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Method for detecting Jingfang granules

A detection method and particle prevention technology, applied in the field of Jingfang particle detection, can solve the problems of interference, complex components, and decreased drug efficacy.

Active Publication Date: 2014-07-30
九寨沟天然药业股份有限公司
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  • Claims
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Problems solved by technology

But so far, there is no complete set of detection methods for the corresponding detection of its active ingredients, which may lead to unscrupulous manufacturers not strictly following the prescription dosage ingredients in the production of drugs, wantonly reducing high-priced raw materials, resulting in the curative effect of the drug. Significant decline, affecting the safety and effectiveness of drugs, seriously damaging the interests of patients
On the other hand, the medicinal material Bupleurum bupleurum is used in the prescription. Because of its large amount of use and its limited output, its counterfeit product, Bupleurum bupleurum is also used intentionally or unintentionally. However, Bupleurum bupleurum contains bupleurum toxin and acetylbupleurum toxin, these two components are poisonous substances, there have been many poisoning incidents caused by taking medicines mixed with Bupleurum bupleurum, so it is necessary to control the Bupleurum bupleurum
"Shandong Pharmaceutical Industry" in 2003, Volume 22, No. 5, "Supplement to the Inspection Method of Bupleurum 2000 Edition of the Chinese Pharmacopoeia", also discussed the identification and control of Bupleurum bupleuri in the Pharmacopoeia, but the method has obvious differences. Disadvantages: Placed for 24 hours, the detection time is too long, which is not conducive to the control of drug production process and drug quality supervision and inspection; because the content of Bupleurum toxin and acetylbupleurum toxin is relatively low, the sample is only 2μl, and the amount of sample is too small, which affects the test Accuracy; because the content of Bupleurum toxin and acetylbupleurum toxin is relatively low, the observation effect is not good when inspected under ultraviolet light without using color development agent to develop color
What's more important is that the prescription of Jingfang Granules has many medicinal flavors and complex ingredients, and other ingredients will cause confusion and interference

Method used

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  • Method for detecting Jingfang granules
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  • Method for detecting Jingfang granules

Examples

Experimental program
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Effect test

Embodiment 1

[0085] [Prescription] Nepeta 75g Fangfeng 75g Notopterygium 75g Duhuo 75g Bupleurum 75g Peucedanum 75g Chuanxiong 75g Citrus aurantium 75g Poria 75g Bellflower 75g Licorice 25g

[0086] [Preparation method] The above eleven herbs, Nepeta, Fangfeng, Notopterygium, Duhuo, Peucedanum, Chuanxiong and Citrus aurantium extract the volatile oil respectively, and the distilled aqueous solution of Chuanxiong and Citrus aurantium is collected separately; , according to the percolation method under the item of liquid extract and extract, use the above aqueous solution to make 25% ethanol solution as solvent, and carry out percolation; Add water to decoct the three flavors of bellflower, licorice, etc. twice, 1.5 hours each time, combine the decoction, filter, and concentrate the filtrate into a thick paste; combine the decoction and thick paste, mix well, let stand, filter, and store at 80-85°C The filtrate is concentrated into a clear paste with a relative density of 1.30 at a temperatu...

Embodiment 2

[0103] In this embodiment, except that the identification method is different, all the other detection methods are the same as in Example 1, and the specific identification method is as follows:

[0104] (1) Identification of Schizonepeta

[0105] Take 10g of this product, add 50ml of ether, sonicate for 15 minutes, filter, evaporate the filtrate to dryness, add 1ml of ethanol to the residue to dissolve, and use it as the test solution, and take 1g of Nepeta nepeta reference drug, add 50ml of ether, and sonicate for 15 minutes , filtered, the filtrate was evaporated to dryness, and the residue was dissolved by adding 1ml of ethanol. As the medicinal material solution, according to the thin-layer chromatography test, absorb 5 μl of each of the above two solutions, and respectively spot on the same silica gel G thin-layer plate, with petroleum ether: Chloroform:methanol=10:3:2 is the developing agent, develop, take out, dry in the air, spray with 5% vanillin sulfuric acid soluti...

Embodiment 3

[0115] In this embodiment, except that the identification method is different, all the other detection methods are the same as in Example 1, and the specific identification method is as follows:

[0116] (1) Identification of Schizonepeta

[0117] Take 10g of this product, add 50ml of ether, sonicate for 15 minutes, filter, evaporate the filtrate to dryness, add 1ml of ethanol to the residue to dissolve, and use it as the test solution, and take 1g of Nepeta nepeta reference drug, add 50ml of ether, and sonicate for 15 minutes , filtered, the filtrate was evaporated to dryness, and the residue was dissolved by adding ethanol 1ml. As the medicinal material solution, according to the thin-layer chromatography test, draw each 5 μl of the above-mentioned two solutions, respectively point on the same silica gel G thin-layer plate, and use petroleum ether: Chloroform:methanol=11:4:2.5 is developing agent, develops, takes out, dries, sprays with 5% vanillin sulfuric acid solution, is...

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Abstract

The invention relates to a method for detecting Jingfang granules. The method comprises the steps of character identifying, checking and measuring content, wherein the step of identifying comprises identification of schizonepeta, radix angelicae pubescentis, ligusticum wallichii, fructus aurantii and bupleurum longiradiatum; the step of measuring content refers to a step of measuring content of ephedrine hydrochloride. Aiming at the problem that the condition that fewer raw materials are added or corresponding raw materials are not added by illegal manufacturer or a counterfeit drug bupleurum longiradiatum is mixed cannot be monitored because active ingredients of the granules cannot be correspondingly detected by a set of perfect detection method at present, a scientific, reasonable, practical and feasible component identification and content measurement method is formulated, the clinical effects of the Jingfang granules are guaranteed, quality indexes of several medicinal materials are clear, and application of various medicinal materials in the Jingfang granules is ensured, so that the curative effect of the drug is guaranteed. Moreover, the counterfeit drug bupleurum longiradiatum in radix bupleuri can be effectively detected, and a phenomenon that health of patients is endangered by two toxic ingredients, including bupleurotoxin and acetylbupleurotoxin, contained in bupleurum longiradiatum is avoided.

Description

technical field [0001] The invention relates to a method for detecting Jingfang particles. Background technique [0002] Jingfang (Granules) Granules is a variety recorded in the second volume of traditional Chinese medicine preparations in the "Drug Standards of the Ministry of Health". The standard number is WS3-B-0328-90. Peucedanum, Rhizoma Chuanxiong, Citrus aurantium, Poria, Campanulaceae, Licorice are pure traditional Chinese medicine and granules. It has the effects of sweating, relieving the exterior, dispersing wind and dampness. It is clinically used to treat cold, wind and cold, headache and body pain, aversion to cold without sweating, stuffy nose and runny nose, and cough. It is a common medicine currently on the market for treating common cold, wind and cold, headache and body pain, aversion to cold without sweating, nasal congestion and runny nose, and cough. But so far, there is no complete set of detection methods for the corresponding detection of its a...

Claims

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Application Information

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IPC IPC(8): G01N30/90G01N30/02
Inventor 钟茂团黎勇吴诗惠古莉
Owner 九寨沟天然药业股份有限公司
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