3 results about "Electron micrographs" patented technology

The present invention concerns the development of vesicles that could be used for generation of vaccines or as compound delivery vehicles. More specifically, the invention relates to a method for preparing a vesicle comprising the steps of: providing recombinant Trypanosoma brucei cells expressing sortaggable VSG, treating said cells in hypotonic solution in the presence of at least one protease inhibitor until the cells are lysed, isolating the cellular membranes from the solution, suspending the isolated membranes previously obtained in a isotonic solution, treating the suspended cellular membranes obtained in the previous step with sonication in order to obtain a vesicle suspension, removing aggregated membranous debris from the vesicle suspension previously obtained, separating the vesicle suspension into populations of vesicles, and providing vesicles from a population of vesicles which is characterized by the following parameters: (i) having a single predominant protein revealed after Coomassie staining an SDS PAGE that has an apparent molecular weight of 55 to 60 kDa, (ii) having a spherical appearance in electron micrographs and (iii) exhibiting a homogenous surface structure in electron micrographs. Moreover, the present invention also relates to a vesicle comprising sortaggable VSG characterized by the aforementioned parameters as well as such a vesicle for use in treating and / or preventing a disease or medical condition or as a compound delivery vesicle, preferably, drug delivery vehicle, more preferably, nucleic acid delivery vesicle. Finally, the invention contemplates a kit for carrying out the method of the present invention comprising recombinant Trypanosoma brucei cells expressing sortaggable VSG and at least one agent for carrying out the method of the present invention or a kit comprising the vesicle of the present invention.

A dispersion of fine inorganic particles comprising the following (a) to (c) components:(a) 10-70 mass % the inorganic fine particles, which are hydrophobized inorganic fine particles having a number-average primary-particle diameter, as determined by analyzing images on a transmission electron photomicrograph, of 10-200 nm;(b) 1.0-30 mass % aqueous component having one or more alcoholic hydroxyl groups; and(c) 1.0-20 mass % polyglycerin-modified silicone. The (c) component has dissolved in the (b) component. The dispersion has excellent dispersibility in aqueous media and high dispersion stability.

The present disclosure relates to an automatic measurement method for electron micrograph images obtained in a semiconductor process. The method comprises: obtaining an electron micrograph image to be analyzed as a first image; obtaining a region selected by a user in the first image as a first region, and obtaining information of a manual measurement operation performed by the user in the first region; obtaining a value of a specified image feature of the first region, and selecting one from a plurality of preset region matching algorithms as a first algorithm based on the specified image feature value; obtaining a value of a specified edge feature related to the manual measurement operation information, and selecting one from a plurality of preset edge recognition algorithms as a second algorithm based on the specified edge feature value; and recognizing at least one second region from the first image by using the first algorithm, recognizing an edge to be measured in the second region by using the second algorithm, and performing automatic measurement by using the edge.