35 results about "Petunidin" patented technology

The invention relates to a petunidin greenhouse seedling nursing method which comprises two modes of seedbed seedling nursing and seedling dish seedling nursing, and mainly controlled conditions comprise temperature, soil porocity and the fertility of a seedbed or a seedling dish. When the method is used, the germination percent of petunidin can reach 70-80 percent, and the planting percent can reach 65-75 percent; the control for all the conditions in the method is more easily realized, and the method is simple to operate, is suitable for being used in cold areas without advanced seedling nursing conditions in winter, and greatly saves the expenses of externally purchasing fresh flowers in spring.

The invention discloses a method for obtaining regeneration plants of petunia hybrida by anther culture. The method comprises the following steps of: A) performing pretreatment and disinfecting petunia hybrida buds at low temperature; B) peeling calyx and petals of the buds obtained in the step A) by using tweezers, extracting anther, and after removing capillament of the anther completely, inoculating the anther to an inducing callus culture medium; C) transferring callus obtained by subculture in the step B) to a differential culture medium to differentiate adventitious buds, and when the adventitious buds are between 3 and 5 centimeters, stopping differential culture; and D) transferring the adventitious buds which are obtained in the step C) and are used as plantlets to a rooting culture medium to perform rooting culture until at least three adventitious roots of which the lengths are more than or equal to 2 centimeters grow on the plantlets to obtain seedlings which can be planted outside a bottle, wherein the steps A), B), C) and D) are performed in sterile environment.

The invention discloses a charge-induced sulphophile expanded adsorbent bed medium with hydrophobic nature and a preparation method thereof. In the composition of the medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off active matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the charge-induced sulphophile expanded adsorbent bed medium with the hydrophobic nature. The expanded bed adsorbing medium developed by the invention combines with three protein separation principles of hydrophobic charge induced chromatography, thiophilic chromatography and expanded bed adsorption, etc., which has the advantagesof large density of the petunidin, large protein adsorption capacity, strong specificity, mild conditions of adsorption and elution, etc., and shows the characteristic of the integration of technology and process, thus being used for high efficient separation and purification of proteins such as antibody, etc.

The invention discloses a mixed model expanded adsorbent bed medium and a preparation method thereof. In the composition of medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, and petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken asthe matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off activation matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the mixed model expanded adsorbent bed medium taking the mercapto benzimidazole and the sulfonyl group as the petunidin. The novel expanded bed adsorbing medium developed by the invention combines with a plurality of petunidin-protein interaction mechanisms such as hydrophobic interaction, charge induction action, thiophilic action, etc, to form mixed model adsorption, which has the advantages of large adsorption capacity, high selectivity, etc., thus being used for high efficient separation and purification of bioactivators such as antibody, etc.

The invention provides a preparation method for a thiophilic composite microsphere and a novel method for separating and purifying serum immunoglobulin G (IgG) with high purity and high activity in batch, which has simple and convenient operation. In the invention, ferroferric oxide particles with superparamagnetism are prepared by redox reaction, magnetic microspheres are prepared by miniemulsion polymerization technology, the full and complete coating of magnetic particles with divinylbenzene monomers is realized making use of the concentration difference of moisture repel and vinyl ester is added to carry out a second coating, and the magnetic microspheres are prepared by free radical polymerization. After full alcoholysis reaction, large quantity of active functional groups appear on the surface of the magnetic microspheres, which the thiophilic magnetic composite microsphere is formed by Michael addition reaction and grafting thiophilic petunidin such as sulfhydryl-nicotinic acid, etc., thus realizing specific recognition and separation for human immunoglobulin.

The invention relates to a PCR tubular detection kit for detecting an HCV virus antigen and a ligand thereof. The kit comprises a PCR tube of an antibody or a petunidin of a sexual human immunodeficiency virus (HCV) target molecule which is obtained by capture, detection agents of A solution and B solution for detecting a ligand or an antigen of the sexual human immunodeficiency virus (HCV), a standard product and a reference product. The invention converts a ligand signal into a nucleic acid petunidin signal by direct combination of specific oligonucleotide petunidin (specific oligonucleotide petunidin carrying signal molecules) and the ligand, and then measures the ligand by PCR augmentation for amplification and detection, so the detection kit has the characteristics for detecting the ligand with quickness, high sensitivity and quantitive measurement. The invention also discloses a preparation method for the kit and an application method of the kit for detecting sexual human immunodeficiency virus (HCV) in the specific oligonucleotide ligand.

The invention discloses a method for separating and preparing petunia-3-O-(6-O-p-coumaroyl) glucoside through extraction, macroporous resin purification, extraction, high-speed countercurrent chromatography and solid-phase extraction purification and other steps to separate and purify from grapes to obtain high-purity petunienin-3-O-(6-O-p-coumaroyl) glucoside monomer. By this method, at least 80 mg of petunienin-3-O-(6-O-p-coumaroyl) glucoside can be obtained from 10 kg of grape skins, and the purity can reach no less than 98%. The method has the advantages of simple operation, large processing capacity and good repeatability, and provides a new idea for the development and utilization of grape resources in my country.

The invention provides Tagetes patula ray florets comprising cyanidin-3-rutinoside, cyanidin-3-glucoside, petunidin-3-glucoside, and cyanidin in the lower epidermal layers. The invention also provides Tagetes plants comprising a mutant prdr1-1 allele and methods for producing a plant produced by crossing such plants with themselves or with another plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by crossing Tagetes plants comprising a mutant prdr1-1 allele. The invention further relates to parts of such plants.

The invention provides a hellebore petunidin space isomer compound and preparation and application thereof. The hellebore petunidin space isomer compound is prepared from the conventional Chinese medicinal toad skin by using macroporous resin and a two-dimensional preparation chromatographic separation technology. The compound is prepared by mainly comprising the following steps of: cutting the toad skin into pieces; extracting with ethanol; separating a total extract by using the macroporous resin to obtain a toad bufadienolides extract; and performing two-dimensional preparation chromatographic separation and purification to obtain the compound. An in-vitro experiment proves that the compound has a good tumor cell suppression effect.

The invention discloses a method for preparing petunidin-3-O-glucoside by separation. The method includes alcohol extraction concentration, macroporous resin adsorption, preparative liquid phase chromatography purification and high-speed counter-current chromatography separation, and a petunidin-3-O-glucoside monomer is prepared from blueberries with complex anthocyanin compositions by separation.Preparative liquid phase chromatography and high-speed counter-current chromatography technologies are combined for the first time, process parameters are optimized, a high-purity petunidin-3-O-glucoside monomer is prepared by separation from the blueberries, and the purity of the monomer can reach up to 99%.

The invention discloses a method for separating and preparing petunidin 3-arabinoside chloride. The method comprises alcohol extraction and concentration, macroporous resin adsorption, preparative liquid chromatography purification and high-speed counter-current chromatography separation, and a petunidin 3-arabinoside chloride monomer is isolated and prepared from blueberry raw materials with complicated composition of anthocyanin. The method combines preparative liquid chromatography and high-speed counter-current chromatography technologies for the first time, the high-purity petunidin 3-arabinoside chloride monomer is isolated and obtained from blueberries by optimizing process parameters, and the purity is up to 99%.

The invention relates to a preparing method and the application of a nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The preparation includes: the nylon membrane after being activated is modified by chitosan; the modified nylon membrane is dipped into a Reactive Blue 4 dye and added with NaCL solution after heat preservation to react, added with Na2CO3 solution for reacting continuously after temperature rising, respectively washed by hot de-ionized water, methanol solution, NaCl solution, urea solution and de-ionized water to obtain the nylon affinity membrane which takesReactive Blue 4 as a petunidin. The application includes that pawpaw powders are sampled on the nylon affinity membrane which takes Reactive Blue 4 as a petunidin, washed by Tris-HCL buffer solution and eluted by NaSCN solution to obtain pawpaw prolease; the activity of the prolease and the protein content are tested; the purification times are calculated. The method is fast, simple and convenientand has large separation capacity; the prolease extracted has the advantages of good activity, high purity as well as being suitable for scale production.