The invention provides a culture base material for culturing toadstool, and belongs to the technical field of planting of edible mushrooms. The culture base material is prepared from the following raw materials in parts by weight of 30-50 parts of saw dust, 10-20 parts of wheat bran, 10-15 parts of bagasse, 5-15 parts of earthworm cast, 3-6 parts of distillers' grains, 10-15 parts of straw, 10-15 parts of humus soil, 2-4 parts of potassium dihydrogen phosphate and 1-2 parts of plaster. The culture base material for culturing the toadstool, prepared from the saw dust, the wheat bran, the bagasse, the earthworm cast, the distillers' grains, the straw, the humus soil, the potassium dihydrogen phosphate and the plaster has the characteristics of being few in infectious microbes, high in success rate, and high in yield of the toadstool. Compared with a culture base material disclosed by a patent literature 1, the success rate of the culture base material disclosed by the invention can reach 95% or above (being 10-15% higher than the patent literature 1), and the average yield of single culture base materials for culturing the toadstool is 50% or above higher than the patent literature 1.