41 results about "Lateral ventricles" patented technology

The invention relates to the technical field of medical equipment, and discloses an intelligent lateral ventricle and abdominal cavity shunt tube which comprises an inner core drainage tube, the two ends of the inner core drainage tube are closed, the inner core drainage tube is divided into a ventricle section, a middle section and an abdominal cavity section, the ventricle section of the inner core drainage tube is arranged in the ventricle of a patient, and the middle section of the inner core drainage tube is connected with the abdominal cavity section. The abdominal cavity section of the inner core drainage tube is arranged in the abdominal cavity of a patient, and the pressure monitoring device is arranged on the middle section of the inner core drainage tube and used for monitoring the shunt volume of cerebrospinal fluid. The technical means that the pressure monitoring device and the flow adjusting device are matched is adopted, the pressure in the ventricle is monitored in real time through the pressure monitoring device, the flow dividing amount of cerebrospinal fluid is adjusted in cooperation with the flow adjusting device, the defects in the prior art are overcome, and then the practicability of the device is improved.

The present invention discloses a Chinese medicine composition for preventing and / or treating Alzheimer's disease, wherein the raw materials of the Chinese medicine composition include Zanthoxylum bungeanum and Rehmannia glutinosa. The preparation method of the Chinese medicine composition for treating Alzheimer's disease provided by the present invention adopts easy-to-obtain raw materials, is simple to operate, has stable technology, can maximize the efficacy of the raw material components, and the prepared pharmaceutical composition has good effect and great commercial value; the Chinese medicine compound composition provided by the present invention can improve the lateral ventricular injection of Aβ 25‑35 As well as the spatial and non-spatial learning and memory abilities of AD dementia mice caused by the intraperitoneal injection model of scopolamine.

The application belongs to the field of neuropharmacology and medicine, and particularly relates to application of taurine cholic acid in preparation of a drug for preventing or treating Alzheimer's disease. Through animal model experiment verification, the A beta lateral ventricle injection is used to prepare an AD mouse model, and after oral administration of TCA for 2 weeks, cognitive behavior and pathological indexes are significantly improved.

The invention relates to a touch fluid neuron specific tracing imaging agent, a preparation method and application, and belongs to the technical field of nuclear medicine imaging, the tracing imaging agent is composed of a nerve tracer agent horseradish peroxidase (HRP), zirconium-89 isotope chelated by deferoxamine and a pharmaceutically acceptable carrier, the effective tracking window of the tracer agent is prolonged to 120 hours, and the effective tracking window of the tracer agent is prolonged to 120 hours. The multi-stage synaptic transfer period of the fluid touching neuron can be completely covered; the spatial resolution is improved by 5-8 times compared with that of traditional FDG-PET, and a fluid touching neuron cluster can be accurately presented; the in-vitro serum stability is excellent, the radiochemical purity is still larger than or equal to 80.5% within 120 hours, PET / MRI bimodal imaging can be adapted, drug administration is conducted through lateral ventricle minimally invasive injection, the probe is suitable for various animal models such as mice, rats and non-human primates, in-vivo dynamic tracing of sympathetic fluid neuron trans-synapses can be achieved on the premise that no tissue damage exists, and the probe has a good application prospect. And a key technical tool is provided for neural circuit analysis, neuroscience basic research and preclinical research of neurodegenerative diseases.

The invention discloses a modeling method for inducing cognitive impairment of mice through METH and HIV-1Tat protein, and relates to the technical field of animal experiment model construction.The method is characterized by comprising the steps that 10 micrograms / kg of HIV-1Tat protein is injected into the lateral ventricle of each mouse in a stereotactic mode, and then 2 mg / kg of METH solution is injected into the abdominal cavity of each mouse continuously for 7 days. The modeling method can simulate cognitive impairment under the combined action of METH and HIV-1Tat protein, an effective experimental model is provided for studying neurotoxicity synergistically induced by METH and HIV-1Tat protein, the mechanism of inducing mouse cognitive impairment by METH and HIV-1Tat protein can be further verified through the experimental model, and the method has the advantages of being high in application value and promising in application prospect. The development of potential treatment strategies and intervention measures is assisted.

The invention discloses a lateral ventricle drainage device capable of automatically adjusting pressure, which comprises a drainage tube capable of being mounted on a sliding block of a height adjusting frame, the height adjusting frame is existing and is equipped in hospitals, the sliding block is arranged in the middle of the height adjusting frame, scale marks are arranged on two sides of the height adjusting frame, and the sliding block can slide up and down to adjust the height. The pulley mechanism is installed on the sliding block and can generate elastic torsion when rotating, the pulley mechanism has the elastic torsion, the drainage tube bypasses the pulley mechanism from the upper side and then is divided into a right section and a left section, the right section is provided with an elastic expansion bag, and the left section is provided with a transparent dropper which is communicated with outside air and used for observing the dripping speed. The pulley mechanism has a clockwise torsion force to balance the gravity of the expansion bag, the tail end of the left section is connected with a drainage bag, when the drainage amount is increased, the expansion bag expands to drive the pulley mechanism to rotate clockwise, the dropper ascends to raise the position of the liquid outlet so as to reduce the drainage flow speed, and the flow speed adjustment is achieved.

The invention belongs to the technical field of biology, and particularly relates to a construction method of a respiratory syncytial virus infected mouse model. Disodium clodronate liposome with the concentration of 4ng / mL is injected into the lateral ventricle of the mouse through stereotactic positioning of the brain. 48h after injection, mouse brain tissues are taken to detect the activation state of microglial cells through immunofluorescence and WB, after it is confirmed that the microglial cells are inhibited, RSV (virus titer: 2.8 * 10 < 6 > PFU) nasal drop infection experiments are carried out on the mouse, and after the 5th day after virus infection, the mouse is infected by the RSV (virus titer: 2.8 * 10 < 6 > PFU) nasal drop infection experiments. Likewise, mouse brain tissue is taken for virus load detection, and neuropathological changes of the mouse brain tissue are observed through HE staining, immunofluorescence and TUNEL staining, so that the method can be used for constructing a research model of RSV-related nervous system diseases.

The invention discloses a dynamic positioning medical drainage bag height intelligent adjusting system, which comprises a height adjusting bracket, a drainage bag hook, a detection device, a control box and a controller, and is characterized in that the height adjusting bracket comprises a first servo guide rod and a second servo guide rod; the first servo guide rod and the second servo guide rod are both installed on the top of the control box, the first servo guide rod and the second servo guide rod are opposite in direction, the drainage bag hook is installed on the first servo guide rod, the weighing sensor and the drainage tube fixing device are installed on the drainage bag hook, and the drainage tube fixing device is installed on the drainage bag hook. The detection device is mounted on the second servo guide rod; the controller is arranged in the control box, and the controller is provided with a zero position detection module and a height adjusting module; the zero position detection module is used for determining a zero position parallel to the forehead or the lateral ventricle of a patient, and the height adjustment module is used for automatically adjusting the height of a drainage bag hook according to the zero position.

The application provides a brain cortex thickness morphological analysis method and system of MR images, and relates to the technical field of medical image data processing.The method comprises the following steps: obtaining a head three-dimensional high-resolution magnetic resonance T1 weighted image sequence of a patient with intractable epilepsy to be evaluated; performing bias field correction and brain tissue stripping on the image sequence to construct an initial brain parenchyma three-dimensional voxel model containing individual lateral ventricle expansion morphological characteristics; based on the initial brain parenchyma three-dimensional voxel model, extracting an initial isosurface of the interface between gray matter and white matter; calculating a local curvature tensor for a deep brain sulcus region in the initial isosurface; performing topological defect repair and mesh smoothing on the initial isosurface according to the local curvature tensor to obtain an individualized cortex surface mesh model eliminating ventricle expansion deformation artifacts.The application realizes accurate registration of image space and surgical physical space.

The invention discloses a fetal MRI (Magnetic Resonance Imaging) brain tissue segmentation method, system and device and a medium, and belongs to the segmentation of brain tissue images in the technical field of artificial intelligence. The method aims at solving the problems that in the prior art, a segmentation method based on deep learning cannot be suitable for manual measurement of multi-resolution data and lateral ventricle width, and measurement efficiency is low due to single-piece measurement. Comprising a U-Net segmentation module and a Resnet classification module, the U-Net segmentation module comprises a DWMF block and a decoder block, and the DWMF block and the decoder block are connected through a jump link block; the DWMF block comprises two depth separation convolution blocks and four depth separation channel expansion convolution blocks, and the output of one depth separation convolution block is used as the input of the last depth separation channel expansion convolution block after passing through three depth separation channel expansion convolution blocks.

The present application provides a compound for treating Alzheimer's disease designed based on artificial intelligence, a preparation method and medical uses thereof. The compound is shown as formula I. Through pharmacological experiments, it is found that compounds 548 and 398 both have the effect of inhibiting LPS-induced neuroinflammation, in addition, compound 548 can improve LPS lateral ventricle injection mouse neuroinflammation, improve Aβ 1‑42 Lateral ventricle injection mouse neuroinflammatory injury, so the compound shown as formula I can be used for preparing a drug for treating Alzheimer's disease.

The present invention discloses a lateral ventricle drainage set with precise positioning, comprising a precise positioning puncture device and a drainage device. The precise positioning puncture device includes a main body arc-shaped bracket, a puncture bracket, a bracket handle, a power supply, a deflection pointer, an angle scale, a catheter fixing slide, a drainage tube puncture catheter, a puncture steel needle, a drainage tube, a laser head and a scalp puncture needle. By setting an arc-shaped bracket, a bracket pin, a puncture bracket, a deflection pointer, a lateral displacement rod and an angle scale, when aligning the puncture point, first draw a line and determine the puncture point on the patient's scalp to determine the sagittal plane of the patient and the reference plane corresponding to the precise positioning puncture device. Place the arc-shaped bracket on the patient's head, rotate the deflection pointer to an appropriate angle, and then adjust the drainage tube to an appropriate position so that the drainage tube is aligned with the puncture point, making the positioning more precise during puncture, improving the puncture efficiency, and ensuring the accuracy of the puncture direction and depth.

This application provides a method for measuring fetal ventricular ratio and an ultrasound imaging system. The method is applied to an ultrasound imaging system, which includes a processor. The processor acquires an ultrasound image of a cross-section of the fetal lateral ventricle; the processor determines the midline of the brain based on the ultrasound image of the cross-section of the lateral ventricle; the processor acquires a first farthest point on the lateral wall of the lateral ventricle in the ultrasound image that is farthest from the midline of the brain, and acquires the length of a first perpendicular line from the first farthest point to the midline of the brain; the processor acquires a second farthest point on the medial side of the skull in the ultrasound image that is farthest from the midline of the brain, and acquires the length of a second perpendicular line from the second farthest point to the midline of the brain; the processor determines the fetal ventricular ratio based on the lengths of the first and second perpendicular lines. This application improves the efficiency of doctors and increases the accuracy of the obtained ventricular ratio.

The invention belongs to the technical field of prenatal detection, and discloses application of a fetal side ventricle broadening karyotype-CNV-seq-WES stepped detection strategy in prenatal genetic counseling and clinical prognosis analysis, retrospective queue research design is adopted, 166 cases of FVM diagnosed by prenatal ultrasound are incorporated, and the detection strategy is applied to prenatal genetic counseling and clinical prognosis analysis. The system analyzes the relevance of FVM severity, anatomy types and chromosome abnormalities. The clinical application value of the genetic detection technology is evaluated by comparing genetic detection results and pregnancy outcomes of different subgroups. Through the analysis, more information is provided for the fetal medicine multidisciplinary team, so that the quasi parents are helped to obtain more comprehensive prenatal consultation and decision support.

The present invention provides a neural stem cell that simultaneously expresses the genes of Pdgfrβ and Prom1. The present invention also provides a method for preparing the above-mentioned neural stem cells, including using transgenic mice Pdgfrβ-Cre transgenic mice and Cag-Frt-Stop-Frt-Lox-Stop-Lox-tdTomato reporter gene mice, hybridizing to produce Pdgfrβ-Cre; Ai65D double heterozygous experimental mice; constructing pAAV-mP2-FLP-WPRE viruses; isolating Pdgfrβ-Cre; Ai65D double heterozygous transgenic mice lateral ventricle lateral wall neural stem cells, after in vitro culture and passage, adding pAAV-mP2-FLP-WPRE viruses and tamoxifen's active metabolite 4-OH-tamoxifen; obtaining neural stem cells that simultaneously express Pdgfrβ and Prom1 genes. The present invention found that Pdgfrβ and Prom1 double-positive neural stem cells have the function of repairing nerve damage.

The invention discloses a brain puncture trocar for puncture of neonatal hydrocephalus through a fonfonis ventricle under ultrasonic guidance, and relates to the technical field of brain puncture trocar, the brain puncture trocar comprises a connecting seat main body, one end of the connecting seat main body is provided with a long round splicing piece, one side of the long round splicing piece is connected with a connecting hard cannula, and the other side of the long round splicing piece is connected with a connecting sleeve. The brain puncture trocar comprises a hard puncture needle and a connecting hard cannula, a liquid guide soft cannula is installed in the connecting hard cannula, and hydrophobic scale stripes are sprayed to the outer side of the liquid guide soft cannula, and it is ensured that the position of the hard puncture needle can be monitored in real time through an ultrasonic device in the puncture process of the brain puncture trocar; meanwhile, the position of the hard puncture needle is subjected to naked eye tracking through light spots formed by the optical fiber of the hard puncture needle under the skin of a patient, so that the depth and position monitoring mode in the puncture process of the brain puncture trocar is effectively expanded, and it is ensured that medical staff can clearly judge the puncture depth and position of the head of the brain puncture trocar in real time; therefore, the operation convenience and the operation precision of the whole brain puncture trocar are effectively improved.

This invention discloses the application of the FAM26F gene in the diagnosis and treatment of depression, belonging to the interdisciplinary field of molecular biology and neuropsychiatry. This invention reveals for the first time that downregulation of the FAM26F gene expression disrupts glutamate homeostasis by affecting the expression of glutamate transporters and receptors, thereby leading to depressive-like behaviors. Based on this, this invention provides two core applications: first, the application of the FAM26F gene in the preparation of diagnostic products for depression, achieving objective diagnosis by detecting the expression level of this gene in samples such as peripheral blood; second, the application of the FAM26F gene in the preparation of therapeutic drugs for depression, wherein the drug uses a gene therapy vector that upregulates the expression of this gene as its active ingredient, and is administered via lateral ventricle injection or nasal nebulization, effectively reversing depressive-like behaviors in animal models. This invention provides a novel, precise diagnostic target and targeted treatment strategy for depression.

The invention discloses a magnetic resonance imaging method for measuring the volume fraction of periarterial space cerebrospinal fluid with heart rate activity. The method comprises the steps that brain magnetic resonance images are collected, and meanwhile the time sequence of pulse signals is collected; the brain magnetic resonance image corresponds to the pulse signal period, and a plurality of pieces of magnetic resonance image data at equal intervals in the pulse signal period are generated; segmenting a lateral ventricle region in the brain magnetic resonance image, and calculating the CSF average signal intensity of cerebrospinal fluid in the lateral ventricle region; calculating a peak value and a valley value of a magnetic resonance signal time sequence one by one pixel or in an interested area to obtain a signal fluctuation change magnetic resonance signal difference value; dividing the magnetic resonance signal difference value of the signal fluctuation change by the average signal intensity of the lateral ventricle cerebrospinal fluid CSF to obtain the volume fraction caPVS of the periarterial space cerebrospinal fluid. The perivascular space cerebrospinal fluid volume fraction calculated by the method can effectively reflect perivascular space participating in lymphatic-like circulation, and the influence of pathological perivascular space such as expanded perivascular space is eliminated.

The application relates to a kind of brain CT perfusion image full-automatic post-processing methods, comprising: reading CT perfusion image;The CT perfusion image is preprocessed, and the skull perfusion image with skull is obtained, and the brain tissue image containing lateral ventricle after removing skull;Obtain lateral ventricle segmentation result binary graph, comprising: using the skull perfusion image with skull, obtain the optimal image layer of the largest connected domain area in skull;In the brain tissue image, from the optimal image layer, find the first distance towards the direction of skull top, find the second distance towards the direction of skull bottom, obtain the image layer containing lateral ventricle;The image layer containing lateral ventricle is converted into feature image, and lateral ventricle segmentation is carried out on each layer feature image, and lateral ventricle segmentation result binary graph is obtained;Using the brain tissue image, arterial input function and perfusion parameter map are sequentially obtained;The perfusion parameter map is binarized, and the lesion area is obtained in combination with the lateral ventricle segmentation result binary graph.

The invention relates to the technical field of medical experimental animal models, in particular to a construction method and application of an animal epilepsy model. The construction method of the animal epilepsy model provided by the invention comprises the following steps: performing anesthesia treatment on a mouse; tranexamic acid is injected into the side ventricle of the anesthetized mouse in a brain stereotactic administration mode; and recording and evaluating the epileptic seizure grade of the mouse injected with the tranexamic acid by adopting a Racine grading standard. The animal epilepsy model constructed by the invention is high in epilepsy causing rate, simple in modeling process, easy to operate and repeat, and capable of better reflecting epilepsy caused by tranexamic acid, so that the animal epilepsy model can be used for researching tranexamic acid related epilepsy pathogenesis and targeted intervention measures.

The present invention discloses a method, system, device and medium for fetal MRI brain tissue segmentation, belonging to the segmentation of brain tissue images in the field of artificial intelligence technology. The purpose is to solve the problems in the prior art that the segmentation method based on deep learning cannot be applied to multi-resolution data, and the manual measurement of the width of the lateral ventricle and single-shot measurement result in low measurement efficiency. It includes obtaining sample data and labels, constructing and training a lateral ventricle segmentation and classification model, and real-time segmentation and classification. The lateral ventricle segmentation and classification model includes a U-Net segmentation module and a Resnet classification module. The U-Net segmentation module includes a DWMF block and a decoder block, and the DWMF block and the decoder block are connected through a skip connection block; the DWMF block includes two depthwise separable convolution blocks and four depthwise separable channel dilated convolution blocks, and the output of one depthwise separable convolution block is used as the input of the last depthwise separable channel dilated convolution block after passing through three depthwise separable channel dilated convolution blocks.

The application belongs to the field of image segmentation, and relates to a lateral ventricle choroid plexus segmentation and quantification method based on enhanced T1 weighted magnetic resonance images, S1: obtaining brain enhanced T1 weighted magnetic resonance images and performing pretreatment to obtain standardized brain images; S2: based on the standardized brain images, combining a preset enhancement intensity threshold and a lateral ventricle anatomical position constraint, constructing a choroid plexus candidate region; S3: using a trained choroid plexus segmentation model to perform feature discrimination and segmentation on the choroid plexus candidate region to generate an initial segmentation mask; S4: performing post-processing optimization on the initial segmentation mask to remove false positive regions to obtain a final lateral ventricle choroid plexus segmentation mask; and S5: based on the final lateral ventricle choroid plexus segmentation mask, performing choroid plexus quantification parameter calculation. The problems of low segmentation precision, poor integrity and insufficient stability are solved.

The present invention discloses a teaching model and demonstration method for dynamically demonstrating the cerebrospinal fluid circulation pathway, relating to the field of demonstration models. The model comprises a brain shell having cavities including the lateral ventricles, the third ventricle, and the fourth ventricle, and the subarachnoid space; aqueducts are provided between the cavities and between the fourth cavity and the subarachnoid space; the interior of each cavity is connected to a water tank via a water inlet pipe and a water pump, and the water tank contains water simulating cerebrospinal fluid; a plurality of water outlets are provided on the subarachnoid space, and the water outlets are connected to the water tank via a drain pipe; elastic filler serving as brain tissue is provided between all cavities and between the cavity and the subarachnoid space; the outlet of each aqueduct is configured as a variable diameter section, and a float is placed in the aqueduct; a motor is provided outside each aqueduct, and the output shaft of the motor passes through the aqueduct and is connected to the float via a traction rope. The present invention enhances teaching effectiveness by dynamically simulating the normal circulation process of cerebrospinal fluid and the pathological and physiological changes after obstruction.

The invention discloses a neurological serious high-simulation invasive operation simulation training and neuroanatomy demonstration teaching system, and belongs to the technical field of medical education and clinical skill training. The system comprises a neuroanatomy demonstration system, a high-simulation invasive operation simulation training system and an integrated computer host. The dissection demonstration system visually displays key structures such as cerebral artery, vein, cranial nerve and cerebrospinal fluid circulation through a dynamic model and a three-dimensional visualization technology; the operation simulation training system adopts a biomimetic material human body model to truly simulate the hand feeling and feedback of more than ten neurological serious invasive operations such as lateral ventricle puncture, trachea cannula and central venous catheterization; the computer host integrates the functions of case scene simulation, process video recording and redisk and intelligent examination and evaluation. According to the invention, integration, scenario and intelligence of anatomy teaching and operation training are realized, a safe, repeatable and evaluable high-quality training environment is provided for students, and the efficiency and clinical practice ability of neurological intensive medicine teaching are effectively improved.

The present application belongs to the field of brain image processing, and particularly relates to a lateral ventricle widening prediction method, device, program product and medium, the steps of the method comprising: performing three-dimensional segmentation processing on a magnetic resonance image through a three-dimensional multi-sequence segmentation model to obtain a three-dimensional segmentation result; the three-dimensional multi-sequence segmentation model is constructed based on a three-dimensional nnUNet network, and a topological loss function is used to train the three-dimensional multi-sequence segmentation model; inputting the three-dimensional segmentation result into a prediction model to obtain a classification result of lateral ventricle adverse prognosis. The present application uses an nnUNet network to perform three-dimensional full-automatic segmentation of the lateral ventricle, and a topological loss (TopoLoss) is used in the segmentation process, and then a classification result of lateral ventricle adverse prognosis is obtained, which improves the continuity and accuracy of three-dimensional segmentation, and solves the problem that traditional two-dimensional segmentation cannot capture the overall anatomical morphology of the lateral ventricle.

The invention belongs to the field of brain image processing, and particularly relates to a lateral ventricle widening prediction method and device, a program product and a medium, and the method comprises the steps: carrying out the three-dimensional segmentation of a magnetic resonance image through a three-dimensional multi-sequence segmentation model, and obtaining a three-dimensional segmentation result; the three-dimensional multi-sequence segmentation model is constructed based on a three-dimensional nnUNet network, and a topology loss function is adopted to train the three-dimensional multi-sequence segmentation model; and inputting the three-dimensional segmentation result into a prediction model to obtain a classification result of lateral ventricle malfunction prognosis. According to the method, three-dimensional full-automatic segmentation of the lateral ventricle is carried out by adopting the nnUNet network, TopoLoss is adopted in the segmentation process, then a classification result of poor prognosis of the lateral ventricle is obtained, the continuity and accuracy of three-dimensional segmentation are improved, and the problem that the overall anatomical form of the lateral ventricle cannot be captured through traditional two-dimensional segmentation is solved.

The utility model relates to the field of medical instruments, in particular to a lateral ventricle drainage device which comprises an oval extrusion device, an empty flow blocking structure, a suspension bracket, a sleeve, a drainage bottle fixing device, a drainage bottle, a drainage tube, a suspension bracket base and a drainage monitoring structure. The sleeve is fixed on the suspension bracket through a first nut; the height of the drainage bottle is limited between 10 cm and 15 cm through the slidable range of the drainage bottle fixing device, it is guaranteed that the drainage bottle is in a safe range when used, the light generator and the receiver are arranged on the two sides of the drainage tube, the state in the drainage tube is detected, medical workers are timely alarmed and informed to check and operate, and the safety of the medical workers is guaranteed. The accelerated rotation of the elliptical knob is controlled to be in a clamping state through the PLC, it is ensured that air cannot enter the encephalic, the drainage speed is detected and the intracranial drainage speed is monitored through the light generator and the receiver, and the drainage speed can be controlled by controlling the rotation of the elliptical knob through the controller.

In the present invention, inventors demonstrate that prenatal excess of anti-Müllerian hormone triggers PCOS-like impairment in female sexual behavior in mice. Sexual dysfunction in PCOS-like mice is associated with decreased expression of neuronal nitric oxide synthase (nNOS) neurons in different hypothalamic regions known to be involved in female sexual behavior: rostral periventricular area of the third ventricle (RP3V), ventromedial nucleus of the hypothalamus (VMH), and arcuate nucleus (ARN) during estrus. Chemogenetic inhibition of nNOS neuronal activity in the ventromedial nucleus of the hypothalamus of control adult females recapitulates PCOS-like sexual dysfunction. Of clinical relevance, administration of nitric oxide (NO) donor rescues normal sexual behavior in PCOS-like mice. Accordingly, the present invention relates to invention relates to Nitric Oxyde (NO) agent for use in the prevention or the treatment of sexual dysfunction associated with Polycystic Ovary Syndrome (PCOS) or with Hypoactive Sexual Desire Disorder (HSDD) in a subject in need thereof.

The application discloses a gene medicine for treating deafness-dystonia-optic neuropathy syndrome caused by TIMM8A gene defect and application. The medicine core is a modularly designed adeno-associated virus vector system which carries an expression cassette containing a tissue-specific promoter and a human TIMM8A treatment gene. The application realizes precise treatment through local minimally invasive injection according to different symptoms: AAV-Anc80L65-MYO15-TIMM8A is injected into the cochlea to treat sensorineural hearing loss; AAV2 / 4-Brn3b-TIMM8A is injected into the vitreous body of the eyeball to treat optic neuropathy; AAV9-hSyn-TIMM8A is injected into the central lateral ventricle to treat dystonia. Preclinical studies have confirmed that the strategy can efficiently express TIMM8A protein in the corresponding target cells, significantly repair hearing, visual and motor function defects, and correct mitochondrial dysfunction from the pathological mechanism.

The invention discloses a teaching model and a demonstration method for dynamically demonstrating a cerebrospinal fluid circulation pathway, and relates to the field of demonstration models, and the teaching model comprises a cavity with a lateral ventricle, a third ventricle and a fourth ventricle, and a brain shell of a subarachnoid space; water guide pipes are arranged between the cavities and between the fourth cavity and the subarachnoid cavity, the interior of each cavity is communicated with a water tank through a water inlet pipe and a water pump, and water simulating cerebrospinal fluid is stored in the water tank; a plurality of water outlets are formed in the subarachnoid cavity and are communicated with the water tank through drainage pipes; elastic filling materials serving as brain tissues are arranged among all the cavities and between the cavities and the subarachnoid cavity; an outlet of each water guide pipe is arranged to be a variable-diameter section, and a floating ball is placed in each water guide pipe. A motor is arranged outside each water guide pipe, and an output shaft of each motor penetrates through the corresponding water guide pipe to be connected with the corresponding floating ball through a traction rope. According to the invention, the teaching effect is enhanced by dynamically simulating the normal circulation process of cerebrospinal fluid and the pathophysiological change after obstruction.