19 results about "Ginsenoside Rc" patented technology

The present invention relates to the technical field of determination of ingredients in Chinese patent medicines, and in particular to a method for detecting ginsenoside content and an application thereof. The present invention utilizes a multiple heart-cutting two-dimensional liquid chromatography tandem charged aerosol detector method to detect the content of ginsenosides including notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, 24(R)-pseudoginsenoside F11, ginsenoside Rf, ginsenoside Ra2, ginsenoside Rb1, ginsenoside Rg2, ginsenoside Rh1, ginsenoside Rc, ginsenoside Ro, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rd, ginsenoside Rg3(S), and ginsenoside Rg3(R). The method has the advantages of accuracy, high sensitivity, and strong specificity, is suitable for determining the content of ginsenosides in different Chinese patent medicines containing Panax genus Chinese medicines, and can be used reliably, comprehensively, and accurately for quality control of ginsenosides in Chinese patent medicines containing Panax genus Chinese medicines.

The invention discloses application of ginsenoside Rc and a derivative or salt thereof in preparation of a medicine for preventing and / or treating early lung metastasis of Lewis lung cancer and / or combining other tumors. The ginsenoside Rc shows a better effect of preventing and treating early lung metastasis of Lewis lung cancer and higher safety than cis-platinum in a tumor-bearing mouse model, and no adverse reaction is found.

The invention belongs to the technical field of traditional Chinese medicines, and provides a pharmaceutical composition containing an effective component of American ginseng, a pharmaceutical preparation and application of the pharmaceutical composition and the pharmaceutical preparation in treating chronic obstructive pulmonary diseases. The pharmaceutical composition is prepared from the following components in parts by weight: 1 to 2 parts of ginsenoside Rg1, 1 to 2 parts of ginsenoside Rc, 0.5 to 1 part of oleanolic acid, 0.1 to 0.5 part of notoginsenoside R1 and 0.05 to 0.1 part of notoginsenoside Fe. Specific American ginseng saponin components are compounded, and the dosage of each component is optimized, so that the composition has remarkable effects of protecting the lung function of a COPD mouse caused by cigarette smoke exposure, inhibiting expression of inflammatory factors and relieving in-vivo oxidative stress injury, the repairing effect is remarkable, and in order to improve the effect of American ginseng in treatment of COPD, the composition has a good application prospect. And an important basis is provided for fully exerting the effects of all active components in the American ginseng.

The present application relates to the technical field of compound analysis, and in particular to a quality analysis method of ginseng medicinal materials, comprising the following steps: S100, preparation of a control solution: taking ginsenoside Rg1 control product, ginsenoside Re control product, ginsenoside Rf control product, ginsenoside Rb1 control product, ginsenoside Rc control product, ginsenoside Rb2 control product, ginsenoside Rd control product, 3-O-feruloylquinic acid control product, 4-coumaric acid control product, 8-O-4 / 8-O-4 dehydrogenated tri-ferulic acid control product, and adding methanol to prepare a mixed solution, and shaking to obtain the control solution. The present application synchronously monitors two components of saponins and phenolic acids under one detection method, monitors ten component contents of seven saponin components and three polyphenol components, obtains characteristic spectra of saponin components and polyphenol components, and the monitoring quality is more comprehensive and efficient.

This invention belongs to the field of genetic engineering and biotechnology, specifically relating to a β-glucosidase PO3 for the conversion of rare ginsenosides, its preparation method, and its applications. This invention obtains β-glucosidase PO3 by PCR amplification using genomic DNA of *Xanthomonas pseudoepiplo* TY3-10 as a template. The results of the examples show that this enzyme can not only convert total ginsenosides into rare ginsenosides F2, C-O, C-Mx1, and C-Mc1, but also convert ginsenoside Rb1 into F2, ginsenoside Rb2 into C-O, ginsenoside Rb3 into C-Mx1, and ginsenoside Rc into C-Mc1; it also exhibits excellent catalytic performance and high expression efficiency, possessing extremely high application value and commercial prospects.

The invention discloses an SNP molecular marker, a KASP primer, a kit and a method for detecting the content character of ginsenoside Rc in panax notoginseng, and elaborates the practical application of the kit in breeding of the content character of ginsenoside Rc in panax notoginseng. The SNP molecular marker involved in the invention is SNP-218351403, the marker is located on a chromosome 1 (Chr1), and the specific position is the 218351403 basic group. In addition, the base mutation type of the SNP marker is G / A. According to the invention, a set of KASP (Kompeste Allele Specific PCR) primer combination is developed, and the combination can be used for accurately distinguishing pseudo-ginseng plant individuals with high ginsenoside Rc content and pseudo-ginseng plant individuals with low ginsenoside Rc content. By using the primer combination, the molecular-assisted marker breeding technology can be effectively applied to the breeding process of the panax notoginseng plant, the operation is simple and convenient, the cost is lower, the breeding period of a new variety is shortened, and the primer combination can be used for long-term breeding tracking and variety tracing. Therefore, the method disclosed by the invention is of great significance in accelerating precise breeding of the panax notoginseng with high ginsenoside Rf content.

The application provides a multi-component quantitative analysis method of anti-aging tablets, acetonitrile-0.1% formic acid aqueous solution is used as a mobile phase, an HSS T3 chromatographic column is used for target compound separation, and 13 active components in the anti-aging tablets are simultaneously determined in an MRM mode by using an electrospray ionization triple quadrupole mass spectrometer, the linear range of the 13 active components is 0.4-100 ng / mL, the linear range of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf and ginsenoside Rg1 is 4-1000 ng / mL, the method has high sensitivity, strong specificity and good reproducibility, and realizes simultaneous quantitative analysis of the 13 active components in the anti-aging tablets.

The application discloses application of ginsenoside Rc and derivatives or salts thereof in preparation of a medicine for preventing and / or treating early lung metastasis of Lewis lung cancer and / or combination with other tumors. The ginsenoside Rc of the application shows better prevention and treatment effects on early lung metastasis of Lewis lung cancer and higher safety than cisplatin in a tumor-bearing mouse model, and no adverse reactions are found.

The application of ginseng natto compound ferment in preparation of Alzheimer's disease medicine relates to the technical field of medicine, and solves the technical problems of safety risk and the like of the existing Alzheimer's disease prevention and treatment medicine.The ginseng natto compound ferment comprises natto kinase, soybean glycoside, soybean glycoside, genistin, daidzein, genistein, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rc.The soybean is soaked in ginseng extract solution until the soybean absorbs the ginseng extract solution completely;the soaked soybean is sterilized as a fermentation substrate;the bacillus subtilis is inoculated, shaken and continuously cultured;and the product after the culture is dried, crushed and sieved to obtain the ginseng natto compound ferment.The application provides a new solution for upgrading of functional food to neuroprotective agent.

The application discloses a method for preparing rare ginsenoside CK by gene combination transformation and application. Bacillus subtilis str. 168)and bifidobacterium ( Bifidobacterium breve 689b)can efficiently transform ginsenoside Rc into rare ginsenoside CK in vitro by using the glycosidase produced by the genes of the bacteria.

The present invention discloses a recombinant yeast strain and its application in the biotransformation of ginsenoside of panaxadiol type, belonging to the field of genetic engineering. The constructed recombinant Pichia pastoris of the present invention overexpresses β-glucosidase β-GC derived from Aspergillus tubingensis, and the amino acid sequence of the β-glucosidase is shown as SEQ ID NO.1 or SEQ ID NO.3; the β-glucosidase has the ability to specifically hydrolyze ginsenoside Rb1, ginsenoside Rc, ginsenoside Rb2 and ginsenoside Rb3 into ginsenoside Rd, and can hydrolyze ginsenoside F2 into ginsenoside CK. The β-glucosidase prepared by the present invention can be widely applied in the fields of food, medicine, bioenergy and the like.

The invention discloses application of a ginseng and natto compound fermentation product in preparation of medicines for treating Alzheimer's disease, relates to the technical field of medicines, and solves the technical problems of safety risk and the like of the existing medicines for preventing and treating Alzheimer's disease. The ginseng and natto compound fermentation product is prepared from nattokinase, daidzein, daidzein, genistin, daidzein, genistein, ginsenoside Rg1, ginsenoside Rb1 and ginsenoside Rc. Soaking the soybeans in the ginseng extracting solution until the soybeans completely absorb the ginseng extracting solution; the soaked soybeans are sterilized to serve as a fermentation substrate; and inoculating bacillus subtilis, uniformly shaking, carrying out continuous culture, and drying, crushing and sieving the cultured product to obtain the ginseng and natto compound fermentation product. The invention provides a brand new solution for upgrading functional foods to neuroprotective agents.

The invention discloses application of ginsenoside Rc in preparation of a product for resisting skin high temperature and light damage. The skin high temperature and light damage resistance is reflected in the following aspects: resisting inflammation induced by high temperature; the inflammation induced by high temperature and ultraviolet light products FICZ is resisted. Research results show that ginsenoside Rc can significantly reduce the induction effect of FICZ on IL-6 and IL-8 in a high-temperature environment, and provides a potential protection mechanism for resisting inflammatory reactions aggravated by the high-temperature condition. The discovery not only provides a theoretical basis for application of ginsenoside Rc in defense of skin inflammation induced by environmental factors, but also reveals a potential anti-inflammatory mechanism of ginsenoside Rc on a cellular level, and provides a scientific basis for subsequent application and development in the field of skin care products.

The present invention belongs to the technical field of modernization of traditional Chinese medicine, and in particular to a composition, application and product for preventing or treating chronic atrophic gastritis. The composition for preventing or treating chronic atrophic gastritis provided by the present invention is composed of isobarbin, ginsenoside Re, naringin, ginsenoside Rc and dioscorea lignin. The composition provided by the present invention can inhibit the PI3K / AKT pathway of inflammatory cells, activate the MAPK pathway of gastric mucosal epithelial cells, inhibit the expression of inflammatory factors such as IL-6 and TNF-α and promote GAST gastrin gene expression, thereby inhibiting inflammation, promoting gastrin secretion, enhancing gastric motility and promoting gastric mucosal epithelial cell repair, and achieving the effect of preventing or treating chronic atrophic gastritis. In addition, the components of the composition of the present invention are abundant in the medicinal and edible products of the Araliaceae family and the Rutaceae family, have low toxic and side effects, and show good application prospects in the preparation of drugs for preventing or treating chronic atrophic gastritis.

The invention relates to a ginsenoside composition with an effect of regulating blood viscosity as well as a preparation method and application of the ginsenoside composition, and belongs to the technical field of biology. The invention provides a ginsenoside composition. The ginsenoside composition comprises at least two of ginsenoside Rc, ginsenoside Rb2, ginsenoside Rb3, ginsenoside Rd, ginsenoside Rg6, ginsenoside F4, ginsenoside S-Rg3, ginsenoside R-Rg3, ginsenoside PPT, ginsenoside Rk1, ginsenoside Rg5 and ginsenoside Rh2. The ginsenoside composition provided by the invention has the efficacy of improving body weight rise, liver injury, lipid metabolism disorder, insulin level rise and oxidative stress caused by high-fat and high-sugar diet, and has the efficacy of improving blood viscosity rise, blood coagulation time reduction and blood coagulation factor level improvement caused by acute blood stasis; the effects of improving liver injury, blood fat, blood sugar and lipid metabolism disorder, regulating blood viscosity and resisting oxidation can be achieved, and the compound can be expected to be developed into an active raw material to be applied to the fields of biological medicine, cosmetics, food, health care products and the like.

The invention discloses an application of ginsenoside Rc or a salt thereof in preparation of a medicine for preventing and / or treating colorectal tumor and / or combining other tumors. The ginsenoside Rc disclosed by the invention shows a relatively good colorectal cancer treatment effect and good safety in an MC38 tumor-bearing mouse model, and no adverse reaction is found.

The invention belongs to the technical field of traditional Chinese medicine modernization, and particularly relates to a composition for preventing or treating chronic atrophic gastritis, application and a product. The composition for preventing or treating the chronic atrophic gastritis, provided by the invention, consists of isorhoifolin, ginsenoside Re, naringin, ginsenoside Rc and diosmetin. The composition provided by the invention can inhibit an inflammatory cell PI3K / AKT pathway, activate a gastric mucosa epithelial cell MAPK pathway, inhibit expression of inflammatory factors such as IL-6 and TNF-alpha and promote GAST gastrin gene expression, so that inflammation is inhibited, gastrin secretion is promoted, gastric motility is enhanced, gastric mucosa epithelial cell repair is promoted, and the effect of preventing or treating chronic atrophic gastritis is achieved. In addition, the components of the composition disclosed by the invention are rich in araliaceae and rutaceae medicinal and edible products and small in toxic and side effects, and the composition shows a good application prospect in preparation of medicines for preventing or treating chronic atrophic gastritis.

The present invention discloses a method for preparing a ginsenoside Rc reference standard, comprising: a) adding an ethanol solution to a sample of ginsenoside components, stirring and ultrasonicating the sample until completely dissolved, filtering the sample through a membrane, loading the sample onto a reverse-phase chromatography column with a large-particle packing, performing linear gradient elution with an ethanol solution, and analyzing and combining the target fractions containing ginsenoside Rc to obtain a crude fraction; b) loading the crude fraction onto a reverse-phase chromatography column with a small-particle packing, performing isocratic elution with an ethanol solution, and analyzing and combining the target fractions containing ginsenoside Rc to obtain a crude ginsenoside Rc solution; c) loading the crude product solution onto a hydrophilic chromatography column with a small-particle packing, performing isocratic elution with an ethanol-water solution to obtain a pure ginsenoside Rc solution; and d) concentrating and removing alcohol from the pure product solution, and freeze-drying the solution to obtain a ginsenoside Rc reference standard. The ginsenoside Rc monomer obtained by the present invention has a purity exceeding 98%, and the process is simple, easy to operate, and low-cost. No toxic or hazardous solvents are used in the preparation process.

The invention provides a method for determining the content of multiple components in an Aidi preparation, and belongs to the technical field of medicines. The Aidi preparation is prepared from traditional Chinese medicinal materials including ginseng, cantharides, astragalus membranaceus and acanthopanax. The content determination method comprises the following steps: simultaneously determining cantharidin, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc, ginsenoside Rd, isofraxidin, syringin, formononetin, astragaloside, chlorogenic acid, eleutheroside E, calycosin-7-glucoside, astragaloside II, neochlorogenic acid, cryptochlorogenic acid and eleutheroside E1 in the Aidi preparation by adopting liquid chromatography-mass spectrometry; the composition is prepared from effective components such as ginsenoside F3, ginsenoside Rg2, azelaic acid and the like. Compared with the prior art, the method has the advantages that the contents of the 20 effective components in the Aidi preparation are simultaneously measured under the same chromatographic condition through liquid chromatography-mass spectrometry, the method is convenient and rapid, the requirement for product quality detection is greatly improved, the method is more suitable for quality control of drugs in the future, and the drug quality and the clinical drug effect are guaranteed.