has the benefits that the structure is simple, the cost is low, a spinning solution can be recycled, the production efficiency is high, large-scale production of the nanofibers can be realized, and requirements on the nanofibers and relevant products of industrial production are met.

The invention discloses a method for prompting petunia cuttage rooting. The method includes the following steps: step 1, selecting a new branch which grows robustly and uniformly, cutting a base to be flat, removing leaves on the lower portion, reserving 2-3 leaves on the upper portion, and making a cutting of 7-9cm in length; step 2, dipping the cutting obtained in the step 1 in a mixed solution of NAA and IBA for 3-5S, wherein concentration of NAA in the mixed solution is 300-400mg / L, and concentration of IBA in the mixed solution is 100-200mg / L; step 3, inserting the cutting obtained in the step 2 in a nutrition bowl containing a matrix, watering sufficiently for one time, maintaining temperature at 20-25 DEG C and humidity at 70-90%, and managing according to common practice, wherein the matrix is formed by mixing peat, vermiculite and sand according to a mass ratio of 1:1:1-3. The method is simple, convenient in operation, low in cost, short in period and suitable for large-scale popularization.

The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and / or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S-adenosyl-L-methionine:anthocyanin 3′-O-methyl-transferase or S-adenosyl-L-methionine:anthocyanin 3′,5′-O-methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.

The invention discloses a method for evaluating waterlogging tolerance of petunia. The method comprises the following steps of using different root seedlings of petunia as test materials, and using apotted water flooding identification method with the soil surface flooded by 2-3cm for continuously flooding for 4 days; taking phenotypic changes of the plant during flooding, including leaf color, leaf wilting degree, stem color and stem morphology as indicators of evaluation; selecting high-quality variety of petunia with high waterlogging tolerance after calculating the total score based on the graded score criteria; establishing a fast and effective waterlogging tolerance evaluation system that can objectively reflect the true status of waterlogging damages based on the changes in leaf color, leaf wilting degree, stem color and morphology, and correctly evaluating the waterlogging tolerance of different petunia strains to excavate excellent waterlogging germplasm resources.

The invention provides a cultivating method of transgenic petunia capable of removing environmental pollutants efficiently, which comprises the following steps: a vector pSLD50-6 containing a CYP2E1 gene is transferred to agrobacterium rhizogenes K599; transgenic hairy adventitious roots transformed by the CYP2E1 gene are obtained by an agrobacterium tumefaciens transgenic method; the hairy adventitious roots induce callus on a culture medium of MS + BA 1.0mg / L; the callus obtains cluster buds on a culture medium of MS + BA 1.0mg / L + NAA 0.4mg / L; and the cluster buds regenerate plants on a culture medium of MS + NAA 0.1mg / L to obtain the transgenic petunia. The petunia obtained in the invention can be used for removing indoor air pollutants of benzene, toluene and the like, and is very favorable to the physical and mental health of people, the improvement of the quality of life of people, and the construction of an ecological environment.

The invention discloses a formula feed for enhancing disease resistance of broiler breeder. The feed is prepared from the following raw materials: hulled corn, cottonseed meal, bean cake, rice bran, wheat bran, corn stalk, dried leech powder, eupatorium, bamboo juice, fish meal, carrot juice, pig liver meal, folium cortex eucommiae, liquorice, the root bark of white mulberry, Chinese chive seeds, papaya powder, dayflower, salsola collina, petunia hybrid stems and leaves, dried pumpkin, dried red dates powder, salt and a phagostimulant. The formula feed disclosed by the invention can promote growth and development, the disease-resistant and antibacterial abilities of chicken flocks are improved on the basis of guaranteeing normal growth and development of breeding hens, and the incidence rate of the chicken flocks can be effectively controlled, so that high production performance of the breeding hens can be maintained, and high-quality eggs are produced.

The invention relates to an extraction method for cleft leaf petunia volatile oil, and in particular relates to a continuous counter-current ultrasonic extraction method for cleft leaf petunia volatile oil. The method comprises the steps as follows: taking the dried cleft leaf petunia on the ground, crushing and screening via the sieve of 20 meshes, adding ethanol solution of 60-70% for 6-8 times of the weight, continuous counter-current ultrasonic extracting for 2-3h at 60-70 degrees centigrade and 40KHz, separating the solid from the liquid to obtain the extracting liquid, decompressing and concentrating the extracting liquid, using the organic solvent and anhydrous sodium sulfate for drying, decompressing and concentrating to obtain the cleft leaf petunia volatile oil. The heat sensitivity component and chemical unstable component in the volatile oil cannot be destroyed for extra high temperature through the extraction method for cleft leaf petunia volatile oil, and the extraction method is simple in technology, short in extraction time and high in yield, so that the method is suitable for industrial production.

The present invention provides a herbicide containing a coptis extract and application thereof in peanut planting in saline-alkali soil. The herbicide comprises the following raw material components: 0.5-3.5 parts of pendimethalin, 4.2-8.6 parts of a coptis extract, 3-4.6 parts of myrcene, 3.5-6.5 parts of lignosulfonate, 14-20 parts of an emulsifier and 24-40 parts of a solvent. The emulsifier is a fatty alcohol polyethyleneglycol ether. The solvent comprises water, ethanol and toluene, wherein the weight ratio of water to ethanol to toluene is 3:5:2. The application of the herbicide in peanut planting in saline-alkali soil comprises steps of: firstly, diluting the herbicide by 200-250 times; and then, spraying the diluted herbicide of 90 ml per hectacre. By using the herbicide provided by the invention, the control effects of petunia, bermudagrass, cyperus rotundus and sonchus oleraceus are 99.6-100%, 99.1-99.4%, 99.4-99.6% and 97.5-98.5%, respectively.

The invention relates to a method for preventing and controlling Petunia hybrida virus diseases through weak virus. The method is implemented according to the following steps of: 1) preparing a culture medium; 2) performing detoxification culture on the stem apexes of Petunia hybrida; 3) performing virus ELISA detection; 4) inoculating a plant with weak virus; and 5) performing tissue culture and propagation on the weak-virus plant. The method has the advantages that as a plant tissue culture technique is utilized to perform the tissue culture and propagation on the weak-virus plant, propagated tissue culture seedlings all carry the weak virus and can be continuously propagated as seeds for production, so that immunization which is required before production every year in the prior art is left out, and work efficiency is exponentially improved. The incidence of mosaic virus and similar diseases of the Petunia hybrida protected by the weak virus is lower than 3 percent, and the Petunia hybrida protected by the weak virus is healthy and strong in plant, large in flower and bright in color, so the quality of the Petunia hybrida protected by the weak virus is obviously improved.

The invention belongs to the field of plant genetic engineering and relates to an identification method and application of a petunia anther development early specific expression promoter pPhGRP. The separated promoter has a nucleotide sequence shown in the formula of SEQ ID NO: 1. A result of a quantitative PCR test on a promoter-driven petunia endogenous gene shows that the promoter-driven gene only is specifically expressed in the anther development early stage and is not expressed in other stages or in other tissues. The biological function verification and GUS staining detection results show that the promoter pPhGRP-driven reporter gene is not expressed in root, leaves and silique of arabidopsis thaliana and only is specifically expressed in the anther. The promoter pPhGRP-driven toxin gene Barnase only is specifically expressed in the petunia anther and is not expressed in other stages or in other tissues so that the male sterile line having other normally developed organs is obtained. The promoter can be used for male sterility genetic engineering, anther development regulation and control, and heterosis utilization.

The invention discloses a Chinese herbal medicine pest-repellent aqueous agent and a production process thereof. The Chinese herbal medicine pest-repellent aqueous agent is prepared by compatible extraction of Chinese herbal medicines and is characterized by being prepared from, by weight, 15-20 parts of mint, 15-20 parts of garlic, 10-15 parts of herba artemisiae scopariae, 5-8 parts of herba cymbopogonis, 5-8 parts of thyme, 3-6 parts of catmint, 2-4 parts of elderberry, 3-5 parts of rosemary, 2-3 parts of lavender, 2-3 parts of artemisia apiacea, 1-2 parts of hyssopus officinalis, 1-2 partsof basil, 1-2 parts of petunia, 0.5-1 part of salvia and herba origami. A pure Chinese herbal medicine product is provided without adding of any chemical components, natural wild jujube shell extractliquid and ethyl alcohol are adopted as solvent, and the Chinese herbal medicine pest-repellent aqueous agent has an excellent efficacy of expelling pests including thrips, plutella, plutella, spiders, acarid, stinkbugs, ants, snout beetles, field mice, artogeia rapae, aulacophora femoralis, slugs, leafhoppers, tomato hawkmoth, onion maggots, root maggots, snails, cabbage worms and the like. TheChinese herbal medicine pest-repellent aqueous agent has pest-repelling, bactericidal and microelement increasing triple efficacies and does not affect quality of agricultural products.

The invention provides a method for accelerating of petunia seedling growth. Supplementary lighting is carried out on petunias for 4 hours per day for 6-8 days before the petunias is for sell, a whole set of seedling production technique process is combined, the vegetative growth rate of petunia seedlings can be effectively improved, the period of the seedlings for sell can be shortened by 4-6 days, the production cycle is reduced by 14.3 % to 21.4%, or the quality of seedling products is improved under the condition of same selling time.

A cultivation technique for Petunia hybrida belongs to the technical field of tree planting, and particularly relates to a method for planting the Petunia hybrida. The invention provides the cultivation technique for the Petunia hybrid, and the cultivation technique is long in flowering stage and is showy in color. The cultivation technique for the Petunia hybrida includes: 1) sowing, wherein seeds of the Petunia hybrida are fine and small and do not have to be covered with soil during sowing, the seeds are scattered on a surface of fine soil, and then the seeds are sprayed with water, and in this way, the seeds can germinate; 2) transplanting, wherein sowing and seedling can be performed on the Petunia hybrida all the year round in the condition of protected areas of the lower-middle reaches of yangtze river; 3) planting, wherein a base fertilizer is added into middle-size pots, and then young seedlings can be planted in the middle-size pots after the young seedlings in small pots have two or three true leaves and root systems have developed; and 4) cultivation management, wherein the principle that watering can be performed until the soil is dry and the Petunia hybrida must be thoroughly watered, is followed all the time.

The present invention relates generally to a genetic sequence encoding a polypeptide having methyltransferase activity and the use of the genetic sequence and / or the polypeptide to modify one or more phenotypic characteristics of a plant. More particularly, the methyltransferase of the present invention acts on flavonoids, preferably wherein the flavonoid is an anthocyanin. Even more particularly, the present invention relates to a polypeptide having S-adenosyl-L-methionine:anthocyanin 3′-O-methyl-transferase or S-adenosyl-L-methionine:anthocyanin 3′,5′-O-methyltransferase activity. The present invention still further provides a genetic sequence encoding a polypeptide having methyltransferase activity derived from Petunia, Torenia Fuchsia or Plumbago or botanically related plants. The instant invention further relates to antisense and sense molecules corresponding to all or part of the subject genetic sequence as well as genetically modified plants as well as cut flowers, parts, extracts and reproductive tissue from such plants.

The invention discloses a method capable of promoting tetraploid Petunia hybrid seed germination. The method includes the steps of (1), soaking seeds in hot water at 50-60 DEG C; (2), transferring the seeds into a growth regulator 6-BA (6-benzylaminopurine) water solution for soaking; (3), soaking the seeds in water; (4), transferring the seeds into an illumination incubator for changeable-light changeable-temperature seed germination for 14-16 hours under the illumination intensity of 1000-2000 Lx and at 30-33 DEG C and for 8-10 hours in darkness at 20-25 DEG C alternately, wherein media wrapping the seeds are kept moist. Compared with the prior art, the method has the advantages that the method is simple and feasible, water permeability of tetraploid Petunia hybrid seed coats is improved through hot water soaking and seed soaking, the 6-BA breaks dormancy to promote embryonic cells to grow, and a seed metabolism process is promoted through temperature and light change; the method is capable of promoting tetraploid Petunia hybrid seed germination and is of short germination time, high germination rate and unanimous shoots, thereby being promising in application prospect.

The invention discloses a petunia-shaped protection sleeve of a plug cord. The protection sleeve comprises a flower head, a corolla and a sheath opening. The flower head is in the shape of a hollow cylinder. Protruding teeth are arranged on the inner wall. The flower head is sued for wrapping a plug net tail. The protruding teeth on the inner wall are embedded into a recess of the plug net tail and are used for fixing the protection sleeve and preventing the protection sleeve from slipping. The corolla is in the shape of a horn. The external diameter of a corolla small opening is smaller than the external diameter of the flower head. The corolla is in smooth and transition connection with the right end of the flower head. The diameter of the inner wall of the corolla small opening is smaller than the inner wall of the flower head. The corolla small opening is used for wrapping wires and in stepped connection with the inner wall of the flower head. The sheath opening is a notch which is cut in half on the flower head and the left side of the corolla. Multiple paired buckling pieces are arranged along the notch and are used for connecting or opening the opening. The sheath opening can be a single-slit opening on the flower head and the left side of the corolla. The flower head is made of materials with quite high elasticity and intensity values so the flower head can directly clamp the plug net tail. According to the invention, a problem of proneness to break caused by bending and gathering of wires can be effectively solved; the protection sleeve is not likely to be lost and slip; and the protection sleeve with a decorative appearance is convenient to use.