Method for studying migration, proliferation and differentiation of brain endogenous neural stem cells

Abstract

The invention relates to a method for studying migration, proliferation and differentiation of brain endogenous neural stem cells. The method comprises the following steps: (1) performing double-labeled immunofluorescent staining on an animal brain section by 5-bromndeoxyuridine and doublecortin, 5-bromndeoxyuridine and neuronal nuclei antigen as well as 5-bromndeoxyuridine and glial cell line-derived neurotrophic factor, and single-labeled immunofluorescent staining by sialyl neural cell adhesion molecules; and (2) carrying out statistic analysis on data and drawing by GraphPad Prism6.0 software, and performing one-way variance analysis on single-factor multi-group data of the 5-bromndeoxyuridine positive cells, wherein the animal brain section is subjected to the following treatment: stereotactically injecting a 6-hydroxydopamine solution into a corpus striatum of the right side of the brain, injecting a PSA (prostate-specific antigen) specificity lyase stock solution into the lateral ventricle, and then injecting a 5-bromndeoxyuridine solution in the abdominal cavity. An effective method for studying migration influence of the brain endogenous neural stem cells is provided.

Description

technical field [0001] The invention belongs to the field of basic medicine, and in particular the invention relates to a method for studying the effects of migration, proliferation and differentiation of neural stem cells in the brain. Background technique [0002] Parkinson's disease (PD) is a common progressive degenerative disease of the central nervous system characterized by lesions in the nigrostriatal pathway. At present, the pathogenesis of PD is not yet clear, and it is the result of the synergistic effect of multiple mechanisms. Clinical therapy cannot fundamentally prevent the progressive degeneration of the nigrostriatal pathway in PD patients. Recently, more and more people believe that the activation of brain endogenous neural stem cells to participate in brain injury repair is likely to develop into a new Strategies for treating brain injuries. This therapeutic strategy is particularly applicable to the repair of the chronic brain damage disease - Parkinson...

AI Technical Summary

Problems solved by technology

[0003] At present, classic drug treatment such as oral levodopa (L-dopa) can reduce or relieve symptoms in the early stage, but it cannot delay the progress of the disease, and long-term use has obvious side effects, namely L-dopa-induced dyskinesia

Surgical procedures such as deep brain stimulation of the hypothalamic nucleus can only temporarily reduce or relieve symptoms

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