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Panax notoginseng flower total saponin, and preparation method and application thereof

A technology of Panax notoginseng saponins and Panax notoginseng flowers is applied in the field of biomedicine to achieve the effects of less toxic and side effects, simple operation and high yield

Inactive Publication Date: 2020-03-24
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no patent literature report on the preparation of total saponins in Panax notoginseng by ultra-high pressure extraction method

Method used

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  • Panax notoginseng flower total saponin, and preparation method and application thereof
  • Panax notoginseng flower total saponin, and preparation method and application thereof
  • Panax notoginseng flower total saponin, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1: the preparation method of Panax notoginseng saponins is as follows:

[0033] Take 100g of Panax notoginseng flower powder, add 2L of ethanol aqueous solution with a volume fraction of 70%, and extract at room temperature by ultra-high pressure extraction method, the pressure is 300MPa, and the time is 3min. After the extract is filtered, it is concentrated and dried under reduced pressure to obtain three Seven flower total saponins (55.6g).

[0034] The content of total saponins was determined by ultraviolet spectrophotometry. The specific steps are as follows: Precisely draw the reference solution of Panax notoginseng saponins and the same volume of Panax notoginseng total saponins samples into a test tube with a stopper. After the solvent is evaporated, add 0.2mL 5% vanillin-glacial acetic acid reagent, 0.8mL perchloric acid, heated in a water bath at 60°C for 15min, cooled in an ice-water bath, added 5mL of glacial acetic acid and shaken well, analyzed ...

Embodiment 2

[0038] Embodiment 2: The preparation method of Panax notoginseng saponins is as follows:

[0039] Take 100g of Panax notoginseng flower powder, add 6L volume fraction of 60% methanol aqueous solution, extract at room temperature by ultra-high pressure extraction method, the pressure is 500MPa, the time is 6min, after the extract is filtered, concentrated and dried under reduced pressure to obtain Panax notoginseng Total flower saponins (60.4g); measured by the same ultraviolet spectrophotometry method as in Example 1, the mass percent content of saponins in the total saponins of Panax notoginseng flower is 68.2%.

[0040]Adopt the same high-performance liquid chromatography of embodiment 1 to measure, and detection result shows, contains notoginseng saponin Fc, ginsenoside Rc, ginsenoside Rb in the total saponins of notoginseng flower 2 , Ginsenoside Rb 3 , ginsenoside Rd, each mass percentage content is: the sum of notoginsenoside Fc and ginsenoside Rc, ginsenoside Rb 2 , G...

Embodiment 3

[0041] Embodiment 3: the preparation method of Panax notoginseng saponins is as follows:

[0042] Take 100g of Panax notoginseng flower powder, add 4L of water, at room temperature, use the ultra-high pressure extraction method to extract, the pressure is 400MPa, the time is 4min, after the extract is filtered, concentrated and dried under reduced pressure to obtain the total saponins of Panax notoginseng (65.3 g) The mass percent content of total saponins was determined to be 70.2% by using the same ultraviolet spectrophotometric method as in Example 1.

[0043] Adopt the same high-performance liquid chromatography of embodiment 1 to measure, and detection result shows, contains notoginseng saponin Fc, ginsenoside Rc, ginsenoside Rb in the total saponins of notoginseng flower 2 , Ginsenoside Rb 3 , ginsenoside Rd, each mass percentage content is: the sum of notoginsenoside Fc and ginsenoside Rc, ginsenoside Rb 2 , Ginsenoside Rb 3 , ginsenoside Rd content were 10.3%, 3.2%,...

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Abstract

The invention discloses a Panax notoginseng flower total saponin, and a preparation method and an application thereof, and belongs to the field of biological medicines. The Panax notoginseng flower total saponin contains notoginsenoside Fc, ginsenoside Rc, ginsenoside Rb2, ginsenoside Rb3 and ginsenoside Rd in any proportion, and is prepared from Panax notoginseng flowers through the processes ofultrahigh-pressure extraction, filtration, concentration, drying and the like. The preparation method is carried out at normal temperature, is simple to operate and has a high efficiency, and the obtained Panax notoginseng flower total saponin has strong hydroxyl radical and DPPH free radical scavenging capacity, and can be used for preparing antioxidant drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a total saponin of Panax notoginseng and its preparation method and its application in the preparation of anti-oxidation medicine. Background technique [0002] Oxidative stress has a very important impact on human aging and health. Excessive free radicals caused by various factors can trigger and aggravate many diseases, such as Alzheimer's disease, cardiovascular disease, malignant tumors, etc. Therefore, the application of antioxidants To maintain human health has a wide range of needs. Due to the potential risks of carcinogenicity and toxicity of synthetic antioxidants, it is an inevitable trend in the development of antioxidants in today's food science to find safe, effective, and non-toxic natural antioxidants. important raw materials. [0003] Panax notoginseng Panax notoginseng (Burk.) The dried flower buds of F. H. Chen are mainly produced in Yunnan P...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61K31/704A61P39/06A61P9/00A61P35/00A61P17/00A61P9/12A61P3/10A61P25/00
CPCA61K36/258A61K31/704A61P39/06A61P9/00A61P35/00A61P17/00A61P9/12A61P3/10A61P25/00A61K2236/37A61K2236/51
Inventor 潘俊曲媛崔秀明刘迪秋唐美玲
Owner KUNMING UNIV OF SCI & TECH
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