35 results about "Petunidin" patented technology

A blood purification sorbent for antibody removal belongs to the technical field of biomedicine, which consists of the two parts of solid-phase carrier material and a petunidin fixed on the carrier by chemical coupling. The molecular structure of the petunidin is shown as above, wherein, one atom among A, B and C is N, the others are C; n is 0-2. The blood purification sorbent can adsorb the antibody component in plasma and autoantibodies such as rheumatoid factor, antinuclear antibody, etc. in heavy load, has limited nonspecific adsorption to other plasma components such as seralbumin, etc., and also has low preparation cost and stable physicochemical property. The material can be used as adsorption filler of a blood purification device for removing the autoantibody and immune complex in the plasma.

The invention discloses an expanded bed adsorption medium for separating antibodies by taking mercapto ehtylpyridine and sulfonyl groups as petunidins, and a preparation method. In the composition of the medium, interstitial substance is cellulose / inorganic weighting-agent composite microspheres, and the petunidins are sulfonyl groups or mercapto ehtylpyridine. The cellulose / inorganic weighting-agent composite microspheres which are used as the interstitial substance are obtained by adopting an opposite-phase suspension thermal-reproduction method; dried activation interstitial substance, the mercapto ehtylpyridine and sodium hydroxide containing ammonium persulfate are mixed for coupling to obtain the expanded bed adsorption medium for separating the antibodies by taking the mercapto ehtylpyridine and the sulfonyl groups as the petunidins. The novel expanded bed adsorption medium developed in the invention enhances the selectivity of mercapto ehtylpyridine petunidin to the antibodies through bring in the sulfonyl groups, has good adsorption separation selectivity to the antibodies, and can be used for the large-scale preparation of the antibodies.

The invention discloses a mixed model expanded adsorbent bed medium and a preparation method thereof. In the composition of medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, and petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off activation matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the mixed model expanded adsorbent bed medium taking the mercapto benzimidazole and the sulfonyl group as the petunidin. The novel expanded bed adsorbing medium developed by the invention combines with a plurality of petunidin-protein interaction mechanisms such as hydrophobic interaction, charge induction action, thiophilic action, etc, to form mixed model adsorption, which has the advantages of large adsorption capacity, high selectivity, etc., thus being used for high efficient separation and purification of bioactivators such as antibody, etc.

The invention discloses a charge-induced sulphophile expanded adsorbent bed medium with hydrophobic nature and a preparation method thereof. In the composition of the medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off active matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the charge-induced sulphophile expanded adsorbent bed medium with the hydrophobic nature. The expanded bed adsorbing medium developed by the invention combines with three protein separation principles of hydrophobic charge induced chromatography, thiophilic chromatography and expanded bed adsorption, etc., which has the advantages of large density of the petunidin, large protein adsorption capacity, strong specificity, mild conditions of adsorption and elution, etc., and shows the characteristic of the integration of technology and process, thus being used for high efficient separation and purification of proteins such as antibody, etc.

The invention provides an agarose compatible medium for purifying immune globulin of the hand-foot-and-mouth disease and a preparation method thereof. In the agarose compatible medium, agarose gel is used as a matrix, is activated by an epoxy activating agent, and is coupled with vaccine of the hand-foot-and-mouth disease which is used as petunidin, wherein the agarose gel is made of one of agarose 6FF, agarose 4FF, agarose CL-6B, agarose CL-4B, agarose 6B and agarose 4B. The agarose compatible medium can be used for purifying the immune globulin of the hand-foot-and-mouth disease, has the advantages of good medium selectivity, strong specificity, strong mechanical strength and industrial amplification benefit. Furthermore, the preparation process is simple and is easy to amplify. The medium prepared by the method is adopted for purifying the immune globulin of the hand-foot-and-mouth disease, and has simple and feasible purification method and good separation effect.

The invention relates to a petunidin greenhouse seedling nursing method which comprises two modes of seedbed seedling nursing and seedling dish seedling nursing, and mainly controlled conditions comprise temperature, soil porocity and the fertility of a seedbed or a seedling dish. When the method is used, the germination percent of petunidin can reach 70-80 percent, and the planting percent can reach 65-75 percent; the control for all the conditions in the method is more easily realized, and the method is simple to operate, is suitable for being used in cold areas without advanced seedling nursing conditions in winter, and greatly saves the expenses of externally purchasing fresh flowers in spring.

The invention discloses a method for obtaining regeneration plants of petunia hybrida by anther culture. The method comprises the following steps of: A) performing pretreatment and disinfecting petunia hybrida buds at low temperature; B) peeling calyx and petals of the buds obtained in the step A) by using tweezers, extracting anther, and after removing capillament of the anther completely, inoculating the anther to an inducing callus culture medium; C) transferring callus obtained by subculture in the step B) to a differential culture medium to differentiate adventitious buds, and when the adventitious buds are between 3 and 5 centimeters, stopping differential culture; and D) transferring the adventitious buds which are obtained in the step C) and are used as plantlets to a rooting culture medium to perform rooting culture until at least three adventitious roots of which the lengths are more than or equal to 2 centimeters grow on the plantlets to obtain seedlings which can be planted outside a bottle, wherein the steps A), B), C) and D) are performed in sterile environment.

The invention provides a method for separating petunidin in lycium ruthenicum murray through high-speed counter-current chromatography. With utilization of the high-speed counter-current chromatography and adopting of a reasonable solvent system, the high-purity petunidin is obtained from lycium ruthenicum murray; the method does requires no use of a solid-phase carrier, has no irreversible adsorption, and separates and prepares petunidin anthocyanin with no loss of samples, no pollution, high efficiency, speediness and low cost; the purity of the obtained petunidin can reach more than 97%. The method has the advantages of simple process, convenient operation, safety and good reproducibility, and can be used for industrialized production.

The invention relates to a method for synthesizing a hydrophile adsorption chromatography medium which takes gallic acid as the petunidin and the agarose gel with high concentration and high crosslinking degree as the stroma. The high concentration and high crosslinking agarose gel medium surface has high density reactionable hydroxyl group which is activated with allyl bromide followed by reacting with bromine water, and then gallic acid is added, and the prepared medium can be used as a hydrogen bond supplyer in the hydrophile adsorption reaction. The medium prepared by the synthesis method has strong rigidity, high petunidin density, high selectivity, and is fit for separating polyphenol protein, hydrophilic polypeptide and natural small organic molecules.

The invention relates to a PCR tubular detection kit for detecting an HPV virus antigen and a ligand thereof. The kit comprises a PCR tube of an antibody or a petunidin of a sexual human immunodeficiency virus (HPV) target molecule which is obtained by capture, detection agents of A solution and B solution for detecting a ligand or an antigen of the sexual human immunodeficiency virus (HPV), a standard product and a reference product. The invention converts a ligand signal into a nucleic acid petunidin signal by direct combination of specific oligonucleotide petunidin (specific oligonucleotide petunidin carrying signal molecules) and the ligand, and then measures the ligand by PCR augmentation for amplification and detection, so the detection kit has the characteristics for detecting the ligand with quickness, high sensitivity and quantitive measurement. The invention also discloses a preparation method for the kit and an application method of the kit for detecting sexual human immunodeficiency virus (HPV) in the specific oligonucleotide ligand.

The invention relates to 16alpha-methyl-3-hydroxyl-19-norpregnane-1, 3, 5-triene-20-ketone and a preparation method thereof. In the invention, degraded product of natural diosgenine petunidin 3-acetoxyl group-pregnane-5, 16-diene-20-ketone is used as the raw material, the raw material is methylated by aluminum methide before hydrolization, 1-dehydrogenation and A aromaticcyclizationofparaflins to prepare 16alpha-methyl estrone steroid compound 16alpha-methyl-3-hydroxyl-19-norpregnane-1, 3, 5-triene-20-ketone. The compound has activity suppression effect for breast cancer cellbeads MDA-MB-231, and the preparation method is characterized by easily available raw material and high yield.

The invention relates to a preparing method and the application of a nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The preparation includes: the nylon membrane after being activated is modified by chitosan; the modified nylon membrane is dipped into a Reactive Blue 4 dye and added with NaCL solution after heat preservation to react, added with Na2CO3 solution for reacting continuously after temperature rising, respectively washed by hot de-ionized water, methanol solution, NaCl solution, urea solution and de-ionized water to obtain the nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The application includes that pawpaw powders are sampled on the nylon affinity membrane which takes Reactive Blue 4 as a petunidin, washed by Tris-HCL buffer solution and eluted by NaSCN solution to obtain pawpaw prolease; the activity of the prolease and the protein content are tested; the purification times are calculated. The method is fast, simple and convenient and has large separation capacity; the prolease extracted has the advantages of good activity, high purity as well as being suitable for scale production.

The invention discloses a preparation method of an anthocyanin standard, namely petunidin-3-(trans-p-coumaroyl)-rutinoside-5-glucoside. The preparation method comprises the steps of extraction and purification of polydextran gel resin and a preparation liquid phase. According to the method, an extraction source of anthocyanin is a nightshade fruit; the nightshade fruit is easy to obtain; a proportion of the anthocyanin standard in the anthocyanin of the nightshade fruit is maximum; and the extraction cost of the anthocyanin standard is greatly lowered. At the same time, the polydextran gel resin and the preparation liquid phase are separated, purified and collected in an extraction process; the separation and purification efficiency can be significantly improved; and at last, a petunidin-3-(trans-p-coumaroyl)-rutinoside-5-glucoside standard with purity of greater than 98% can be obtained.

The invention discloses a charge-induced sulphophile expanded adsorbent bed medium with hydrophobic nature and a preparation method thereof. In the composition of the medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken as the matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off active matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the charge-induced sulphophile expanded adsorbent bed medium with the hydrophobic nature. The expanded bed adsorbing medium developed by the invention combines with three protein separation principles of hydrophobic charge induced chromatography, thiophilic chromatography and expanded bed adsorption, etc., which has the advantagesof large density of the petunidin, large protein adsorption capacity, strong specificity, mild conditions of adsorption and elution, etc., and shows the characteristic of the integration of technology and process, thus being used for high efficient separation and purification of proteins such as antibody, etc.

The invention provides a high performance liquid chromatography detection method for anthocyanin in blueberries, and belongs to the technical field of photoelectrode materials. The anthocyanin comprises delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, petunidin-3-O-glucoside, pelargonidin-3-O-glucoside, paeoniflorin 3-O-glucoside and malvidin 3-O-glucoside, wherein the flow rate of the high performance liquid chromatography is 0.8 mL / min, the detection wavelength is 520 nm, the detection temperature is 25 DEG C, the sample injection volume is 10 microliters, the detection time is 60 minutes, mobile phases: an aqueous phase B is a 0.3 wt% phosphoric acid aqueous solution, and an organic phase A is acetonitrile, and gradient elution. According to the present invention, the simultaneous determination of the six anthocyanins in the blueberry sample is achieved, the standard deviation of the peak area and the retention time is small, the detection limit and the quantification limit of the six anthocyanins are low, and the method has characteristics of high sensitivity, good precision, good reproducibility and good stability.

The invention discloses a mixed model expanded adsorbent bed medium and a preparation method thereof. In the composition of medium, matrix is a composite microsphere with fibrin / inorganic weighting agent, and petunidin is sulfonyl group and mercapto benzimidazole. The composite microsphere with fibrin / inorganic weighting agent is acquired by inverse suspension thermal regeneration and is taken asthe matrix to mix divinyl sulfone, soda buffer solution and DMSO for activation, then swab-off activation matrix, the mercapto benzimidazole and sodium hydroxide containing ammonium persulfate are mixed for coupling, thus acquiring the mixed model expanded adsorbent bed medium taking the mercapto benzimidazole and the sulfonyl group as the petunidin. The novel expanded bed adsorbing medium developed by the invention combines with a plurality of petunidin-protein interaction mechanisms such as hydrophobic interaction, charge induction action, thiophilic action, etc, to form mixed model adsorption, which has the advantages of large adsorption capacity, high selectivity, etc., thus being used for high efficient separation and purification of bioactivators such as antibody, etc.

The invention relates to a method for preparing five anthocyanin standard substances from a blueberry anthocyanin extract, which comprises the following steps: treating a blueberry anthocyanin solution to be prepared containing five anthocyanin components through an organic filter membrane, and then carrying out semi-preparative high performance liquid chromatography treatment to obtain the five anthocyanin standard substances. The five kinds of anthocyanins are delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, petunidin-3-O-glucoside, peonidin-3-O-glucoside and malvidin 3-O-glucoside. According to the method, the semi-preparative high performance liquid chromatography treatment conditions are optimized, so that different anthocyanin substances can be collected at different retention times, the method for simultaneously preparing five main anthocyanin standard substances from the blueberry anthocyanin extract is realized, the purity is high, and the yield is high; and moreover, the operation is simple and convenient, and the large-scale preparation of the anthocyanin in blueberries and the demand of the market on the anthocyanin monomers can be met.

The invention provides a preparation method for a thiophilic composite microsphere and a novel method for separating and purifying serum immunoglobulin G (IgG) with high purity and high activity in batch, which has simple and convenient operation. In the invention, ferroferric oxide particles with superparamagnetism are prepared by redox reaction, magnetic microspheres are prepared by miniemulsion polymerization technology, the full and complete coating of magnetic particles with divinylbenzene monomers is realized making use of the concentration difference of moisture repel and vinyl ester is added to carry out a second coating, and the magnetic microspheres are prepared by free radical polymerization. After full alcoholysis reaction, large quantity of active functional groups appear on the surface of the magnetic microspheres, which the thiophilic magnetic composite microsphere is formed by Michael addition reaction and grafting thiophilic petunidin such as sulfhydryl-nicotinic acid, etc., thus realizing specific recognition and separation for human immunoglobulin.

The invention provides a hellebore petunidin space isomer compound and preparation and application thereof. The hellebore petunidin space isomer compound is prepared from the conventional Chinese medicinal toad skin by using macroporous resin and a two-dimensional preparation chromatographic separation technology. The compound is prepared by mainly comprising the following steps of: cutting the toad skin into pieces; extracting with ethanol; separating a total extract by using the macroporous resin to obtain a toad bufadienolides extract; and performing two-dimensional preparation chromatographic separation and purification to obtain the compound. An in-vitro experiment proves that the compound has a good tumor cell suppression effect.

The present invention discloses a steady-state petunidin-3-O-glucoside derivative and a preparation method thereof, belongs to the technical field of compounds and preparation thereof, and solves a problem that existing anthocyanins are poor in stability. The derivative has a structural formula shown in a formula I. The preparation method is as follows: firstly stems of vitis amurensis are removedand the vitis amurensis is beaten into slurry to obtain vitis amurensis slurry liquid; then a non-thermal extraction technology is used to extract anthocyanins in the vitis amurensis slurry liquid toobtain an anthocyanin solution; then the anthocyanin solution is centrifuged, a supernatant is taken, concentration under reduced pressure, elution with a macroporous resin, eluate blending, concentration under reduced pressure, and freeze-drying are conducted to obtain purified anthocyanin; then a preparative liquid chromatography is used to separate and obtain petunia-3-O-glucoside; and finally, caffeic acid and petunidin-3-O-glucoside react, and dilution, stabilization by ultrahigh pressure, etc. are conducted to obtain the derivative. The derivative has better photothermal stability thanthe petunidin-3-O-glucoside, and has antioxidant and antitumor effects.

The invention relates to a PCR tubular detection kit for detecting an HCV virus antigen and a ligand thereof. The kit comprises a PCR tube of an antibody or a petunidin of a sexual human immunodeficiency virus (HCV) target molecule which is obtained by capture, detection agents of A solution and B solution for detecting a ligand or an antigen of the sexual human immunodeficiency virus (HCV), a standard product and a reference product. The invention converts a ligand signal into a nucleic acid petunidin signal by direct combination of specific oligonucleotide petunidin (specific oligonucleotide petunidin carrying signal molecules) and the ligand, and then measures the ligand by PCR augmentation for amplification and detection, so the detection kit has the characteristics for detecting the ligand with quickness, high sensitivity and quantitive measurement. The invention also discloses a preparation method for the kit and an application method of the kit for detecting sexual human immunodeficiency virus (HCV) in the specific oligonucleotide ligand.

The invention relates to the application of Boea crassifolia Hemsl. ex Dunn BccBcp1 gene 606bp arid correlation in cultivation drought resistance and salt-resistant plants, this gene codes 201 amino acids and compositions the multi- peptides, which has the protoplasmic membrane guide signal peptide in the amino terminal, in the middle of which has the Cu2 + union the structure territory, the carboxyl group terminal includes the plant cell wall and extends the protein unique PPR structure, which has the typical small molecular weight of the blue copper protein structure characteristic. Inducts the plant this gene obviously and enhance the plant's drought resistance, salt-resistant, height temperature-resistant forces. The construction constructed suits and expresses the carrier to the single seed leaf plant and the double seed leaf plant's BccBcp1 gene plant, which involved the start included the CacMv35s start, Ubiquitin started sub- and induction start subBcDh2, which lay between the method and through the gene gun and the agricultural bacillus which led to the tobacco, petunidin, the lawn grass and the forage grass carried on the heredity transforms, and obtained the drought resistance, salt-resistant.

The invention discloses a method for separating and preparing petunidin-3-O-(6-O-p-coumaroyl) glucoside. The method comprises the following steps: carrying out extracting, carrying out purifying by macroporous resin, carrying out extracting, carrying out high-speed counter-current chromatography, carrying out solid-phase extraction and purification and the like, and separating and purifying grapesto obtain a high-purity petunidin-3-O-(6-O-p-coumaroyl) glucoside monomer. By the method, at least 80 mg of petunidin-3-O-(6-O-p-coumaroyl) glucoside can be obtained from 10 kg of grape skin, and thepurity can reach 98% or above. The method has the advantages of simplicity in operation, high treatment capacity, high repeatability and the like, and a new idea is provided for development and utilization of grape resources in China.

The invention discloses a method for preparing petunidin-3-O-glucoside by separation. The method includes alcohol extraction concentration, macroporous resin adsorption, preparative liquid phase chromatography purification and high-speed counter-current chromatography separation, and a petunidin-3-O-glucoside monomer is prepared from blueberries with complex anthocyanin compositions by separation.Preparative liquid phase chromatography and high-speed counter-current chromatography technologies are combined for the first time, process parameters are optimized, a high-purity petunidin-3-O-glucoside monomer is prepared by separation from the blueberries, and the purity of the monomer can reach up to 99%.

The invention discloses a method for separating and preparing petunidin 3-arabinoside chloride. The method comprises alcohol extraction and concentration, macroporous resin adsorption, preparative liquid chromatography purification and high-speed counter-current chromatography separation, and a petunidin 3-arabinoside chloride monomer is isolated and prepared from blueberry raw materials with complicated composition of anthocyanin. The method combines preparative liquid chromatography and high-speed counter-current chromatography technologies for the first time, the high-purity petunidin 3-arabinoside chloride monomer is isolated and obtained from blueberries by optimizing process parameters, and the purity is up to 99%.

The invention relates to a preparing method and the application of a nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The preparation includes: the nylon membrane after being activated is modified by chitosan; the modified nylon membrane is dipped into a Reactive Blue 4 dye and added with NaCL solution after heat preservation to react, added with Na2CO3 solution for reacting continuously after temperature rising, respectively washed by hot de-ionized water, methanol solution, NaCl solution, urea solution and de-ionized water to obtain the nylon affinity membrane which takesReactive Blue 4 as a petunidin. The application includes that pawpaw powders are sampled on the nylon affinity membrane which takes Reactive Blue 4 as a petunidin, washed by Tris-HCL buffer solution and eluted by NaSCN solution to obtain pawpaw prolease; the activity of the prolease and the protein content are tested; the purification times are calculated. The method is fast, simple and convenientand has large separation capacity; the prolease extracted has the advantages of good activity, high purity as well as being suitable for scale production.