Method for studying migration, proliferation and differentiation of brain endogenous neural stem cells

A neural stem cell, endogenous technology, applied in the field of basic medicine, can solve obvious side effects, can not delay the progress of the disease, movement disorders and other problems

Inactive Publication Date: 2014-07-23
CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At present, classic drug treatment such as oral levodopa (L-dopa) can reduce or relieve symptoms in the early stage, but it cannot delay the progress of the disease, and long-term

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  • Method for studying migration, proliferation and differentiation of brain endogenous neural stem cells
  • Method for studying migration, proliferation and differentiation of brain endogenous neural stem cells
  • Method for studying migration, proliferation and differentiation of brain endogenous neural stem cells

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Embodiment Construction

[0023] Here, some English abbreviations involved in the description of the present invention are explained, and the English abbreviations mentioned in the following text are all understood according to this explanation:

[0024] PD (Parkinson's disease) Parkinson's disease;

[0025] AD (Alzheimer disease) Alzheimer's disease;

[0026] DA (dopamine) dopamine;

[0027] L-DOPA (levodopa) levodopa;

[0028] PB (phosphate buffer) phosphate buffer;

[0029] PBS (phosphate buffered saline) phosphate buffered saline;

[0030] SN (Substantia nigra) substantia nigra;

[0031] TH (tyrosine hydroxylase) tyrosine hydroxylase;

[0032] 6-OHDA (6-Hydroxydopamine) 6-hydroxydopa;

[0033] Endo-N (endoneuraminidase) PSA-specific lyase;

[0034] EGF (epidermal growth factor) epidermal growth factor;

[0035] BDNF (brain derived neurotrophin factor) brain-derived neurotrophic factor;

[0036] GFAP (glial fibrillary acidic protein) glial-derived fibrillary acidic protein;

[0037] SGZ (s...

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Abstract

The invention relates to a method for studying migration, proliferation and differentiation of brain endogenous neural stem cells. The method comprises the following steps: (1) performing double-labeled immunofluorescent staining on an animal brain section by 5-bromndeoxyuridine and doublecortin, 5-bromndeoxyuridine and neuronal nuclei antigen as well as 5-bromndeoxyuridine and glial cell line-derived neurotrophic factor, and single-labeled immunofluorescent staining by sialyl neural cell adhesion molecules; and (2) carrying out statistic analysis on data and drawing by GraphPad Prism6.0 software, and performing one-way variance analysis on single-factor multi-group data of the 5-bromndeoxyuridine positive cells, wherein the animal brain section is subjected to the following treatment: stereotactically injecting a 6-hydroxydopamine solution into a corpus striatum of the right side of the brain, injecting a PSA (prostate-specific antigen) specificity lyase stock solution into the lateral ventricle, and then injecting a 5-bromndeoxyuridine solution in the abdominal cavity. An effective method for studying migration influence of the brain endogenous neural stem cells is provided.

Description

technical field [0001] The invention belongs to the field of basic medicine, and in particular the invention relates to a method for studying the effects of migration, proliferation and differentiation of neural stem cells in the brain. Background technique [0002] Parkinson's disease (PD) is a common progressive degenerative disease of the central nervous system characterized by lesions in the nigrostriatal pathway. At present, the pathogenesis of PD is not yet clear, and it is the result of the synergistic effect of multiple mechanisms. Clinical therapy cannot fundamentally prevent the progressive degeneration of the nigrostriatal pathway in PD patients. Recently, more and more people believe that the activation of brain endogenous neural stem cells to participate in brain injury repair is likely to develop into a new Strategies for treating brain injuries. This therapeutic strategy is particularly applicable to the repair of the chronic brain damage disease - Parkinson...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N33/533
CPCG01N33/5058G01N33/5073G01N33/582G01N2800/2835
Inventor 段维明张永鑫杨春
Owner CAPITAL UNIVERSITY OF MEDICAL SCIENCES
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