7 results about "Eleutheroside" patented technology

The present invention discloses a method for preparing a polysaccharide from Acanthopanax senticosus medicinal residue that reduces Aβ deposition in Caenorhabditis elegans. The method comprises the following steps: using waste generated in the elegans glycoside extraction process as a raw material, drying and crushing the waste, adding water and stirring evenly, subjecting the waste to dynamic high-pressure microfluidization homogenization, adding a surfactant to the treated liquid for ultrasonic extraction, centrifuging the obtained polysaccharide extract to obtain the supernatant, concentrating the supernatant under reduced pressure, and precipitating the polysaccharide from the Acanthopanax medicinal residue with alcohol. The present invention uses Acanthopanax medicinal residue as a raw material, recovers and reuses the active ingredients in the Acanthopanax medicinal residue, and promotes the sustainable development of the Acanthopanax medicinal residue industry. The preparation method disclosed in the present invention has the advantages of being easy to operate, time-saving, efficient, and pollution-free. The Acanthopanax medicinal residue polysaccharide prepared by the present invention has the efficacy of reducing Aβ deposition in Caenorhabditis elegans.

The invention discloses a traditional Chinese medicine beverage capable of soothing nerves, helping sleep and regulating emotion and a preparation method thereof, and particularly relates to the field of traditional Chinese medicine pharmaceutics. The beverage realizes sleep aiding through jujuboside, spinosin, eleutheroside, cyclic adenosine monophosphate and the like, regulates emotion through fingered citron lactone, rose volatile oil and the like, and tranquilizes and stabilizes mind by using hericium erinaceus, hawthorn flavone and the like. The preparation method comprises the following steps: carrying out differential pretreatment on raw materials; volatile oil, water-soluble functional components and flavor substances are obtained through circulating percolation extraction, dynamic countercurrent extraction and mild extraction by adopting a stepped directional extraction process; and precisely filtering and clarifying the extracting solution through a ceramic membrane, compounding with aromatic volatile oil, and sterilizing and filling by adopting a 550MPa ultrahigh-pressure cold sterilization technology. The method overcomes the defects of low extraction efficiency and easy loss of active components in the traditional process, realizes efficient synergistic extraction and complete retention of various active components, and has the advantages of fresh taste, stable character, long shelf life and accurate and controllable process parameters.

The invention discloses an acanthopanax sessiliflorus beverage and a preparation method and device thereof, and relates to the technical field of acanthopanax sessiliflorus beverage preparation, and the acanthopanax sessiliflorus beverage comprises an acanthopanax sessiliflorus whole plant mixed extract, a composite natural sweetener, a composite natural acidity regulator, a plant source stabilizer and deionized water. The acanthopanax sessiliflorus whole plant mixed extract is obtained by mixing roots, stems, leaves and fruits of acanthopanax sessiliflorus according to a weight ratio of 3: 2: 2: 3 and then carrying out a gradient extraction process, the total content of eleutheroside is greater than or equal to 6mg / 100ml, and the content of flavonoid compounds is greater than or equal to 4mg / 100ml. According to the acanthopanax sessiliflorus beverage and the preparation method and device thereof, the acanthopanax sessiliflorus whole plant-roots, stems, leaves and fruits are extracted according to a specific proportion, natural components of all parts are reserved, no extra essence needs to be added, and the flavor is more natural; the content of eleutheroside and flavonoid compounds in the whole plant mixed extract reaches the standard, the whole plant mixed extract has the effects of resisting fatigue and the like, and waste of root, stem and leaf resources is avoided.

The invention discloses a method for producing eleutheroside E and oleanolic acid by utilizing microbial fermentation, relates to the technical field of microbial fermentation, and aims to solve the problems that plant resources are damaged and the environment is polluted by the conventional method for obtaining the eleutheroside E and the oleanolic acid. The method comprises the following steps: 1, culturing activated pantoea agglomerans HASX4 to obtain a seed solution, carrying out enlarged culture, and collecting a fermentation solution; 2, eluting the fermentation liquor through a macroporous resin column, and collecting eluent; carrying out silica gel column chromatography separation, collecting an eluent, collecting components with the same secondary metabolites, merging, and carrying out recrystallization treatment to obtain the eleutheroside E; 3, carrying out vacuum concentration and extraction on the fermentation liquor, combining extract liquor, carrying out silica gel column chromatography separation, collecting eluent, and collecting components which are the same as secondary metabolites to obtain a crude product; and carrying out silica gel column chromatography separation, collecting the eluent, merging the same components, and carrying out recrystallization treatment for multiple times to obtain the oleanolic acid. The method is used for producing eleutheroside E and oleanolic acid through microbial fermentation.

The invention discloses a composition for promoting skeletal muscle injury regeneration and application thereof, and belongs to the technical field of medicines. In order to solve the technical problems that in the prior art, the skeletal muscle injury regeneration promotion effect of nutrients is insufficient, and the new application of eleutheroside B in the field is undefined, the invention provides a composition for promoting skeletal muscle injury regeneration, and the composition effectively shortens the regeneration time of skeletal muscle after chemical injury or mechanical injury and improves the skeletal muscle regeneration effect. The maturity of new muscle fibers is improved; the composition can be in oral dosage forms such as instant powder, oral liquid or tablets, can take effect on the day of injury, does not need local injection, is convenient to use, and is suitable for preparing functional food or formula food for special medical purposes for shortening the regeneration time of skeletal muscle after injury and improving the maturity of new muscle fibers. And a new technical scheme is provided for nutrition intervention of skeletal muscle injury.

This invention discloses a cultivation substrate for promoting the growth of Acanthopanax senticosus seedlings and its application, belonging to the field of plant cultivation technology. The cultivation substrate for promoting the growth of Acanthopanax senticosus seedlings provided by this invention uses perlite and peat as a framework to construct a loose, breathable, water-retaining, and fertilizer-retaining physical structure. By adding Bacillus proteolyticus bacterial solution, its growth-promoting, antagonistic, and organic matter-decomposing effects are utilized to improve the rhizosphere microecology. Combined with controlled-release fertilizer, precise nutrient supply is achieved. These three elements synergistically optimize the water, fertilizer, air, and heat environment and microbial community in the rhizosphere of Acanthopanax senticosus seedlings, promoting nutrient absorption and metabolism, effectively improving photosynthetic efficiency and antioxidant enzyme (SOD, POD) activity; and significantly promoting the accumulation of medicinal components. The yield of eleutheroside E in the roots can reach 13.93 times that of the control group, and the yield of syringin in the leaves can reach 2279.82 μg, providing an efficient solution for cultivating high-quality, robust seedlings.

The invention provides a method for determining the content of multiple components in an Aidi preparation, and belongs to the technical field of medicines. The Aidi preparation is prepared from traditional Chinese medicinal materials including ginseng, cantharides, astragalus membranaceus and acanthopanax. The content determination method comprises the following steps: simultaneously determining cantharidin, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rd, isofraxidin, syringin, formononetin, astragaloside, chlorogenic acid, eleutheroside E, calycosin-7-glucoside, astragaloside II, neochlorogenic acid, cryptochlorogenic acid and eleutheroside E1 in the Aidi preparation by adopting liquid chromatography-mass spectrometry; the composition is prepared from effective components such as ginsenoside F3, ginsenoside Rg2, azelaic acid and the like. Compared with the prior art, the method has the advantages that the contents of the 20 effective components in the Aidi preparation are simultaneously measured under the same chromatographic condition through liquid chromatography-mass spectrometry, the method is convenient and rapid, the requirement for product quality detection is greatly improved, the method is more suitable for quality control of drugs in the future, and the drug quality and the clinical drug effect are guaranteed.