5 results about "Ribulose" patented technology

Methods and systems are provided for the purification of native and recombinant Ribulose- 1,5- bisphosphate Carboxylase / Oxygenase (RuBisCO) from cellular lysates using liquid-liquid phase separation (LLPS). The invention enables scalable and cost-effective extraction of highly pure RuBisCO complexes from plant and bacterial sources while maintaining functional protein integrity. The methods and compositions disclosed herein address key obstacles to RuBisCO isolation and offer commercial advantages for protein production and utility in nutritional formulations.

This invention discloses an engineered bacterial strain for producing xylitol using glucose as the sole carbon source, its construction method, and its applications, belonging to the field of genetic engineering. Using *Escherichia coli* as the starting strain, this invention optimizes the metabolic pathway by knocking out the 2-keto-3-deoxy-6-phosphate glucuronide gene (eda), knocking out the 6-phosphoglucuronide dehydratase gene (edd), and weakening the glucose phosphoisomerase gene (pgi), thus concentrating the carbon flow in the pentose phosphate pathway. Furthermore, it optimizes the metabolic pathway by knocking out or overexpressing endogenous genes such as the ribulokinase gene (araB). Subsequently, the synthesis of xylitol from glucose was experimentally tested by expressing a recombinant expression plasmid containing 2-arabinol dehydrogenase, 4-arabinol dehydrogenase, and xylitol dehydrogenase. This invention, through optimized metabolic pathways, efficiently converts glucose to xylitol without the need for glycerol addition, and significantly increases the yields of both the precursor arabinol and the product xylitol, demonstrating promising production prospects.

The present invention relates to a method for identifying a compound that specifically modulates the activity of neutrophils by modulating at least one of the features of expression, quantity, stability, and / or biological activity of the protein ribulose-5-phosphate epimerase (RPE) in a mammalian cell. Neutrophil hyperactivity causes and / or is associated with inflammation, tissue repair processes, the healing of injuries or damage, autoimmune diseases such as systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), systemic sclerosis (SSc), multiple sclerosis (MS), and type 1 diabetes (T1D), or innate immunity, such as bacterial or viral infections, cancer, cardiovascular diseases, and aging.Further aspects of the invention relate to a screening method for screening for a compound, a pharmaceutical composition and a method for preventing or treating a disease or condition caused by hyperactivity of neutrophilic granulocytes.

For the purpose of producing plants having improved growth capacity (plant individual size, growth rate, etc.), the present invention addresses the problem of producing rubisco that improves the plant growth capacity. Specifically, the present invention is a method for producing a plant having improved growth capacity. The method includes introducing, into the rbcL gene sequence of the plant, a base substitution that mutates the 309th methionine of the large subunit (RbcL) of ribulose-1,5-bisphosphate carboxylase / oxygenase (rubisco) of the plant to a different amino acid and / or a base substitution that mutates the 397th aspartic acid of the RbcL of the plant to a different amino acid.

The invention discloses a method for extracting ribulose-1, 5-diphosphate carboxylase / oxygenase (RuBisCO) from plant leaves, and belongs to the field of plant protein extraction. Comprising the following steps: (1) mixing plant leaves with water, adding sodium pyrosulfite and cross-linked polyvinylpyrrolidone, pulping, crushing, stirring and centrifuging to obtain a protein crude extract; (2) adjusting the pH value of the protein crude extract, heating, centrifuging, and carrying out suction filtration to obtain a permeate; (3) carrying out ultrafiltration on the permeate to obtain a protein concentrated solution; and (4) drying the protein concentrated solution to obtain RuBisCO protein powder. The plant leaves are at least one of green leaves such as medicago sativa and spinach. The mass ratio of the plant leaves to the water is 1: (1-49); the addition amount of the sodium pyrosulfite is 0.2%-2% of the total mass of the plant leaves and the water; the addition amount of the cross-linked polyvinylpyrrolidone is 0.5%-3% of the total mass of the plant leaves and the water. The extraction method provided by the invention can achieve the effects of high protein content and recovery rate, simple operation process and cost saving.