10 results about "Ribulose" patented technology

The invention discloses saccharomyces cerevisiae with high yield of salidroside as well as a construction method and application of the saccharomyces cerevisiae. According to the recombinant saccharomyces cerevisiae, ribulose-5-phosphate isomerase RKI1 and transketolase TKL1 are expressed in host bacteria, so that precursor supply is increased; reverse methyltransferase ARO2 and phenylalanine decarboxylase ARO10 are expressed at the same time, and a tyrosine branch pathway is adjusted; then, UDP-glycosyl transferase of different sources is expressed, and iterative site-specific mutagenesis is carried out on the UDP-glycosyl transferase to enhance conversion of hydroxytyrosol to salidroside; the salidroside production performance of the recombinant strain is verified on the basis of a precursor supply pathway, a tyrosine regulation pathway and key enzyme screening, and the salidroside production capacity of the saccharomyces cerevisiae is further improved.

The present invention relates to host cells and their use wherein the host cells are capable of producing D-ribulose and incapable of or have a reduced capability of converting D-ribulose to a molecule other than ribitol, wherein the host cells comprise a heterologous nucleic acid sequence encoding a polypeptide capable of converting D-ribulose to ribitol with a cofactor preference for NADPH.

Methods and systems are provided for the purification of native and recombinant Ribulose- 1,5- bisphosphate Carboxylase / Oxygenase (RuBisCO) from cellular lysates using liquid-liquid phase separation (LLPS). The invention enables scalable and cost-effective extraction of highly pure RuBisCO complexes from plant and bacterial sources while maintaining functional protein integrity. The methods and compositions disclosed herein address key obstacles to RuBisCO isolation and offer commercial advantages for protein production and utility in nutritional formulations.

This invention discloses an engineered bacterial strain for producing xylitol using glucose as the sole carbon source, its construction method, and its applications, belonging to the field of genetic engineering. Using *Escherichia coli* as the starting strain, this invention optimizes the metabolic pathway by knocking out the 2-keto-3-deoxy-6-phosphate glucuronide gene (eda), knocking out the 6-phosphoglucuronide dehydratase gene (edd), and weakening the glucose phosphoisomerase gene (pgi), thus concentrating the carbon flow in the pentose phosphate pathway. Furthermore, it optimizes the metabolic pathway by knocking out or overexpressing endogenous genes such as the ribulokinase gene (araB). Subsequently, the synthesis of xylitol from glucose was experimentally tested by expressing a recombinant expression plasmid containing 2-arabinol dehydrogenase, 4-arabinol dehydrogenase, and xylitol dehydrogenase. This invention, through optimized metabolic pathways, efficiently converts glucose to xylitol without the need for glycerol addition, and significantly increases the yields of both the precursor arabinol and the product xylitol, demonstrating promising production prospects.

The invention discloses a microalgae carbon sequestration system and a microalgae carbon sequestration method thereof, and belongs to the technical field of microalgae carbon sequestration. The system comprises purified ribulose-1, 5-diphosphate carboxylation oxidase (Rubisco), purified ribulose-1, 5-diphosphate carboxylation oxidase (Rubisco), purified ribulose-1, 5-diphosphate carboxylation oxidase (Rubisco), purified ribulose-1, 5-diphosphate carboxylation oxidase (Rubisco) The invention discloses a chitosan-silicon dioxide hybrid gel microsphere for immobilizing ribulose-1, 5-diphosphate carboxylation oxidase, the particle size of the chitosan-silicon dioxide hybrid gel microsphere is 150-250 microns, the porosity is 40-60%, the pore diameter is 5-20 nm, and carbonic anhydrase is optionally contained in the chitosan-silicon dioxide hybrid gel microsphere; the living microalgae cells are suspended in the culture solution, and the living microalgae cells can freely enter and exit from the peripheral area of the chitosan-silicon dioxide hybrid gel microspheres and cannot penetrate through pore channels of the chitosan-silicon dioxide hybrid gel microspheres; and the photobioreactor is used for accommodating a culture solution and providing illumination for the living microalgae cells. According to the invention, the increase of microalgae biomass can be realized, and the efficiency of fixing CO2 by microalgae cells is improved.

The present invention relates to a method for identifying a compound that specifically modulates the activity of neutrophils by modulating at least one of the features of expression, quantity, stability, and / or biological activity of the protein ribulose-5-phosphate epimerase (RPE) in a mammalian cell. Neutrophil hyperactivity causes and / or is associated with inflammation, tissue repair processes, the healing of injuries or damage, autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), systemic sclerosis (SSc), multiple sclerosis (MS), and type 1 diabetes (T1D), or innate immunity, such as bacterial or viral infections, cancer, cardiovascular diseases, and aging.Further aspects of the invention relate to a screening method for screening for a compound, a pharmaceutical composition and a method for preventing or treating a disease or condition caused by hyperactivity of neutrophilic granulocytes.

For the purpose of producing plants having improved growth capacity (plant individual size, growth rate, etc.), the present invention addresses the problem of producing rubisco that improves the plant growth capacity. Specifically, the present invention is a method for producing a plant having improved growth capacity. The method includes introducing, into the rbcL gene sequence of the plant, a base substitution that mutates the 309th methionine of the large subunit (RbcL) of ribulose-1,5-bisphosphate carboxylase / oxygenase (rubisco) of the plant to a different amino acid and / or a base substitution that mutates the 397th aspartic acid of the RbcL of the plant to a different amino acid.

The invention discloses a method for extracting ribulose-1, 5-diphosphate carboxylase / oxygenase (RuBisCO) from plant leaves, and belongs to the field of plant protein extraction. Comprising the following steps: (1) mixing plant leaves with water, adding sodium pyrosulfite and cross-linked polyvinylpyrrolidone, pulping, crushing, stirring and centrifuging to obtain a protein crude extract; (2) adjusting the pH value of the protein crude extract, heating, centrifuging, and carrying out suction filtration to obtain a permeate; (3) carrying out ultrafiltration on the permeate to obtain a protein concentrated solution; and (4) drying the protein concentrated solution to obtain RuBisCO protein powder. The plant leaves are at least one of green leaves such as medicago sativa and spinach. The mass ratio of the plant leaves to the water is 1: (1-49); the addition amount of the sodium pyrosulfite is 0.2%-2% of the total mass of the plant leaves and the water; the addition amount of the cross-linked polyvinylpyrrolidone is 0.5%-3% of the total mass of the plant leaves and the water. The extraction method provided by the invention can achieve the effects of high protein content and recovery rate, simple operation process and cost saving.

The invention discloses a method for producing L-aromatic amino acid by improving the utilization efficiency of carbon atoms. In order to break through the carbon efficiency bottleneck in aromatic amino acid production, a rubisco gene (encoding 1, 5-diphosphate carboxylase / oxygenase) from rhodospirillum rubrum and a prk gene (encoding ribulose phosphate kinase) from spinach are integrated into pseudogene loci of ytfI and ygaQ of an escherichia coli genome, a Kalvin circulation path is constructed, and the carbon efficiency bottleneck in aromatic amino acid production is broken through. The method aims at recovering CO2 and regenerating 3-phosphoglyceric acid to enter central carbon metabolism, and promoter optimization is combined, so that the utilization efficiency of carbon atoms is further improved.