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Evaluation model for rat neurotoxicity of parotitis virus

A mumps virus and neurovirulence technology, applied in the direction of instruments, biological systems, analytical materials, etc., can solve problems such as unsatisfactory, time-consuming slice processing, complicated operation, etc.

Active Publication Date: 2020-01-31
SHANGHAI KING CELL BIOTECHNOLOGY CO LTD +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, in the above-mentioned rat neurotoxicity model, the processing of the slices takes a long time and the operation is complicated. More importantly, the natural shape of the brain slices is changed by the multiple dehydration, clearing, washing and other processes of HE staining, resulting in abnormalities in the cross-section of the brain. changes, so that the statistical analysis of the results is artificially disturbed, and the current slice processing method is still unsatisfactory

Method used

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  • Evaluation model for rat neurotoxicity of parotitis virus
  • Evaluation model for rat neurotoxicity of parotitis virus
  • Evaluation model for rat neurotoxicity of parotitis virus

Examples

Experimental program
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Embodiment 1

[0067] Example 1: Establishment of a rat model for evaluation of mumps virus neurovirulence

[0068] 1.1 Animal and virus dilution

[0069] Four pregnant Wistar rats of SPF grade were selected and randomly divided into four groups, namely A, B, C and D (the number of suckling rats in each group was not less than 10). Among them, group A was inoculated with DMEM medium as the control group, group B was inoculated with the vaccine strain, group C was inoculated with wild mumps strain passaged by Vero cells for 10 times, and group D was inoculated with wild mumps strain. B, C, D 3 groups are 4.0lg CCID with DMEM culture medium dilution virus titer 50 / mL.

[0070] 1.2 Virus inoculation

[0071] After the birth of the mother mouse, fix the 1-day-old suckling mouse, draw 10 μL of the virus liquid with a sterilized 20 μL micro-syringe (specification 27G), and inject it at the middle of the anterior blotch and herringbone point of the brain of the suckling mouse, on the left side ...

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Abstract

The invention provides an evaluation model for rat neurotoxicity of parotitis virus, concretely a device for evaluating the neurotoxicity of the parotitis virus. The model comprises(I) a virus inoculation module for performing virus inoculation of the parotitis virus to be evaluated to the lateral ventricle of a rat; (II) a processing module for carrying out vibration slicing on the fixed rat brain; (III) an imaging module for scanning and imaging an obtained mouse brain slice; and (IV) an analysis module used for calculating a neurotoxicity index through a formula I: the neurotoxicity index =S1 / S0 *100 (formula I) according to the cross sectional area S1 of a cavity formed by hydrocephalus in the longitudinal section of the rat brain and the total cross sectional area S0 of the rat brainin the obtained image. The results show that the result is stable, the stability is high, wild strains can be distinguished from vaccine strains, and compared with an existing monkey body neurotoxicity model, the animal cost and the operation difficulty are greatly reduced.

Description

technical field [0001] The invention belongs to the technical field of animal model construction, and more specifically relates to a rat neurotoxicity evaluation model of mumps virus. Background technique [0002] Mumps virus is an RNA virus belonging to the Paramyxoviridae family, which can spread through droplets and invade the parotid gland and other glandular organs of children such as testis, ovary, pancreas, kidney and central nervous system. The clinical manifestations of the patient are swelling of one or both parotid glands, accompanied by fever, fatigue, muscle pain, etc., accompanied by a certain probability of complications of orchitis and encephalitis. The mumps vaccine strains currently on the market are all attenuated strains obtained from isolated wild strains through passage and attenuation. [0003] In view of the neurotoxicity of mumps virus, neurotoxicity evaluation of mumps vaccine is an important indicator of vaccine safety. At present, the neurotoxic...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N1/28G01N21/84G01B11/28G01N1/36G01N1/44G16B5/00
CPCG01N1/30G01N1/286G01N21/84G01B11/28G01N1/36G01N1/44G16B5/00G01N2001/2873G01N2001/305G01N2001/366G01N2001/302C12N7/00G06T7/0012C12N2760/18711G01N33/569
Inventor 田大勇阮俊程傅振芳张亚静顾玉林安祺
Owner SHANGHAI KING CELL BIOTECHNOLOGY CO LTD
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