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Method for extracting and separating total saponins of panax ginseng from american ginseng

A technology of total ginsenosides and American ginseng is applied in the directions of medical preparations containing active ingredients, pharmaceutical formulas, plant/algae/fungus/moss ingredients, etc., and can solve the problems of dark color, complicated steps, low yield and purity, etc.

Active Publication Date: 2012-11-14
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent application No. 03127613.X discloses a method for extracting and refining total ginseng saponins from American ginseng fruit. American ginseng fruit is subjected to water extraction, macroporous adsorption resin for impurity removal, decolorization, and drying. Although this method is simple, the color is relatively Deep, low yield and purity
The purity of total saponins obtained by this method is higher, but the steps are loaded down with trivial details and the yield is low
Chinese patent application number 200610014229.6 discloses a preparation method of total saponins of American ginseng, adding American ginseng into a solvent, adjusting the base and then extracting, removing impurities and decolorizing with macroporous anion exchange resin, this method may change the structure of some ginsenosides
Chinese patent application number 200610109414.3 discloses a preparation method for extracting total saponins from American ginseng or American ginseng stems and leaves. cumbersome

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] a. Extraction: Weigh 10 kg of American ginseng coarse powder, add 60L of 70% ethanol solution each time, heat and reflux extraction 3 times, each time for 3 hours, soak for 60 minutes for the first time, and filter; the filtrates are combined, concentrated under reduced pressure until there is no alcohol smell, and constant volume 20L, to obtain a concentrate;

[0045] b. Water precipitation: dilute the concentrated solution with water to 100L, shake well, place in the refrigerator for 12 hours, filter, discard the filter residue, and obtain the extract;

[0046] c. The first purification: Weigh 6.25kg of HPD100 macroporous resin, put it into a chromatographic column with a diameter of 9.4cm, a height of 122cm, and a volume of 8.5L, and clean it with 95% ethanol and distilled water; load the extract , the adsorption flow rate is 38L / h, saturated for 3h; use 42.5L of distilled water to elute to remove sugar, and the elution flow rate is 102L / h; use 60L20% ethanol solutio...

Embodiment 2

[0051] a. Extraction: Weigh 15Kg of American ginseng coarse powder, add 180L of 30% ethanol solution each time, heat and reflux for extraction twice, each time for 1h, soak for 90min for the first time, filter; combine the filtrates, concentrate under reduced pressure until there is no alcohol smell, and set the volume to 30L , to obtain a concentrated solution;

[0052] b. Water precipitation: dilute the concentrated solution with water to 90L, shake well, place in the refrigerator for 24 hours, filter, discard the filter residue, and obtain the extract;

[0053] c. The first purification: Weigh 15 kg of HPD100A macroporous adsorption resin, put it into a chromatographic column with a diameter of 20 cm, a height of 60 cm, and a volume of 19 L, and clean it with 95% ethanol and distilled water; load the extract, and absorb The flow rate is 171L / h 9 times column volume / hour, saturated for 1h; use 190L of distilled water to elute to remove sugar, and the elution flow rate is 86L...

Embodiment 3

[0058] a. Extraction: Weigh 20 kg of American ginseng coarse powder, add 180L of 50% ethanol solution each time, heat and reflux for extraction 3 times, each time for 2 hours, soak for 75 minutes for the first time, filter; combine the filtrates, concentrate under reduced pressure until there is no alcohol smell, and set the volume to 20L , to obtain a concentrated solution;

[0059] b. Water precipitation: dilute the concentrated solution with water to 80L, shake well, place in the refrigerator for 18 hours, filter, discard the filter residue, and obtain the extract;

[0060] c. The first purification: Weigh 13Kg of HPD300 macroporous adsorption resin, put it into a chromatographic column with a diameter of 14cm, a height of 112cm, and a volume of 17L, and clean it with 95% ethanol and distilled water; load the extract, and absorb The flow rate is 102L / h, saturated for 2h; use 127.5L distilled water to elute sugar, the elution flow rate is 102L / h; use 119L20% ethanol solution...

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Abstract

The invention discloses a method for extracting and separating total saponins of panax ginseng from american ginseng. The method employs a series of high-efficiency extraction, separation and purification technical measures of alcohol-backflow extracting, water precipitating, purifying by a macroporous adsorption resin, decolorizing with an ion exchange resin, etc. The obtained total saponins of panax ginseng are white powder and comprise ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd, wherein the total purity of ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 is more than 74.0%. The production process is simple and practical, has no pollution, and is suitable for large-scale production.

Description

technical field [0001] The invention relates to a method for extracting and separating total ginsenosides from American ginseng. Background technique [0002] American ginseng (Panax quinquefolium L.) is a perennial herb of the Araliaceae family (Araliaceae), native to the eastern United States and Canada. Also known as American ginseng, American ginseng, American ginseng and Guangzhou ginseng. In my country, it was first recorded in "Compendium of Materia Medica Supplements", and has a medicinal history of more than 200 years. It has the effects of nourishing lung yin, clearing deficiency fire, promoting body fluid and quenching thirst, etc., and can treat chronic cough due to lung deficiency, blood loss, dry throat and thirst, deficiency heat and tiredness. "Medical Hearts of Chinese Ginseng and Western Records" contains: "American ginseng is cool in nature and nourishing. Anyone who wants to use ginseng without the warming effect of ginseng can replace it." It is known...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P39/00A61P25/00A61P9/10A61P35/00A61P37/04A61K125/00
Inventor 王宗权贾继明宋剑侯素云马静杨超
Owner HEBEI YILING MEDICINE INST
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