Separation method of monoterpene and saponins components in traditional Chinese medicine composition vegetable drug midbody

A separation method and composition technology, applied in the direction of steroids, chemical instruments and methods, sugar derivatives, etc., can solve problems such as complex prescriptions and unclear basic mechanism of action of active substances

Active Publication Date: 2014-10-01
HEBEI YILING MEDICINE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the prescription of this medicine is complicated. Although a lot of research has been done on its mechanism of action over the years, it is limited to finished products, and the basis of its active substances and its mechanism of action are still unclear.

Method used

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  • Separation method of monoterpene and saponins components in traditional Chinese medicine composition vegetable drug midbody

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] The separation method of monoterpene and saponin components in the botanical medicine intermediate of the Chinese medicine composition, the weight of the botanical medicine in the Chinese medicine composition is as follows: ginseng 6kg, red peony root 5kg, jiangxiang 2kg, sandalwood 2kg, fried jujube seed 5kg, including The following steps:

[0092] Extraction: Weigh 4 kg of the above-mentioned botanical drug intermediate, dissolve it with 20 times the amount of water, then extract it with petroleum ether, ethyl acetate, and n-butanol successively for 3 times, and recover the solvent under reduced pressure;

[0093] Macroporous adsorption resin purification: Use D101 macroporous resin to roughly separate the n-butanol extract, elute with water, 30% ethanol, 70% ethanol, and 95% ethanol in sequence, and collect 30% and 70% of the eluent after concentration and drying. % ethanol elution site;

[0094] Take the fraction eluted with 30% ethanol, use a silica gel chromatogr...

Embodiment 2

[0106] The method for separating monoterpene and saponin components in the botanical intermediates of the Chinese medicine composition, the weight of the botanicals in the Chinese medicine composition is as follows: ginseng 6kg, red peony root 4kg, jiangxiang 3kg, sandalwood 2kg, fried jujube seed 4kg, including the following step:

[0107] Extraction: Weigh 4 kg of the above-mentioned botanical drug intermediate, dissolve it with 20 times the amount of water, then extract it with petroleum ether, ethyl acetate, and n-butanol successively for 3 times, and recover the solvent under reduced pressure;

[0108] Macroporous adsorption resin purification: Use D101 macroporous resin to roughly separate the n-butanol extract, elute with water, 30% ethanol, 70% ethanol, and 95% ethanol in sequence, and collect 30% and 70% of the eluent after concentration and drying. % ethanol elution site;

[0109] Take the fraction eluted with 30% ethanol, use a silica gel chromatography column, and...

Embodiment 3

[0121] The method for separating monoterpene and saponin components in the botanical intermediates of the Chinese medicine composition, the weight of the botanicals in the Chinese medicine composition is as follows: ginseng 3kg, red peony root 7kg, jiang xiang 4kg, sandalwood 3kg, fried jujube seed 3kg, including the following step:

[0122] Extraction: Weigh 4 kg of the above-mentioned botanical drug intermediate, dissolve it with 20 times the amount of water, then extract it with petroleum ether, ethyl acetate, and n-butanol successively for 3 times, and recover the solvent under reduced pressure;

[0123]Macroporous adsorption resin purification: Use D101 macroporous resin to roughly separate the n-butanol extract, elute with water, 30% ethanol, 70% ethanol, and 95% ethanol in sequence, and collect 30% and 70% of the eluent after concentration and drying. % ethanol elution site;

[0124] Take the fraction eluted with 30% ethanol, use a silica gel chromatography column, and...

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Abstract

The invention discloses a separation method of monoterpene and saponins components in a traditional Chinese medicine composition vegetable drug midbody. The method comprises the following steps of: extracting and purifying by macroporous adsorption resin to obtain 30 percent and 70 percent of ethanol elution parts, separating the 30 percent of ethanol elution part to obtain a white paeony root R1 pure product and a paeoniflorin pure product; separating the 70 percent of ethanol elution part to obtain a paeoniflorin lactone pure product, a peony ketone glycosides pure product, a benzoyl paeoniflorin pure product, a gallate paeoniflorin pure product, a methyl paeoniflorin pure product, a ginsenoside Rg1 pure product, a ginsenoside Rc pure product, a ginsenoside Rb1 pure product, a ginsenoside Rb2 pure product and the like. According to the separated effective component, the administration dosage of the drugs can be shortened, and the quality of the drugs can also be guaranteed.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine preparations, and in particular relates to a method for separating active ingredients of traditional Chinese medicines. Background technique [0002] Coronary heart disease angina pectoris is a disease that seriously endangers human health and has become one of the three major causes of death in humans. Angina pectoris is the most common clinical type of coronary heart disease, which not only brings great suffering to patients, but also can cause serious consequences. Most scholars report that the annual mortality rate of angina pectoris patients is about 4%, and 30% of patients will die within 8 years. [0003] Our company uses the theory of collateral disease in traditional Chinese medicine to study the pathological mechanism of chest obstruction and heart pain, and selects Qi-invigorating and worm dredging collaterals to develop a new traditional Chinese medicine preparation for the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/08C07J17/00
Inventor 付强王曙宾
Owner HEBEI YILING MEDICINE INST
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