8 results about "Petunidin" patented technology

The invention discloses an anthocyanidin color developing card for qualitatively and quantitatively detecting the degradation degree of burdock medicine fishy smell and a preparation and use method of the anthocyanidin color developing card. 12 anthocyanidin extracts are distributed on the color developing card, and each anthocyanidin extract at least comprises one of the following four pairs of anthocyanidin, the four pairs of anthocyanin are respectively (a) cyanidin-3-O-beta-D-glucoside and malvidine-3-O-glucoside, (b) cyanidin-3-O-beta-D-glucoside and (c) malvidine-3-O-glucoside; (b) malvidine-3-O-galactoside and paeoniflorin-3-O-arabinoside, and (b) a mixture of malvidine-3- (c) petunidin-3-O-glucoside and peonidin-3-O-glucoside, and (c) a mixture of petunidin-3-O-glucoside and And (d) peonidin-3-O-glucoside and malvidine-3-O-galactoside, which are used as main raw materials. Qualitative and quantitative detection of the degradation degree of the fishy smell of the burdock medicine is realized, and the color developing card for identifying the degradation degree of the fishy smell of the burdock medicine can be stored for a long time and is not limited by preparation and use at present.

This invention discloses novel petunidin derivatives in black goji berries, as well as methods for extracting and using them. The extract of black goji berries can be separated by chromatography, and the novel petunidin derivatives can be obtained by several preparative separations. This invention further provides the use of novel compounds in the preparation of products for the prevention and / or treatment of inflammation, the use of novel compounds in the preparation of products for the prevention and / or treatment of glucose metabolism disorder-related disorders, and the use of novel compounds in the preparation of products for the prevention and / or treatment of lipid metabolism disorder-related disorders.

The present application belongs to the technical field of pigment preparation, and provides petunidin anthocyanins and blue pigments, and a preparation method and application thereof. The present application extracts anthocyanins from Lycium ruthenicum Murr by alcohol extraction and concentration; removes sugars, proteins and other impurities in the crude extract of Lycium ruthenicum Murr anthocyanins by using a macroporous resin; and then purifies the anthocyanin freeze-dried powder by using a preparative liquid chromatography system, and collects petunidin anthocyanins mainly composed of petunidin-3-O-(trans-p-coumaroyl) rutinoside-5-O-glucoside. The petunidin anthocyanins obtained by the present application are complexed with metal ions, and the blue pigments obtained have good color and stability. Compared with the prior art technical solution of adding polysaccharides to improve the stability of natural blue pigments, the petunidin anthocyanins of the present application can also ensure the stability of the blue pigments without adding polysaccharides, and at the same time, will not reduce the color value of the blue pigments.

The present invention provides a liquid chromatography-mass spectrometry detection method for anthocyanins in tea leaves, which belongs to the field of quality inspection technology. The method comprises: preparing a system standard working solution: weighing and dissolving the standard products of 6 anthocyanins, and then diluting them step by step to obtain a series of standard working solutions; preparing a sample solution: taking a tea sample to be tested, extracting and centrifuging it, passing the supernatant through an aqueous filter membrane, and dividing the filtered extract into two groups, one group is diluted and the other group is not diluted, and both groups of extracts are sample solutions to be tested; HPLC-MS / MS detection: injecting the series of standard working solutions and the sample solution to be tested into a liquid chromatography-mass spectrometry instrument under the same conditions for analysis, thereby determining the content of each anthocyanin in the sample; the 6 anthocyanins are: pelargonidin, cyanidin, peonidin, delphinidin, morning glory pigment, and malva pigment. This method can accurately detect the content of 6 common anthocyanin monomers in food.

The application discloses a composition for enhancing the immunity of pets and a preparation method thereof, and comprises the following raw materials in parts by weight: 25-40 parts of Echinacea purpurea powder, 5-8 parts of petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer, 35-55 parts of willow bark extract, and 60-80 parts of a carrier. The composition of the application effectively improves the immunity of pets, increases the weight of pets, and promotes the growth of pets by compounding the Echinacea purpurea powder, the petunidin-3-trans-p-coumaroylerianoside-5-glucoside monomer and the willow bark extract, and synergistic effect. Meanwhile, the beef extract as the carrier also has the effects of supplementing nutrition and enhancing constitution, and can enhance the effect of the composition of the application to a certain extent.

The invention provides a method for extracting anthocyanidin from lycium ruthenicum by using supercritical CO2, which optimizes the extraction temperature, pressure, time and entrainer to improve the extraction rate and purity of anthocyanidin. Through the analysis of a high performance liquid chromatography (HPLC), the main component in the extract is confirmed to be petunidin-3-rutinoside (trans-p-coumaroyl)-5-O-glucoside. The method can effectively improve the extraction efficiency of the lycium ruthenicum anthocyanin and maintain the biological activity of the lycium ruthenicum anthocyanin, and is suitable for the fields of foods, medicines and cosmetics.

The application discloses a new petunidin derivative furanopetanin in Lycium ruthenicum, an extraction method and application thereof, and a preparation chromatographic separation of Lycium ruthenicum extract, and the new petunidin derivative furanopetanin can be obtained through multiple preparation separations. The application further provides application of the new compound furanopetanin in preparation of products for preventing and / or treating inflammation, in preparation of products for preventing and / or treating sugar metabolism disorder related diseases, and in preparation of products for preventing and / or treating lipid metabolism disorder related diseases.

The application discloses a blackberry RubHLH gene, an expression protein thereof and application, and relates to the technical field of plant genetic engineering.The blackberry RubHLH gene disclosed by the application has a nucleotide sequence as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein thereof is as shown in SEQ ID NO.2.The application constructs an expression vector of the blackberry RubHLH gene, transforms the expression vector into tomatoes, and cultivates, screens and obtains transgenic tomato plants with significantly reduced anthocyanin substances.The RubHLH gene disclosed by the application inhibits the synthesis of anthocyanin substances such as pelargonidin, proanthocyanidin, naringenin, kaempferol-3-O-rutinoside, delphinidin-3-O-rhamnoside and petunidin-3-O-acetylglucoside.The leaf of the transgenic strain is larger, darker, wrinkled and has a shape significantly different from that of CK;the number of seeds of the fruit is reduced, and the fruit matures in advance.