38 results about "Schizandrol A" patented technology

The invention relates to a Chinese magnoliavine fruit monomer composition separation preparation method; ethanol extracts from Chinese magnoliavine fruit are extracted by petroleum ether, chromatography is carried out to the petroleum ether extracts by a silicagel column, and then the petroleum ether is eluted by petroleum ether-ethylacetate, and HPLC is used for monitoring, and then crude extract A and crude extract B are fraction-collected, and eluted; the volume ratio of eluant petroleum ether and ethylacetate is 3-5:1, the crude extract A and crude extract B are respectively applied to a high-speed counter-current chromatography for separation, so as to obtain SCHisanhenol, deoxyschizandrin, schizandrin B, schisandrin C, schizandrol A, schizandrol B and schisantherrin B monomers, and the purity is higher than 98 percent.

The invention relates to a schisandra fruit fingerprint spectrum quality control method, comprising that (1), adding 0.5g schisandra fruit to extract via microwave for 50min, filtering, (2), washing flow phase gradient that the spectrum column is Eclipse, as 0-40min, 60-20% A, 40-80% B, flow speed is 0.8-1.2ml/min, the wavelength is 210-280nm, the column temperature is 20-40Deg. C, and the sample amount is 10-20ul, (3), building standard fingerprint spectrum that the first peak is schisandra fruit alcohol 1, the third peak is schisandra fruit alcohol 2, the fourth peak is gemixin N/ isomer, the sixth peak is schisandra fruit I element, the seventh and eighth peaks are gemixin N/ isomer, the ninth peak is schisandra fruit II element, (4), controlling the quality of fingerprint spectrum that the check peak relative holding times are 1.00, 1.25, 1.63, 1.88, 3.18 and 3.27, (5), the schisandra fruit planting collecting method. The invention can control the quality of materials to assure the stable quality of product.

The invention discloses a pharmaceutical composition for ventilating lung and relieving asthma, a preparation method and a quality control method. The pharmaceutical composition comprises the following pharmaceutical raw materials: ephedra herb, raw ginger, cassia twig, bitter apricot seed, Chinese magnoliavine fruit (prepared) and prepared liquoric root, the preparation method is as follows: the five herbal medicines except the bitter apricot seed are decocted by adding water through a countercurrent circulatory extraction process and concentrated to obtain a clear paste after reducing pressure, ethanol is added, then the mixture is stirred evenly and placed staticly, supernatant liquid is taken, the ethanol is recovered, and the concentration is carried out to obtain a thick paste; the bitter apricot seed is arranged in a hot pressure sterilization pot for hot pressure steaming and boiling and then is arranged in a small amount of cold water for soaking instantly after being taken out, an apricot processed product can be obtained by removing seed coats and being dried; the quality control method is as follows: the thin layer identification is carried out on the ephedra herb, the raw ginger and the liquoric root, the content measurement is carried out on ephedrine in the ephedra herb and schizandrin in the Chinese magnoliavine fruit. The pharmaceutical composition has good effects on ventilating lung and relieving asthma.

The invention relates to a quality detection method for a liver protection dropping pill of traditional Chinese medicine preparation. The quality detection method comprises the following steps: measuring the content of effective components of the liver protection dropping pill, including saikoside a and schizandrin, and identifying schisandra chinensis, pulvis fellis suis and artemisia capillaris in the liver protection dropping pill. The quality detection method also comprises the following detection steps: (1) with the saikoside a as a reference substance, measuring whether a radix bupleuri component is contained in a liver protection dropping pill recipe by adopting a high efficiency liquid chromatography method; (2) with the schizandrin as the reference substance, measuring whether a schizandrin component is contained in the liver protection dropping pill by adopting the high efficiency liquid chromatography method; (3) with the schisandrin b as the reference substance, identifying whether a schisandra chinensis component is contained in the liver protection dropping pill by adopting a thin-layer chromatography method; (4) with hyodeoxycholic acid as the reference substance, identifying whether a pulvis fellis suis component is contained in the liver protection dropping pill by adopting the thin-layer chromatography method; and (5) with chlorogenic acid as the reference substance, identifying whether an artemisia capillaris component is contained in the liver protection dropping pill by adopting the thin-layer chromatography method. The quality detection method disclosed by the invention can be used for effectively and reliably controlling the quality of the liver protection dropping pill, and the method is scientific, feasible and reliable.

The invention belongs to the field of natural organic chemistry, relating to a method for enriching and purifying schizandrol A in schisandra by utilizing macroporous adsorbent resin. The invention is characterized in that the macroporous adsorption technology, which is used for separating and purfying the schizandrol A, has good adsorption selectivity on the schizandrol A, rapid adsorption, rapid resolution and large adsorption capacity; and the extraction is convenient and fast, the source of raw materials is abundant, the production cost is low, the separating effect is obvious, the extraction purity is high, and the semi-finished product of the schizandrol A of which the content is more than 35% and the final product of the schizandrol A of which the content is more than 98% can be obtained, so that the defects that relatively low extraction rate and low extraction purity of routine extraction are overcomed. The macroporous absorbent resin selected by the invention has stable physicochemical property, larger surface area, fast exchange speed, high mechanical strength, strong anti-pollution ability and good thermostability, thereby being capable of selectively adsorbing the schizandrol A from solution by physical adsorption with the characteristics of fast adsorption, fast resolution and larger adsorption capacity.

The invention discloses a fingerprint spectrum detection method and a fingerprint spectrum of a Yixinshu preparation. The fingerprint spectrum detection method comprises the following steps: by taking a schizandrin reference substance as reference, measuring the fingerprint spectrum by adopting high performance liquid chromatography (HPLC). The similarity of the HPLC fingerprint spectrum of the Yixinshu preparation established in the method and a reference spectrum is more than 0.9, the quality of the fingerprint spectrum can be effectively characterized, and the product quality is comprehensively monitored. Moreover, a common mode of the HPLC characteristic fingerprint spectrum of the Yixinshu preparation is established, 15 common peaks are calibrated, the established fingerprint spectrum is high in technical content, the simplicity and one-sidedness of the quality control of the Yixinshu preparation are avoided, and the possibility that the quality of the artificially handled product reaches the standard is reduced.

The invention discloses a method for detecting a Chinese medicinal composition for freeing lung and relieving asthma. The Chinese medicinal composition consists of the following raw material medicines: ephedra, ginger, cassia twig, bitter apricot seed, Chinese magnoliavine fruit (prepared) and roasted liquorice. The detection method comprises the steps of detecting the ephedrine content of the ephedra and detecting the Schizandrol content of the Chinese magnoliavine fruit.

The invention discloses an extractive of fructus schisandrae and particularly relates to an ethanol extractive extracted from roots of fructus schisandrae and containing 15% of schizandrin or an ethanol extractive extracted from xylem of rhizome of fructus schisandrae and containing 18% of schizandrin. By means of the extractive, the problem of shortage in the fructus schisandrae raw material is solved, resources of the roots of the fructus schisandrae and the abandoned xylem of the rhizome of the fructus schisandrae in processing are made full use of, and waste is reduced. The extractive of fructus schisandrae is high in content of lignans such as schizandrin and different in content and composition of lignans compared with fruits and rattan of the fructus schisandrae and has unique activity in pharmacological activity. The invention further discloses the preparation method of the extractive of fructus schisandrae and the application of the extractive of fructus schisandrae in the aspect of hangover cure and liver protection.

Extraction of Schisandra chinensis alcohol-A is carried out by extracting Schisandra chinensis by solvent, extracting extract containing Schisandra chinensis alcohol-A, column chromatography separating, recovering elutropic solvent in eluent containing Schisandra chinensis alcohol-A and obtaining the final product concentrate. It is cheap and simple, has higher purity, recovery rate and quality.

The invention discloses a liver-protecting granule for poultry and a preparation method thereof. The granule is prepared from radix bupleuri, oriental wormwood, radix isatidis, schisandra chinensis, pulvis fellis suis and mung bean, and is modified from the liver-protecting tablet. The liver-protecting granule has the effects of soothing liver and regulating qi, strengthening spleen and promoting digestion, and are used for fatty liver syndrome of chicken; furthermore, the extraction process is improved, and the yield of schizandrin extracted from schisandra chinensis is relatively high; specific auxiliary dosage is adopted, so that the granule has high yield, excellent solubility and excellent granularity.

The invention provides application of schizandrin A in detection of cytochrome P4503A enzymatic activity and a detection method thereof. The schizandrin A can be used as probe substrate to detect the cytochrome P4503A enzymatic activity, and the schizandrin A or other medicines are used as specific probe substrate to obtain schizandrin to be used as metabolite at the reaction temperature between 10 DEG C and 60 DEG C. The decrement of the schizandrin A substrate or the metabolite generation amount of the schizandrin A is used as an evaluation index of cytochrome P4503A subfamily enzymatic activity. Or a mammal to be detected is enabled to take the schizandrin A or other medicines with 0.01-1000mg/kg of weight of the mammal through an intravenous injection, muscle injection or oral way; a time point is selected within 0-72h, and then a biological sample of blood plasma, urine, excrement or bile of the mammal to be detected is collected; and the decrement of the schizandrin A substrate or the metabolite generation amount of the schizandrin A is measured to be used as the evaluation index of the cytochrome P4503A subfamily enzymatic activity.

The invention discloses three Chinese magnoliavine fruit extracts as well as a preparation process and application thereof, and belongs to the technical field of medicines. The preparation method of the Chinese magnoliavine fruit extract comprises the following steps: treating Chinese magnoliavine fruit by using a CSFE method to obtain an extract A, and placing the extract at room temperature for layering to obtain an extract B (rich in schisandrin B) and an extract C (rich in schizandrin); boiling the decoction dregs, adding beta-cyclodextrin into filtrate to carry out inclusion adsorption on residual fat-soluble active ingredients in the decoction dregs, and filtering to obtain the inclusion adsorption lignan; performing concentration and alcohol precipitation on the filtrate to obtain the Chinese magnoliavine fruit polysaccharide; respectively combining the inclusion adsorption lignan and polysaccharide with the extracts A, B and C to obtain an extract I, an extract II and an extract III. The content of effective parts of the three extracts accounts for more than 50% of the extracts, the three extracts are composed of three active components including total lignan, linoleic acid and polysaccharide, and the total lignan is 18-25%. Different new drugs can be developed from the three extracts according to different proportions of schisandrin B and schizandrin for resisting depression, promoting sleep, promoting intelligence and other indications.

The invention provides a method for simultaneously determining psoralen, isopsoralen, dehydrodiisoeugenol, schizandrol A, evodiamine and rutaecarpine in a Sishen pill through a flash evaporation-gas chromatography technology. The method comprises the following steps: carrying out flash evaporation-gas chromatography detection on a Sishen pill sample to obtain a qualitative analysis result, carrying out an external standard technology to establish a standard working curve, and acquiring quantitative analysis results of the index components in the Sishen pill sample. The method adopting the flash evaporation-gas chromatography to realize the direct introduction of a solid sample without a complex extraction process or a solvent, so the method is simple to operate, and is environmentally-friendly; and the method adopting the flash evaporation-gas chromatography to quantitatively detect the index components in the Sishen pill can effectively save the experiment time and simplify experiment steps, so the above quality evaluation method has the characteristics of simplicity, environmental friendliness and high efficiency, and is of very far-reaching significance to evaluate the quality of the Sishen pill.

The invention relates to magnolia vine fruit oil with a human body heath care function, which is prepared by directly extracting plant magnolia vine fruits. The magnolia vine fruit oil mainly comprises the following effective components by weight percent: 0.5-0.75% of schizandrol A, 0.08-0.12% of tablet, capsule or oral liquid can be prepared bymagnolia vine fruit oil as the main active component and coordinating with pharmaceutically acceptable carriers or auxiliary materials, which has the health care functions of protecting liver, relaxing vessels, reducing blood fat and the like.

The invention especially relates to a determination method for lignan content, belonging to the technical field of content determination. The method provided by the invention has the advantages of high efficiency and simple and flexible operation. The method comprises the following steps: weighing and putting schisandrol A, schisandrol B and a schisandrol A reference substance in a same volumetric flask, adding methanol to a scale, carrying out shaking up and filtration and taking a subsequent filtrate as a mixed standard reference substance solution A; weighing and putting deoxyschizandrin, schisandrin B, a schisandrin C control product in a same volumetric flask, adding methanol to a scale, carrying out shaking up and filtration and taking a subsequent filtrate as a mixed standard reference substance solution B; weighing Chinese magnoliavine fruit, smashing and grinding the Chinese magnoliavine fruit, then carrying out sieving, weighing the obtained powder, adding 75% alcohol, weighing the weight, carrying out ultrasonic treatment for 10 to 60 min, taking an obtained substance out, cooling the substance to room temperature, adding 75% ethanol to compensate for lost weight, carrying out shaking up and filtration and taking a subsequent filtrate so as to obtain a to-be-determined sample solution; and respectively weighing the mixed standard reference substance solutions A and B, respectively diluting the solutions 1 time and respectively injecting the solutions into a liquid chromatograph.

The invention discloses identification and adulteration inspection methods for the component of the fruit of Chinese magnoliavine in eyesight-improving pills with the stem of noble dendrobium. According to the identification method, the eyesight-improving pill with the stem of noble dendrobium is identified by taking schizandrin as a reference substance through thin-layer chromatography. The adulteration inspection method comprises the following steps: detecting the contents of schizandrin and anwuligan in the eyesight-improving pill with the stem of noble dendrobium by adopting a high performance liquid chromatography-mass spectrometry, and then judging the authenticity of the stem of noble dendrobium in the eyesight-improving pill according to the ratio of the content of the anwuligan to the content of the schizandrin. By establishing a thin-layer chromatography identification method and a high performance liquid chromatography-mass spectrometry detection method for the stem of noble dendrobium in the eyesight-improving pill, the quality condition of the stem of noble dendrobium in the eyesight-improving pill is investigated, whether a production enterprise has the behavior of no feeding or adulterated feeding or not is checked, the method is simple, the cost is low, and the result is accurate. The medication safety of the eyesight-improving pill can be effectively ensured.