25 results about "Ribulose" patented technology

The invention discloses a method for screening and identifying a cucumber aphid-resistant gene as well as a screened gene and application thereof, and provides a brand-new method for screening and identifying the cucumber aphid-resistant gene, which is used for screening the cucumber aphid-resistant gene based on four omics technologies. The four types of omics comprise transcriptome, proteome, methylation group and metabolome before and after inoculation of aphids on cucumbers, and the cucumber aphid resistance regulation gene is screened through combined analysis, so that a new gene resource is provided for improving the aphid resistance of the cucumbers, and a new method and a new idea are provided for designing molecular genetic breeding. The CsPGD2 gene obtained through screening is an aphid resistance gene, the dynamic change of a metabolic substance 5-ribulose phosphate in a metabolome is consistent with that of a transcriptome gene CsPGD2, the aphid resistance function of the gene CsPGD2 is verified through VIGS, and the result proves that the gene CsPGD2 is an important regulation factor for the aphid resistance of cucumbers. The invention provides a new gene resource for improving the aphid resistance of cucumbers, and has a good application prospect.

The invention relates to the technical field of microbial metabolism engineering, and discloses a genetically engineered bacterium for high-yield L-histidine as well as a construction method and application of the genetically engineered bacterium. A nucleoside triphosphate pyrophosphate hydrolase coding gene mazG gene and a flagellin synthesis gene flhC gene are knocked out at the same time, an o-aminobenzoic acid phosphoribosyltransferase coding gene trpD gene and a ribulose phosphate 3-isomerase coding gene rpe gene are knocked down, and the recombinant protein is obtained. A ribose-5-phosphate isomerase gene coding rpiA gene and a lysine exponential coding gene lysE gene are overexpressed, and a promoter of 6-phosphoglucose isomerase pgi is replaced by a PfliC promoter, so that key substances for synthesis of L-histidine can be effectively accumulated, the fermentation yield of L-histidine is greatly increased, and compared with an initial strain, the yield of L-histidine is increased by 33.3%.

The invention relates to a biomass energy utilization and resource utilization technology, and aims to provide a method for improving the carbon sequestration capability of nannochloropsis oculata by domesticating and regulating a key enzyme with high ammonium salt. The method comprises the following steps: carrying out nitrogen salt gradient domestication on nannochloropsis oculata cells, and regulating and controlling the expression quantity of five key enzymes, namely H < + > transport ATP enzyme, glutamine synthetase, asparagine synthetase, carbonic anhydrase and ribulose-1, 5-diphosphate carboxylase / oxygenase, in the nannochloropsis oculata cells; the domesticated and cultured nannochloropsis oculata can independently use coked ammonium sulfate as a nitrogen source required by growth; the domesticated algal strains are subjected to transfer culture, and verification and screening are carried out aiming at the capability of fixing CO2 in flue gas and the prevention of over-acidification of the culture medium. According to the method, the utilization of coked ammonium sulfate by the nannochloropsis oculata can be enhanced without causing over-acidification of the culture medium, the microalgae culture cost can be effectively saved, and the flue gas CO2 immobilization capability of the nannochloropsis oculata is improved; a new thought is provided for large-scale breeding and cultivation by using coked ammonium sulfate and improvement of economic efficiency.

The invention discloses saccharomyces cerevisiae with high yield of salidroside as well as a construction method and application of the saccharomyces cerevisiae. According to the recombinant saccharomyces cerevisiae, ribulose-5-phosphate isomerase RKI1 and transketolase TKL1 are expressed in host bacteria, so that precursor supply is increased; reverse methyltransferase ARO2 and phenylalanine decarboxylase ARO10 are expressed at the same time, and a tyrosine branch pathway is adjusted; then, UDP-glycosyl transferase of different sources is expressed, and iterative site-specific mutagenesis is carried out on the UDP-glycosyl transferase to enhance conversion of hydroxytyrosol to salidroside; the salidroside production performance of the recombinant strain is verified on the basis of a precursor supply pathway, a tyrosine regulation pathway and key enzyme screening, and the salidroside production capacity of the saccharomyces cerevisiae is further improved.

The present application relates to the field of plant genetic breeding, and particularly relates to a kind of evaluation method of asparagus yield. Including the following steps: S1, obtaining young plant period asparagus;S2, measures i variety of young plant period asparagus following parameters: photosynthetic rate A, ribulose-1,5-bisphosphate carboxylase / oxygenase activity B, leaf dry weight per unit area C, acid invertase activity D and root crown ratio E;The measurement sample of the acid invertase activity D is the tender stem of young plant period asparagus at 10-30cm from top;S3, according to the formula, the evaluation value is calculated, and the evaluation value is used to evaluate the yield of asparagus. The evaluation value obtained by using the above measurement index and calculation formula, and the significance level obtained by SPSS software is used to evaluate the yield of asparagus. The yield potential of asparagus can be comprehensively evaluated from the genetic point of view in a short time. Using the evaluation method of the present application, high-yield asparagus varieties can be selected in a short time without tracking for many years.

The present invention relates to host cells and their use wherein the host cells are capable of producing D-ribulose and incapable of or have a reduced capability of converting D-ribulose to a molecule other than ribitol, wherein the host cells comprise a heterologous nucleic acid sequence encoding a polypeptide capable of converting D-ribulose to ribitol with a cofactor preference for NADPH.

Methods and systems are provided for the purification of native and recombinant Ribulose- 1,5- bisphosphate Carboxylase / Oxygenase (RuBisCO) from cellular lysates using liquid-liquid phase separation (LLPS). The invention enables scalable and cost-effective extraction of highly pure RuBisCO complexes from plant and bacterial sources while maintaining functional protein integrity. The methods and compositions disclosed herein address key obstacles to RuBisCO isolation and offer commercial advantages for protein production and utility in nutritional formulations.

The present disclosure relates to a dry chloroplast composition comprising chloroplasts isolated from Fabaceae family plants. The composition has a moisture content of less than about 8%. The composition has at least one ratio chosen from:a ratio of chlorophyll / fructose 1,6-biphosphatase (FBPP) of about 50 to about 130, the ratio of chlorophyll / FBPP beingchlorophyll⁢ in⁢ (mg / g)FBPP⁢ in⁢ (intensity / mg⁢ powder)×1⁢0⁢0⁢0⁢0⁢0⁢0⁢0, The FBPP intensity being measured by immunoblot,a ratio of Rubisco / chlorophyll of about 25 to about 65, the ratio of Rubisco / chlorophyll being(R⁢u⁢bisco⁢ in⁢ intensityc⁢h⁢l⁢o⁢r⁢o⁢phyll⁢ in⁡(mg / g)) / 1000, wherein the Rubisco intensity being measured by immunoblot,a ratio of Rubisco / chlorophyll of about 2.5 ng / mg to about 6.5 ng / mg, the ratio of Rubisco / chlorophyll being(R⁢u⁢bisco⁢ in⁢ ng / mg⁢ powderc⁢h⁢l⁢o⁢r⁢o⁢phyll⁢ in⁡(mg / g)) / 1000, anda ratio of Rubisco / FBPP of about 18 to about 100, the ratio of Rubisco / FBPP being(R⁢u⁢bisco⁢ in⁢ ng / mg⁢ powderFBPP⁢ in⁢ intensity / mg)×100.

The invention relates to a recombinant pichia pastoris strain for producing coupled xylitol by NADPH (nicotinamide adenine dinucleotide phosphate) dependent growth and a construction method of the recombinant pichia pastoris strain, and belongs to the technical field of microbial fermentation. The recombinant pichia pastoris strain provided by the invention contains an NADPH dependent xylitol dehydrogenase gene, a sugar phosphatase gene, a D-arabinol-4-dehydrogenase gene, a fructose-1, 3, 4-triazole-1, 3, 4-triazole-1, 3-triazole-1, 3-triazole-1, 3-triazole-1, 3- The gene sequence is as follows: a 1, 6-diphosphatase gene, a phosphoketolase gene, a phosphate transacetylase gene, a glucose-6-phosphate dehydrogenase gene, a 6-phosphogluconolactonase gene, a 6-phosphogluconate dehydrogenase gene and a ribulose-5-phosphate epimerase gene; gene for expressing xylulokinase is knocked out or down-regulated: NADH dependent type xylitol dehydrogenase gene, ribose-5-phosphate isomerase gene, glutamate dehydrogenase gene, phosphofructokinase gene and phosphoglucose isomerase gene. The yield of xylitol produced by fermentation of the strain is far higher than that in the prior art.

This invention discloses an engineered bacterial strain for producing xylitol using glucose as the sole carbon source, its construction method, and its applications, belonging to the field of genetic engineering. Using *Escherichia coli* as the starting strain, this invention optimizes the metabolic pathway by knocking out the 2-keto-3-deoxy-6-phosphate glucuronide gene (eda), knocking out the 6-phosphoglucuronide dehydratase gene (edd), and weakening the glucose phosphoisomerase gene (pgi), thus concentrating the carbon flow in the pentose phosphate pathway. Furthermore, it optimizes the metabolic pathway by knocking out or overexpressing endogenous genes such as the ribulokinase gene (araB). Subsequently, the synthesis of xylitol from glucose was experimentally tested by expressing a recombinant expression plasmid containing 2-arabinol dehydrogenase, 4-arabinol dehydrogenase, and xylitol dehydrogenase. This invention, through optimized metabolic pathways, efficiently converts glucose to xylitol without the need for glycerol addition, and significantly increases the yields of both the precursor arabinol and the product xylitol, demonstrating promising production prospects.

There is provided an aqueous personal care rinse-off formulation, the aqueous personal care rinse-off formulation comprising: a dermatologically acceptable aqueous vehicle; a dermatologically acceptable cleansing surfactant, wherein the dermatologically acceptable cleansing surfactant comprises an anionic surfactant; and an alkyl polysaccharide thickener; wherein the alkyl polysaccharide thickener comprises from 50% to 100% by weight, based on the weight of the alkyl polysaccharide thickener, of a linear alkyl polysaccharide of formula (A), R-O-(S) nH (A) wherein R is a linear C9-15 alkyl group; wherein n is an average value from 1 to 2.5; wherein S is a monosaccharide selected from the group consisting of ribulose, xylulose, ribose, arabinose, xylose, lyxose and mixtures thereof; and wherein 70% to 100% by weight, based on the weight of monosaccharide S in the linear alkyl polysaccharide of formula A, of the monosaccharide S is xylose; wherein the aqueous personal care rinse-off formulation comprises lt based on the weight of the aqueous personal care rinse-off formulation; 0.01% by weight of an alkyl sulfate surfactant and an alkyl ether sulfate surfactant.

An L-rhamnose isomerase which can be obtained from a microorganism belonging to the genus Erwinia, has a subunit of a molecular mass of about 48 kDa as determined by SDS-PAGE, and has the substrate specificity of (A) and (B) described below. (A) has an isomerase activity which recognizes and reacts with CHO group at C1 and OH group at C2 of an aldose, converts CHO group at C1 into OH group, and converts OH group at C2 into CO group, or recognizes and reacts with OH group at C1 and CO group at C2 of a ketose, converts OH group at C1 into CHO group, and converts CO group at C2 into OH group. (B) has an activity of catalyzing isomerization between L-rhamnose and L-rhamnulose, between L-lyxose and L-xylulose, between L-mannose and L-fructose, between D-ribose and D-ribulose, between L-talose and L-tagatose, and between D-allose and D-psicose.

There is provided an aqueous personal care rinse-off formulation, the aqueous personal care rinse-off formulation comprising: a dermatologically acceptable aqueous vehicle; a dermatologically acceptable cleansing surfactant, wherein the dermatologically acceptable cleansing surfactant comprises an anionic surfactant; and an alkyl polysaccharide thickener; wherein the alkyl polysaccharide thickener comprises from 50% to 100% by weight, based on the weight of the alkyl polysaccharide thickener, of a linear alkyl polysaccharide of formula A, R-O-(S) nH (A) wherein R is a linear C9-15 alkyl group; wherein n is an average value from 1 to 2.5, and wherein S is a monosaccharide selected from the group consisting of ribulose, xylulose, ribose, arabinose, xylose, lyxose and mixtures thereof; and wherein 70% to 100% by weight, based on the weight of monosaccharide S in the linear alkyl polysaccharide of formula A, of the monosaccharide S is xylose.

The invention discloses a microalgae carbon sequestration system and a microalgae carbon sequestration method thereof, and belongs to the technical field of microalgae carbon sequestration. The system comprises purified ribulose-1, 5-diphosphate carboxylation oxidase (Rubisco), purified ribulose-1, 5-diphosphate carboxylation oxidase (Rubisco), purified ribulose-1, 5-diphosphate carboxylation oxidase (Rubisco), purified ribulose-1, 5-diphosphate carboxylation oxidase (Rubisco) The invention discloses a chitosan-silicon dioxide hybrid gel microsphere for immobilizing ribulose-1, 5-diphosphate carboxylation oxidase, the particle size of the chitosan-silicon dioxide hybrid gel microsphere is 150-250 microns, the porosity is 40-60%, the pore diameter is 5-20 nm, and carbonic anhydrase is optionally contained in the chitosan-silicon dioxide hybrid gel microsphere; the living microalgae cells are suspended in the culture solution, and the living microalgae cells can freely enter and exit from the peripheral area of the chitosan-silicon dioxide hybrid gel microspheres and cannot penetrate through pore channels of the chitosan-silicon dioxide hybrid gel microspheres; and the photobioreactor is used for accommodating a culture solution and providing illumination for the living microalgae cells. According to the invention, the increase of microalgae biomass can be realized, and the efficiency of fixing CO2 by microalgae cells is improved.

The present invention relates to a method for identifying a compound that specifically modulates the activity of neutrophils by modulating at least one of the features of expression, quantity, stability, and / or biological activity of the protein ribulose-5-phosphate epimerase (RPE) in a mammalian cell. Neutrophil hyperactivity causes and / or is associated with inflammation, tissue repair processes, the healing of injuries or damage, autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), systemic sclerosis (SSc), multiple sclerosis (MS), and type 1 diabetes (T1D), or innate immunity, such as bacterial or viral infections, cancer, cardiovascular diseases, and aging.Further aspects of the invention relate to a screening method for screening for a compound, a pharmaceutical composition and a method for preventing or treating a disease or condition caused by hyperactivity of neutrophilic granulocytes.

The invention discloses recombinant escherichia coli capable of increasing the yield of L-threonine and a preparation method of the recombinant escherichia coli, and belongs to the technical field of biology. The method comprises the following steps: firstly, by taking L-threonine production strain escherichia coli LMT4 constructed in an earlier stage of a laboratory as a genetic modification basis, constructing a Kalvin cycle by introducing ribulose diphosphate carboxylase mutant derived from rhodospirillum rubrum and ribulose phosphate kinase mutant derived from rhodopseudomonas sphaeroides; the carbon loss is reduced; the L-threonine production performance of the strain is improved. On the other hand, the glyceraldehyde-3-phosphate dehydrogenase mutant derived from thermotoga maritime and thermophilic thermotoga is innovatively introduced. The cofactor competition pressure in the catalysis step of aspartate semialdehyde dehydrogenase and homoserine dehydrogenase in the L-threonine synthesis route is effectively relieved, the production intensity of the strain is further improved, and the method has certain significance in promoting the application of escherichia coli in the field of biosynthesis of L-threonine.

The invention discloses a method for preparing L-arabinose, and belongs to the technical field of preparation of L-arabinose. The invention particularly relates to a method for preparing L-arabinose by utilizing in-vitro biotransformation, which comprises the following steps: converting 6-phosphogluconic acid into D-ribulose 5-phosphoric acid by adopting 6-phosphogluconic acid dehydrogenase; the method comprises the following steps: converting D-ribulose 5-phosphoric acid into D-xylulose 5-phosphoric acid by adopting D-ribulose 5-phosphoric acid 3-epimerase; the method comprises the following steps: converting D-xylulose 5-phosphoric acid into L-ribulose 5-phosphoric acid by adopting L-ribulose 5-phosphoric acid 4-epimerase; under the action of isomerase and dephosphorizing enzyme, the L-ribulose 5-phosphoric acid is converted into the L-arabinose. The method is a brand-new biological method for preparing the L-arabinose, the L-arabinose is obtained with higher yield and higher purity, the purification process is simple, the production cost of the L-arabinose can be reduced, and the preparation process is environment-friendly.

The invention provides a root system accelerant as well as a preparation method and application thereof, and relates to the technical field of plant growth accelerants. The root system accelerant is prepared from a humic acid material as a raw material through the steps of oxidative degradation, potassium hydroxide treatment, filtering, drying and the like. The root system accelerant can improve the content of growth hormone and gibberellin in corn, improve the activity of ribulose-1, 5-diphosphate carboxylase / oxygenase in corn, avoid the inhibition of chemical fertilizers on root systems, promote the growth of crops and improve the yield.

The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.

The invention relates to a ribose-5-phosphate isomerase B mutant and application of the ribose-5-phosphate isomerase B mutant in preparation of rare sugar, and belongs to the technical field of biological engineering. The invention provides an ochrobactrum ribose-5-phosphate isomerase B mutant with relatively high activity and stability, a coding gene of the ochrobactrum ribose-5-phosphate isomerase B mutant, a recombinant expression vector containing a gene sequence, a recombinant expression transformant, a preparation method of a mutant enzyme preparation, and practical application of transforming biomass-derived D-xylose by using the mutant. According to the method, low-added-value biomass monosaccharide such as D-xylose can be converted into high-value rare sugar compounds such as D-xylulose and D-ribulose through a single-enzyme or multi-enzyme cascade reaction, the range of substrates and products is expanded, and the method has a wide application prospect.

For the purpose of producing plants having improved growth capacity (plant individual size, growth rate, etc.), the present invention addresses the problem of producing rubisco that improves the plant growth capacity. Specifically, the present invention is a method for producing a plant having improved growth capacity. The method includes introducing, into the rbcL gene sequence of the plant, a base substitution that mutates the 309th methionine of the large subunit (RbcL) of ribulose-1,5-bisphosphate carboxylase / oxygenase (rubisco) of the plant to a different amino acid and / or a base substitution that mutates the 397th aspartic acid of the RbcL of the plant to a different amino acid.

The invention discloses a method for extracting ribulose-1, 5-diphosphate carboxylase / oxygenase (RuBisCO) from plant leaves, and belongs to the field of plant protein extraction. Comprising the following steps: (1) mixing plant leaves with water, adding sodium pyrosulfite and cross-linked polyvinylpyrrolidone, pulping, crushing, stirring and centrifuging to obtain a protein crude extract; (2) adjusting the pH value of the protein crude extract, heating, centrifuging, and carrying out suction filtration to obtain a permeate; (3) carrying out ultrafiltration on the permeate to obtain a protein concentrated solution; and (4) drying the protein concentrated solution to obtain RuBisCO protein powder. The plant leaves are at least one of green leaves such as medicago sativa and spinach. The mass ratio of the plant leaves to the water is 1: (1-49); the addition amount of the sodium pyrosulfite is 0.2%-2% of the total mass of the plant leaves and the water; the addition amount of the cross-linked polyvinylpyrrolidone is 0.5%-3% of the total mass of the plant leaves and the water. The extraction method provided by the invention can achieve the effects of high protein content and recovery rate, simple operation process and cost saving.

Described herein are genelight cultures and extracts and methods of making and using thereof. In one aspect, the method of making a genelight culture or extract includes the steps of (a) making a DNA construct containing genes for producing a heat shock protein, RuBisCO large subunit 1, tonB, phosphoenol pyruvate carboxykinase, phosphoenol pyruvate carboxylase, hydrogenase, p-type ATPase, and, in some aspects, enhanced green fluorescent protein, (b) introducing the DNA construct into host microbial cells via transformation or transfection, and (c) culturing the microbial cells to produce the genelight cultures and extracts. The compositions of these cultures and extracts can be tailored to have specific properties such as the ability to provide power to a light emitting diode. The cultures and extracts have further uses including enhancing the growth of plants and as supplemental nutrients of cultures of industrially important microorganisms. The cultures and extracts further have UV-protective properties.

The invention discloses a method for producing L-aromatic amino acid by improving the utilization efficiency of carbon atoms. In order to break through the carbon efficiency bottleneck in aromatic amino acid production, a rubisco gene (encoding 1, 5-diphosphate carboxylase / oxygenase) from rhodospirillum rubrum and a prk gene (encoding ribulose phosphate kinase) from spinach are integrated into pseudogene loci of ytfI and ygaQ of an escherichia coli genome, a Kalvin circulation path is constructed, and the carbon efficiency bottleneck in aromatic amino acid production is broken through. The method aims at recovering CO2 and regenerating 3-phosphoglyceric acid to enter central carbon metabolism, and promoter optimization is combined, so that the utilization efficiency of carbon atoms is further improved.

Provided are ribulose-phosphate 3-epimerase, a microorganism and a composition, each including the ribulose-phosphate 3-epimerase, and a method of producing psicose-6-phosphate or psicose using the same.