Evaluation model for rat neurotoxicity of parotitis virus

A mumps virus and neurovirulence technology, applied in the direction of instruments, biological systems, analytical materials, etc., can solve problems such as unsatisfactory, time-consuming slice processing, complicated operation, etc.
CN110736654AActive Publication Date: 2020-01-31SHANGHAI KING CELL BIOTECHNOLOGY CO LTD +1
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHANGHAI KING CELL BIOTECHNOLOGY CO LTD
Publication Date
2020-01-31

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Abstract

The invention provides an evaluation model for rat neurotoxicity of parotitis virus, concretely a device for evaluating the neurotoxicity of the parotitis virus. The model comprises(I) a virus inoculation module for performing virus inoculation of the parotitis virus to be evaluated to the lateral ventricle of a rat; (II) a processing module for carrying out vibration slicing on the fixed rat brain; (III) an imaging module for scanning and imaging an obtained mouse brain slice; and (IV) an analysis module used for calculating a neurotoxicity index through a formula I: the neurotoxicity index =S1 / S0 *100 (formula I) according to the cross sectional area S1 of a cavity formed by hydrocephalus in the longitudinal section of the rat brain and the total cross sectional area S0 of the rat brainin the obtained image. The results show that the result is stable, the stability is high, wild strains can be distinguished from vaccine strains, and compared with an existing monkey body neurotoxicity model, the animal cost and the operation difficulty are greatly reduced.
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Description

technical field

[0001] The invention belongs to the technical field of animal model construction, and more specifically relates to a rat neurotoxicity evaluation model of mumps virus. Background technique

[0002] Mumps virus is an RNA virus belonging to the Paramyxoviridae family, which can spread through droplets and invade the parotid gland and other glandular organs of children such as testis, ovary, pancreas, kidney and central nervous system. The clinical manifestations of the patient are swelling of one or both parotid glands, accompanied by fever, fatigue, muscle pain, etc., accompanied by a certain probability of complications of orchitis and encephalitis. The mumps vaccine strains currently on the market are all attenuated strains obtained from isolated wild strains through passage and attenuation.

[0003] In view of the neurotoxicity of mumps virus, neurotoxicity evaluation of mumps vaccine is an important indicator of vaccine safety. At present, the neurotoxic...

Claims

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